summaryrefslogtreecommitdiff
path: root/gnu/packages/bioinformatics.scm
blob: 1a82815e924745baadac211a3b9c2a4f27aead28 (about) (plain)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
474
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500
501
502
503
504
505
506
507
508
509
510
511
512
513
514
515
516
517
518
519
520
521
522
523
524
525
526
527
528
529
530
531
532
533
534
535
536
537
538
539
540
541
542
543
544
545
546
547
548
549
550
551
552
553
554
555
556
557
558
559
560
561
562
563
564
565
566
567
568
569
570
571
572
573
574
575
576
577
578
579
580
581
582
583
584
585
586
587
588
589
590
591
592
593
594
595
596
597
598
599
600
601
602
603
604
605
606
607
608
609
610
611
612
613
614
615
616
617
618
619
620
621
622
623
624
625
626
627
628
629
630
631
632
633
634
635
636
637
638
639
640
641
642
643
644
645
646
647
648
649
650
651
652
653
654
655
656
657
658
659
660
661
662
663
664
665
666
667
668
669
670
671
672
673
674
675
676
677
678
679
680
681
682
683
684
685
686
687
688
689
690
691
692
693
694
695
696
697
698
699
700
701
702
703
704
705
706
707
708
709
710
711
712
713
714
715
716
717
718
719
720
721
722
723
724
725
726
727
728
729
730
731
732
733
734
735
736
737
738
739
740
741
742
743
744
745
746
747
748
749
750
751
752
753
754
755
756
757
758
759
760
761
762
763
764
765
766
767
768
769
770
771
772
773
774
775
776
777
778
779
780
781
782
783
784
785
786
787
788
789
790
791
792
793
794
795
796
797
798
799
800
801
802
803
804
805
806
807
808
809
810
811
812
813
814
815
816
817
818
819
820
821
822
823
824
825
826
827
828
829
830
831
832
833
834
835
836
837
838
839
840
841
842
843
844
845
846
847
848
849
850
851
852
853
854
855
856
857
858
859
860
861
862
863
864
865
866
867
868
869
870
871
872
873
874
875
876
877
878
879
880
881
882
883
884
885
886
887
888
889
890
891
892
893
894
895
896
897
898
899
900
901
902
903
904
905
906
907
908
909
910
911
912
913
914
915
916
917
918
919
920
921
922
923
924
925
926
927
928
929
930
931
932
933
934
935
936
937
938
939
940
941
942
943
944
945
946
947
948
949
950
951
952
953
954
955
956
957
958
959
960
961
962
963
964
965
966
967
968
969
970
971
972
973
974
975
976
977
978
979
980
981
982
983
984
985
986
987
988
989
990
991
992
993
994
995
996
997
998
999
1000
1001
1002
1003
1004
1005
1006
1007
1008
1009
1010
1011
1012
1013
1014
1015
1016
1017
1018
1019
1020
1021
1022
1023
1024
1025
1026
1027
1028
1029
1030
1031
1032
1033
1034
1035
1036
1037
1038
1039
1040
1041
1042
1043
1044
1045
1046
1047
1048
1049
1050
1051
1052
1053
1054
1055
1056
1057
1058
1059
1060
1061
1062
1063
1064
1065
1066
1067
1068
1069
1070
1071
1072
1073
1074
1075
1076
1077
1078
1079
1080
1081
1082
1083
1084
1085
1086
1087
1088
1089
1090
1091
1092
1093
1094
1095
1096
1097
1098
1099
1100
1101
1102
1103
1104
1105
1106
1107
1108
1109
1110
1111
1112
1113
1114
1115
1116
1117
1118
1119
1120
1121
1122
1123
1124
1125
1126
1127
1128
1129
1130
1131
1132
1133
1134
1135
1136
1137
1138
1139
1140
1141
1142
1143
1144
1145
1146
1147
1148
1149
1150
1151
1152
1153
1154
1155
1156
1157
1158
1159
1160
1161
1162
1163
1164
1165
1166
1167
1168
1169
1170
1171
1172
1173
1174
1175
1176
1177
1178
1179
1180
1181
1182
1183
1184
1185
1186
1187
1188
1189
1190
1191
1192
1193
1194
1195
1196
1197
1198
1199
1200
1201
1202
1203
1204
1205
1206
1207
1208
1209
1210
1211
1212
1213
1214
1215
1216
1217
1218
1219
1220
1221
1222
1223
1224
1225
1226
1227
1228
1229
1230
1231
1232
1233
1234
1235
1236
1237
1238
1239
1240
1241
1242
1243
1244
1245
1246
1247
1248
1249
1250
1251
1252
1253
1254
1255
1256
1257
1258
1259
1260
1261
1262
1263
1264
1265
1266
1267
1268
1269
1270
1271
1272
1273
1274
1275
1276
1277
1278
1279
1280
1281
1282
1283
1284
1285
1286
1287
1288
1289
1290
1291
1292
1293
1294
1295
1296
1297
1298
1299
1300
1301
1302
1303
1304
1305
1306
1307
1308
1309
1310
1311
1312
1313
1314
1315
1316
1317
1318
1319
1320
1321
1322
1323
1324
1325
1326
1327
1328
1329
1330
1331
1332
1333
1334
1335
1336
1337
1338
1339
1340
1341
1342
1343
1344
1345
1346
1347
1348
1349
1350
1351
1352
1353
1354
1355
1356
1357
1358
1359
1360
1361
1362
1363
1364
1365
1366
1367
1368
1369
1370
1371
1372
1373
1374
1375
1376
1377
1378
1379
1380
1381
1382
1383
1384
1385
1386
1387
1388
1389
1390
1391
1392
1393
1394
1395
1396
1397
1398
1399
1400
1401
1402
1403
1404
1405
1406
1407
1408
1409
1410
1411
1412
1413
1414
1415
1416
1417
1418
1419
1420
1421
1422
1423
1424
1425
1426
1427
1428
1429
1430
1431
1432
1433
1434
1435
1436
1437
1438
1439
1440
1441
1442
1443
1444
1445
1446
1447
1448
1449
1450
1451
1452
1453
1454
1455
1456
1457
1458
1459
1460
1461
1462
1463
1464
1465
1466
1467
1468
1469
1470
1471
1472
1473
1474
1475
1476
1477
1478
1479
1480
1481
1482
1483
1484
1485
1486
1487
1488
1489
1490
1491
1492
1493
1494
1495
1496
1497
1498
1499
1500
1501
1502
1503
1504
1505
1506
1507
1508
1509
1510
1511
1512
1513
1514
1515
1516
1517
1518
1519
1520
1521
1522
1523
1524
1525
1526
1527
1528
1529
1530
1531
1532
1533
1534
1535
1536
1537
1538
1539
1540
1541
1542
1543
1544
1545
1546
1547
1548
1549
1550
1551
1552
1553
1554
1555
1556
1557
1558
1559
1560
1561
1562
1563
1564
1565
1566
1567
1568
1569
1570
1571
1572
1573
1574
1575
1576
1577
1578
1579
1580
1581
1582
1583
1584
1585
1586
1587
1588
1589
1590
1591
1592
1593
1594
1595
1596
1597
1598
1599
1600
1601
1602
1603
1604
1605
1606
1607
1608
1609
1610
1611
1612
1613
1614
1615
1616
1617
1618
1619
1620
1621
1622
1623
1624
1625
1626
1627
1628
1629
1630
1631
1632
1633
1634
1635
1636
1637
1638
1639
1640
1641
1642
1643
1644
1645
1646
1647
1648
1649
1650
1651
1652
1653
1654
1655
1656
1657
1658
1659
1660
1661
1662
1663
1664
1665
1666
1667
1668
1669
1670
1671
1672
1673
1674
1675
1676
1677
1678
1679
1680
1681
1682
1683
1684
1685
1686
1687
1688
1689
1690
1691
1692
1693
1694
1695
1696
1697
1698
1699
1700
1701
1702
1703
1704
1705
1706
1707
1708
1709
1710
1711
1712
1713
1714
1715
1716
1717
1718
1719
1720
1721
1722
1723
1724
1725
1726
1727
1728
1729
1730
1731
1732
1733
1734
1735
1736
1737
1738
1739
1740
1741
1742
1743
1744
1745
1746
1747
1748
1749
1750
1751
1752
1753
1754
1755
1756
1757
1758
1759
1760
1761
1762
1763
1764
1765
1766
1767
1768
1769
1770
1771
1772
1773
1774
1775
1776
1777
1778
1779
1780
1781
1782
1783
1784
1785
1786
1787
1788
1789
1790
1791
1792
1793
1794
1795
1796
1797
1798
1799
1800
1801
1802
1803
1804
1805
1806
1807
1808
1809
1810
1811
1812
1813
1814
1815
1816
1817
1818
1819
1820
1821
1822
1823
1824
1825
1826
1827
1828
1829
1830
1831
1832
1833
1834
1835
1836
1837
1838
1839
1840
1841
1842
1843
1844
1845
1846
1847
1848
1849
1850
1851
1852
1853
1854
1855
1856
1857
1858
1859
1860
1861
1862
1863
1864
1865
1866
1867
1868
1869
1870
1871
1872
1873
1874
1875
1876
1877
1878
1879
1880
1881
1882
1883
1884
1885
1886
1887
1888
1889
1890
1891
1892
1893
1894
1895
1896
1897
1898
1899
1900
1901
1902
1903
1904
1905
1906
1907
1908
1909
1910
1911
1912
1913
1914
1915
1916
1917
1918
1919
1920
1921
1922
1923
1924
1925
1926
1927
1928
1929
1930
1931
1932
1933
1934
1935
1936
1937
1938
1939
1940
1941
1942
1943
1944
1945
1946
1947
1948
1949
1950
1951
1952
1953
1954
1955
1956
1957
1958
1959
1960
1961
1962
1963
1964
1965
1966
1967
1968
1969
1970
1971
1972
1973
1974
1975
1976
1977
1978
1979
1980
1981
1982
1983
1984
1985
1986
1987
1988
1989
1990
1991
1992
1993
1994
1995
1996
1997
1998
1999
2000
2001
2002
2003
2004
2005
2006
2007
2008
2009
2010
2011
2012
2013
2014
2015
2016
2017
2018
2019
2020
2021
2022
2023
2024
2025
2026
2027
2028
2029
2030
2031
2032
2033
2034
2035
2036
2037
2038
2039
2040
2041
2042
2043
2044
2045
2046
2047
2048
2049
2050
2051
2052
2053
2054
2055
2056
2057
2058
2059
2060
2061
2062
2063
2064
2065
2066
2067
2068
2069
2070
2071
2072
2073
2074
2075
2076
2077
2078
2079
2080
2081
2082
2083
2084
2085
2086
2087
2088
2089
2090
2091
2092
2093
2094
2095
2096
2097
2098
2099
2100
2101
2102
2103
2104
2105
2106
2107
2108
2109
2110
2111
2112
2113
2114
2115
2116
2117
2118
2119
2120
2121
2122
2123
2124
2125
2126
2127
2128
2129
2130
2131
2132
2133
2134
2135
2136
2137
2138
2139
2140
2141
2142
2143
2144
2145
2146
2147
2148
2149
2150
2151
2152
2153
2154
2155
2156
2157
2158
2159
2160
2161
2162
2163
2164
2165
2166
2167
2168
2169
2170
2171
2172
2173
2174
2175
2176
2177
2178
2179
2180
2181
2182
2183
2184
2185
2186
2187
2188
2189
2190
2191
2192
2193
2194
2195
2196
2197
2198
2199
2200
2201
2202
2203
2204
2205
2206
2207
2208
2209
2210
2211
2212
2213
2214
2215
2216
2217
2218
2219
2220
2221
2222
2223
2224
2225
2226
2227
2228
2229
2230
2231
2232
2233
2234
2235
2236
2237
2238
2239
2240
2241
2242
2243
2244
2245
2246
2247
2248
2249
2250
2251
2252
2253
2254
2255
2256
2257
2258
2259
2260
2261
2262
2263
2264
2265
2266
2267
2268
2269
2270
2271
2272
2273
2274
2275
2276
2277
2278
2279
2280
2281
2282
2283
2284
2285
2286
2287
2288
2289
2290
2291
2292
2293
2294
2295
2296
2297
2298
2299
2300
2301
2302
2303
2304
2305
2306
2307
2308
2309
2310
2311
2312
2313
2314
2315
2316
2317
2318
2319
2320
2321
2322
2323
2324
2325
2326
2327
2328
2329
2330
2331
2332
2333
2334
2335
2336
2337
2338
2339
2340
2341
2342
2343
2344
2345
2346
2347
2348
2349
2350
2351
2352
2353
2354
2355
2356
2357
2358
2359
2360
2361
2362
2363
2364
2365
2366
2367
2368
2369
2370
2371
2372
2373
2374
2375
2376
2377
2378
2379
2380
2381
2382
2383
2384
2385
2386
2387
2388
2389
2390
2391
2392
2393
2394
2395
2396
2397
2398
2399
2400
2401
2402
2403
2404
2405
2406
2407
2408
2409
2410
2411
2412
2413
2414
2415
2416
2417
2418
2419
2420
2421
2422
2423
2424
2425
2426
2427
2428
2429
2430
2431
2432
2433
2434
2435
2436
2437
2438
2439
2440
2441
2442
2443
2444
2445
2446
2447
2448
2449
2450
2451
2452
2453
2454
2455
2456
2457
2458
2459
2460
2461
2462
2463
2464
2465
2466
2467
2468
2469
2470
2471
2472
2473
2474
2475
2476
2477
2478
2479
2480
2481
2482
2483
2484
2485
2486
2487
2488
2489
2490
2491
2492
2493
2494
2495
2496
2497
2498
2499
2500
2501
2502
2503
2504
2505
2506
2507
2508
2509
2510
2511
2512
2513
2514
2515
2516
2517
2518
2519
2520
2521
2522
2523
2524
2525
2526
2527
2528
2529
2530
2531
2532
2533
2534
2535
2536
2537
2538
2539
2540
2541
2542
2543
2544
2545
2546
2547
2548
2549
2550
2551
2552
2553
2554
2555
2556
2557
2558
2559
2560
2561
2562
2563
2564
2565
2566
2567
2568
2569
2570
2571
2572
2573
2574
2575
2576
2577
2578
2579
2580
2581
2582
2583
2584
2585
2586
2587
2588
2589
2590
2591
2592
2593
2594
2595
2596
2597
2598
2599
2600
2601
2602
2603
2604
2605
2606
2607
2608
2609
2610
2611
2612
2613
2614
2615
2616
2617
2618
2619
2620
2621
2622
2623
2624
2625
2626
2627
2628
2629
2630
2631
2632
2633
2634
2635
2636
2637
2638
2639
2640
2641
2642
2643
2644
2645
2646
2647
2648
2649
2650
2651
2652
2653
2654
2655
2656
2657
2658
2659
2660
2661
2662
2663
2664
2665
2666
2667
2668
2669
2670
2671
2672
2673
2674
2675
2676
2677
2678
2679
2680
2681
2682
2683
2684
2685
2686
2687
2688
2689
2690
2691
2692
2693
2694
2695
2696
2697
2698
2699
2700
2701
2702
2703
2704
2705
2706
2707
2708
2709
2710
2711
2712
2713
2714
2715
2716
2717
2718
2719
2720
2721
2722
2723
2724
2725
2726
2727
2728
2729
2730
2731
2732
2733
2734
2735
2736
2737
2738
2739
2740
2741
2742
2743
2744
2745
2746
2747
2748
2749
2750
2751
2752
2753
2754
2755
2756
2757
2758
2759
2760
2761
2762
2763
2764
2765
2766
2767
2768
2769
2770
2771
2772
2773
2774
2775
2776
2777
2778
2779
2780
2781
2782
2783
2784
2785
2786
2787
2788
2789
2790
2791
2792
2793
2794
2795
2796
2797
2798
2799
2800
2801
2802
2803
2804
2805
2806
2807
2808
2809
2810
2811
2812
2813
2814
2815
2816
2817
2818
2819
2820
2821
2822
2823
2824
2825
2826
2827
2828
2829
2830
2831
2832
2833
2834
2835
2836
2837
2838
2839
2840
2841
2842
2843
2844
2845
2846
2847
2848
2849
2850
2851
2852
2853
2854
2855
2856
2857
2858
2859
2860
2861
2862
2863
2864
2865
2866
2867
2868
2869
2870
2871
2872
2873
2874
2875
2876
2877
2878
2879
2880
2881
2882
2883
2884
2885
2886
2887
2888
2889
2890
2891
2892
2893
2894
2895
2896
2897
2898
2899
2900
2901
2902
2903
2904
2905
2906
2907
2908
2909
2910
2911
2912
2913
2914
2915
2916
2917
2918
2919
2920
2921
2922
2923
2924
2925
2926
2927
2928
2929
2930
2931
2932
2933
2934
2935
2936
2937
2938
2939
2940
2941
2942
2943
2944
2945
2946
2947
2948
2949
2950
2951
2952
2953
2954
2955
2956
2957
2958
2959
2960
2961
2962
2963
2964
2965
2966
2967
2968
2969
2970
2971
2972
2973
2974
2975
2976
2977
2978
2979
2980
2981
2982
2983
2984
2985
2986
2987
2988
2989
2990
2991
2992
2993
2994
2995
2996
2997
2998
2999
3000
3001
3002
3003
3004
3005
3006
3007
3008
3009
3010
3011
3012
3013
3014
3015
3016
3017
3018
3019
3020
3021
3022
3023
3024
3025
3026
3027
3028
3029
3030
3031
3032
3033
3034
3035
3036
3037
3038
3039
3040
3041
3042
3043
3044
3045
3046
3047
3048
3049
3050
3051
3052
3053
3054
3055
3056
3057
3058
3059
3060
3061
3062
3063
3064
3065
3066
3067
3068
3069
3070
3071
3072
3073
3074
3075
3076
3077
3078
3079
3080
3081
3082
3083
3084
3085
3086
3087
3088
3089
3090
3091
3092
3093
3094
3095
3096
3097
3098
3099
3100
3101
3102
3103
3104
3105
3106
3107
3108
3109
3110
3111
3112
3113
3114
3115
3116
3117
3118
3119
3120
3121
3122
3123
3124
3125
3126
3127
3128
3129
3130
3131
3132
3133
3134
3135
3136
3137
3138
3139
3140
3141
3142
3143
3144
3145
3146
3147
3148
3149
3150
3151
3152
3153
3154
3155
3156
3157
3158
3159
3160
3161
3162
3163
3164
3165
3166
3167
3168
3169
3170
3171
3172
3173
3174
3175
3176
3177
3178
3179
3180
3181
3182
3183
3184
3185
3186
3187
3188
3189
3190
3191
3192
3193
3194
3195
3196
3197
3198
3199
3200
3201
3202
3203
3204
3205
3206
3207
3208
3209
3210
3211
3212
3213
3214
3215
3216
3217
3218
3219
3220
3221
3222
3223
3224
3225
3226
3227
3228
3229
3230
3231
3232
3233
3234
3235
3236
3237
3238
3239
3240
3241
3242
3243
3244
3245
3246
3247
3248
3249
3250
3251
3252
3253
3254
3255
3256
3257
3258
3259
3260
3261
3262
3263
3264
3265
3266
3267
3268
3269
3270
3271
3272
3273
3274
3275
3276
3277
3278
3279
3280
3281
3282
3283
3284
3285
3286
3287
3288
3289
3290
3291
3292
3293
3294
3295
3296
3297
3298
3299
3300
3301
3302
3303
3304
3305
3306
3307
3308
3309
3310
3311
3312
3313
3314
3315
3316
3317
3318
3319
3320
3321
3322
3323
3324
3325
3326
3327
3328
3329
3330
3331
3332
3333
3334
3335
3336
3337
3338
3339
3340
3341
3342
3343
3344
3345
3346
3347
3348
3349
3350
3351
3352
3353
3354
3355
3356
3357
3358
3359
3360
3361
3362
3363
3364
3365
3366
3367
3368
3369
3370
3371
3372
3373
3374
3375
3376
3377
3378
3379
3380
3381
3382
3383
3384
3385
3386
3387
3388
3389
3390
3391
3392
3393
3394
3395
3396
3397
3398
3399
3400
3401
3402
3403
3404
3405
3406
3407
3408
3409
3410
3411
3412
3413
3414
3415
3416
3417
3418
3419
3420
3421
3422
3423
3424
3425
3426
3427
3428
3429
3430
3431
3432
3433
3434
3435
3436
3437
3438
3439
3440
3441
3442
3443
3444
3445
3446
3447
3448
3449
3450
3451
3452
3453
3454
3455
3456
3457
3458
3459
3460
3461
3462
3463
3464
3465
3466
3467
3468
3469
3470
3471
3472
3473
3474
3475
3476
3477
3478
3479
3480
3481
3482
3483
3484
3485
3486
3487
3488
3489
3490
3491
3492
3493
3494
3495
3496
3497
3498
3499
3500
3501
3502
3503
3504
3505
3506
3507
3508
3509
3510
3511
3512
3513
3514
3515
3516
3517
3518
3519
3520
3521
3522
3523
3524
3525
3526
3527
3528
3529
3530
3531
3532
3533
3534
3535
3536
3537
3538
3539
3540
3541
3542
3543
3544
3545
3546
3547
3548
3549
3550
3551
3552
3553
3554
3555
3556
3557
3558
3559
3560
3561
3562
3563
3564
3565
3566
3567
3568
3569
3570
3571
3572
3573
3574
3575
3576
3577
3578
3579
3580
3581
3582
3583
3584
3585
3586
3587
3588
3589
3590
3591
3592
3593
3594
3595
3596
3597
3598
3599
3600
3601
3602
3603
3604
3605
3606
3607
3608
3609
3610
3611
3612
3613
3614
3615
3616
3617
3618
3619
3620
3621
3622
3623
3624
3625
3626
3627
3628
3629
3630
3631
3632
3633
3634
3635
3636
3637
3638
3639
3640
3641
3642
3643
3644
3645
3646
3647
3648
3649
3650
3651
3652
3653
3654
3655
3656
3657
3658
3659
3660
3661
3662
3663
3664
3665
3666
3667
3668
3669
3670
3671
3672
3673
3674
3675
3676
3677
3678
3679
3680
3681
3682
3683
3684
3685
3686
3687
3688
3689
3690
3691
3692
3693
3694
3695
3696
3697
3698
3699
3700
3701
3702
3703
3704
3705
3706
3707
3708
3709
3710
3711
3712
3713
3714
3715
3716
3717
3718
3719
3720
3721
3722
3723
3724
3725
3726
3727
3728
3729
3730
3731
3732
3733
3734
3735
3736
3737
3738
3739
3740
3741
3742
3743
3744
3745
3746
3747
3748
3749
3750
3751
3752
3753
3754
3755
3756
3757
3758
3759
3760
3761
3762
3763
3764
3765
3766
3767
3768
3769
3770
3771
3772
3773
3774
3775
3776
3777
3778
3779
3780
3781
3782
3783
3784
3785
3786
3787
3788
3789
3790
3791
3792
3793
3794
3795
3796
3797
3798
3799
3800
3801
3802
3803
3804
3805
3806
3807
3808
3809
3810
3811
3812
3813
3814
3815
3816
3817
3818
3819
3820
3821
3822
3823
3824
3825
3826
3827
3828
3829
3830
3831
3832
3833
3834
3835
3836
3837
3838
3839
3840
3841
3842
3843
3844
3845
3846
3847
3848
3849
3850
3851
3852
3853
3854
3855
3856
3857
3858
3859
3860
3861
3862
3863
3864
3865
3866
3867
3868
3869
3870
3871
3872
3873
3874
3875
3876
3877
3878
3879
3880
3881
3882
3883
3884
3885
3886
3887
3888
3889
3890
3891
3892
3893
3894
3895
3896
3897
3898
3899
3900
3901
3902
3903
3904
3905
3906
3907
3908
3909
3910
3911
3912
3913
3914
3915
3916
3917
3918
3919
3920
3921
3922
3923
3924
3925
3926
3927
3928
3929
3930
3931
3932
3933
3934
3935
3936
3937
3938
3939
3940
3941
3942
3943
3944
3945
3946
3947
3948
3949
3950
3951
3952
3953
3954
3955
3956
3957
3958
3959
3960
3961
3962
3963
3964
3965
3966
3967
3968
3969
3970
3971
3972
3973
3974
3975
3976
3977
3978
3979
3980
3981
3982
3983
3984
3985
3986
3987
3988
3989
3990
3991
3992
3993
3994
3995
3996
3997
3998
3999
4000
4001
4002
4003
4004
4005
4006
4007
4008
4009
4010
4011
4012
4013
4014
4015
4016
4017
4018
4019
4020
4021
4022
4023
4024
4025
4026
4027
4028
4029
4030
4031
4032
4033
4034
4035
4036
4037
4038
4039
4040
4041
4042
4043
4044
4045
4046
4047
4048
4049
4050
4051
4052
4053
4054
4055
4056
4057
4058
4059
4060
4061
4062
4063
4064
4065
4066
4067
4068
4069
4070
4071
4072
4073
4074
4075
4076
4077
4078
4079
4080
4081
4082
4083
4084
4085
4086
4087
4088
4089
4090
4091
4092
4093
4094
4095
4096
4097
4098
4099
4100
4101
4102
4103
4104
4105
4106
4107
4108
4109
4110
4111
4112
4113
4114
4115
4116
4117
4118
4119
4120
4121
4122
4123
4124
4125
4126
4127
4128
4129
4130
4131
4132
4133
4134
4135
4136
4137
4138
4139
4140
4141
4142
4143
4144
4145
4146
4147
4148
4149
4150
4151
4152
4153
4154
4155
4156
4157
4158
4159
4160
4161
4162
4163
4164
4165
4166
4167
4168
4169
4170
4171
4172
4173
4174
4175
4176
4177
4178
4179
4180
4181
4182
4183
4184
4185
4186
4187
4188
4189
4190
4191
4192
4193
4194
4195
4196
4197
4198
4199
4200
4201
4202
4203
4204
4205
4206
4207
4208
4209
4210
4211
4212
4213
4214
4215
4216
4217
4218
4219
4220
4221
4222
4223
4224
4225
4226
4227
4228
4229
4230
4231
4232
4233
4234
4235
4236
4237
4238
4239
4240
4241
4242
4243
4244
4245
4246
4247
4248
4249
4250
4251
4252
4253
4254
4255
4256
4257
4258
4259
4260
4261
4262
4263
4264
4265
4266
4267
4268
4269
4270
4271
4272
4273
4274
4275
4276
4277
4278
4279
4280
4281
4282
4283
4284
4285
4286
4287
4288
4289
4290
4291
4292
4293
4294
4295
4296
4297
4298
4299
4300
4301
4302
4303
4304
4305
4306
4307
4308
4309
4310
4311
4312
4313
4314
4315
4316
4317
4318
4319
4320
4321
4322
4323
4324
4325
4326
4327
4328
4329
4330
4331
4332
4333
4334
4335
4336
4337
4338
4339
4340
4341
4342
4343
4344
4345
4346
4347
4348
4349
4350
4351
4352
4353
4354
4355
4356
4357
4358
4359
4360
4361
4362
4363
4364
4365
4366
4367
4368
4369
4370
4371
4372
4373
4374
4375
4376
4377
4378
4379
4380
4381
4382
4383
4384
4385
4386
4387
4388
4389
4390
4391
4392
4393
4394
4395
4396
4397
4398
4399
4400
4401
4402
4403
4404
4405
4406
4407
4408
4409
4410
4411
4412
4413
4414
4415
4416
4417
4418
4419
4420
4421
4422
4423
4424
4425
4426
4427
4428
4429
4430
4431
4432
4433
4434
4435
4436
4437
4438
4439
4440
4441
4442
4443
4444
4445
4446
4447
4448
4449
4450
4451
4452
4453
4454
4455
4456
4457
4458
4459
4460
4461
4462
4463
4464
4465
4466
4467
4468
4469
4470
4471
4472
4473
4474
4475
4476
4477
4478
4479
4480
4481
4482
4483
4484
4485
4486
4487
4488
4489
4490
4491
4492
4493
4494
4495
4496
4497
4498
4499
4500
4501
4502
4503
4504
4505
4506
4507
4508
4509
4510
4511
4512
4513
4514
4515
4516
4517
4518
4519
4520
4521
4522
4523
4524
4525
4526
4527
4528
4529
4530
4531
4532
4533
4534
4535
4536
4537
4538
4539
4540
4541
4542
4543
4544
4545
4546
4547
4548
4549
4550
4551
4552
4553
4554
4555
4556
4557
4558
4559
4560
4561
4562
4563
4564
4565
4566
4567
4568
4569
4570
4571
4572
4573
4574
4575
4576
4577
4578
4579
4580
4581
4582
4583
4584
4585
4586
4587
4588
4589
4590
4591
4592
4593
4594
4595
4596
4597
4598
4599
4600
4601
4602
4603
4604
4605
4606
4607
4608
4609
4610
4611
4612
4613
4614
4615
4616
4617
4618
4619
4620
4621
4622
4623
4624
4625
4626
4627
4628
4629
4630
4631
4632
4633
4634
4635
4636
4637
4638
4639
4640
4641
4642
4643
4644
4645
4646
4647
4648
4649
4650
4651
4652
4653
4654
4655
4656
4657
4658
4659
4660
4661
4662
4663
4664
4665
4666
4667
4668
4669
4670
4671
4672
4673
4674
4675
4676
4677
4678
4679
4680
4681
4682
4683
4684
4685
4686
4687
4688
4689
4690
4691
4692
4693
4694
4695
4696
4697
4698
4699
4700
4701
4702
4703
4704
4705
4706
4707
4708
4709
4710
4711
4712
4713
4714
4715
4716
4717
4718
4719
4720
4721
4722
4723
4724
4725
4726
4727
4728
4729
4730
4731
4732
4733
4734
4735
4736
4737
4738
4739
4740
4741
4742
4743
4744
4745
4746
4747
4748
4749
4750
4751
4752
4753
4754
4755
4756
4757
4758
4759
4760
4761
4762
4763
4764
4765
4766
4767
4768
4769
4770
4771
4772
4773
4774
4775
4776
4777
4778
4779
4780
4781
4782
4783
4784
4785
4786
4787
4788
4789
4790
4791
4792
4793
4794
4795
4796
4797
4798
4799
4800
4801
4802
4803
4804
4805
4806
4807
4808
4809
4810
4811
4812
4813
4814
4815
4816
4817
4818
4819
4820
4821
4822
4823
4824
4825
4826
4827
4828
4829
4830
4831
4832
4833
4834
4835
4836
4837
4838
4839
4840
4841
4842
4843
4844
4845
4846
4847
4848
4849
4850
4851
4852
4853
4854
4855
4856
4857
4858
4859
4860
4861
4862
4863
4864
4865
4866
4867
4868
4869
4870
4871
4872
4873
4874
4875
4876
4877
4878
4879
4880
4881
4882
4883
4884
4885
4886
4887
4888
4889
4890
4891
4892
4893
4894
4895
4896
4897
4898
4899
4900
4901
4902
4903
4904
4905
4906
4907
4908
4909
4910
4911
4912
4913
4914
4915
4916
4917
4918
4919
4920
4921
4922
4923
4924
4925
4926
4927
4928
4929
4930
4931
4932
4933
4934
4935
4936
4937
4938
4939
4940
4941
4942
4943
4944
4945
4946
4947
4948
4949
4950
4951
4952
4953
4954
4955
4956
4957
4958
4959
4960
4961
4962
4963
4964
4965
4966
4967
4968
4969
4970
4971
4972
4973
4974
4975
4976
4977
4978
4979
4980
4981
4982
4983
4984
4985
4986
4987
4988
4989
4990
4991
4992
4993
4994
4995
4996
4997
4998
4999
5000
5001
5002
5003
5004
5005
5006
5007
5008
5009
5010
5011
5012
5013
5014
5015
5016
5017
5018
5019
5020
5021
5022
5023
5024
5025
5026
5027
5028
5029
5030
5031
5032
5033
5034
5035
5036
5037
5038
5039
5040
5041
5042
5043
5044
5045
5046
5047
5048
5049
5050
5051
5052
5053
5054
5055
5056
5057
5058
5059
5060
5061
5062
5063
5064
5065
5066
5067
5068
5069
5070
5071
5072
5073
5074
5075
5076
5077
5078
5079
5080
5081
5082
5083
5084
5085
5086
5087
5088
5089
5090
5091
5092
5093
5094
5095
5096
5097
5098
5099
5100
5101
5102
5103
5104
5105
5106
5107
5108
5109
5110
5111
5112
5113
5114
5115
5116
5117
5118
5119
5120
5121
5122
5123
5124
5125
5126
5127
5128
5129
5130
5131
5132
5133
5134
5135
5136
5137
5138
5139
5140
5141
5142
5143
5144
5145
5146
5147
5148
5149
5150
5151
5152
5153
5154
5155
5156
5157
5158
5159
5160
5161
5162
5163
5164
5165
5166
5167
5168
5169
5170
5171
5172
5173
5174
5175
5176
5177
5178
5179
5180
5181
5182
5183
5184
5185
5186
5187
5188
5189
5190
5191
5192
5193
5194
5195
5196
5197
5198
5199
5200
5201
5202
5203
5204
5205
5206
5207
5208
5209
5210
5211
5212
5213
5214
5215
5216
5217
5218
5219
5220
5221
5222
5223
5224
5225
5226
5227
5228
5229
5230
5231
5232
5233
5234
5235
5236
5237
5238
5239
5240
5241
5242
5243
5244
5245
5246
5247
5248
5249
5250
5251
5252
5253
5254
5255
5256
5257
5258
5259
5260
5261
5262
5263
5264
5265
5266
5267
5268
5269
5270
5271
5272
5273
5274
5275
5276
5277
5278
5279
5280
5281
5282
5283
5284
5285
5286
5287
5288
5289
5290
5291
5292
5293
5294
5295
5296
5297
5298
5299
5300
5301
5302
5303
5304
5305
5306
5307
5308
5309
5310
5311
5312
5313
5314
5315
5316
5317
5318
5319
5320
5321
5322
5323
5324
5325
5326
5327
5328
5329
5330
5331
5332
5333
5334
5335
5336
5337
5338
5339
5340
5341
5342
5343
5344
5345
5346
5347
5348
5349
5350
5351
5352
5353
5354
5355
5356
5357
5358
5359
5360
5361
5362
5363
5364
5365
5366
5367
5368
5369
5370
5371
5372
5373
5374
5375
5376
5377
5378
5379
5380
5381
5382
5383
5384
5385
5386
5387
5388
5389
5390
5391
5392
5393
5394
5395
5396
5397
5398
5399
5400
5401
5402
5403
5404
5405
5406
5407
5408
5409
5410
5411
5412
5413
5414
5415
5416
5417
5418
5419
5420
5421
5422
5423
5424
5425
5426
5427
5428
5429
5430
5431
5432
5433
5434
5435
5436
5437
5438
5439
5440
5441
5442
5443
5444
5445
5446
5447
5448
5449
5450
5451
5452
5453
5454
5455
5456
5457
5458
5459
5460
5461
5462
5463
5464
5465
5466
5467
5468
5469
5470
5471
5472
5473
5474
5475
5476
5477
5478
5479
5480
5481
5482
5483
5484
5485
5486
5487
5488
5489
5490
5491
5492
5493
5494
5495
5496
5497
5498
5499
5500
5501
5502
5503
5504
5505
5506
5507
5508
5509
5510
5511
5512
5513
5514
5515
5516
5517
5518
5519
5520
5521
5522
5523
5524
5525
5526
5527
5528
5529
5530
5531
5532
5533
5534
5535
5536
5537
5538
5539
5540
5541
5542
5543
5544
5545
5546
5547
5548
5549
5550
5551
5552
5553
5554
5555
5556
5557
5558
5559
5560
5561
5562
5563
5564
5565
5566
5567
5568
5569
5570
5571
5572
5573
5574
5575
5576
5577
5578
5579
5580
5581
5582
5583
5584
5585
5586
5587
5588
5589
5590
5591
5592
5593
5594
5595
5596
5597
5598
5599
5600
5601
5602
5603
5604
5605
5606
5607
5608
5609
5610
5611
5612
5613
5614
5615
5616
5617
5618
5619
5620
5621
5622
5623
5624
5625
5626
5627
5628
5629
5630
5631
5632
5633
5634
5635
5636
5637
5638
5639
5640
5641
5642
5643
5644
5645
5646
5647
5648
5649
5650
5651
5652
5653
5654
5655
5656
5657
5658
5659
5660
5661
5662
5663
5664
5665
5666
5667
5668
5669
5670
5671
5672
5673
5674
5675
5676
5677
5678
5679
5680
5681
5682
5683
5684
5685
5686
5687
5688
5689
5690
5691
5692
5693
5694
5695
5696
5697
5698
5699
5700
5701
5702
5703
5704
5705
5706
5707
5708
5709
5710
5711
5712
5713
5714
5715
5716
5717
5718
5719
5720
5721
5722
5723
5724
5725
5726
5727
5728
5729
5730
5731
5732
5733
5734
5735
5736
5737
5738
5739
5740
5741
5742
5743
5744
5745
5746
5747
5748
5749
5750
5751
5752
5753
5754
5755
5756
5757
5758
5759
5760
5761
5762
5763
5764
5765
5766
5767
5768
5769
5770
5771
5772
5773
5774
5775
5776
5777
5778
5779
5780
5781
5782
5783
5784
5785
5786
5787
5788
5789
5790
5791
5792
5793
5794
5795
5796
5797
5798
5799
5800
5801
5802
5803
5804
5805
5806
5807
5808
5809
5810
5811
5812
5813
5814
5815
5816
5817
5818
5819
5820
5821
5822
5823
5824
5825
5826
5827
5828
5829
5830
5831
5832
5833
5834
5835
5836
5837
5838
5839
5840
5841
5842
5843
5844
5845
5846
5847
5848
5849
5850
5851
5852
5853
5854
5855
5856
5857
5858
5859
5860
5861
5862
5863
5864
5865
5866
5867
5868
5869
5870
5871
5872
5873
5874
5875
5876
5877
5878
5879
5880
5881
5882
5883
5884
5885
5886
5887
5888
5889
5890
5891
5892
5893
5894
5895
5896
5897
5898
5899
5900
5901
5902
5903
5904
5905
5906
5907
5908
5909
5910
5911
5912
5913
5914
5915
5916
5917
5918
5919
5920
5921
5922
5923
5924
5925
5926
5927
5928
5929
5930
5931
5932
5933
5934
5935
5936
5937
5938
5939
5940
5941
5942
5943
5944
5945
5946
5947
5948
5949
5950
5951
5952
5953
5954
5955
5956
5957
5958
5959
5960
5961
5962
5963
5964
5965
5966
5967
5968
5969
5970
5971
5972
5973
5974
5975
5976
5977
5978
5979
5980
5981
5982
5983
5984
5985
5986
5987
5988
5989
5990
5991
5992
5993
5994
5995
5996
5997
5998
5999
6000
6001
6002
6003
6004
6005
6006
6007
6008
6009
6010
6011
6012
6013
6014
6015
6016
6017
6018
6019
6020
6021
6022
6023
6024
6025
6026
6027
6028
6029
6030
6031
6032
6033
6034
6035
6036
6037
6038
6039
6040
6041
6042
6043
6044
6045
6046
6047
6048
6049
6050
6051
6052
6053
6054
6055
6056
6057
6058
6059
6060
6061
6062
6063
6064
6065
6066
6067
6068
6069
6070
6071
6072
6073
6074
6075
6076
6077
6078
6079
6080
6081
6082
6083
6084
6085
6086
6087
6088
6089
6090
6091
6092
6093
6094
6095
6096
6097
6098
6099
6100
6101
6102
6103
6104
6105
6106
6107
6108
6109
6110
6111
6112
6113
6114
6115
6116
6117
6118
6119
6120
6121
6122
6123
6124
6125
6126
6127
6128
6129
6130
6131
6132
6133
6134
6135
6136
6137
6138
6139
6140
6141
6142
6143
6144
6145
6146
6147
6148
6149
6150
6151
6152
6153
6154
6155
6156
6157
6158
6159
6160
6161
6162
6163
6164
6165
6166
6167
6168
6169
6170
6171
6172
6173
6174
6175
6176
6177
6178
6179
6180
6181
6182
6183
6184
6185
6186
6187
6188
6189
6190
6191
6192
6193
6194
6195
6196
6197
6198
6199
6200
6201
6202
6203
6204
6205
6206
6207
6208
6209
6210
6211
6212
6213
6214
6215
6216
6217
6218
6219
6220
6221
6222
6223
6224
6225
6226
6227
6228
6229
6230
6231
6232
6233
6234
6235
6236
6237
6238
6239
6240
6241
6242
6243
6244
6245
6246
6247
6248
6249
6250
6251
6252
6253
6254
6255
6256
6257
6258
6259
6260
6261
6262
6263
6264
6265
6266
6267
6268
6269
6270
6271
6272
6273
6274
6275
6276
6277
6278
6279
6280
6281
6282
6283
6284
6285
6286
6287
6288
6289
6290
6291
6292
6293
6294
6295
6296
6297
6298
6299
6300
6301
6302
6303
6304
6305
6306
6307
6308
6309
6310
6311
6312
6313
6314
6315
6316
6317
6318
6319
6320
6321
6322
6323
6324
6325
6326
6327
6328
6329
6330
6331
6332
6333
6334
6335
6336
6337
6338
6339
6340
6341
6342
6343
6344
6345
6346
6347
6348
6349
6350
6351
6352
6353
6354
6355
6356
6357
6358
6359
6360
6361
6362
6363
6364
6365
6366
6367
6368
6369
6370
6371
6372
6373
6374
6375
6376
6377
6378
6379
6380
6381
6382
6383
6384
6385
6386
6387
6388
6389
6390
6391
6392
6393
6394
6395
6396
6397
6398
6399
6400
6401
6402
6403
6404
6405
6406
6407
6408
6409
6410
6411
6412
6413
6414
6415
6416
6417
6418
6419
6420
6421
6422
6423
6424
6425
6426
6427
6428
6429
6430
6431
6432
6433
6434
6435
6436
6437
6438
6439
6440
6441
6442
6443
6444
6445
6446
6447
6448
6449
6450
6451
6452
6453
6454
6455
6456
6457
6458
6459
6460
6461
6462
6463
6464
6465
6466
6467
6468
6469
6470
6471
6472
6473
6474
6475
6476
6477
6478
6479
6480
6481
6482
6483
6484
6485
6486
6487
6488
6489
6490
6491
6492
6493
6494
6495
6496
6497
6498
6499
6500
6501
6502
6503
6504
6505
6506
6507
6508
6509
6510
6511
6512
6513
6514
6515
6516
6517
6518
6519
6520
6521
6522
6523
6524
6525
6526
6527
6528
6529
6530
6531
6532
6533
6534
6535
6536
6537
6538
6539
6540
6541
6542
6543
6544
6545
6546
6547
6548
6549
6550
6551
6552
6553
6554
6555
6556
6557
6558
6559
6560
6561
6562
6563
6564
6565
6566
6567
6568
6569
6570
6571
6572
6573
6574
6575
6576
6577
6578
6579
6580
6581
6582
6583
6584
6585
6586
6587
6588
6589
6590
6591
6592
6593
6594
6595
6596
6597
6598
6599
6600
6601
6602
6603
6604
6605
6606
6607
6608
6609
6610
6611
6612
6613
6614
6615
6616
6617
6618
6619
6620
6621
6622
6623
6624
6625
6626
6627
6628
6629
6630
6631
6632
6633
6634
6635
6636
6637
6638
6639
6640
6641
6642
6643
6644
6645
6646
6647
6648
6649
6650
6651
6652
6653
6654
6655
6656
6657
6658
6659
6660
6661
6662
6663
6664
6665
6666
6667
6668
6669
6670
6671
6672
6673
6674
6675
6676
6677
6678
6679
6680
6681
6682
6683
6684
6685
6686
6687
6688
6689
6690
6691
6692
6693
6694
6695
6696
6697
6698
6699
6700
6701
6702
6703
6704
6705
6706
6707
6708
6709
6710
6711
6712
6713
6714
6715
6716
6717
6718
6719
6720
6721
6722
6723
6724
6725
6726
6727
6728
6729
6730
6731
6732
6733
6734
6735
6736
6737
6738
6739
6740
6741
6742
6743
6744
6745
6746
6747
6748
6749
6750
6751
6752
6753
6754
6755
6756
6757
6758
6759
6760
6761
6762
6763
6764
6765
6766
6767
6768
6769
6770
6771
6772
6773
6774
6775
6776
6777
6778
6779
6780
6781
6782
6783
6784
6785
6786
6787
6788
6789
6790
6791
6792
6793
6794
6795
6796
6797
6798
6799
6800
6801
6802
6803
6804
6805
6806
6807
6808
6809
6810
6811
6812
6813
6814
6815
6816
6817
6818
6819
6820
6821
6822
6823
6824
6825
6826
6827
6828
6829
6830
6831
6832
6833
6834
6835
6836
6837
6838
6839
6840
6841
6842
6843
6844
6845
6846
6847
6848
6849
6850
6851
6852
6853
6854
6855
6856
6857
6858
6859
6860
6861
6862
6863
6864
6865
6866
6867
6868
6869
6870
6871
6872
6873
6874
6875
6876
6877
6878
6879
6880
6881
6882
6883
6884
6885
6886
6887
6888
6889
6890
6891
6892
6893
6894
6895
6896
6897
6898
6899
6900
6901
6902
6903
6904
6905
6906
6907
6908
6909
6910
6911
6912
6913
6914
6915
6916
6917
6918
6919
6920
6921
6922
6923
6924
6925
6926
6927
6928
6929
6930
6931
6932
6933
6934
6935
6936
6937
6938
6939
6940
6941
6942
6943
6944
6945
6946
6947
6948
6949
6950
6951
6952
6953
6954
6955
6956
6957
6958
6959
6960
6961
6962
6963
6964
6965
6966
6967
6968
6969
6970
6971
6972
6973
6974
6975
6976
6977
6978
6979
6980
6981
6982
6983
6984
6985
6986
6987
6988
6989
6990
6991
6992
6993
6994
6995
6996
6997
6998
6999
7000
7001
7002
7003
7004
7005
7006
7007
7008
7009
7010
7011
7012
7013
7014
7015
7016
7017
7018
7019
7020
7021
7022
7023
7024
7025
7026
7027
7028
7029
7030
7031
7032
7033
7034
7035
7036
7037
7038
7039
7040
7041
7042
7043
7044
7045
7046
7047
7048
7049
7050
7051
7052
7053
7054
7055
7056
7057
7058
7059
7060
7061
7062
7063
7064
7065
7066
7067
7068
7069
7070
7071
7072
7073
7074
7075
7076
7077
7078
7079
7080
7081
7082
7083
7084
7085
7086
7087
7088
7089
7090
7091
7092
7093
7094
7095
7096
7097
7098
7099
7100
7101
7102
7103
7104
7105
7106
7107
7108
7109
7110
7111
7112
7113
7114
7115
7116
7117
7118
7119
7120
7121
7122
7123
7124
7125
7126
7127
7128
7129
7130
7131
7132
7133
7134
7135
7136
7137
7138
7139
7140
7141
7142
7143
7144
7145
7146
7147
7148
7149
7150
7151
7152
7153
7154
7155
7156
7157
7158
7159
7160
7161
7162
7163
7164
7165
7166
7167
7168
7169
7170
7171
7172
7173
7174
7175
7176
7177
7178
7179
7180
7181
7182
7183
7184
7185
7186
7187
7188
7189
7190
7191
7192
7193
7194
7195
7196
7197
7198
7199
7200
7201
7202
7203
7204
7205
7206
7207
7208
7209
7210
7211
7212
7213
7214
7215
7216
7217
7218
7219
7220
7221
7222
7223
7224
7225
7226
7227
7228
7229
7230
7231
7232
7233
7234
7235
7236
7237
7238
7239
7240
7241
7242
7243
7244
7245
7246
7247
7248
7249
7250
7251
7252
7253
7254
7255
7256
7257
7258
7259
7260
7261
7262
7263
7264
7265
7266
7267
7268
7269
7270
7271
7272
7273
7274
7275
7276
7277
7278
7279
7280
7281
7282
7283
7284
7285
7286
7287
7288
7289
7290
7291
7292
7293
7294
7295
7296
7297
7298
7299
7300
7301
7302
7303
7304
7305
7306
7307
7308
7309
7310
7311
7312
7313
7314
7315
7316
7317
7318
7319
7320
7321
7322
7323
7324
7325
7326
7327
7328
7329
7330
7331
7332
7333
7334
7335
7336
7337
7338
7339
7340
7341
7342
7343
7344
7345
7346
7347
7348
7349
7350
7351
7352
7353
7354
7355
7356
7357
7358
7359
7360
7361
7362
7363
7364
7365
7366
7367
7368
7369
7370
7371
7372
7373
7374
7375
7376
7377
7378
7379
7380
7381
7382
7383
7384
7385
7386
7387
7388
7389
7390
7391
7392
7393
7394
7395
7396
7397
7398
7399
7400
7401
7402
7403
7404
7405
7406
7407
7408
7409
7410
7411
7412
7413
7414
7415
7416
7417
7418
7419
7420
7421
7422
7423
7424
7425
7426
7427
7428
7429
7430
7431
7432
7433
7434
7435
7436
7437
7438
7439
7440
7441
7442
7443
7444
7445
7446
7447
7448
7449
7450
7451
7452
7453
7454
7455
7456
7457
7458
7459
7460
7461
7462
7463
7464
7465
7466
7467
7468
7469
7470
7471
7472
7473
7474
7475
7476
7477
7478
7479
7480
7481
7482
7483
7484
7485
7486
7487
7488
7489
7490
7491
7492
7493
7494
7495
7496
7497
7498
7499
7500
7501
7502
7503
7504
7505
7506
7507
7508
7509
7510
7511
7512
7513
7514
7515
7516
7517
7518
7519
7520
7521
7522
7523
7524
7525
7526
7527
7528
7529
7530
7531
7532
7533
7534
7535
7536
7537
7538
7539
7540
7541
7542
7543
7544
7545
7546
7547
7548
7549
7550
7551
7552
7553
7554
7555
7556
7557
7558
7559
7560
7561
7562
7563
7564
7565
7566
7567
7568
7569
7570
7571
7572
7573
7574
7575
7576
7577
7578
7579
7580
7581
7582
7583
7584
7585
7586
7587
7588
7589
7590
7591
7592
7593
7594
7595
7596
7597
7598
7599
7600
7601
7602
7603
7604
7605
7606
7607
7608
7609
7610
7611
7612
7613
7614
7615
7616
7617
7618
7619
7620
7621
7622
7623
7624
7625
7626
7627
7628
7629
7630
7631
7632
7633
7634
7635
7636
7637
7638
7639
7640
7641
7642
7643
7644
7645
7646
7647
7648
7649
7650
7651
7652
7653
7654
7655
7656
7657
7658
7659
7660
7661
7662
7663
7664
7665
7666
7667
7668
7669
7670
7671
7672
7673
7674
7675
7676
7677
7678
7679
7680
7681
7682
7683
7684
7685
7686
7687
7688
7689
7690
7691
7692
7693
7694
7695
7696
7697
7698
7699
7700
7701
7702
7703
7704
7705
7706
7707
7708
7709
7710
7711
7712
7713
7714
7715
7716
7717
7718
7719
7720
7721
7722
7723
7724
7725
7726
7727
7728
7729
7730
7731
7732
7733
7734
7735
7736
7737
7738
7739
7740
7741
7742
7743
7744
7745
7746
7747
7748
7749
7750
7751
7752
7753
7754
7755
7756
7757
7758
7759
7760
7761
7762
7763
7764
7765
7766
7767
7768
7769
7770
7771
7772
7773
7774
7775
7776
7777
7778
7779
7780
7781
7782
7783
7784
7785
7786
7787
7788
7789
7790
7791
7792
7793
7794
7795
7796
7797
7798
7799
7800
7801
7802
7803
7804
7805
7806
7807
7808
7809
7810
7811
7812
7813
7814
7815
7816
7817
7818
7819
7820
7821
7822
7823
7824
7825
7826
7827
7828
7829
7830
7831
7832
7833
7834
7835
7836
7837
7838
7839
7840
7841
7842
7843
7844
7845
7846
7847
7848
7849
7850
7851
7852
7853
7854
7855
7856
7857
7858
7859
7860
7861
7862
7863
7864
7865
7866
7867
7868
7869
7870
7871
7872
7873
7874
7875
7876
7877
7878
7879
7880
7881
7882
7883
7884
7885
7886
7887
7888
7889
7890
7891
7892
7893
7894
7895
7896
7897
7898
7899
7900
7901
7902
7903
7904
7905
7906
7907
7908
7909
7910
7911
7912
7913
7914
7915
7916
7917
7918
7919
7920
7921
7922
7923
7924
7925
7926
7927
7928
7929
7930
7931
7932
7933
7934
7935
7936
7937
7938
7939
7940
7941
7942
7943
7944
7945
7946
7947
7948
7949
7950
7951
7952
7953
7954
7955
7956
7957
7958
7959
7960
7961
7962
7963
7964
7965
7966
7967
7968
7969
7970
7971
7972
7973
7974
7975
7976
7977
7978
7979
7980
7981
7982
7983
7984
7985
7986
7987
7988
7989
7990
7991
7992
7993
7994
7995
7996
7997
7998
7999
8000
8001
8002
8003
8004
8005
8006
8007
8008
8009
8010
8011
8012
8013
8014
8015
8016
8017
8018
8019
8020
8021
8022
8023
8024
8025
8026
8027
8028
8029
8030
8031
8032
8033
8034
8035
8036
8037
8038
8039
8040
8041
8042
8043
8044
8045
8046
8047
8048
8049
8050
8051
8052
8053
8054
8055
8056
8057
8058
8059
8060
8061
8062
8063
8064
8065
8066
8067
8068
8069
8070
8071
8072
8073
8074
8075
8076
8077
8078
8079
8080
8081
8082
8083
8084
8085
8086
8087
8088
8089
8090
8091
8092
8093
8094
8095
8096
8097
8098
8099
8100
8101
8102
8103
8104
8105
8106
8107
8108
8109
8110
8111
8112
8113
8114
8115
8116
8117
8118
8119
8120
8121
8122
8123
8124
8125
8126
8127
8128
8129
8130
8131
8132
8133
8134
8135
8136
8137
8138
8139
8140
8141
8142
8143
8144
8145
8146
8147
8148
8149
8150
8151
8152
8153
8154
8155
8156
8157
8158
8159
8160
8161
8162
8163
8164
8165
8166
8167
8168
8169
8170
8171
8172
8173
8174
8175
8176
8177
8178
8179
8180
8181
8182
8183
8184
8185
8186
8187
8188
8189
8190
8191
8192
8193
8194
8195
8196
8197
8198
8199
8200
8201
8202
8203
8204
8205
8206
8207
8208
8209
8210
8211
8212
8213
8214
8215
8216
8217
8218
8219
8220
8221
8222
8223
8224
8225
8226
8227
8228
8229
8230
8231
8232
8233
8234
8235
8236
8237
8238
8239
8240
8241
8242
8243
8244
8245
8246
8247
8248
8249
8250
8251
8252
8253
8254
8255
8256
8257
8258
8259
8260
8261
8262
8263
8264
8265
8266
8267
8268
8269
8270
8271
8272
8273
8274
8275
8276
8277
8278
8279
8280
8281
8282
8283
8284
8285
8286
8287
8288
8289
8290
8291
8292
8293
8294
8295
8296
8297
8298
8299
8300
8301
8302
8303
8304
8305
8306
8307
8308
8309
8310
8311
8312
8313
8314
8315
8316
8317
8318
8319
8320
8321
8322
8323
8324
8325
8326
8327
8328
8329
8330
8331
8332
8333
8334
8335
8336
8337
8338
8339
8340
8341
8342
8343
8344
8345
8346
8347
8348
8349
8350
8351
8352
8353
8354
8355
8356
8357
8358
8359
8360
8361
8362
8363
8364
8365
8366
8367
8368
8369
8370
8371
8372
8373
8374
8375
8376
8377
8378
8379
8380
8381
8382
8383
8384
8385
8386
8387
8388
8389
8390
8391
8392
8393
8394
8395
8396
8397
8398
8399
8400
8401
8402
8403
8404
8405
8406
8407
8408
8409
8410
8411
8412
8413
8414
8415
8416
8417
8418
8419
8420
8421
8422
8423
8424
8425
8426
8427
8428
8429
8430
8431
8432
8433
8434
8435
8436
8437
8438
8439
8440
8441
8442
8443
8444
8445
8446
8447
8448
8449
8450
8451
8452
8453
8454
8455
8456
8457
8458
8459
8460
8461
8462
8463
8464
8465
8466
8467
8468
8469
8470
8471
8472
8473
8474
8475
8476
8477
8478
8479
8480
8481
8482
8483
8484
8485
8486
8487
8488
8489
8490
8491
8492
8493
8494
8495
8496
8497
8498
8499
8500
8501
8502
8503
8504
8505
8506
8507
8508
8509
8510
8511
8512
8513
8514
8515
8516
8517
8518
8519
8520
8521
8522
8523
8524
8525
8526
8527
8528
8529
8530
8531
8532
8533
8534
8535
8536
8537
8538
8539
8540
8541
8542
8543
8544
8545
8546
8547
8548
8549
8550
8551
8552
8553
8554
8555
8556
8557
8558
8559
8560
8561
8562
8563
8564
8565
8566
8567
8568
8569
8570
8571
8572
8573
8574
8575
8576
8577
8578
8579
8580
8581
8582
8583
8584
8585
8586
8587
8588
8589
8590
8591
8592
8593
8594
8595
8596
8597
8598
8599
8600
8601
8602
8603
8604
8605
8606
8607
8608
8609
8610
8611
8612
8613
8614
8615
8616
8617
8618
8619
8620
8621
8622
8623
8624
8625
8626
8627
8628
8629
8630
8631
8632
8633
8634
8635
8636
8637
8638
8639
8640
8641
8642
8643
8644
8645
8646
8647
8648
8649
8650
8651
8652
8653
8654
8655
8656
8657
8658
8659
8660
8661
8662
8663
8664
8665
8666
8667
8668
8669
8670
8671
8672
8673
8674
8675
8676
8677
8678
8679
8680
8681
8682
8683
8684
8685
8686
8687
8688
8689
8690
8691
8692
8693
8694
8695
8696
8697
8698
8699
8700
8701
8702
8703
8704
8705
8706
8707
8708
8709
8710
8711
8712
8713
8714
8715
8716
8717
8718
8719
8720
8721
8722
8723
8724
8725
8726
8727
8728
8729
8730
8731
8732
8733
8734
8735
8736
8737
8738
8739
8740
8741
8742
8743
8744
8745
8746
8747
8748
8749
8750
8751
8752
8753
8754
8755
8756
8757
8758
8759
8760
8761
8762
8763
8764
8765
8766
8767
8768
8769
8770
8771
8772
8773
8774
8775
8776
8777
8778
8779
8780
8781
8782
8783
8784
8785
8786
8787
8788
8789
8790
8791
8792
8793
8794
8795
8796
8797
8798
8799
8800
8801
8802
8803
8804
8805
8806
8807
8808
8809
8810
8811
8812
8813
8814
8815
8816
8817
8818
8819
8820
8821
8822
8823
8824
8825
8826
8827
8828
8829
8830
8831
8832
8833
8834
8835
8836
8837
8838
8839
8840
8841
8842
8843
8844
8845
8846
8847
8848
8849
8850
8851
8852
8853
8854
8855
8856
8857
8858
8859
8860
8861
8862
8863
8864
8865
8866
8867
8868
8869
8870
8871
8872
8873
8874
8875
8876
8877
8878
8879
8880
8881
8882
8883
8884
8885
8886
8887
8888
8889
8890
8891
8892
8893
8894
8895
8896
8897
8898
8899
8900
8901
8902
8903
8904
8905
8906
8907
8908
8909
8910
8911
8912
8913
8914
8915
8916
8917
8918
8919
8920
8921
8922
8923
8924
8925
8926
8927
8928
8929
8930
8931
8932
8933
8934
8935
8936
8937
8938
8939
8940
8941
8942
8943
8944
8945
8946
8947
8948
8949
8950
8951
8952
8953
8954
8955
8956
8957
8958
8959
8960
8961
8962
8963
8964
8965
8966
8967
8968
8969
8970
8971
8972
8973
8974
8975
8976
8977
8978
8979
8980
8981
8982
8983
8984
8985
8986
8987
8988
8989
8990
8991
8992
8993
8994
8995
8996
8997
8998
8999
9000
9001
9002
9003
9004
9005
9006
9007
9008
9009
9010
9011
9012
9013
9014
9015
9016
9017
9018
9019
9020
9021
9022
9023
9024
9025
9026
9027
9028
9029
9030
9031
9032
9033
9034
9035
9036
9037
9038
9039
9040
9041
9042
9043
9044
9045
9046
9047
9048
9049
9050
9051
9052
9053
9054
9055
9056
9057
9058
9059
9060
9061
9062
9063
9064
9065
9066
9067
9068
9069
9070
9071
9072
9073
9074
9075
9076
9077
9078
9079
9080
9081
9082
9083
9084
9085
9086
9087
9088
9089
9090
9091
9092
9093
9094
9095
9096
9097
9098
9099
9100
9101
9102
9103
9104
9105
9106
9107
9108
9109
9110
9111
9112
9113
9114
9115
9116
9117
9118
9119
9120
9121
9122
9123
9124
9125
9126
9127
9128
9129
9130
9131
9132
9133
9134
9135
9136
9137
9138
9139
9140
9141
9142
9143
9144
9145
9146
9147
9148
9149
9150
9151
9152
9153
9154
9155
9156
9157
9158
9159
9160
9161
9162
9163
9164
9165
9166
9167
9168
9169
9170
9171
9172
9173
9174
9175
9176
9177
9178
9179
9180
9181
9182
9183
9184
9185
9186
9187
9188
9189
9190
9191
9192
9193
9194
9195
9196
9197
9198
9199
9200
9201
9202
9203
9204
9205
9206
9207
9208
9209
9210
9211
9212
9213
9214
9215
9216
9217
9218
9219
9220
9221
9222
9223
9224
9225
9226
9227
9228
9229
9230
9231
9232
9233
9234
9235
9236
9237
9238
9239
9240
9241
9242
9243
9244
9245
9246
9247
9248
9249
9250
9251
9252
9253
9254
9255
9256
9257
9258
9259
9260
9261
9262
9263
9264
9265
9266
9267
9268
9269
9270
9271
9272
9273
9274
9275
9276
9277
9278
9279
9280
9281
9282
9283
9284
9285
9286
9287
9288
9289
9290
9291
9292
9293
9294
9295
9296
9297
9298
9299
9300
9301
9302
9303
9304
9305
9306
9307
9308
9309
9310
9311
9312
9313
9314
9315
9316
9317
9318
9319
9320
9321
9322
9323
9324
9325
9326
9327
9328
9329
9330
9331
9332
9333
9334
9335
9336
9337
9338
9339
9340
9341
9342
9343
9344
9345
9346
9347
9348
9349
9350
9351
9352
9353
9354
9355
9356
9357
9358
9359
9360
9361
9362
9363
9364
9365
9366
9367
9368
9369
9370
9371
9372
9373
9374
9375
9376
9377
9378
9379
9380
9381
9382
9383
9384
9385
9386
9387
9388
9389
9390
9391
9392
9393
9394
9395
9396
9397
9398
9399
9400
9401
9402
9403
9404
9405
9406
9407
9408
9409
9410
9411
9412
9413
9414
9415
9416
9417
9418
9419
9420
9421
9422
9423
9424
9425
9426
9427
9428
9429
9430
9431
9432
9433
9434
9435
9436
9437
9438
9439
9440
9441
9442
9443
9444
9445
9446
9447
9448
9449
9450
9451
9452
9453
9454
9455
9456
9457
9458
9459
9460
9461
9462
9463
9464
9465
9466
9467
9468
9469
9470
9471
9472
9473
9474
9475
9476
9477
9478
9479
9480
9481
9482
9483
9484
9485
9486
9487
9488
9489
9490
9491
9492
9493
9494
9495
9496
9497
9498
9499
9500
9501
9502
9503
9504
9505
9506
9507
9508
9509
9510
9511
9512
9513
9514
9515
9516
9517
9518
9519
9520
9521
9522
9523
9524
9525
9526
9527
9528
9529
9530
9531
9532
9533
9534
9535
9536
9537
9538
9539
9540
9541
9542
9543
9544
9545
9546
9547
9548
9549
9550
9551
9552
9553
9554
9555
9556
9557
9558
9559
9560
9561
9562
9563
9564
9565
9566
9567
9568
9569
9570
9571
9572
9573
9574
9575
9576
9577
9578
9579
9580
9581
9582
9583
9584
9585
9586
9587
9588
9589
9590
9591
9592
9593
9594
9595
9596
9597
9598
9599
9600
9601
9602
9603
9604
9605
9606
9607
9608
9609
9610
9611
9612
9613
9614
9615
9616
9617
9618
9619
9620
9621
9622
9623
9624
9625
9626
9627
9628
9629
9630
9631
9632
9633
9634
9635
9636
9637
9638
9639
9640
9641
9642
9643
9644
9645
9646
9647
9648
9649
9650
9651
9652
9653
9654
9655
9656
9657
9658
9659
9660
9661
9662
9663
9664
9665
9666
9667
9668
9669
9670
9671
9672
9673
9674
9675
9676
9677
9678
9679
9680
9681
9682
9683
9684
9685
9686
9687
9688
9689
9690
9691
9692
9693
9694
9695
9696
9697
9698
9699
9700
9701
9702
9703
9704
9705
9706
9707
9708
9709
9710
9711
9712
9713
9714
9715
9716
9717
9718
9719
9720
9721
9722
9723
9724
9725
9726
9727
9728
9729
9730
9731
9732
9733
9734
9735
9736
9737
9738
9739
9740
9741
9742
9743
9744
9745
9746
9747
9748
9749
9750
9751
9752
9753
9754
9755
9756
9757
9758
9759
9760
9761
9762
9763
9764
9765
9766
9767
9768
9769
9770
9771
9772
9773
9774
9775
9776
9777
9778
9779
9780
9781
9782
9783
9784
9785
9786
9787
9788
9789
9790
9791
9792
9793
9794
9795
9796
9797
9798
9799
9800
9801
9802
9803
9804
9805
9806
9807
9808
9809
9810
9811
9812
9813
9814
9815
9816
9817
9818
9819
9820
9821
9822
9823
9824
9825
9826
9827
9828
9829
9830
9831
9832
9833
9834
9835
9836
9837
9838
9839
9840
9841
9842
9843
9844
9845
9846
9847
9848
9849
9850
9851
9852
9853
9854
9855
9856
9857
9858
9859
9860
9861
9862
9863
9864
9865
9866
9867
9868
9869
9870
9871
9872
9873
9874
9875
9876
9877
9878
9879
9880
9881
9882
9883
9884
9885
9886
9887
9888
9889
9890
9891
9892
9893
9894
9895
9896
9897
9898
9899
9900
9901
9902
9903
9904
9905
9906
9907
9908
9909
9910
9911
9912
9913
9914
9915
9916
9917
9918
9919
9920
9921
9922
9923
9924
9925
9926
9927
9928
9929
9930
9931
9932
9933
9934
9935
9936
9937
9938
9939
9940
9941
9942
9943
9944
9945
9946
9947
9948
9949
9950
9951
9952
9953
9954
9955
9956
9957
9958
9959
9960
9961
9962
9963
9964
9965
9966
9967
9968
9969
9970
9971
9972
9973
9974
9975
9976
9977
9978
9979
9980
9981
9982
9983
9984
9985
9986
9987
9988
9989
9990
9991
9992
9993
9994
9995
9996
9997
9998
9999
10000
10001
10002
10003
10004
10005
10006
10007
10008
10009
10010
10011
10012
10013
10014
10015
10016
10017
10018
10019
10020
10021
10022
10023
10024
10025
10026
10027
10028
10029
10030
10031
10032
10033
10034
10035
10036
10037
10038
10039
10040
10041
10042
10043
10044
10045
10046
10047
10048
10049
10050
10051
10052
10053
10054
10055
10056
10057
10058
10059
10060
10061
10062
10063
10064
10065
10066
10067
10068
10069
10070
10071
10072
10073
10074
10075
10076
10077
10078
10079
10080
10081
10082
10083
10084
10085
10086
10087
10088
10089
10090
10091
10092
10093
10094
10095
10096
10097
10098
10099
10100
10101
10102
10103
10104
10105
10106
10107
10108
10109
10110
10111
10112
10113
10114
10115
10116
10117
10118
10119
10120
10121
10122
10123
10124
10125
10126
10127
10128
10129
10130
10131
10132
10133
10134
10135
10136
10137
10138
10139
10140
10141
10142
10143
10144
10145
10146
10147
10148
10149
10150
10151
10152
10153
10154
10155
10156
10157
10158
10159
10160
10161
10162
10163
10164
10165
10166
10167
10168
10169
10170
10171
10172
10173
10174
10175
10176
10177
10178
10179
10180
10181
10182
10183
10184
10185
10186
10187
10188
10189
10190
10191
10192
10193
10194
10195
10196
10197
10198
10199
10200
10201
10202
10203
10204
10205
10206
10207
10208
10209
10210
10211
10212
10213
10214
10215
10216
10217
10218
10219
10220
10221
10222
10223
10224
10225
10226
10227
10228
10229
10230
10231
10232
10233
10234
10235
10236
10237
10238
10239
10240
10241
10242
10243
10244
10245
10246
10247
10248
10249
10250
10251
10252
10253
10254
10255
10256
10257
10258
10259
10260
10261
10262
10263
10264
10265
10266
10267
10268
10269
10270
10271
10272
10273
10274
10275
10276
10277
10278
10279
10280
10281
10282
10283
10284
10285
10286
10287
10288
10289
10290
10291
10292
10293
10294
10295
10296
10297
10298
10299
10300
10301
10302
10303
10304
10305
10306
10307
10308
10309
10310
10311
10312
10313
10314
10315
10316
10317
10318
10319
10320
10321
10322
10323
10324
10325
10326
10327
10328
10329
10330
10331
10332
10333
10334
10335
10336
10337
10338
10339
10340
10341
10342
10343
10344
10345
10346
10347
10348
10349
10350
10351
10352
10353
10354
10355
10356
10357
10358
10359
10360
10361
10362
10363
10364
10365
10366
10367
10368
10369
10370
10371
10372
10373
10374
10375
10376
10377
10378
10379
10380
10381
10382
10383
10384
10385
10386
10387
10388
10389
10390
10391
10392
10393
10394
10395
10396
10397
10398
10399
10400
10401
10402
10403
10404
10405
10406
10407
10408
10409
10410
10411
10412
10413
10414
10415
10416
10417
10418
10419
10420
10421
10422
10423
10424
10425
10426
10427
10428
10429
10430
10431
10432
10433
10434
10435
10436
10437
10438
10439
10440
10441
10442
10443
10444
10445
10446
10447
10448
10449
10450
10451
10452
10453
10454
10455
10456
10457
10458
10459
10460
10461
10462
10463
10464
10465
10466
10467
10468
10469
10470
10471
10472
10473
10474
10475
10476
10477
10478
10479
10480
10481
10482
10483
10484
10485
10486
10487
10488
10489
10490
10491
10492
10493
10494
10495
10496
10497
10498
10499
10500
10501
10502
10503
10504
10505
10506
10507
10508
10509
10510
10511
10512
10513
10514
10515
10516
10517
10518
10519
10520
10521
10522
10523
10524
10525
10526
10527
10528
10529
10530
10531
10532
10533
10534
10535
10536
10537
10538
10539
10540
10541
10542
10543
10544
10545
10546
10547
10548
10549
10550
10551
10552
10553
10554
10555
10556
10557
10558
10559
10560
10561
10562
10563
10564
10565
10566
10567
10568
10569
10570
10571
10572
10573
10574
10575
10576
10577
10578
10579
10580
10581
10582
10583
10584
10585
10586
10587
10588
10589
10590
10591
10592
10593
10594
10595
10596
10597
10598
10599
10600
10601
10602
10603
10604
10605
10606
10607
10608
10609
10610
10611
10612
10613
10614
10615
10616
10617
10618
10619
10620
10621
10622
10623
10624
10625
10626
10627
10628
10629
10630
10631
10632
10633
10634
10635
10636
10637
10638
10639
10640
10641
10642
10643
10644
10645
10646
10647
10648
10649
10650
10651
10652
10653
10654
10655
10656
10657
10658
10659
10660
10661
10662
10663
10664
10665
10666
10667
10668
10669
10670
10671
10672
10673
10674
10675
10676
10677
10678
10679
10680
10681
10682
10683
10684
10685
10686
10687
10688
10689
10690
10691
10692
10693
10694
10695
10696
10697
10698
10699
10700
10701
10702
10703
10704
10705
10706
10707
10708
10709
10710
10711
10712
10713
10714
10715
10716
10717
10718
10719
10720
10721
10722
10723
10724
10725
10726
10727
10728
10729
10730
10731
10732
10733
10734
10735
10736
10737
10738
10739
10740
10741
10742
10743
10744
10745
10746
10747
10748
10749
10750
10751
10752
10753
10754
10755
10756
10757
10758
10759
10760
10761
10762
10763
10764
10765
10766
10767
10768
10769
10770
10771
10772
10773
10774
10775
10776
10777
10778
10779
10780
10781
10782
10783
10784
10785
10786
10787
10788
10789
10790
10791
10792
10793
10794
10795
10796
10797
10798
10799
10800
10801
10802
10803
10804
10805
10806
10807
10808
10809
10810
10811
10812
10813
10814
10815
10816
10817
10818
10819
10820
10821
10822
10823
10824
10825
10826
10827
10828
10829
10830
10831
10832
10833
10834
10835
10836
10837
10838
10839
10840
10841
10842
10843
10844
10845
10846
10847
10848
10849
10850
10851
10852
10853
10854
10855
10856
10857
10858
10859
10860
10861
10862
10863
10864
10865
10866
10867
10868
10869
10870
10871
10872
10873
10874
10875
10876
10877
10878
10879
10880
10881
10882
10883
10884
10885
10886
10887
10888
10889
10890
10891
10892
10893
10894
10895
10896
10897
10898
10899
10900
10901
10902
10903
10904
10905
10906
10907
10908
10909
10910
10911
10912
10913
10914
10915
10916
10917
10918
10919
10920
10921
10922
10923
10924
10925
10926
10927
10928
10929
10930
10931
10932
10933
10934
10935
10936
10937
10938
10939
10940
10941
10942
10943
10944
10945
10946
10947
10948
10949
10950
10951
10952
10953
10954
10955
10956
10957
10958
10959
10960
10961
10962
10963
10964
10965
10966
10967
10968
10969
10970
10971
10972
10973
10974
10975
10976
10977
10978
10979
10980
10981
10982
10983
10984
10985
10986
10987
10988
10989
10990
10991
10992
10993
10994
10995
10996
10997
10998
10999
11000
11001
11002
11003
11004
11005
11006
11007
11008
11009
11010
11011
11012
11013
11014
11015
11016
11017
11018
11019
11020
11021
11022
11023
11024
11025
11026
11027
11028
11029
11030
11031
11032
11033
11034
11035
11036
11037
11038
11039
11040
11041
11042
11043
11044
11045
11046
11047
11048
11049
11050
11051
11052
11053
11054
11055
11056
11057
11058
11059
11060
11061
11062
11063
11064
11065
11066
11067
11068
11069
11070
11071
11072
11073
11074
11075
11076
11077
11078
11079
11080
11081
11082
11083
11084
11085
11086
11087
11088
11089
11090
11091
11092
11093
11094
11095
11096
11097
11098
11099
11100
11101
11102
11103
11104
11105
11106
11107
11108
11109
11110
11111
11112
11113
11114
11115
11116
11117
11118
11119
11120
11121
11122
11123
11124
11125
11126
11127
11128
11129
11130
11131
11132
11133
11134
11135
11136
11137
11138
11139
11140
11141
11142
11143
11144
11145
11146
11147
11148
11149
11150
11151
11152
11153
11154
11155
11156
11157
11158
11159
11160
11161
11162
11163
11164
11165
11166
11167
11168
11169
11170
11171
11172
11173
11174
11175
11176
11177
11178
11179
11180
11181
11182
11183
11184
11185
11186
11187
11188
11189
11190
11191
11192
11193
11194
11195
11196
11197
11198
11199
11200
11201
11202
11203
11204
11205
11206
11207
11208
11209
11210
11211
11212
11213
11214
11215
11216
11217
11218
11219
11220
11221
11222
11223
11224
11225
11226
11227
11228
11229
11230
11231
11232
11233
11234
11235
11236
11237
11238
11239
11240
11241
11242
11243
11244
11245
11246
11247
11248
11249
11250
11251
11252
11253
11254
11255
11256
11257
11258
11259
11260
11261
11262
11263
11264
11265
11266
11267
11268
11269
11270
11271
11272
11273
11274
11275
11276
11277
11278
11279
11280
11281
11282
11283
11284
11285
11286
11287
11288
11289
11290
11291
11292
11293
11294
11295
11296
11297
11298
11299
11300
11301
11302
11303
11304
11305
11306
11307
11308
11309
11310
11311
11312
11313
11314
11315
11316
11317
11318
11319
11320
11321
11322
11323
11324
11325
11326
11327
11328
11329
11330
11331
11332
11333
11334
11335
11336
11337
11338
11339
11340
11341
11342
11343
11344
11345
11346
11347
11348
11349
11350
11351
11352
11353
11354
11355
11356
11357
11358
11359
11360
11361
11362
11363
11364
11365
11366
11367
11368
11369
11370
11371
11372
11373
11374
11375
11376
11377
11378
11379
11380
11381
11382
11383
11384
11385
11386
11387
11388
11389
11390
11391
11392
11393
11394
11395
11396
11397
11398
11399
11400
11401
11402
11403
11404
11405
11406
11407
11408
11409
11410
11411
11412
11413
11414
11415
11416
11417
11418
11419
11420
11421
11422
11423
11424
11425
11426
11427
11428
11429
11430
11431
11432
11433
11434
11435
11436
11437
11438
11439
11440
11441
11442
11443
11444
11445
11446
11447
11448
11449
11450
11451
11452
11453
11454
11455
11456
11457
11458
11459
11460
11461
11462
11463
11464
11465
11466
11467
11468
11469
11470
11471
11472
11473
11474
11475
11476
11477
11478
11479
11480
11481
11482
11483
11484
11485
11486
11487
11488
11489
11490
11491
11492
11493
11494
11495
11496
11497
11498
11499
11500
11501
11502
11503
11504
11505
11506
11507
11508
11509
11510
11511
11512
11513
11514
11515
11516
11517
11518
11519
11520
11521
11522
11523
11524
11525
11526
11527
11528
11529
11530
11531
11532
11533
11534
11535
11536
11537
11538
11539
11540
11541
11542
11543
11544
11545
11546
11547
11548
11549
11550
11551
11552
11553
11554
11555
11556
11557
11558
11559
11560
11561
11562
11563
11564
11565
11566
11567
11568
11569
11570
11571
11572
11573
11574
11575
11576
11577
11578
11579
11580
11581
11582
11583
11584
11585
11586
11587
11588
11589
11590
11591
11592
11593
11594
11595
11596
11597
11598
11599
11600
11601
11602
11603
11604
11605
11606
11607
11608
11609
11610
11611
11612
11613
11614
11615
11616
11617
11618
11619
11620
11621
11622
11623
11624
11625
11626
11627
11628
11629
11630
11631
11632
11633
11634
11635
11636
11637
11638
11639
11640
11641
11642
11643
11644
11645
11646
11647
11648
11649
11650
11651
11652
11653
11654
11655
11656
11657
11658
11659
11660
11661
11662
11663
11664
11665
11666
11667
11668
11669
11670
11671
11672
11673
11674
11675
11676
11677
11678
11679
11680
11681
11682
11683
11684
11685
11686
11687
11688
11689
11690
11691
11692
11693
11694
11695
11696
11697
11698
11699
11700
11701
11702
11703
11704
11705
11706
11707
11708
11709
11710
11711
11712
11713
11714
11715
11716
11717
11718
11719
11720
11721
11722
11723
11724
11725
11726
11727
11728
11729
11730
11731
11732
11733
11734
11735
11736
11737
11738
11739
11740
11741
11742
11743
11744
11745
11746
11747
11748
11749
11750
11751
11752
11753
11754
11755
11756
11757
11758
11759
11760
11761
11762
11763
11764
11765
11766
11767
11768
11769
11770
11771
11772
11773
11774
11775
11776
11777
11778
11779
11780
11781
11782
11783
11784
11785
11786
11787
11788
11789
11790
11791
11792
11793
11794
11795
11796
11797
11798
11799
11800
11801
11802
11803
11804
11805
11806
11807
11808
11809
11810
11811
11812
11813
11814
11815
11816
11817
11818
11819
11820
11821
11822
11823
11824
11825
11826
11827
11828
11829
11830
11831
11832
11833
11834
11835
11836
11837
11838
11839
11840
11841
11842
11843
11844
11845
11846
11847
11848
11849
11850
11851
11852
11853
11854
11855
11856
11857
11858
11859
11860
11861
11862
11863
11864
11865
11866
11867
11868
11869
11870
11871
11872
11873
11874
11875
11876
11877
11878
11879
11880
11881
11882
11883
11884
11885
11886
11887
11888
11889
11890
11891
11892
11893
11894
11895
11896
11897
11898
11899
11900
11901
11902
11903
11904
11905
11906
11907
11908
11909
11910
11911
11912
11913
11914
11915
11916
11917
11918
11919
11920
11921
11922
11923
11924
11925
11926
11927
11928
11929
11930
11931
11932
11933
11934
11935
11936
11937
11938
11939
11940
11941
11942
11943
11944
11945
11946
11947
11948
11949
11950
11951
11952
11953
11954
11955
11956
11957
11958
11959
11960
11961
11962
11963
11964
11965
11966
11967
11968
11969
11970
11971
11972
11973
11974
11975
11976
11977
11978
11979
11980
11981
11982
11983
11984
11985
11986
11987
11988
11989
11990
11991
11992
11993
11994
11995
11996
11997
11998
11999
12000
12001
12002
12003
12004
12005
12006
12007
12008
12009
12010
12011
12012
12013
12014
12015
12016
12017
12018
12019
12020
12021
12022
12023
12024
12025
12026
12027
12028
12029
12030
12031
12032
12033
12034
12035
12036
12037
12038
12039
12040
12041
12042
12043
12044
12045
12046
12047
12048
12049
12050
12051
12052
12053
12054
12055
12056
12057
12058
12059
12060
12061
12062
12063
12064
12065
12066
12067
12068
12069
12070
12071
12072
12073
12074
12075
12076
12077
12078
12079
12080
12081
12082
12083
12084
12085
12086
12087
12088
12089
12090
12091
12092
12093
12094
12095
12096
12097
12098
12099
12100
12101
12102
12103
12104
12105
12106
12107
12108
12109
12110
12111
12112
12113
12114
12115
12116
12117
12118
12119
12120
12121
12122
12123
12124
12125
12126
12127
12128
12129
12130
12131
12132
12133
12134
12135
12136
12137
12138
12139
12140
12141
12142
12143
12144
12145
12146
12147
12148
12149
12150
12151
12152
12153
12154
12155
12156
12157
12158
12159
12160
12161
12162
12163
12164
12165
12166
12167
12168
12169
12170
12171
12172
12173
12174
12175
12176
12177
12178
12179
12180
12181
12182
12183
12184
12185
12186
12187
12188
12189
12190
12191
12192
12193
12194
12195
12196
12197
12198
12199
12200
12201
12202
12203
12204
12205
12206
12207
12208
12209
12210
12211
12212
12213
12214
12215
12216
12217
12218
12219
12220
12221
12222
12223
12224
12225
12226
12227
12228
12229
12230
12231
12232
12233
12234
12235
12236
12237
12238
12239
12240
12241
12242
12243
12244
12245
12246
12247
12248
12249
12250
12251
12252
12253
12254
12255
12256
12257
12258
12259
12260
12261
12262
12263
12264
12265
12266
12267
12268
12269
12270
12271
12272
12273
12274
12275
12276
12277
12278
12279
12280
12281
12282
12283
12284
12285
12286
12287
12288
12289
12290
12291
12292
12293
12294
12295
12296
12297
12298
12299
12300
12301
12302
12303
12304
12305
12306
12307
12308
12309
12310
12311
12312
12313
12314
12315
12316
12317
12318
12319
12320
12321
12322
12323
12324
12325
12326
12327
12328
12329
12330
12331
12332
12333
12334
12335
12336
12337
12338
12339
12340
12341
12342
12343
12344
12345
12346
12347
12348
12349
12350
12351
12352
12353
12354
12355
12356
12357
12358
12359
12360
12361
12362
12363
12364
12365
12366
12367
12368
12369
12370
12371
12372
12373
12374
12375
12376
12377
12378
12379
12380
12381
12382
12383
12384
12385
12386
12387
12388
12389
12390
12391
12392
12393
12394
12395
12396
12397
12398
12399
12400
12401
12402
12403
12404
12405
12406
12407
12408
12409
12410
12411
12412
12413
12414
12415
12416
12417
12418
12419
12420
12421
12422
12423
12424
12425
12426
12427
12428
12429
12430
12431
12432
12433
12434
12435
12436
12437
12438
12439
12440
12441
12442
12443
12444
12445
12446
12447
12448
12449
12450
12451
12452
12453
12454
12455
12456
12457
12458
12459
12460
12461
12462
12463
12464
12465
12466
12467
12468
12469
12470
12471
12472
12473
12474
12475
12476
12477
12478
12479
12480
12481
12482
12483
12484
12485
12486
12487
12488
12489
12490
12491
12492
12493
12494
12495
12496
12497
12498
12499
12500
12501
12502
12503
12504
12505
12506
12507
12508
12509
12510
12511
12512
12513
12514
12515
12516
12517
12518
12519
12520
12521
12522
12523
12524
12525
12526
12527
12528
12529
12530
12531
12532
12533
12534
12535
12536
12537
12538
12539
12540
12541
12542
12543
12544
12545
12546
12547
12548
12549
12550
12551
12552
12553
12554
12555
12556
12557
12558
12559
12560
12561
12562
12563
12564
12565
12566
12567
12568
12569
12570
12571
12572
12573
12574
12575
12576
12577
12578
12579
12580
12581
12582
12583
12584
12585
12586
12587
12588
12589
12590
12591
12592
12593
12594
12595
12596
12597
12598
12599
12600
12601
12602
12603
12604
12605
12606
12607
12608
12609
12610
12611
12612
12613
12614
12615
12616
12617
12618
12619
12620
12621
12622
12623
12624
12625
12626
12627
12628
12629
12630
12631
12632
12633
12634
12635
12636
12637
12638
12639
12640
12641
12642
12643
12644
12645
12646
12647
12648
12649
12650
12651
12652
12653
12654
12655
12656
12657
12658
12659
12660
12661
12662
12663
12664
12665
12666
12667
12668
12669
12670
12671
12672
12673
12674
12675
12676
12677
12678
12679
12680
12681
12682
12683
12684
12685
12686
12687
12688
12689
12690
12691
12692
12693
12694
12695
12696
12697
12698
12699
12700
12701
12702
12703
12704
12705
12706
12707
12708
12709
12710
12711
12712
12713
12714
12715
12716
12717
12718
12719
12720
12721
12722
12723
12724
12725
12726
12727
12728
12729
12730
12731
12732
12733
12734
12735
12736
12737
12738
12739
12740
12741
12742
12743
12744
12745
12746
12747
12748
12749
12750
12751
12752
12753
12754
12755
12756
12757
12758
12759
12760
12761
12762
12763
12764
12765
12766
12767
12768
12769
12770
12771
12772
12773
12774
12775
12776
12777
12778
12779
12780
12781
12782
12783
12784
12785
12786
12787
12788
12789
12790
12791
12792
12793
12794
12795
12796
12797
12798
12799
12800
12801
12802
12803
12804
12805
12806
12807
12808
12809
12810
12811
12812
12813
12814
12815
12816
12817
12818
12819
12820
12821
12822
12823
12824
12825
12826
12827
12828
12829
12830
12831
12832
12833
12834
12835
12836
12837
12838
12839
12840
12841
12842
12843
12844
12845
12846
12847
12848
12849
12850
12851
12852
12853
12854
12855
12856
12857
12858
12859
12860
12861
12862
12863
12864
12865
12866
12867
12868
12869
12870
12871
12872
12873
12874
12875
12876
12877
12878
12879
12880
12881
12882
12883
12884
12885
12886
12887
12888
12889
12890
12891
12892
12893
12894
12895
12896
12897
12898
12899
12900
12901
12902
12903
12904
12905
12906
12907
12908
12909
12910
12911
12912
12913
12914
12915
12916
12917
12918
12919
12920
12921
12922
12923
12924
12925
12926
12927
12928
12929
12930
12931
12932
12933
12934
12935
12936
12937
12938
12939
12940
12941
12942
12943
12944
12945
12946
12947
12948
12949
12950
12951
12952
12953
12954
12955
12956
12957
12958
12959
12960
12961
12962
12963
12964
12965
12966
12967
12968
12969
12970
12971
12972
12973
12974
12975
12976
12977
12978
12979
12980
12981
12982
12983
12984
12985
12986
12987
12988
12989
12990
12991
12992
12993
12994
12995
12996
12997
12998
12999
13000
13001
13002
13003
13004
13005
13006
13007
13008
13009
13010
13011
13012
13013
13014
13015
13016
13017
13018
13019
13020
13021
13022
13023
13024
13025
13026
13027
13028
13029
13030
13031
13032
13033
13034
13035
13036
13037
13038
13039
13040
13041
13042
13043
13044
13045
13046
13047
13048
13049
13050
13051
13052
13053
13054
13055
13056
13057
13058
13059
13060
13061
13062
13063
13064
13065
13066
13067
13068
13069
13070
13071
13072
13073
13074
13075
13076
13077
13078
13079
13080
13081
13082
13083
13084
13085
13086
13087
13088
13089
13090
13091
13092
13093
13094
13095
13096
13097
13098
13099
13100
13101
13102
13103
13104
13105
13106
13107
13108
13109
13110
13111
13112
13113
13114
13115
13116
13117
13118
13119
13120
13121
13122
13123
13124
13125
13126
13127
13128
13129
13130
13131
13132
13133
13134
13135
13136
13137
13138
13139
13140
13141
13142
13143
13144
13145
13146
13147
13148
13149
13150
13151
13152
13153
13154
13155
13156
13157
13158
13159
13160
13161
13162
13163
13164
13165
13166
13167
13168
13169
13170
13171
13172
13173
13174
13175
13176
13177
13178
13179
13180
13181
13182
13183
13184
13185
13186
13187
13188
13189
13190
13191
13192
13193
13194
13195
13196
13197
13198
13199
13200
13201
13202
13203
13204
13205
13206
13207
13208
13209
13210
13211
13212
13213
13214
13215
13216
13217
13218
13219
13220
13221
13222
13223
13224
13225
13226
13227
13228
13229
13230
13231
13232
13233
13234
13235
13236
13237
13238
13239
13240
13241
13242
13243
13244
13245
13246
13247
13248
13249
13250
13251
13252
13253
13254
13255
13256
13257
13258
13259
13260
13261
13262
13263
13264
13265
13266
13267
13268
13269
13270
13271
13272
13273
13274
13275
13276
13277
13278
13279
13280
13281
13282
13283
13284
13285
13286
13287
13288
13289
13290
13291
13292
13293
13294
13295
13296
13297
13298
13299
13300
13301
13302
13303
13304
13305
13306
13307
13308
13309
13310
13311
13312
13313
13314
13315
13316
13317
13318
13319
13320
13321
13322
13323
13324
13325
13326
13327
13328
13329
13330
13331
13332
13333
13334
13335
13336
13337
13338
13339
13340
13341
13342
13343
13344
13345
13346
13347
13348
13349
13350
13351
13352
13353
13354
13355
13356
13357
13358
13359
13360
13361
13362
13363
13364
13365
13366
13367
13368
13369
13370
13371
13372
13373
13374
13375
13376
13377
13378
13379
13380
13381
13382
13383
13384
13385
13386
13387
13388
13389
13390
13391
13392
13393
13394
13395
13396
13397
13398
13399
13400
13401
13402
13403
13404
13405
13406
13407
13408
13409
13410
13411
13412
13413
13414
13415
13416
13417
13418
13419
13420
13421
13422
13423
13424
13425
13426
13427
13428
13429
13430
13431
13432
13433
13434
13435
13436
13437
13438
13439
13440
13441
13442
13443
13444
13445
13446
13447
13448
13449
13450
13451
13452
13453
13454
13455
13456
13457
13458
13459
13460
13461
13462
13463
13464
13465
13466
13467
13468
13469
13470
13471
13472
13473
13474
13475
13476
13477
13478
13479
13480
13481
13482
13483
13484
13485
13486
13487
13488
13489
13490
13491
13492
13493
13494
13495
13496
13497
13498
13499
13500
13501
13502
13503
13504
13505
13506
13507
13508
13509
13510
13511
13512
13513
13514
13515
13516
13517
13518
13519
13520
13521
13522
13523
13524
13525
13526
13527
13528
13529
13530
13531
13532
13533
13534
13535
13536
13537
13538
13539
13540
13541
13542
13543
13544
13545
13546
13547
13548
13549
13550
13551
13552
13553
13554
13555
13556
13557
13558
13559
13560
13561
13562
13563
13564
13565
13566
13567
13568
13569
13570
13571
13572
13573
13574
13575
13576
13577
13578
13579
13580
13581
13582
13583
13584
13585
13586
13587
13588
13589
13590
13591
13592
13593
13594
13595
13596
13597
13598
13599
13600
13601
13602
13603
13604
13605
13606
13607
13608
13609
13610
13611
13612
13613
13614
13615
13616
13617
13618
13619
13620
13621
13622
13623
13624
13625
13626
13627
13628
13629
13630
13631
13632
13633
13634
13635
13636
13637
13638
13639
13640
13641
13642
13643
13644
13645
13646
13647
13648
13649
13650
13651
13652
13653
13654
13655
13656
13657
13658
13659
13660
13661
13662
13663
13664
13665
13666
13667
13668
13669
13670
13671
13672
13673
13674
13675
13676
13677
13678
13679
13680
13681
13682
13683
13684
13685
13686
13687
13688
13689
13690
13691
13692
13693
13694
13695
13696
13697
13698
13699
13700
13701
13702
13703
13704
13705
13706
13707
13708
13709
13710
13711
13712
13713
13714
13715
13716
13717
13718
13719
13720
13721
13722
13723
13724
13725
13726
13727
13728
13729
13730
13731
13732
13733
13734
13735
13736
13737
13738
13739
13740
13741
13742
13743
13744
13745
13746
13747
13748
13749
13750
13751
13752
13753
13754
13755
13756
13757
13758
13759
13760
13761
13762
13763
13764
13765
13766
13767
13768
13769
13770
13771
13772
13773
13774
13775
13776
13777
13778
13779
13780
13781
13782
13783
13784
13785
13786
13787
13788
13789
13790
13791
13792
13793
13794
13795
13796
13797
13798
13799
13800
13801
13802
13803
13804
13805
13806
13807
13808
13809
13810
13811
13812
13813
13814
13815
13816
13817
13818
13819
13820
13821
13822
13823
13824
13825
13826
13827
13828
13829
13830
13831
13832
13833
13834
13835
13836
13837
13838
13839
13840
13841
13842
13843
13844
13845
13846
13847
13848
13849
13850
13851
13852
13853
13854
13855
13856
13857
13858
13859
13860
13861
13862
13863
13864
13865
13866
13867
13868
13869
13870
13871
13872
13873
13874
13875
13876
13877
13878
13879
13880
13881
13882
13883
13884
13885
13886
13887
13888
13889
13890
13891
13892
13893
13894
13895
13896
13897
13898
13899
13900
13901
13902
13903
13904
13905
13906
13907
13908
13909
13910
13911
13912
13913
13914
13915
13916
13917
13918
13919
13920
13921
13922
13923
13924
13925
13926
13927
13928
13929
13930
13931
13932
13933
13934
13935
13936
13937
13938
13939
13940
13941
13942
13943
13944
13945
13946
13947
13948
13949
13950
13951
13952
13953
13954
13955
13956
13957
13958
13959
13960
13961
13962
13963
13964
13965
13966
13967
13968
13969
13970
13971
13972
13973
13974
13975
13976
13977
13978
13979
13980
13981
13982
13983
13984
13985
13986
13987
13988
13989
13990
13991
13992
13993
13994
13995
13996
13997
13998
13999
14000
14001
14002
14003
14004
14005
14006
14007
14008
14009
14010
14011
14012
14013
14014
14015
14016
14017
14018
14019
14020
14021
14022
14023
14024
14025
14026
14027
14028
14029
14030
14031
14032
14033
14034
14035
14036
14037
14038
14039
14040
14041
14042
14043
14044
14045
14046
14047
14048
14049
14050
14051
14052
14053
14054
14055
14056
14057
14058
14059
14060
14061
14062
14063
14064
14065
14066
14067
14068
14069
14070
14071
14072
14073
14074
14075
14076
14077
14078
14079
14080
14081
14082
14083
14084
14085
14086
14087
14088
14089
14090
14091
14092
14093
14094
14095
14096
14097
14098
14099
14100
14101
14102
14103
14104
14105
14106
14107
14108
14109
14110
14111
14112
14113
14114
14115
14116
14117
14118
14119
14120
14121
14122
14123
14124
14125
14126
14127
14128
14129
14130
14131
14132
14133
14134
14135
14136
14137
14138
14139
14140
14141
14142
14143
14144
14145
14146
14147
14148
14149
14150
14151
14152
14153
14154
14155
14156
14157
14158
14159
14160
14161
14162
14163
14164
14165
14166
14167
14168
14169
14170
14171
14172
14173
14174
14175
14176
14177
14178
14179
14180
14181
14182
14183
14184
14185
14186
14187
14188
14189
14190
14191
14192
14193
14194
14195
14196
14197
14198
14199
14200
14201
14202
14203
14204
14205
14206
14207
14208
14209
14210
14211
14212
14213
14214
14215
14216
14217
14218
14219
14220
14221
14222
14223
14224
14225
14226
14227
14228
14229
14230
14231
14232
14233
14234
14235
14236
14237
14238
14239
14240
14241
14242
14243
14244
14245
14246
14247
14248
14249
14250
14251
14252
14253
14254
14255
14256
14257
14258
14259
14260
14261
14262
14263
14264
14265
14266
14267
14268
14269
14270
14271
14272
14273
14274
14275
14276
14277
14278
14279
14280
14281
14282
14283
14284
14285
14286
14287
14288
14289
14290
14291
14292
14293
14294
14295
14296
14297
14298
14299
14300
14301
14302
14303
14304
14305
14306
14307
14308
14309
14310
14311
14312
14313
14314
14315
14316
14317
14318
14319
14320
14321
14322
14323
14324
14325
14326
14327
14328
14329
14330
14331
14332
14333
14334
14335
14336
14337
14338
14339
14340
14341
14342
14343
14344
14345
14346
14347
14348
14349
14350
14351
14352
14353
14354
14355
14356
14357
14358
14359
14360
14361
14362
14363
14364
14365
14366
14367
14368
14369
14370
14371
14372
14373
14374
14375
14376
14377
14378
14379
14380
14381
14382
14383
14384
14385
14386
14387
14388
14389
14390
14391
14392
14393
14394
14395
14396
14397
14398
14399
14400
14401
14402
14403
14404
14405
14406
14407
14408
14409
14410
14411
14412
14413
14414
14415
14416
14417
14418
14419
14420
14421
14422
14423
14424
14425
14426
14427
14428
14429
14430
14431
14432
14433
14434
14435
14436
14437
14438
14439
14440
14441
14442
14443
14444
14445
14446
14447
14448
14449
14450
14451
14452
14453
14454
14455
14456
14457
14458
14459
14460
14461
14462
14463
14464
14465
14466
14467
14468
14469
14470
14471
14472
14473
14474
14475
14476
14477
14478
14479
14480
14481
14482
14483
14484
14485
14486
14487
14488
14489
14490
14491
14492
14493
14494
14495
14496
14497
14498
14499
14500
14501
14502
14503
14504
14505
14506
14507
14508
14509
14510
14511
14512
14513
14514
14515
14516
14517
14518
14519
14520
14521
14522
14523
14524
14525
14526
14527
14528
14529
14530
14531
14532
14533
14534
14535
14536
14537
14538
14539
14540
14541
14542
14543
14544
14545
14546
14547
14548
14549
14550
14551
14552
14553
14554
14555
14556
14557
14558
14559
14560
14561
14562
14563
14564
14565
14566
14567
14568
14569
14570
14571
14572
14573
14574
14575
14576
14577
14578
14579
14580
14581
14582
14583
14584
14585
14586
14587
14588
14589
14590
14591
14592
14593
14594
14595
14596
14597
14598
14599
14600
14601
14602
14603
14604
14605
14606
14607
14608
14609
14610
14611
14612
14613
14614
14615
14616
14617
14618
14619
14620
14621
14622
14623
14624
14625
14626
14627
14628
14629
14630
14631
14632
14633
14634
14635
14636
14637
14638
14639
14640
14641
14642
14643
14644
14645
14646
14647
14648
14649
14650
14651
14652
14653
14654
14655
14656
14657
14658
14659
14660
14661
14662
14663
14664
14665
14666
14667
14668
14669
14670
14671
14672
14673
14674
14675
14676
14677
14678
14679
14680
14681
14682
14683
14684
14685
14686
14687
14688
14689
14690
14691
14692
14693
14694
14695
14696
14697
14698
14699
14700
14701
14702
14703
14704
14705
14706
14707
14708
14709
14710
14711
14712
14713
14714
14715
14716
14717
14718
14719
14720
14721
14722
14723
14724
14725
14726
14727
14728
14729
14730
14731
14732
14733
14734
14735
14736
14737
14738
14739
14740
14741
14742
14743
14744
14745
14746
14747
14748
14749
14750
14751
14752
14753
14754
14755
14756
14757
14758
14759
14760
14761
14762
14763
14764
14765
14766
14767
14768
14769
14770
14771
14772
14773
14774
14775
14776
14777
14778
14779
14780
14781
14782
14783
14784
14785
14786
14787
14788
14789
14790
14791
14792
14793
14794
14795
14796
14797
14798
14799
14800
14801
14802
14803
14804
14805
14806
14807
14808
14809
14810
14811
14812
14813
14814
14815
14816
14817
14818
14819
14820
14821
14822
14823
14824
14825
14826
14827
14828
14829
14830
14831
14832
14833
14834
14835
14836
14837
14838
14839
14840
14841
14842
14843
14844
14845
14846
14847
14848
14849
14850
14851
14852
14853
14854
14855
14856
14857
14858
14859
14860
14861
14862
14863
14864
14865
14866
14867
14868
14869
14870
14871
14872
14873
14874
14875
14876
14877
14878
14879
14880
14881
14882
14883
14884
14885
14886
14887
14888
14889
14890
14891
14892
14893
14894
14895
14896
14897
14898
14899
14900
14901
14902
14903
14904
14905
14906
14907
14908
14909
14910
14911
14912
14913
14914
14915
14916
14917
14918
14919
14920
14921
14922
14923
14924
14925
14926
14927
14928
14929
14930
14931
14932
14933
14934
14935
14936
14937
14938
14939
14940
14941
14942
14943
14944
14945
14946
14947
14948
14949
14950
14951
14952
14953
14954
14955
14956
14957
14958
14959
14960
14961
14962
14963
14964
14965
14966
14967
14968
14969
14970
14971
14972
14973
14974
14975
14976
14977
14978
14979
14980
14981
14982
14983
14984
14985
14986
14987
14988
14989
14990
14991
14992
14993
14994
14995
14996
14997
14998
14999
15000
15001
15002
15003
15004
15005
15006
15007
15008
15009
15010
15011
15012
15013
15014
15015
15016
15017
15018
15019
15020
15021
15022
15023
15024
15025
15026
15027
15028
15029
15030
15031
15032
15033
15034
15035
15036
15037
15038
15039
15040
15041
15042
15043
15044
15045
15046
15047
15048
15049
15050
15051
15052
15053
15054
15055
15056
15057
15058
15059
15060
15061
15062
15063
15064
15065
15066
15067
15068
15069
15070
15071
15072
15073
15074
15075
15076
15077
15078
15079
15080
15081
15082
15083
15084
15085
15086
15087
15088
15089
15090
15091
15092
15093
15094
15095
15096
15097
15098
15099
15100
15101
15102
15103
15104
15105
15106
15107
15108
15109
15110
15111
15112
15113
15114
15115
15116
15117
15118
15119
15120
15121
15122
15123
15124
15125
15126
15127
15128
15129
15130
15131
15132
15133
15134
15135
15136
15137
15138
15139
15140
15141
15142
15143
15144
15145
15146
15147
15148
15149
15150
15151
15152
15153
15154
15155
15156
15157
15158
15159
15160
15161
15162
15163
15164
15165
15166
15167
15168
15169
15170
15171
15172
15173
15174
15175
15176
15177
15178
15179
15180
15181
15182
15183
15184
15185
15186
15187
15188
15189
15190
15191
15192
15193
15194
15195
15196
15197
15198
15199
15200
15201
15202
15203
15204
15205
15206
15207
15208
15209
15210
15211
15212
15213
15214
15215
15216
15217
15218
15219
15220
15221
15222
15223
15224
15225
15226
15227
15228
15229
15230
15231
15232
15233
15234
15235
15236
15237
15238
15239
15240
15241
15242
15243
15244
15245
15246
15247
15248
15249
15250
15251
15252
15253
15254
15255
15256
15257
15258
15259
15260
15261
15262
15263
15264
15265
15266
15267
15268
15269
15270
15271
15272
15273
15274
15275
15276
15277
15278
15279
15280
15281
15282
15283
15284
15285
15286
15287
15288
15289
15290
15291
15292
15293
15294
15295
15296
15297
15298
15299
15300
15301
15302
15303
15304
15305
15306
15307
15308
15309
15310
15311
15312
15313
15314
15315
15316
15317
15318
15319
15320
15321
15322
15323
15324
15325
15326
15327
15328
15329
15330
15331
15332
15333
15334
15335
15336
15337
15338
15339
15340
15341
15342
15343
15344
15345
15346
15347
15348
15349
15350
15351
15352
15353
15354
15355
15356
15357
15358
15359
15360
15361
15362
15363
15364
15365
15366
15367
15368
15369
15370
15371
15372
15373
15374
15375
15376
15377
15378
15379
15380
15381
15382
15383
15384
15385
15386
15387
15388
15389
15390
15391
15392
15393
15394
15395
15396
15397
15398
15399
15400
15401
15402
15403
15404
15405
15406
15407
15408
15409
15410
15411
15412
15413
15414
15415
15416
15417
15418
15419
15420
15421
15422
15423
15424
15425
15426
15427
15428
15429
15430
15431
15432
15433
15434
15435
15436
15437
15438
15439
15440
15441
15442
15443
15444
15445
15446
15447
15448
15449
15450
15451
15452
15453
15454
15455
15456
15457
15458
15459
15460
15461
15462
15463
15464
15465
15466
15467
15468
15469
15470
15471
15472
15473
15474
15475
15476
15477
15478
15479
15480
15481
15482
15483
15484
15485
15486
15487
15488
15489
15490
15491
15492
15493
15494
15495
15496
15497
15498
15499
15500
15501
15502
15503
15504
15505
15506
15507
15508
15509
15510
15511
15512
15513
15514
15515
15516
15517
15518
15519
15520
15521
15522
15523
15524
15525
15526
15527
15528
15529
15530
15531
15532
15533
15534
15535
15536
15537
15538
15539
15540
15541
15542
15543
15544
15545
15546
15547
15548
15549
15550
15551
15552
15553
15554
15555
15556
15557
15558
15559
15560
15561
15562
15563
15564
15565
15566
15567
15568
15569
15570
15571
15572
15573
15574
15575
15576
15577
15578
15579
15580
15581
15582
15583
15584
15585
15586
15587
15588
15589
15590
15591
15592
15593
15594
15595
15596
15597
15598
15599
15600
15601
15602
15603
15604
15605
15606
15607
15608
15609
15610
15611
15612
15613
15614
15615
15616
15617
15618
15619
15620
15621
15622
15623
15624
15625
15626
15627
15628
15629
15630
15631
15632
15633
15634
15635
15636
15637
15638
15639
15640
15641
15642
15643
15644
15645
15646
15647
15648
15649
15650
15651
15652
15653
15654
15655
15656
15657
15658
15659
15660
15661
15662
15663
15664
15665
15666
15667
15668
15669
15670
15671
15672
15673
15674
15675
15676
15677
15678
15679
15680
15681
15682
15683
15684
15685
15686
15687
15688
15689
15690
15691
15692
15693
15694
15695
15696
15697
15698
15699
15700
15701
15702
15703
15704
15705
15706
15707
15708
15709
15710
15711
15712
15713
15714
15715
15716
15717
15718
15719
15720
15721
15722
15723
15724
15725
15726
15727
15728
15729
15730
15731
15732
15733
15734
15735
15736
15737
15738
15739
15740
15741
15742
15743
15744
15745
15746
15747
15748
15749
15750
15751
15752
15753
15754
15755
15756
15757
15758
15759
15760
15761
15762
15763
15764
15765
15766
15767
15768
15769
15770
15771
15772
15773
15774
15775
15776
15777
15778
15779
15780
15781
15782
15783
15784
15785
15786
15787
15788
15789
15790
15791
15792
15793
15794
15795
15796
15797
15798
15799
15800
15801
15802
15803
15804
15805
15806
15807
15808
15809
15810
15811
15812
15813
15814
15815
15816
15817
15818
15819
15820
15821
15822
15823
15824
15825
15826
15827
15828
15829
15830
15831
15832
15833
15834
15835
15836
15837
15838
15839
;;; GNU Guix --- Functional package management for GNU
;;; Copyright © 2014, 2015, 2016, 2017, 2018, 2019, 2020 Ricardo Wurmus <rekado@elephly.net>
;;; Copyright © 2015, 2016, 2017, 2018 Ben Woodcroft <donttrustben@gmail.com>
;;; Copyright © 2015, 2016, 2018, 2019, 2020 Pjotr Prins <pjotr.guix@thebird.nl>
;;; Copyright © 2015 Andreas Enge <andreas@enge.fr>
;;; Copyright © 2016, 2020 Roel Janssen <roel@gnu.org>
;;; Copyright © 2016, 2017, 2018, 2019, 2020 Efraim Flashner <efraim@flashner.co.il>
;;; Copyright © 2016, 2020 Marius Bakke <mbakke@fastmail.com>
;;; Copyright © 2016, 2018 Raoul Bonnal <ilpuccio.febo@gmail.com>
;;; Copyright © 2017, 2018 Tobias Geerinckx-Rice <me@tobias.gr>
;;; Copyright © 2017 Arun Isaac <arunisaac@systemreboot.net>
;;; Copyright © 2018 Joshua Sierles, Nextjournal <joshua@nextjournal.com>
;;; Copyright © 2018 Gábor Boskovits <boskovits@gmail.com>
;;; Copyright © 2018, 2019, 2020 Mădălin Ionel Patrașcu <madalinionel.patrascu@mdc-berlin.de>
;;; Copyright © 2019, 2020 Maxim Cournoyer <maxim.cournoyer@gmail.com>
;;; Copyright © 2019 Brian Leung <bkleung89@gmail.com>
;;; Copyright © 2019 Brett Gilio <brettg@gnu.org>
;;; Copyright © 2020 Björn Höfling <bjoern.hoefling@bjoernhoefling.de>
;;; Copyright © 2020 Jakub Kądziołka <kuba@kadziolka.net>
;;; Copyright © 2020 Pierre Langlois <pierre.langlois@gmx.com>
;;; Copyright © 2020 Bonface Munyoki Kilyungi <bonfacemunyoki@gmail.com>
;;;
;;; This file is part of GNU Guix.
;;;
;;; GNU Guix is free software; you can redistribute it and/or modify it
;;; under the terms of the GNU General Public License as published by
;;; the Free Software Foundation; either version 3 of the License, or (at
;;; your option) any later version.
;;;
;;; GNU Guix is distributed in the hope that it will be useful, but
;;; WITHOUT ANY WARRANTY; without even the implied warranty of
;;; MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
;;; GNU General Public License for more details.
;;;
;;; You should have received a copy of the GNU General Public License
;;; along with GNU Guix.  If not, see <http://www.gnu.org/licenses/>.

(define-module (gnu packages bioinformatics)
  #:use-module ((guix licenses) #:prefix license:)
  #:use-module (guix packages)
  #:use-module (guix utils)
  #:use-module (guix download)
  #:use-module (guix git-download)
  #:use-module (guix hg-download)
  #:use-module (guix build-system ant)
  #:use-module (guix build-system gnu)
  #:use-module (guix build-system cmake)
  #:use-module (guix build-system go)
  #:use-module (guix build-system haskell)
  #:use-module (guix build-system meson)
  #:use-module (guix build-system ocaml)
  #:use-module (guix build-system perl)
  #:use-module (guix build-system python)
  #:use-module (guix build-system r)
  #:use-module (guix build-system ruby)
  #:use-module (guix build-system scons)
  #:use-module (guix build-system trivial)
  #:use-module (guix deprecation)
  #:use-module (gnu packages)
  #:use-module (gnu packages autotools)
  #:use-module (gnu packages algebra)
  #:use-module (gnu packages base)
  #:use-module (gnu packages bash)
  #:use-module (gnu packages bison)
  #:use-module (gnu packages bioconductor)
  #:use-module (gnu packages boost)
  #:use-module (gnu packages check)
  #:use-module (gnu packages code)
  #:use-module (gnu packages commencement)
  #:use-module (gnu packages cmake)
  #:use-module (gnu packages compression)
  #:use-module (gnu packages cpio)
  #:use-module (gnu packages cran)
  #:use-module (gnu packages curl)
  #:use-module (gnu packages documentation)
  #:use-module (gnu packages databases)
  #:use-module (gnu packages datastructures)
  #:use-module (gnu packages dlang)
  #:use-module (gnu packages file)
  #:use-module (gnu packages flex)
  #:use-module (gnu packages gawk)
  #:use-module (gnu packages gcc)
  #:use-module (gnu packages gd)
  #:use-module (gnu packages golang)
  #:use-module (gnu packages glib)
  #:use-module (gnu packages graph)
  #:use-module (gnu packages graphics)
  #:use-module (gnu packages graphviz)
  #:use-module (gnu packages groff)
  #:use-module (gnu packages gtk)
  #:use-module (gnu packages guile)
  #:use-module (gnu packages guile-xyz)
  #:use-module (gnu packages haskell-check)
  #:use-module (gnu packages haskell-web)
  #:use-module (gnu packages haskell-xyz)
  #:use-module (gnu packages image)
  #:use-module (gnu packages image-processing)
  #:use-module (gnu packages imagemagick)
  #:use-module (gnu packages java)
  #:use-module (gnu packages java-compression)
  #:use-module (gnu packages jemalloc)
  #:use-module (gnu packages linux)
  #:use-module (gnu packages lisp-xyz)
  #:use-module (gnu packages logging)
  #:use-module (gnu packages machine-learning)
  #:use-module (gnu packages man)
  #:use-module (gnu packages maths)
  #:use-module (gnu packages mpi)
  #:use-module (gnu packages ncurses)
  #:use-module (gnu packages node)
  #:use-module (gnu packages ocaml)
  #:use-module (gnu packages pcre)
  #:use-module (gnu packages parallel)
  #:use-module (gnu packages pdf)
  #:use-module (gnu packages perl)
  #:use-module (gnu packages perl-check)
  #:use-module (gnu packages pkg-config)
  #:use-module (gnu packages popt)
  #:use-module (gnu packages protobuf)
  #:use-module (gnu packages python)
  #:use-module (gnu packages python-check)
  #:use-module (gnu packages python-compression)
  #:use-module (gnu packages python-science)
  #:use-module (gnu packages python-web)
  #:use-module (gnu packages python-xyz)
  #:use-module (gnu packages rdf)
  #:use-module (gnu packages readline)
  #:use-module (gnu packages ruby)
  #:use-module (gnu packages serialization)
  #:use-module (gnu packages shells)
  #:use-module (gnu packages sphinx)
  #:use-module (gnu packages statistics)
  #:use-module (gnu packages swig)
  #:use-module (gnu packages tbb)
  #:use-module (gnu packages tex)
  #:use-module (gnu packages texinfo)
  #:use-module (gnu packages textutils)
  #:use-module (gnu packages time)
  #:use-module (gnu packages tls)
  #:use-module (gnu packages vim)
  #:use-module (gnu packages web)
  #:use-module (gnu packages xml)
  #:use-module (gnu packages xorg)
  #:use-module (srfi srfi-1)
  #:use-module (ice-9 match))

(define-public aragorn
  (package
    (name "aragorn")
    (version "1.2.38")
    (source (origin
              (method url-fetch)
              (uri (string-append
                    "http://mbio-serv2.mbioekol.lu.se/ARAGORN/Downloads/aragorn"
                    version ".tgz"))
              (sha256
               (base32
                "09i1rg716smlbnixfm7q1ml2mfpaa2fpn3hwjg625ysmfwwy712b"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; there are no tests
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (replace 'build
           (lambda _
             (invoke "gcc"
                     "-O3"
                     "-ffast-math"
                     "-finline-functions"
                     "-o"
                     "aragorn"
                     (string-append "aragorn" ,version ".c"))
             #t))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin"))
                    (man (string-append out "/share/man/man1")))
               (install-file "aragorn" bin)
               (install-file "aragorn.1" man))
             #t)))))
    (home-page "http://mbio-serv2.mbioekol.lu.se/ARAGORN")
    (synopsis "Detect tRNA, mtRNA and tmRNA genes in nucleotide sequences")
    (description
     "Aragorn identifies transfer RNA, mitochondrial RNA and
transfer-messenger RNA from nucleotide sequences, based on homology to known
tRNA consensus sequences and RNA structure.  It also outputs the secondary
structure of the predicted RNA.")
    (license license:gpl2)))

(define-public bamm
  (package
    (name "bamm")
    (version "1.7.3")
    (source (origin
              (method git-fetch)
              ;; BamM is not available on pypi.
              (uri (git-reference
                    (url "https://github.com/Ecogenomics/BamM")
                    (commit version)
                    (recursive? #t)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1p83ahi984ipslxlg4yqy1gdnya9rkn1v71z8djgxkm9d2chw4c5"))
              (modules '((guix build utils)))
              (snippet
               `(begin
                  ;; Delete bundled htslib.
                  (delete-file-recursively "c/htslib-1.3.1")
                  #t))))
    (build-system python-build-system)
    (arguments
     `(#:python ,python-2 ; BamM is Python 2 only.
       ;; Do not use bundled libhts.  Do use the bundled libcfu because it has
       ;; been modified from its original form.
       #:configure-flags
       (let ((htslib (assoc-ref %build-inputs "htslib")))
         (list "--with-libhts-lib" (string-append htslib "/lib")
               "--with-libhts-inc" (string-append htslib "/include/htslib")))
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'autogen
           (lambda _
             (with-directory-excursion "c"
               (let ((sh (which "sh")))
                 (for-each make-file-writable (find-files "." ".*"))
                 ;; Use autogen so that 'configure' works.
                 (substitute* "autogen.sh" (("/bin/sh") sh))
                 (setenv "CONFIG_SHELL" sh)
                 (invoke "./autogen.sh")))
             #t))
         (delete 'build)
         ;; Run tests after installation so compilation only happens once.
         (delete 'check)
         (add-after 'install 'wrap-executable
           (lambda* (#:key outputs #:allow-other-keys)
            (let* ((out  (assoc-ref outputs "out"))
                   (path (getenv "PATH")))
              (wrap-program (string-append out "/bin/bamm")
                `("PATH" ":" prefix (,path))))
            #t))
         (add-after 'wrap-executable 'post-install-check
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (setenv "PATH"
                     (string-append (assoc-ref outputs "out")
                                    "/bin:"
                                    (getenv "PATH")))
             (setenv "PYTHONPATH"
                     (string-append
                      (assoc-ref outputs "out")
                      "/lib/python"
                      (string-take (string-take-right
                                    (assoc-ref inputs "python") 5) 3)
                      "/site-packages:"
                      (getenv "PYTHONPATH")))
             ;; There are 2 errors printed, but they are safe to ignore:
             ;; 1) [E::hts_open_format] fail to open file ...
             ;; 2) samtools view: failed to open ...
             (invoke "nosetests")
             #t)))))
    (native-inputs
     `(("autoconf" ,autoconf)
       ("automake" ,automake)
       ("libtool" ,libtool)
       ("zlib" ,zlib)
       ("python-nose" ,python2-nose)
       ("python-pysam" ,python2-pysam)))
    (inputs
     `(("htslib" ,htslib-1.3) ; At least one test fails on htslib-1.4+.
       ("samtools" ,samtools)
       ("bwa" ,bwa)
       ("grep" ,grep)
       ("sed" ,sed)
       ("coreutils" ,coreutils)))
    (propagated-inputs
     `(("python-numpy" ,python2-numpy)))
    (home-page "https://ecogenomics.github.io/BamM/")
    (synopsis "Metagenomics-focused BAM file manipulator")
    (description
     "BamM is a C library, wrapped in python, to efficiently generate and
parse BAM files, specifically for the analysis of metagenomic data.  For
instance, it implements several methods to assess contig-wise read coverage.")
    (license license:lgpl3+)))

(define-public bamtools
  (package
    (name "bamtools")
    (version "2.5.1")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/pezmaster31/bamtools")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "0nfb2ypcx9959xnbz6wxh6py3xfizgmg8nrknxl95c507m9hmq8b"))))
    (build-system cmake-build-system)
    (arguments
     `(#:tests? #f ;no "check" target
       #:phases
       (modify-phases %standard-phases
         (add-before
          'configure 'set-ldflags
          (lambda* (#:key outputs #:allow-other-keys)
            (setenv "LDFLAGS"
                    (string-append
                     "-Wl,-rpath="
                     (assoc-ref outputs "out") "/lib/bamtools"))
            #t)))))
    (inputs `(("zlib" ,zlib)))
    (home-page "https://github.com/pezmaster31/bamtools")
    (synopsis "C++ API and command-line toolkit for working with BAM data")
    (description
     "BamTools provides both a C++ API and a command-line toolkit for handling
BAM files.")
    (license license:expat)))

(define-public bcftools
  (package
    (name "bcftools")
    (version "1.11")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://github.com/samtools/bcftools/"
                                  "releases/download/"
                                  version "/bcftools-" version ".tar.bz2"))
              (sha256
               (base32
                "0r508mp15pqzf8r1269kb4v5naw9zsvbwd3cz8s1yj7carsf9viw"))
              (modules '((guix build utils)))
              (snippet '(begin
                          ;; Delete bundled htslib.
                          (delete-file-recursively "htslib-1.11")
                          #t))))
    (build-system gnu-build-system)
    (arguments
     `(#:configure-flags
       (list "--enable-libgsl")
       #:test-target "test"
       #:phases
       (modify-phases %standard-phases
         (add-before 'check 'patch-tests
           (lambda _
             (substitute* "test/test.pl"
               (("/bin/bash") (which "bash")))
             #t)))))
    (native-inputs
     `(("htslib" ,htslib)
       ("perl" ,perl)))
    (inputs
     `(("gsl" ,gsl)
       ("zlib" ,zlib)))
    (home-page "https://samtools.github.io/bcftools/")
    (synopsis "Utilities for variant calling and manipulating VCFs and BCFs")
    (description
     "BCFtools is a set of utilities that manipulate variant calls in the
Variant Call Format (VCF) and its binary counterpart BCF.  All commands work
transparently with both VCFs and BCFs, both uncompressed and BGZF-compressed.")
    ;; The sources are dual MIT/GPL, but becomes GPL-only when USE_GPL=1.
    (license (list license:gpl3+ license:expat))))

(define-public bcftools-1.9
  (package (inherit bcftools)
    (name "bcftools")
    (version "1.9")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://github.com/samtools/bcftools/"
                                  "releases/download/"
                                  version "/bcftools-" version ".tar.bz2"))
              (sha256
               (base32
                "1j3h638i8kgihzyrlnpj82xg1b23sijibys9hvwari3fy7kd0dkg"))
              (modules '((guix build utils)))
              (snippet '(begin
                          ;; Delete bundled htslib.
                          (delete-file-recursively "htslib-1.9")
                          #t))))
    (build-system gnu-build-system)
    (native-inputs
     `(("htslib" ,htslib-1.9)
       ("perl" ,perl)))))

(define-public bedops
  (package
    (name "bedops")
    (version "2.4.35")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/bedops/bedops")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "0mmgsgwz5r9w76hzgxkxc9s9lkdhhaf7vr6i02b09vbswvs1fyqx"))))
    (build-system gnu-build-system)
    (arguments
     '(#:tests? #f
       #:make-flags (list (string-append "BINDIR=" %output "/bin"))
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'unpack-tarballs
           (lambda _
             ;; FIXME: Bedops includes tarballs of minimally patched upstream
             ;; libraries jansson, zlib, and bzip2.  We cannot just use stock
             ;; libraries because at least one of the libraries (zlib) is
             ;; patched to add a C++ function definition (deflateInit2cpp).
             ;; Until the Bedops developers offer a way to link against system
             ;; libraries we have to build the in-tree copies of these three
             ;; libraries.

             ;; See upstream discussion:
             ;; https://github.com/bedops/bedops/issues/124

             ;; Unpack the tarballs to benefit from shebang patching.
             (with-directory-excursion "third-party"
               (invoke "tar" "xvf" "jansson-2.6.tar.bz2")
               (invoke "tar" "xvf" "zlib-1.2.7.tar.bz2")
               (invoke "tar" "xvf" "bzip2-1.0.6.tar.bz2"))
             ;; Disable unpacking of tarballs in Makefile.
             (substitute* "system.mk/Makefile.linux"
               (("^\tbzcat .*") "\t@echo \"not unpacking\"\n")
               (("\\./configure") "CONFIG_SHELL=bash ./configure"))
             (substitute* "third-party/zlib-1.2.7/Makefile.in"
               (("^SHELL=.*$") "SHELL=bash\n"))
             #t))
         (delete 'configure))))
    (home-page "https://github.com/bedops/bedops")
    (synopsis "Tools for high-performance genomic feature operations")
    (description
     "BEDOPS is a suite of tools to address common questions raised in genomic
studies---mostly with regard to overlap and proximity relationships between
data sets.  It aims to be scalable and flexible, facilitating the efficient
and accurate analysis and management of large-scale genomic data.

BEDOPS provides tools that perform highly efficient and scalable Boolean and
other set operations, statistical calculations, archiving, conversion and
other management of genomic data of arbitrary scale.  Tasks can be easily
split by chromosome for distributing whole-genome analyses across a
computational cluster.")
    (license license:gpl2+)))

(define-public bedtools
  (package
    (name "bedtools")
    (version "2.29.2")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://github.com/arq5x/bedtools2/releases/"
                                  "download/v" version "/"
                                  "bedtools-" version ".tar.gz"))
              (sha256
               (base32
                "0m3hk6548846w83a9s5drsczvy67n2azx41kj71n03klb2gbzwg3"))))
    (build-system gnu-build-system)
    (arguments
     '(#:test-target "test"
       #:make-flags
       (list (string-append "prefix=" (assoc-ref %outputs "out")))
       #:phases
       (modify-phases %standard-phases
         (delete 'configure))))
    (native-inputs
     `(("python" ,python-wrapper)))
    (inputs
     `(("samtools" ,samtools-1.9)
       ("zlib" ,zlib)))
    (home-page "https://github.com/arq5x/bedtools2")
    (synopsis "Tools for genome analysis and arithmetic")
    (description
     "Collectively, the bedtools utilities are a swiss-army knife of tools for
a wide-range of genomics analysis tasks.  The most widely-used tools enable
genome arithmetic: that is, set theory on the genome.  For example, bedtools
allows one to intersect, merge, count, complement, and shuffle genomic
intervals from multiple files in widely-used genomic file formats such as BAM,
BED, GFF/GTF, VCF.")
    (license license:expat)))

;; Later releases of bedtools produce files with more columns than
;; what Ribotaper expects.
(define-public bedtools-2.18
  (package (inherit bedtools)
    (name "bedtools")
    (version "2.18.0")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://github.com/arq5x/bedtools2/"
                                  "releases/download/v" version
                                  "/bedtools-" version ".tar.gz"))
              (sha256
               (base32
                "11rvca19ncg03kxd0wzlfx5ws7r3nisd0z8s9j9n182d8ksp2pxz"))))
    (arguments
     '(#:test-target "test"
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((bin (string-append (assoc-ref outputs "out") "/bin/")))
               (for-each (lambda (file)
                           (install-file file bin))
                         (find-files "bin" ".*")))
             #t)))))))

(define-public pbbam
  (package
    (name "pbbam")
    (version "0.23.0")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/PacificBiosciences/pbbam")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "0h9gkrpf2lrxklxp72xfl5bi3h5zcm5hprrya9gf0hr3xwlbpp0x"))))
    (build-system meson-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'find-googletest
           (lambda* (#:key inputs #:allow-other-keys)
             ;; It doesn't find gtest_main because there's no pkg-config file
             ;; for it.  Find it another way.
             (substitute* "tests/meson.build"
               (("pbbam_gtest_dep = dependency\\('gtest_main'.*")
                (format #f "cpp = meson.get_compiler('cpp')
pbbam_gtest_dep = cpp.find_library('gtest_main', dirs : '~a')\n"
                        (assoc-ref inputs "googletest"))))
             #t)))
       ;; TODO: tests/pbbam_test cannot be linked
       ;; ld: tests/59830eb@@pbbam_test@exe/src_test_Accuracy.cpp.o:
       ;;   undefined reference to symbol '_ZTIN7testing4TestE'
       ;; ld: /gnu/store/...-googletest-1.8.0/lib/libgtest.so:
       ;;   error adding symbols: DSO missing from command line
       #:tests? #f
       #:configure-flags '("-Dtests=false")))
    ;; These libraries are listed as "Required" in the pkg-config file.
    (propagated-inputs
     `(("htslib" ,htslib)
       ("zlib" ,zlib)))
    (inputs
     `(("boost" ,boost)
       ("samtools" ,samtools)))
    (native-inputs
     `(("googletest" ,googletest)
       ("pkg-config" ,pkg-config)
       ("python" ,python-wrapper))) ; for tests
    (home-page "https://github.com/PacificBiosciences/pbbam")
    (synopsis "Work with PacBio BAM files")
    (description
     "The pbbam software package provides components to create, query, and
edit PacBio BAM files and associated indices.  These components include a core
C++ library, bindings for additional languages, and command-line utilities.
This library is not intended to be used as a general-purpose BAM utility - all
input and output BAMs must adhere to the PacBio BAM format specification.
Non-PacBio BAMs will cause exceptions to be thrown.")
    (license license:bsd-3)))

(define-public blasr-libcpp
  (package
    (name "blasr-libcpp")
    (version "5.3.3")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/PacificBiosciences/blasr_libcpp")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "0cn5l42zyq67sj0g2imqkhayz2iqvv0a1pgpbmlq0qynjmsrbfd2"))))
    (build-system meson-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'link-with-hdf5
           (lambda* (#:key inputs #:allow-other-keys)
             (let ((hdf5 (assoc-ref inputs "hdf5")))
               (substitute* "meson.build"
                 (("libblasr_deps = \\[" m)
                  (string-append
                   m
                   (format #f "cpp.find_library('hdf5', dirs : '~a'), \
cpp.find_library('hdf5_cpp', dirs : '~a'), "
                           hdf5 hdf5)))))
             #t))
         (add-after 'unpack 'find-googletest
           (lambda* (#:key inputs #:allow-other-keys)
             ;; It doesn't find gtest_main because there's no pkg-config file
             ;; for it.  Find it another way.
             (substitute* "unittest/meson.build"
               (("libblasr_gtest_dep = dependency\\('gtest_main'.*")
                (format #f "cpp = meson.get_compiler('cpp')
libblasr_gtest_dep = cpp.find_library('gtest_main', dirs : '~a')\n"
                        (assoc-ref inputs "googletest"))))
             #t)))
       ;; TODO: unittest/libblasr_unittest cannot be linked
       ;; ld: ;; unittest/df08227@@libblasr_unittest@exe/alignment_utils_FileUtils_gtest.cpp.o:
       ;; undefined reference to symbol
       ;; '_ZN7testing8internal9DeathTest6CreateEPKcPKNS0_2REES3_iPPS1_'
       ;; ld: /gnu/store/...-googletest-1.8.0/lib/libgtest.so:
       ;;   error adding symbols: DSO missing from command line
       #:tests? #f
       #:configure-flags '("-Dtests=false")))
    (inputs
     `(("boost" ,boost)
       ("hdf5" ,hdf5)
       ("pbbam" ,pbbam)
       ("zlib" ,zlib)))
    (native-inputs
     `(("googletest" ,googletest)
       ("pkg-config" ,pkg-config)))
    (home-page "https://github.com/PacificBiosciences/blasr_libcpp")
    (synopsis "Library for analyzing PacBio genomic sequences")
    (description
     "This package provides three libraries used by applications for analyzing
PacBio genomic sequences.  This library contains three sub-libraries: pbdata,
hdf and alignment.")
    (license license:bsd-3)))

(define-public blasr
  (package
    (name "blasr")
    (version "5.3.3")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/PacificBiosciences/blasr")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1skgy2mvz8gsgfh1gc2nfgwvpyzb1hpmp2cf2773h5wsj8nw22kl"))))
    (build-system meson-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'link-with-hdf5
           (lambda* (#:key inputs #:allow-other-keys)
             (let ((hdf5 (assoc-ref inputs "hdf5")))
               (substitute* "meson.build"
                 (("blasr_deps = \\[" m)
                  (string-append
                   m
                   (format #f "cpp.find_library('hdf5', dirs : '~a'), \
cpp.find_library('hdf5_cpp', dirs : '~a'), "
                           hdf5 hdf5)))))
             #t)))
       ;; Tests require "cram" executable, which is not packaged.
       #:tests? #f
       #:configure-flags '("-Dtests=false")))
    (inputs
     `(("boost" ,boost)
       ("blasr-libcpp" ,blasr-libcpp)
       ("hdf5" ,hdf5)
       ("pbbam" ,pbbam)
       ("zlib" ,zlib)))
    (native-inputs
     `(("pkg-config" ,pkg-config)))
    (home-page "https://github.com/PacificBiosciences/blasr")
    (synopsis "PacBio long read aligner")
    (description
     "Blasr is a genomic sequence aligner for processing PacBio long reads.")
    (license license:bsd-3)))

(define-public ribotaper
  (package
    (name "ribotaper")
    (version "1.3.1")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://ohlerlab.mdc-berlin.de/"
                                  "files/RiboTaper/RiboTaper_Version_"
                                  version ".tar.gz"))
              (sha256
               (base32
                "0ykjbps1y3z3085q94npw8i9x5gldc6shy8vlc08v76zljsm07hv"))))
    (build-system gnu-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'install 'wrap-executables
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out")))
               (for-each
                (lambda (script)
                  (wrap-program (string-append out "/bin/" script)
                    `("R_LIBS_SITE" ":" = (,(getenv "R_LIBS_SITE")))))
                '("create_annotations_files.bash"
                  "create_metaplots.bash"
                  "Ribotaper_ORF_find.sh"
                  "Ribotaper.sh")))
             #t)))))
    (inputs
     `(("bedtools" ,bedtools-2.18)
       ("samtools" ,samtools-0.1)
       ("r-minimal" ,r-minimal)
       ("r-foreach" ,r-foreach)
       ("r-xnomial" ,r-xnomial)
       ("r-domc" ,r-domc)
       ("r-multitaper" ,r-multitaper)
       ("r-seqinr" ,r-seqinr)))
    (home-page "https://ohlerlab.mdc-berlin.de/software/RiboTaper_126/")
    (synopsis "Define translated ORFs using ribosome profiling data")
    (description
     "Ribotaper is a method for defining translated @dfn{open reading
frames} (ORFs) using ribosome profiling (ribo-seq) data.  This package
provides the Ribotaper pipeline.")
    (license license:gpl3+)))

(define-public ribodiff
  (package
    (name "ribodiff")
    (version "0.2.2")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/ratschlab/RiboDiff")
             (commit (string-append "v" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "0x75nlp7qnmm64jasbi6l21f2cy99r2cjyl6b4hr8zf2bq22drnz"))))
    (build-system python-build-system)
    (arguments
     `(#:python ,python-2
       #:phases
       (modify-phases %standard-phases
         ;; Generate an installable executable script wrapper.
         (add-after 'unpack 'patch-setup.py
           (lambda _
             (substitute* "setup.py"
               (("^(.*)packages=.*" line prefix)
                (string-append line "\n"
                               prefix "scripts=['scripts/TE.py'],\n")))
             #t)))))
    (inputs
     `(("python-numpy" ,python2-numpy)
       ("python-matplotlib" ,python2-matplotlib)
       ("python-scipy" ,python2-scipy)
       ("python-statsmodels" ,python2-statsmodels)))
    (native-inputs
     `(("python-mock" ,python2-mock)
       ("python-nose" ,python2-nose)))
    (home-page "https://public.bmi.inf.ethz.ch/user/zhongy/RiboDiff/")
    (synopsis "Detect translation efficiency changes from ribosome footprints")
    (description "RiboDiff is a statistical tool that detects the protein
translational efficiency change from Ribo-Seq (ribosome footprinting) and
RNA-Seq data.  It uses a generalized linear model to detect genes showing
difference in translational profile taking mRNA abundance into account.  It
facilitates us to decipher the translational regulation that behave
independently with transcriptional regulation.")
    (license license:gpl3+)))

(define-public bioawk
  (package
    (name "bioawk")
    (version "1.0")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/lh3/bioawk")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1pxc3zdnirxbf9a0az698hd8xdik7qkhypm7v6hn922x8y9qmspm"))))
    (build-system gnu-build-system)
    (inputs
     `(("zlib" ,zlib)))
    (native-inputs
     `(("bison" ,bison)))
    (arguments
     `(#:tests? #f ; There are no tests to run.
       ;; Bison must generate files, before other targets can build.
       #:parallel-build? #f
       #:phases
       (modify-phases %standard-phases
         (delete 'configure) ; There is no configure phase.
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin  (string-append out "/bin"))
                    (man (string-append out "/share/man/man1")))
               (mkdir-p man)
               (copy-file "awk.1" (string-append man "/bioawk.1"))
               (install-file "bioawk" bin))
             #t)))))
    (home-page "https://github.com/lh3/bioawk")
    (synopsis "AWK with bioinformatics extensions")
    (description "Bioawk is an extension to Brian Kernighan's awk, adding the
support of several common biological data formats, including optionally gzip'ed
BED, GFF, SAM, VCF, FASTA/Q and TAB-delimited formats with column names.  It
also adds a few built-in functions and a command line option to use TAB as the
input/output delimiter.  When the new functionality is not used, bioawk is
intended to behave exactly the same as the original BWK awk.")
    (license license:x11)))

(define-public python-pybedtools
  (package
    (name "python-pybedtools")
    (version "0.8.1")
    (source (origin
              (method url-fetch)
              (uri (pypi-uri "pybedtools" version))
              (sha256
               (base32
                "14w5i40gi25clrr7h4wa2pcpnyipya8hrqi7nq77553zc5wf0df0"))))
    (build-system python-build-system)
    (arguments
     `(#:modules ((ice-9 ftw)
                  (srfi srfi-1)
                  (srfi srfi-26)
                  (guix build utils)
                  (guix build python-build-system))
       ;; See https://github.com/daler/pybedtools/issues/192
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'disable-broken-tests
           (lambda _
             (substitute* "pybedtools/test/test_scripts.py"
               ;; This test freezes.
               (("def test_intron_exon_reads")
                "def _do_not_test_intron_exon_reads")
               ;; This test fails in the Python 2 build.
               (("def test_venn_mpl")
                "def _do_not_test_venn_mpl"))
             (substitute* "pybedtools/test/test_helpers.py"
               ;; Requires internet access.
               (("def test_chromsizes")
                "def _do_not_test_chromsizes")
               ;; Broken as a result of the workaround used in the check phase
               ;; (see: https://github.com/daler/pybedtools/issues/192).
               (("def test_getting_example_beds")
                "def _do_not_test_getting_example_beds"))
             ;; This issue still occurs on python2
             (substitute* "pybedtools/test/test_issues.py"
               (("def test_issue_303")
                "def _test_issue_303"))
             #t))
         ;; TODO: Remove phase after it's part of PYTHON-BUILD-SYSTEM.
         ;; build system.
         ;; Force the Cythonization of C++ files to guard against compilation
         ;; problems.
         (add-after 'unpack 'remove-cython-generated-files
           (lambda _
             (let ((cython-sources (map (cut string-drop-right <> 4)
                                        (find-files "." "\\.pyx$")))
                   (c/c++-files (find-files "." "\\.(c|cpp|cxx)$")))
               (define (strip-extension filename)
                 (string-take filename (string-index-right filename #\.)))
               (define (cythonized? c/c++-file)
                 (member (strip-extension c/c++-file) cython-sources))
               (for-each delete-file (filter cythonized? c/c++-files))
               #t)))
         (add-after 'remove-cython-generated-files 'generate-cython-extensions
           (lambda _
             (invoke "python" "setup.py" "cythonize")))
         (replace 'check
           (lambda _
             (let* ((cwd (getcwd))
                    (build-root-directory (string-append cwd "/build/"))
                    (build (string-append
                            build-root-directory
                            (find (cut string-prefix? "lib" <>)
                                  (scandir (string-append
                                            build-root-directory)))))
                    (scripts (string-append
                              build-root-directory
                              (find (cut string-prefix? "scripts" <>)
                                    (scandir build-root-directory)))))
               (setenv "PYTHONPATH"
                       (string-append build ":" (getenv "PYTHONPATH")))
               ;; Executable scripts such as 'intron_exon_reads.py' must be
               ;; available in the PATH.
               (setenv "PATH"
                       (string-append scripts ":" (getenv "PATH"))))
             ;; The tests need to be run from elsewhere...
             (mkdir-p "/tmp/test")
             (copy-recursively "pybedtools/test" "/tmp/test")
             (with-directory-excursion "/tmp/test"
               (invoke "pytest" "-v" "--doctest-modules")))))))
    (propagated-inputs
     `(("bedtools" ,bedtools)
       ("samtools" ,samtools)
       ("python-matplotlib" ,python-matplotlib)
       ("python-pysam" ,python-pysam)
       ("python-pyyaml" ,python-pyyaml)))
    (native-inputs
     `(("python-numpy" ,python-numpy)
       ("python-pandas" ,python-pandas)
       ("python-cython" ,python-cython)
       ("kentutils" ,kentutils)         ; for bedGraphToBigWig
       ("python-six" ,python-six)
       ;; For the test suite.
       ("python-pytest" ,python-pytest)
       ("python-psutil" ,python-psutil)))
    (home-page "https://pythonhosted.org/pybedtools/")
    (synopsis "Python wrapper for BEDtools programs")
    (description
     "pybedtools is a Python wrapper for Aaron Quinlan's BEDtools programs,
which are widely used for genomic interval manipulation or \"genome algebra\".
pybedtools extends BEDTools by offering feature-level manipulations from with
Python.")
    (license license:gpl2+)))

(define-public python2-pybedtools
  (let ((pybedtools (package-with-python2 python-pybedtools)))
    (package
      (inherit pybedtools)
      (native-inputs
       `(("python2-pathlib" ,python2-pathlib)
         ,@(package-native-inputs pybedtools))))))

(define-public python-biom-format
  (package
    (name "python-biom-format")
    (version "2.1.7")
    (source
     (origin
       (method git-fetch)
       ;; Use GitHub as source because PyPI distribution does not contain
       ;; test data: https://github.com/biocore/biom-format/issues/693
       (uri (git-reference
             (url "https://github.com/biocore/biom-format")
             (commit version)))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "1rna16lyk5aqhnv0dp77wwaplias93f1vw28ad3jmyw6hwkai05v"))
       (modules '((guix build utils)))
       (snippet '(begin
                   ;; Delete generated C files.
                   (for-each delete-file (find-files "." "\\.c"))
                   #t))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'use-cython
           (lambda _ (setenv "USE_CYTHON" "1") #t))
         (add-after 'unpack 'disable-broken-tests
           (lambda _
             (substitute* "biom/tests/test_cli/test_validate_table.py"
               (("^(.+)def test_invalid_hdf5" m indent)
                (string-append indent
                               "@npt.dec.skipif(True, msg='Guix')\n"
                               m)))
             (substitute* "biom/tests/test_table.py"
               (("^(.+)def test_from_hdf5_issue_731" m indent)
                (string-append indent
                               "@npt.dec.skipif(True, msg='Guix')\n"
                               m)))
             #t))
         (add-before 'reset-gzip-timestamps 'make-files-writable
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((out (assoc-ref outputs "out")))
               (for-each (lambda (file) (chmod file #o644))
                         (find-files out "\\.gz"))
               #t))))))
    (propagated-inputs
     `(("python-numpy" ,python-numpy)
       ("python-scipy" ,python-scipy)
       ("python-flake8" ,python-flake8)
       ("python-future" ,python-future)
       ("python-click" ,python-click)
       ("python-h5py" ,python-h5py)
       ;; FIXME: Upgrade to pandas 1.0 when
       ;; https://github.com/biocore/biom-format/issues/837 is resolved.
       ("python-pandas" ,python-pandas-0.25)))
    (native-inputs
     `(("python-cython" ,python-cython)
       ("python-pytest" ,python-pytest)
       ("python-pytest-cov" ,python-pytest-cov)
       ("python-nose" ,python-nose)))
    (home-page "http://www.biom-format.org")
    (synopsis "Biological Observation Matrix (BIOM) format utilities")
    (description
     "The BIOM file format is designed to be a general-use format for
representing counts of observations e.g. operational taxonomic units, KEGG
orthology groups or lipid types, in one or more biological samples
e.g. microbiome samples, genomes, metagenomes.")
    (license license:bsd-3)
    (properties `((python2-variant . ,(delay python2-biom-format))))))

(define-public python2-biom-format
  (let ((base (package-with-python2 (strip-python2-variant python-biom-format))))
    (package
      (inherit base)
      (arguments
       (substitute-keyword-arguments (package-arguments base)
         ((#:phases phases)
          `(modify-phases ,phases
             ;; Do not require the unmaintained pyqi library.
             (add-after 'unpack 'remove-pyqi
               (lambda _
                 (substitute* "setup.py"
                   (("install_requires.append\\(\"pyqi\"\\)") "pass"))
                 #t)))))))))

(define-public python-pairtools
  (package
    (name "python-pairtools")
    (version "0.3.0")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/mirnylab/pairtools")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "0gr8y13q7sd6yai6df4aavl2470n1f9s3cib6r473z4hr8hcbwmc"))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'fix-references
           (lambda _
             (substitute* '("pairtools/pairtools_merge.py"
                            "pairtools/pairtools_sort.py")
               (("/bin/bash") (which "bash")))
             #t))
         (replace 'check
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (add-installed-pythonpath inputs outputs)
             (with-directory-excursion "/tmp"
               (invoke "pytest" "-v")))))))
    (native-inputs
     `(("python-cython" ,python-cython)
       ("python-nose" ,python-nose)
       ("python-pytest" ,python-pytest)))
    (inputs
     `(("python" ,python-wrapper)))
    (propagated-inputs
     `(("htslib" ,htslib)               ; for bgzip, looked up in PATH
       ("samtools" ,samtools)           ; looked up in PATH
       ("lz4" ,lz4) ; for lz4c
       ("python-click" ,python-click)
       ("python-numpy" ,python-numpy)))
    (home-page "https://github.com/mirnylab/pairtools")
    (synopsis "Process mapped Hi-C data")
    (description "Pairtools is a simple and fast command-line framework to
process sequencing data from a Hi-C experiment.  Process pair-end sequence
alignments and perform the following operations:

@itemize
@item detect ligation junctions (a.k.a. Hi-C pairs) in aligned paired-end
  sequences of Hi-C DNA molecules
@item sort @code{.pairs} files for downstream analyses
@item detect, tag and remove PCR/optical duplicates
@item generate extensive statistics of Hi-C datasets
@item select Hi-C pairs given flexibly defined criteria
@item restore @code{.sam} alignments from Hi-C pairs.
@end itemize
")
    (license license:expat)))

(define-public bioperl-minimal
  (let* ((inputs `(("perl-module-build" ,perl-module-build)
                   ("perl-data-stag" ,perl-data-stag)
                   ("perl-libwww" ,perl-libwww)
                   ("perl-uri" ,perl-uri)))
         (transitive-inputs
          (map (compose package-name cadr)
               (delete-duplicates
                (concatenate
                 (map (compose package-transitive-target-inputs cadr) inputs))))))
    (package
      (name "bioperl-minimal")
      (version "1.7.0")
      (source
       (origin
         (method git-fetch)
         (uri (git-reference
                (url "https://github.com/bioperl/bioperl-live")
                (commit (string-append "release-"
                                       (string-map (lambda (c)
                                                     (if (char=? c #\.)
                                                         #\- c)) version)))))
         (file-name (git-file-name name version))
         (sha256
          (base32
           "0wl8yvzcls59pwwk6m8ahy87pwg6nnibzy5cldbvmcwg2x2w7783"))))
      (build-system perl-build-system)
      (arguments
       `(#:phases
         (modify-phases %standard-phases
           (add-after
            'install 'wrap-programs
            (lambda* (#:key outputs #:allow-other-keys)
              ;; Make sure all executables in "bin" find the required Perl
              ;; modules at runtime.  As the PERL5LIB variable contains also
              ;; the paths of native inputs, we pick the transitive target
              ;; inputs from %build-inputs.
              (let* ((out  (assoc-ref outputs "out"))
                     (bin  (string-append out "/bin/"))
                     (path (string-join
                            (cons (string-append out "/lib/perl5/site_perl")
                                  (map (lambda (name)
                                         (assoc-ref %build-inputs name))
                                       ',transitive-inputs))
                            ":")))
                (for-each (lambda (file)
                            (wrap-program file
                              `("PERL5LIB" ":" prefix (,path))))
                          (find-files bin "\\.pl$"))
                #t))))))
      (inputs inputs)
      (native-inputs
       `(("perl-test-most" ,perl-test-most)))
      (home-page "https://metacpan.org/release/BioPerl")
      (synopsis "Bioinformatics toolkit")
      (description
       "BioPerl is the product of a community effort to produce Perl code which
is useful in biology.  Examples include Sequence objects, Alignment objects
and database searching objects.  These objects not only do what they are
advertised to do in the documentation, but they also interact - Alignment
objects are made from the Sequence objects, Sequence objects have access to
Annotation and SeqFeature objects and databases, Blast objects can be
converted to Alignment objects, and so on.  This means that the objects
provide a coordinated and extensible framework to do computational biology.")
      (license license:perl-license))))

(define-public python-biopython
  (package
    (name "python-biopython")
    (version "1.70")
    (source (origin
              (method url-fetch)
              ;; use PyPi rather than biopython.org to ease updating
              (uri (pypi-uri "biopython" version))
              (sha256
               (base32
                "0nz4n9d2y2dg849gn1z0vjlkwcpzzkzy3fij7x94a6ixy2c54z2a"))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-before 'check 'set-home
           ;; Some tests require a home directory to be set.
           (lambda _ (setenv "HOME" "/tmp") #t)))))
    (propagated-inputs
     `(("python-numpy" ,python-numpy)))
    (home-page "https://biopython.org/")
    (synopsis "Tools for biological computation in Python")
    (description
     "Biopython is a set of tools for biological computation including parsers
for bioinformatics files into Python data structures; interfaces to common
bioinformatics programs; a standard sequence class and tools for performing
common operations on them; code to perform data classification; code for
dealing with alignments; code making it easy to split up parallelizable tasks
into separate processes; and more.")
    (license (license:non-copyleft "http://www.biopython.org/DIST/LICENSE"))))

(define-public python2-biopython
  (package-with-python2 python-biopython))

(define-public python-fastalite
  (package
    (name "python-fastalite")
    (version "0.3")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "fastalite" version))
       (sha256
        (base32
         "1qli6pxp77i9xn2wfciq2zaxhl82bdxb33cpzqzj1z25yd036wqj"))))
    (build-system python-build-system)
    (arguments
     `(#:tests? #f)) ; Test data is not distributed.
    (home-page "https://github.com/nhoffman/fastalite")
    (synopsis "Simplest possible FASTA parser")
    (description "This library implements a FASTA and a FASTQ parser without
relying on a complex dependency tree.")
    (license license:expat)))

(define-public python2-fastalite
  (package-with-python2 python-fastalite))

(define-public bpp-core
  ;; The last release was in 2014 and the recommended way to install from source
  ;; is to clone the git repository, so we do this.
  ;; http://biopp.univ-montp2.fr/wiki/index.php/Main_Page
  (let ((commit "7d8bced0d1a87291ea8dd7046b7fb5ff9c35c582"))
    (package
      (name "bpp-core")
      (version (string-append "2.2.0-1." (string-take commit 7)))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "http://biopp.univ-montp2.fr/git/bpp-core")
                      (commit commit)))
                (file-name (string-append name "-" version "-checkout"))
                (sha256
                 (base32
                  "10djsq5vlnkilv436gnmh4irpk49v29pa69r6xiryg32xmvn909j"))))
      (build-system cmake-build-system)
      (arguments
       `(#:parallel-build? #f))
      (home-page "http://biopp.univ-montp2.fr")
      (synopsis "C++ libraries for Bioinformatics")
      (description
       "Bio++ is a set of C++ libraries for Bioinformatics, including sequence
analysis, phylogenetics, molecular evolution and population genetics.  It is
Object Oriented and is designed to be both easy to use and computer efficient.
Bio++ intends to help programmers to write computer expensive programs, by
providing them a set of re-usable tools.")
      (license license:cecill-c))))

(define-public bpp-phyl
  ;; The last release was in 2014 and the recommended way to install from source
  ;; is to clone the git repository, so we do this.
  ;; http://biopp.univ-montp2.fr/wiki/index.php/Main_Page
  (let ((commit "0c07167b629f68b569bf274d1ad0c4af83276ae2"))
    (package
      (name "bpp-phyl")
      (version (string-append "2.2.0-1." (string-take commit 7)))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "http://biopp.univ-montp2.fr/git/bpp-phyl")
                      (commit commit)))
                (file-name (string-append name "-" version "-checkout"))
                (sha256
                 (base32
                  "1ssjgchzwj3iai26kyly7gwkdv8sk59nqhkb1wpap3sf5m6kyllh"))))
      (build-system cmake-build-system)
      (arguments
       `(#:parallel-build? #f
         ;; If out-of-source, test data is not copied into the build directory
         ;; so the tests fail.
         #:out-of-source? #f))
      (inputs
       `(("bpp-core" ,bpp-core)
         ("bpp-seq" ,bpp-seq)))
      (home-page "http://biopp.univ-montp2.fr")
      (synopsis "Bio++ phylogenetic Library")
      (description
       "Bio++ is a set of C++ libraries for Bioinformatics, including sequence
analysis, phylogenetics, molecular evolution and population genetics.  This
library provides phylogenetics-related modules.")
      (license license:cecill-c))))

(define-public bpp-popgen
  ;; The last release was in 2014 and the recommended way to install from source
  ;; is to clone the git repository, so we do this.
  ;; http://biopp.univ-montp2.fr/wiki/index.php/Main_Page
  (let ((commit "e472bac9b1a148803895d747cd6d0c5904f85d9f"))
    (package
      (name "bpp-popgen")
      (version (string-append "2.2.0-1." (string-take commit 7)))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "http://biopp.univ-montp2.fr/git/bpp-popgen")
                      (commit commit)))
                (file-name (string-append name "-" version "-checkout"))
                (sha256
                 (base32
                  "0yn82dzn1n5629nzja68xfrhi655709rjanyryb36vzkmymy6dw5"))))
      (build-system cmake-build-system)
      (arguments
       `(#:parallel-build? #f
         #:tests? #f)) ; There are no tests.
      (inputs
       `(("bpp-core" ,bpp-core)
         ("bpp-seq" ,bpp-seq)))
      (home-page "http://biopp.univ-montp2.fr")
      (synopsis "Bio++ population genetics library")
      (description
       "Bio++ is a set of C++ libraries for Bioinformatics, including sequence
analysis, phylogenetics, molecular evolution and population genetics.  This
library provides population genetics-related modules.")
      (license license:cecill-c))))

(define-public bpp-seq
  ;; The last release was in 2014 and the recommended way to install from source
  ;; is to clone the git repository, so we do this.
  ;; http://biopp.univ-montp2.fr/wiki/index.php/Main_Page
  (let ((commit "6cfa07965ce152e5598a89df2fa80a75973bfa33"))
    (package
      (name "bpp-seq")
      (version (string-append "2.2.0-1." (string-take commit 7)))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "http://biopp.univ-montp2.fr/git/bpp-seq")
                      (commit commit)))
                (file-name (string-append name "-" version "-checkout"))
                (sha256
                 (base32
                  "1nys5jq7jqvdg40d91wsmj3q2yzy4276cp7sp44n67p468f27zf2"))))
      (build-system cmake-build-system)
      (arguments
       `(#:parallel-build? #f
         ;; If out-of-source, test data is not copied into the build directory
         ;; so the tests fail.
         #:out-of-source? #f))
      (inputs
       `(("bpp-core" ,bpp-core)))
      (home-page "http://biopp.univ-montp2.fr")
      (synopsis "Bio++ sequence library")
      (description
       "Bio++ is a set of C++ libraries for Bioinformatics, including sequence
analysis, phylogenetics, molecular evolution and population genetics.  This
library provides sequence-related modules.")
      (license license:cecill-c))))

(define-public bppsuite
  ;; The last release was in 2014 and the recommended way to install from source
  ;; is to clone the git repository, so we do this.
  ;; http://biopp.univ-montp2.fr/wiki/index.php/Main_Page
  (let ((commit "c516147f57aa50961121cd505bed52cd7603698b"))
    (package
      (name "bppsuite")
      (version (string-append "2.2.0-1." (string-take commit 7)))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "http://biopp.univ-montp2.fr/git/bppsuite")
                      (commit commit)))
                (file-name (string-append name "-" version "-checkout"))
                (sha256
                 (base32
                  "1y87pxvw0jxjizhq2dr9g2r91md45k1p9ih2sl1yy1y3p934l2kb"))))
      (build-system cmake-build-system)
      (arguments
       `(#:parallel-build? #f
         #:tests? #f)) ; There are no tests.
      (native-inputs
       `(("groff" ,groff)
         ("man-db" ,man-db)
         ("texinfo" ,texinfo)))
      (inputs
       `(("bpp-core" ,bpp-core)
         ("bpp-seq" ,bpp-seq)
         ("bpp-phyl" ,bpp-phyl)
         ("bpp-phyl" ,bpp-popgen)))
      (home-page "http://biopp.univ-montp2.fr")
      (synopsis "Bioinformatics tools written with the Bio++ libraries")
      (description
       "Bio++ is a set of C++ libraries for Bioinformatics, including sequence
analysis, phylogenetics, molecular evolution and population genetics.  This
package provides command line tools using the Bio++ library.")
      (license license:cecill-c))))

(define-public blast+
  (package
    (name "blast+")
    (version "2.10.1")
    (source (origin
              (method url-fetch)
              (uri (string-append
                    "https://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/"
                    version "/ncbi-blast-" version "+-src.tar.gz"))
              (sha256
               (base32
                "11kvrrl0mcwww6530r55hccpg3x3msmhr3051fwnjbq8rzg2j1qi"))
              (modules '((guix build utils)))
              (snippet
               '(begin
                  ;; Remove bundled bzip2, zlib and pcre.
                  (delete-file-recursively "c++/src/util/compress/bzip2")
                  (delete-file-recursively "c++/src/util/compress/zlib")
                  (delete-file-recursively "c++/src/util/regexp")
                  (substitute* "c++/src/util/compress/Makefile.in"
                    (("bzip2 zlib api") "api"))
                  ;; Remove useless msbuild directory
                  (delete-file-recursively
                   "c++/src/build-system/project_tree_builder/msbuild")
                  #t))))
    (build-system gnu-build-system)
    (arguments
     `(;; There are two(!) tests for this massive library, and both fail with
       ;; "unparsable timing stats".
       ;; ERR [127] --  [serial/datatool] datatool.sh     (unparsable timing stats)
       ;; ERR [127] --  [serial/datatool] datatool_xml.sh     (unparsable timing stats)
       #:tests? #f
       #:out-of-source? #t
       #:parallel-build? #f ; not supported
       #:phases
       (modify-phases %standard-phases
         (add-before 'configure 'set-HOME
          ;; $HOME needs to be set at some point during the configure phase
          (lambda _ (setenv "HOME" "/tmp") #t))
         (add-after 'unpack 'enter-dir
          (lambda _ (chdir "c++") #t))
         (add-after 'enter-dir 'fix-build-system
          (lambda _
            (define (which* cmd)
              (cond ((string=? cmd "date")
                     ;; make call to "date" deterministic
                     "date -d @0")
                    ((which cmd)
                     => identity)
                    (else
                     (format (current-error-port)
                             "WARNING: Unable to find absolute path for ~s~%"
                             cmd)
                     #f)))

            ;; Rewrite hardcoded paths to various tools
            (substitute* (append '("src/build-system/configure.ac"
                                   "src/build-system/configure"
                                   "src/build-system/helpers/run_with_lock.c"
                                   "scripts/common/impl/if_diff.sh"
                                   "scripts/common/impl/run_with_lock.sh"
                                   "src/build-system/Makefile.configurables.real"
                                   "src/build-system/Makefile.in.top"
                                   "src/build-system/Makefile.meta.gmake=no"
                                   "src/build-system/Makefile.meta.in"
                                   "src/build-system/Makefile.meta_l"
                                   "src/build-system/Makefile.meta_p"
                                   "src/build-system/Makefile.meta_r"
                                   "src/build-system/Makefile.mk.in"
                                   "src/build-system/Makefile.requirements"
                                   "src/build-system/Makefile.rules_with_autodep.in")
                                 (find-files "scripts/common/check" "\\.sh$"))
              (("(/usr/bin/|/bin/)([a-z][-_.a-z]*)" all dir cmd)
               (or (which* cmd) all)))

            (substitute* (find-files "src/build-system" "^config.*")
              (("LN_S=/bin/\\$LN_S") (string-append "LN_S=" (which "ln")))
              (("^PATH=.*") ""))

            ;; rewrite "/var/tmp" in check script
            (substitute* "scripts/common/check/check_make_unix.sh"
              (("/var/tmp") "/tmp"))

            ;; do not reset PATH
            (substitute* (find-files "scripts/common/impl/" "\\.sh$")
              (("^ *PATH=.*") "")
              (("action=/bin/") "action=")
              (("export PATH") ":"))
            #t))
         (replace 'configure
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let ((out     (assoc-ref outputs "out"))
                   (lib     (string-append (assoc-ref outputs "lib") "/lib"))
                   (include (string-append (assoc-ref outputs "include")
                                           "/include/ncbi-tools++")))
               ;; The 'configure' script doesn't recognize things like
               ;; '--enable-fast-install'.
               (invoke "./configure.orig"
                       (string-append "--with-build-root=" (getcwd) "/build")
                       (string-append "--prefix=" out)
                       (string-append "--libdir=" lib)
                       (string-append "--includedir=" include)
                       (string-append "--with-bz2="
                                      (assoc-ref inputs "bzip2"))
                       (string-append "--with-z="
                                      (assoc-ref inputs "zlib"))
                       (string-append "--with-pcre="
                                      (assoc-ref inputs "pcre"))
                       ;; Each library is built twice by default, once
                       ;; with "-static" in its name, and again
                       ;; without.
                       "--without-static"
                       "--with-dll")
               #t))))))
    (outputs '("out"       ;  21 MB
               "lib"       ; 226 MB
               "include")) ;  33 MB
    (inputs
     `(("bzip2" ,bzip2)
       ("lmdb" ,lmdb)
       ("zlib" ,zlib)
       ("pcre" ,pcre)
       ("perl" ,perl)
       ("python" ,python-wrapper)))
    (native-inputs
     `(("cpio" ,cpio)))
    (home-page "https://blast.ncbi.nlm.nih.gov")
    (synopsis "Basic local alignment search tool")
    (description
     "BLAST is a popular method of performing a DNA or protein sequence
similarity search, using heuristics to produce results quickly.  It also
calculates an “expect value” that estimates how many matches would have
occurred at a given score by chance, which can aid a user in judging how much
confidence to have in an alignment.")
    ;; Most of the sources are in the public domain, with the following
    ;; exceptions:
    ;;   * Expat:
    ;;     * ./c++/include/util/bitset/
    ;;     * ./c++/src/html/ncbi_menu*.js
    ;;   * Boost license:
    ;;     * ./c++/include/util/impl/floating_point_comparison.hpp
    ;;   * LGPL 2+:
    ;;     * ./c++/include/dbapi/driver/odbc/unix_odbc/
    ;;   * ASL 2.0:
    ;;     * ./c++/src/corelib/teamcity_*
    (license (list license:public-domain
                   license:expat
                   license:boost1.0
                   license:lgpl2.0+
                   license:asl2.0))))

(define-public bless
  (package
    (name "bless")
    (version "1p02")
    (source (origin
              (method url-fetch)
              (uri (string-append "mirror://sourceforge/bless-ec/bless.v"
                                  version ".tgz"))
              (sha256
               (base32
                "0rm0gw2s18dqwzzpl3c2x1z05ni2v0xz5dmfk3d33j6g4cgrlrdd"))
              (modules '((guix build utils)))
              (snippet
               `(begin
                  ;; Remove bundled boost, pigz, zlib, and .git directory
                  ;; FIXME: also remove bundled sources for murmurhash3 and
                  ;; kmc once packaged.
                  (delete-file-recursively "boost")
                  (delete-file-recursively "pigz")
                  (delete-file-recursively "google-sparsehash")
                  (delete-file-recursively "zlib")
                  (delete-file-recursively ".git")
                  #t))))
    (build-system gnu-build-system)
    (arguments
     '(#:tests? #f ;no "check" target
       #:make-flags
       (list (string-append "ZLIB="
                            (assoc-ref %build-inputs "zlib:static")
                            "/lib/libz.a")
             (string-append "LDFLAGS="
                            (string-join '("-lboost_filesystem"
                                           "-lboost_system"
                                           "-lboost_iostreams"
                                           "-lz"
                                           "-fopenmp"))))
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'do-not-build-bundled-pigz
          (lambda* (#:key inputs outputs #:allow-other-keys)
            (substitute* "Makefile"
              (("cd pigz/pigz-2.3.3; make") ""))
            #t))
         (add-after 'unpack 'patch-paths-to-executables
          (lambda* (#:key inputs outputs #:allow-other-keys)
            (substitute* "parse_args.cpp"
              (("kmc_binary = .*")
               (string-append "kmc_binary = \""
                              (assoc-ref outputs "out")
                              "/bin/kmc\";"))
              (("pigz_binary = .*")
               (string-append "pigz_binary = \""
                              (assoc-ref inputs "pigz")
                              "/bin/pigz\";")))
            #t))
         (replace 'install
          (lambda* (#:key outputs #:allow-other-keys)
            (let ((bin (string-append (assoc-ref outputs "out") "/bin/")))
              (for-each (lambda (file)
                          (install-file file bin))
                        '("bless" "kmc/bin/kmc"))
              #t)))
         (delete 'configure))))
    (native-inputs
     `(("perl" ,perl)))
    (inputs
     `(("openmpi" ,openmpi)
       ("boost" ,boost)
       ("sparsehash" ,sparsehash)
       ("pigz" ,pigz)
       ("zlib:static" ,zlib "static")
       ("zlib" ,zlib)))
    (supported-systems '("x86_64-linux"))
    (home-page "https://sourceforge.net/p/bless-ec/wiki/Home/")
    (synopsis "Bloom-filter-based error correction tool for NGS reads")
    (description
     "@dfn{Bloom-filter-based error correction solution for high-throughput
sequencing reads} (BLESS) uses a single minimum-sized bloom filter is a
correction tool for genomic reads produced by @dfn{Next-generation
sequencing} (NGS).  BLESS produces accurate correction results with much less
memory compared with previous solutions and is also able to tolerate a higher
false-positive rate.  BLESS can extend reads like DNA assemblers to correct
errors at the end of reads.")
    (license license:gpl3+)))

(define-public bowtie
  (package
    (name "bowtie")
    (version "2.3.4.3")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/BenLangmead/bowtie2")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1zl3cf327y2p7p03cavymbh7b00djc7lncfaqih33n96iy9q8ibp"))
              (modules '((guix build utils)))
              (snippet
               '(begin
                  (substitute* "Makefile"
                    ;; replace BUILD_HOST and BUILD_TIME for deterministic build
                    (("-DBUILD_HOST=.*") "-DBUILD_HOST=\"\\\"guix\\\"\"")
                    (("-DBUILD_TIME=.*") "-DBUILD_TIME=\"\\\"0\\\"\""))
                  #t))))
    (build-system gnu-build-system)
    (arguments
     '(#:make-flags
       (list "allall"
             "WITH_TBB=1"
             (string-append "prefix=" (assoc-ref %outputs "out")))
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (replace 'check
           (lambda _
             (invoke "perl"
                     "scripts/test/simple_tests.pl"
                     "--bowtie2=./bowtie2"
                     "--bowtie2-build=./bowtie2-build")
             #t)))))
    (inputs
     `(("tbb" ,tbb)
       ("zlib" ,zlib)
       ("python" ,python-wrapper)))
    (native-inputs
     `(("perl" ,perl)
       ("perl-clone" ,perl-clone)
       ("perl-test-deep" ,perl-test-deep)
       ("perl-test-simple" ,perl-test-simple)))
    (home-page "http://bowtie-bio.sourceforge.net/bowtie2/index.shtml")
    (synopsis "Fast and sensitive nucleotide sequence read aligner")
    (description
     "Bowtie 2 is a fast and memory-efficient tool for aligning sequencing
reads to long reference sequences.  It is particularly good at aligning reads
of about 50 up to 100s or 1,000s of characters, and particularly good at
aligning to relatively long (e.g. mammalian) genomes.  Bowtie 2 indexes the
genome with an FM Index to keep its memory footprint small: for the human
genome, its memory footprint is typically around 3.2 GB.  Bowtie 2 supports
gapped, local, and paired-end alignment modes.")
    (supported-systems '("x86_64-linux"))
    (license license:gpl3+)))

(define-public bowtie1
  (package
    (name "bowtie1")
    (version "1.2.3")
    (source (origin
              (method url-fetch)
              (uri (string-append "mirror://sourceforge/bowtie-bio/bowtie/"
                                  version "/bowtie-src-x86_64.zip"))
              (sha256
               (base32
                "0vmiqdhc9dzyfy9sh6vgi7k9xy2hiw8g87vbamnc6cgpm179zsa4"))
              (modules '((guix build utils)))
              (snippet
               '(substitute* "Makefile"
                  ;; replace BUILD_HOST and BUILD_TIME for deterministic build
                  (("-DBUILD_HOST=.*") "-DBUILD_HOST=\"\\\"guix\\\"\"")
                  (("-DBUILD_TIME=.*") "-DBUILD_TIME=\"\\\"0\\\"\"")))))
    (build-system gnu-build-system)
    (arguments
     '(#:tests? #f                      ; no "check" target
       #:make-flags
       (list "all"
             (string-append "prefix=" (assoc-ref %outputs "out")))
       #:phases
       (modify-phases %standard-phases
         (delete 'configure))))
    (inputs
     `(("python-wrapper" ,python-wrapper)
       ("tbb" ,tbb)
       ("zlib" ,zlib)))
    (supported-systems '("x86_64-linux"))
    (home-page "http://bowtie-bio.sourceforge.net/index.shtml")
    (synopsis "Fast aligner for short nucleotide sequence reads")
    (description
     "Bowtie is a fast, memory-efficient short read aligner.  It aligns short
DNA sequences (reads) to the human genome at a rate of over 25 million 35-bp
reads per hour.  Bowtie indexes the genome with a Burrows-Wheeler index to
keep its memory footprint small: typically about 2.2 GB for the human
genome (2.9 GB for paired-end).")
    (license license:artistic2.0)))

(define-public tophat
  (package
    (name "tophat")
    (version "2.1.1")
    (source (origin
              (method url-fetch)
              (uri (string-append
                    "http://ccb.jhu.edu/software/tophat/downloads/tophat-"
                    version ".tar.gz"))
              (sha256
               (base32
                "19add02kv2xhd6ihd779dr7x35ggym3jqr0m5c4315i1yfb0p11p"))
              (modules '((guix build utils)))
              (snippet
               '(begin
                  ;; Remove bundled SeqAn and samtools
                  (delete-file-recursively "src/SeqAn-1.4.2")
                  (delete-file-recursively "src/samtools-0.1.18")
                  #t))))
    (build-system gnu-build-system)
    (arguments
     '(#:parallel-build? #f             ; not supported
       #:phases
       (modify-phases %standard-phases
         (add-after 'set-paths 'hide-default-gcc
           (lambda* (#:key inputs #:allow-other-keys)
             (let ((gcc (assoc-ref inputs "gcc")))
               ;; Remove the default GCC from CPLUS_INCLUDE_PATH to prevent
               ;; conflicts with the GCC 5 input.
               (setenv "CPLUS_INCLUDE_PATH"
                       (string-join
                        (delete (string-append gcc "/include/c++")
                                (string-split (getenv "CPLUS_INCLUDE_PATH") #\:))
                        ":"))
               #t)))
         (add-after 'unpack 'use-system-samtools
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* "src/Makefile.in"
               (("(noinst_LIBRARIES = )\\$\\(SAMLIB\\)" _ prefix) prefix)
               (("\\$\\(SAMPROG\\): \\$\\(SAMLIB\\)") "")
               (("SAMPROG = samtools_0\\.1\\.18") "")
               (("\\$\\(samtools_0_1_18_SOURCES\\)") "")
               (("am__EXEEXT_1 = samtools_0\\.1\\.18\\$\\(EXEEXT\\)") ""))
             (substitute* '("src/common.cpp"
                            "src/tophat.py")
               (("samtools_0.1.18") (which "samtools")))
             (substitute* '("src/common.h"
                            "src/bam2fastx.cpp")
               (("#include \"bam.h\"") "#include <samtools/bam.h>")
               (("#include \"sam.h\"") "#include <samtools/sam.h>"))
             (substitute* '("src/bwt_map.h"
                            "src/map2gtf.h"
                            "src/align_status.h")
               (("#include <bam.h>") "#include <samtools/bam.h>")
               (("#include <sam.h>") "#include <samtools/sam.h>"))
             #t)))))
    (native-inputs
     `(("gcc@5" ,gcc-5))) ;; doesn't build with later versions
    (inputs
     `(("boost" ,boost)
       ("bowtie" ,bowtie)
       ("ncurses" ,ncurses)
       ("perl" ,perl)
       ("python" ,python-2)
       ("samtools" ,samtools-0.1)
       ("seqan" ,seqan-1)
       ("zlib" ,zlib)))
    (home-page "https://ccb.jhu.edu/software/tophat/index.shtml")
    (synopsis "Spliced read mapper for RNA-Seq data")
    (description
     "TopHat is a fast splice junction mapper for nucleotide sequence
reads produced by the RNA-Seq method.  It aligns RNA-Seq reads to
mammalian-sized genomes using the ultra high-throughput short read
aligner Bowtie, and then analyzes the mapping results to identify
splice junctions between exons.")
    ;; TopHat is released under the Boost Software License, Version 1.0
    ;; See https://github.com/infphilo/tophat/issues/11#issuecomment-121589893
    (license license:boost1.0)))

(define-public bwa
  (package
    (name "bwa")
    (version "0.7.17")
    (source (origin
              (method url-fetch)
              (uri (string-append
                    "https://github.com/lh3/bwa/releases/download/v"
                    version "/bwa-" version ".tar.bz2"))
              (sha256
               (base32
                "1zfhv2zg9v1icdlq4p9ssc8k01mca5d1bd87w71py2swfi74s6yy"))))
    (build-system gnu-build-system)
    (arguments
     '(#:tests? #f ;no "check" target
       #:phases
       (modify-phases %standard-phases
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin"))
                    (lib (string-append out "/lib"))
                    (doc (string-append out "/share/doc/bwa"))
                    (man (string-append out "/share/man/man1")))
               (install-file "bwa" bin)
               (install-file "libbwa.a" lib)
               (install-file "README.md" doc)
               (install-file "bwa.1" man))
             #t))
           ;; no "configure" script
          (delete 'configure))))
    (inputs `(("zlib" ,zlib)))
    ;; Non-portable SSE instructions are used so building fails on platforms
    ;; other than x86_64.
    (supported-systems '("x86_64-linux"))
    (home-page "http://bio-bwa.sourceforge.net/")
    (synopsis "Burrows-Wheeler sequence aligner")
    (description
     "BWA is a software package for mapping low-divergent sequences against a
large reference genome, such as the human genome.  It consists of three
algorithms: BWA-backtrack, BWA-SW and BWA-MEM.  The first algorithm is
designed for Illumina sequence reads up to 100bp, while the rest two for
longer sequences ranged from 70bp to 1Mbp.  BWA-MEM and BWA-SW share similar
features such as long-read support and split alignment, but BWA-MEM, which is
the latest, is generally recommended for high-quality queries as it is faster
and more accurate.  BWA-MEM also has better performance than BWA-backtrack for
70-100bp Illumina reads.")
    (license license:gpl3+)))

(define-public bwa-pssm
  (package (inherit bwa)
    (name "bwa-pssm")
    (version "0.5.11")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/pkerpedjiev/bwa-pssm")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "076c4q0cdqz8jgylb067y9zmvxglppnzi3qiscn0xiypgc6lgb5r"))))
    (build-system gnu-build-system)
    (inputs
     `(("gdsl" ,gdsl)
       ("zlib" ,zlib)
       ("perl" ,perl)))
    (home-page "http://bwa-pssm.binf.ku.dk/")
    (synopsis "Burrows-Wheeler transform-based probabilistic short read mapper")
    (description
     "BWA-PSSM is a probabilistic short genomic sequence read aligner based on
the use of @dfn{position specific scoring matrices} (PSSM).  Like many of the
existing aligners it is fast and sensitive.  Unlike most other aligners,
however, it is also adaptible in the sense that one can direct the alignment
based on known biases within the data set.  It is coded as a modification of
the original BWA alignment program and shares the genome index structure as
well as many of the command line options.")
    (license license:gpl3+)))

(define-public bwa-meth
  (package
    (name "bwa-meth")
    (version "0.2.2")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/brentp/bwa-meth")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "17j31i7zws5j7mhsq9x3qgkxly6mlmrgwhfq0qbflgxrmx04yaiz"))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'keep-references-to-bwa
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* "bwameth.py"
               (("bwa (mem|index)" _ command)
                (string-append (which "bwa") " " command))
               ;; There's an ill-advised check for "samtools" on PATH.
               (("^checkX.*") ""))
             #t)))))
    (inputs
     `(("bwa" ,bwa)))
    (native-inputs
     `(("python-toolshed" ,python-toolshed)))
    (home-page "https://github.com/brentp/bwa-meth")
    (synopsis "Fast and accurante alignment of BS-Seq reads")
    (description
     "BWA-Meth works for single-end reads and for paired-end reads from the
directional protocol (most common).  It uses the method employed by
methylcoder and Bismark of in silico conversion of all C's to T's in both
reference and reads.  It recovers the original read (needed to tabulate
methylation) by attaching it as a comment which BWA appends as a tag to the
read.  It performs favorably to existing aligners gauged by number of on and
off-target reads for a capture method that targets CpG-rich region.")
    (license license:expat)))

(define-public python-bx-python
  (package
    (name "python-bx-python")
    (version "0.8.2")
    (source (origin
              (method url-fetch)
              (uri (pypi-uri "bx-python" version))
              (sha256
               (base32
                "11kksg2rbzihpmcid823xvg42xi88m7sz58rzk29abybkxy0rszs"))))
    (build-system python-build-system)
    ;; Tests fail because test data are not included
    (arguments '(#:tests? #f))
    (propagated-inputs
     `(("python-numpy" ,python-numpy)
       ("python-six" ,python-six)))
    (inputs
     `(("zlib" ,zlib)))
    (native-inputs
     `(("python-lzo" ,python-lzo)
       ("python-nose" ,python-nose)
       ("python-cython" ,python-cython)))
    (home-page "https://github.com/bxlab/bx-python")
    (synopsis "Tools for manipulating biological data")
    (description
     "bx-python provides tools for manipulating biological data, particularly
multiple sequence alignments.")
    (license license:expat)))

(define-public python2-bx-python
  (package-with-python2 python-bx-python))

(define-public python-pysam
  (package
    (name "python-pysam")
    (version "0.15.1")
    (source (origin
              (method git-fetch)
              ;; Test data is missing on PyPi.
              (uri (git-reference
                    (url "https://github.com/pysam-developers/pysam")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1vj367w6xbn9bpmksm162l1aipf7cj97h1q83y7jcpm33ihwpf7x"))
              (modules '((guix build utils)))
              (snippet '(begin
                          ;; Drop bundled htslib. TODO: Also remove samtools
                          ;; and bcftools.
                          (delete-file-recursively "htslib")
                          #t))))
    (build-system python-build-system)
    (arguments
     `(#:modules ((ice-9 ftw)
                  (srfi srfi-26)
                  (guix build python-build-system)
                  (guix build utils))
       #:phases
       (modify-phases %standard-phases
         (add-before 'build 'set-flags
           (lambda* (#:key inputs #:allow-other-keys)
             (setenv "HTSLIB_MODE" "external")
             (setenv "HTSLIB_LIBRARY_DIR"
                     (string-append (assoc-ref inputs "htslib") "/lib"))
             (setenv "HTSLIB_INCLUDE_DIR"
                     (string-append (assoc-ref inputs "htslib") "/include"))
             (setenv "LDFLAGS" "-lncurses")
             (setenv "CFLAGS" "-D_CURSES_LIB=1")
             #t))
         (replace 'check
           (lambda* (#:key inputs outputs #:allow-other-keys)
             ;; This file contains tests that require a connection to the
             ;; internet.
             (delete-file "tests/tabix_test.py")
             ;; FIXME: This test fails
             (delete-file "tests/AlignmentFile_test.py")
             ;; Add first subdirectory of "build" directory to PYTHONPATH.
             (setenv "PYTHONPATH"
                     (string-append
                      (getenv "PYTHONPATH")
                      ":" (getcwd) "/build/"
                      (car (scandir "build"
                                    (negate (cut string-prefix? "." <>))))))
             ;; Step out of source dir so python does not import from CWD.
             (with-directory-excursion "tests"
               (setenv "HOME" "/tmp")
               (invoke "make" "-C" "pysam_data")
               (invoke "make" "-C" "cbcf_data")
               ;; Running nosetests without explicitly asking for a single
               ;; process leads to a crash.  Running with multiple processes
               ;; fails because the tests are not designed to run in parallel.

               ;; FIXME: tests keep timing out on some systems.
               (invoke "nosetests" "-v" "--processes" "1")))))))
    (propagated-inputs
     `(("htslib" ,htslib-1.9))) ; Included from installed header files.
    (inputs
     `(("ncurses" ,ncurses)
       ("curl" ,curl)
       ("zlib" ,zlib)))
    (native-inputs
     `(("python-cython" ,python-cython)
       ;; Dependencies below are are for tests only.
       ("samtools" ,samtools-1.9)
       ("bcftools" ,bcftools-1.9)
       ("python-nose" ,python-nose)))
    (home-page "https://github.com/pysam-developers/pysam")
    (synopsis "Python bindings to the SAMtools C API")
    (description
     "Pysam is a Python module for reading and manipulating files in the
SAM/BAM format.  Pysam is a lightweight wrapper of the SAMtools C API.  It
also includes an interface for tabix.")
    (license license:expat)))

(define-public python2-pysam
  (package-with-python2 python-pysam))

(define-public python-twobitreader
  (package
    (name "python-twobitreader")
    (version "3.1.6")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/benjschiller/twobitreader")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1qbxvv1h58cismbk1anpjrkpghsaiy64a11ir3lhy6qch6xf8n62"))))
    (build-system python-build-system)
    ;; Tests are not included
    (arguments '(#:tests? #f))
    (native-inputs
     `(("python-sphinx" ,python-sphinx)))
    (home-page "https://github.com/benjschiller/twobitreader")
    (synopsis "Python library for reading .2bit files")
    (description
     "twobitreader is a Python library for reading .2bit files as used by the
UCSC genome browser.")
    (license license:artistic2.0)))

(define-public python2-twobitreader
  (package-with-python2 python-twobitreader))

(define-public python-plastid
  (package
    (name "python-plastid")
    (version "0.4.8")
    (source (origin
              (method url-fetch)
              (uri (pypi-uri "plastid" version))
              (sha256
               (base32
                "0l24dd3q66if8yj042m4s0g95n6acn7im1imqd3p6h8ns43kxhj8"))))
    (build-system python-build-system)
    (arguments
     ;; Some test files are not included.
     `(#:tests? #f))
    (propagated-inputs
     `(("python-numpy" ,python-numpy)
       ("python-scipy" ,python-scipy)
       ("python-pandas" ,python-pandas)
       ("python-pysam" ,python-pysam)
       ("python-matplotlib" ,python-matplotlib)
       ("python-biopython" ,python-biopython)
       ("python-twobitreader" ,python-twobitreader)
       ("python-termcolor" ,python-termcolor)))
    (native-inputs
     `(("python-cython" ,python-cython)
       ("python-nose" ,python-nose)))
    (home-page "https://github.com/joshuagryphon/plastid")
    (synopsis "Python library for genomic analysis")
    (description
     "plastid is a Python library for genomic analysis – in particular,
high-throughput sequencing data – with an emphasis on simplicity.")
    (license license:bsd-3)))

(define-public python2-plastid
  (package-with-python2 python-plastid))

(define-public tetoolkit
  (package
    (name "tetoolkit")
    (version "2.0.3")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/mhammell-laboratory/tetoolkit")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1yzi0kfpzip8zpjb82x1ik6h22yzfyjiz2dv85v6as2awwqvk807"))))
    (build-system python-build-system)
    (arguments
     `(#:python ,python-2               ; not guaranteed to work with Python 3
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'make-writable
           (lambda _
             (for-each make-file-writable (find-files "."))
             #t))
         (add-after 'unpack 'patch-invocations
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* '("bin/TEtranscripts"
                            "bin/TEcount")
               (("'sort ")
                (string-append "'" (which "sort") " "))
               (("'rm -f ")
                (string-append "'" (which "rm") " -f "))
               (("'Rscript'") (string-append "'" (which "Rscript") "'")))
             (substitute* "TEToolkit/IO/ReadInputs.py"
               (("BamToBED") (which "bamToBed")))
             (substitute* "TEToolkit/Normalization.py"
               (("\"Rscript\"")
                (string-append "\"" (which "Rscript") "\"")))
             #t))
         (add-after 'install 'wrap-program
           (lambda* (#:key outputs #:allow-other-keys)
             ;; Make sure the executables find R packages.
             (let ((out (assoc-ref outputs "out")))
               (for-each
                (lambda (script)
                  (wrap-program (string-append out "/bin/" script)
                    `("R_LIBS_SITE" ":" = (,(getenv "R_LIBS_SITE")))))
                '("TEtranscripts"
                  "TEcount")))
             #t)))))
    (inputs
     `(("coreutils" ,coreutils)
       ("bedtools" ,bedtools)
       ("python-argparse" ,python2-argparse)
       ("python-pysam" ,python2-pysam)
       ("r-minimal" ,r-minimal)
       ("r-deseq2" ,r-deseq2)))
    (home-page "https://github.com/mhammell-laboratory/tetoolkit")
    (synopsis "Transposable elements in differential enrichment analysis")
    (description
     "This is package for including transposable elements in differential
enrichment analysis of sequencing datasets.  TEtranscripts and TEcount take
RNA-seq (and similar data) and annotates reads to both genes and transposable
elements.  TEtranscripts then performs differential analysis using DESeq2.
Note that TEtranscripts and TEcount rely on specially curated GTF files, which
are not included due to their size.")
    (license license:gpl3+)))

(define-public cd-hit
  (package
    (name "cd-hit")
    (version "4.6.8")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://github.com/weizhongli/cdhit"
                                  "/releases/download/V" version
                                  "/cd-hit-v" version
                                  "-2017-0621-source.tar.gz"))
              (sha256
               (base32
                "1b4mwm2520ixjbw57sil20f9iixzw4bkdqqwgg1fc3pzm6rz4zmn"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; there are no tests
       #:make-flags
       ;; Executables are copied directly to the PREFIX.
       (list (string-append "PREFIX=" (assoc-ref %outputs "out") "/bin")
             ;; Support longer sequences (e.g. Pacbio sequences)
             "MAX_SEQ=60000000")
       #:phases
       (modify-phases %standard-phases
         ;; No "configure" script
         (delete 'configure)
         ;; Remove sources of non-determinism
         (add-after 'unpack 'be-timeless
           (lambda _
             (substitute* "cdhit-utility.c++"
               ((" \\(built on \" __DATE__ \"\\)") ""))
             (substitute* "cdhit-common.c++"
               (("__DATE__") "\"0\"")
               (("\", %s, \" __TIME__ \"\\\\n\", date") ""))
             #t))
         ;; The "install" target does not create the target directory.
         (add-before 'install 'create-target-dir
           (lambda* (#:key outputs #:allow-other-keys)
             (mkdir-p (string-append (assoc-ref outputs "out") "/bin"))
             #t)))))
    (inputs
     `(("perl" ,perl)))
    (home-page "http://weizhongli-lab.org/cd-hit/")
    (synopsis "Cluster and compare protein or nucleotide sequences")
    (description
     "CD-HIT is a program for clustering and comparing protein or nucleotide
sequences.  CD-HIT is designed to be fast and handle extremely large
databases.")
    ;; The manual says: "It can be copied under the GNU General Public License
    ;; version 2 (GPLv2)."
    (license license:gpl2)))

(define-public clipper
  (package
    (name "clipper")
    (version "2.0")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/YeoLab/clipper")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1bcag4lb5bkzsj2vg7lrq24aw6yfgq275ifrbhd82l7kqgbbjbkv"))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-before 'reset-gzip-timestamps 'make-files-writable
           (lambda* (#:key outputs #:allow-other-keys)
             ;; Make sure .gz files are writable so that the
             ;; 'reset-gzip-timestamps' phase can do its work.
             (let ((out (assoc-ref outputs "out")))
               (for-each make-file-writable
                         (find-files out "\\.gz$"))
               #t))))))
    (inputs
     `(("htseq" ,htseq)
       ("python-pybedtools" ,python-pybedtools)
       ("python-cython" ,python-cython)
       ("python-scikit-learn" ,python-scikit-learn)
       ("python-matplotlib" ,python-matplotlib)
       ("python-pandas" ,python-pandas)
       ("python-pysam" ,python-pysam)
       ("python-numpy" ,python-numpy)
       ("python-scipy" ,python-scipy)))
    (native-inputs
     `(("python-setuptools-git" ,python-setuptools-git)
       ("python-mock" ,python-mock)   ; for tests
       ("python-nose" ,python-nose)   ; for tests
       ("python-pytz" ,python-pytz))) ; for tests
    (home-page "https://github.com/YeoLab/clipper")
    (synopsis "CLIP peak enrichment recognition")
    (description
     "CLIPper is a tool to define peaks in CLIP-seq datasets.")
    (license license:gpl2)))

(define-public codingquarry
  (package
    (name "codingquarry")
    (version "2.0")
    (source (origin
              (method url-fetch)
              (uri (string-append
                    "mirror://sourceforge/codingquarry/CodingQuarry_v"
                    version ".tar.gz"))
              (sha256
               (base32
                "0115hkjflsnfzn36xppwf9h9avfxlavr43djqmshkkzbgjzsz60i"))))
    (build-system gnu-build-system)
    (arguments
     '(#:tests? #f ; no "check" target
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin"))
                    (doc (string-append out "/share/doc/codingquarry")))
               (install-file "INSTRUCTIONS.pdf" doc)
               (copy-recursively "QuarryFiles"
                                 (string-append out "/QuarryFiles"))
               (install-file "CodingQuarry" bin)
               (install-file "CufflinksGTF_to_CodingQuarryGFF3.py" bin))
             #t)))))
    (inputs `(("openmpi" ,openmpi)))
    (native-search-paths
     (list (search-path-specification
            (variable "QUARRY_PATH")
            (files '("QuarryFiles")))))
    (native-inputs `(("python" ,python-2))) ; Only Python 2 is supported
    (synopsis "Fungal gene predictor")
    (description "CodingQuarry is a highly accurate, self-training GHMM fungal
gene predictor designed to work with assembled, aligned RNA-seq transcripts.")
    (home-page "https://sourceforge.net/projects/codingquarry/")
    (license license:gpl3+)))

(define-public couger
  (package
    (name "couger")
    (version "1.8.2")
    (source (origin
              (method url-fetch)
              (uri (string-append
                    "http://couger.oit.duke.edu/static/assets/COUGER"
                    version ".zip"))
              (sha256
               (base32
                "04p2b14nmhzxw5h72mpzdhalv21bx4w9b87z0wpw0xzxpysyncmq"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (delete 'build)
         (replace
          'install
          (lambda* (#:key outputs #:allow-other-keys)
            (let* ((out (assoc-ref outputs "out"))
                   (bin (string-append out "/bin")))
              (copy-recursively "src" (string-append out "/src"))
              (mkdir bin)
              ;; Add "src" directory to module lookup path.
              (substitute* "couger"
                (("from argparse")
                 (string-append "import sys\nsys.path.append(\""
                                out "\")\nfrom argparse")))
              (install-file "couger" bin))
            #t))
         (add-after
          'install 'wrap-program
          (lambda* (#:key inputs outputs #:allow-other-keys)
            ;; Make sure 'couger' runs with the correct PYTHONPATH.
            (let* ((out (assoc-ref outputs "out"))
                   (path (getenv "PYTHONPATH")))
              (wrap-program (string-append out "/bin/couger")
                `("PYTHONPATH" ":" prefix (,path))))
            #t)))))
    (inputs
     `(("python" ,python-2)
       ("python2-pillow" ,python2-pillow)
       ("python2-numpy" ,python2-numpy)
       ("python2-scipy" ,python2-scipy)
       ("python2-matplotlib" ,python2-matplotlib)))
    (propagated-inputs
     `(("r-minimal" ,r-minimal)
       ("libsvm" ,libsvm)
       ("randomjungle" ,randomjungle)))
    (native-inputs
     `(("unzip" ,unzip)))
    (home-page "http://couger.oit.duke.edu")
    (synopsis "Identify co-factors in sets of genomic regions")
    (description
     "COUGER can be applied to any two sets of genomic regions bound by
paralogous TFs (e.g., regions derived from ChIP-seq experiments) to identify
putative co-factors that provide specificity to each TF.  The framework
determines the genomic targets uniquely-bound by each TF, and identifies a
small set of co-factors that best explain the in vivo binding differences
between the two TFs.

COUGER uses classification algorithms (support vector machines and random
forests) with features that reflect the DNA binding specificities of putative
co-factors.  The features are generated either from high-throughput TF-DNA
binding data (from protein binding microarray experiments), or from large
collections of DNA motifs.")
    (license license:gpl3+)))

(define-public clustal-omega
  (package
    (name "clustal-omega")
    (version "1.2.4")
    (source (origin
              (method url-fetch)
              (uri (string-append "http://www.clustal.org/omega/clustal-omega-"
                                  version ".tar.gz"))
              (sha256
               (base32
                "1vm30mzncwdv881vrcwg11vzvrsmwy4wg80j5i0lcfk6dlld50w6"))))
    (build-system gnu-build-system)
    (inputs
     `(("argtable" ,argtable)))
    (home-page "http://www.clustal.org/omega/")
    (synopsis "Multiple sequence aligner for protein and DNA/RNA")
    (description
     "Clustal-Omega is a general purpose multiple sequence alignment (MSA)
program for protein and DNA/RNA.  It produces high quality MSAs and is capable
of handling data-sets of hundreds of thousands of sequences in reasonable
time.")
    (license license:gpl2+)))

(define-public crossmap
  (package
    (name "crossmap")
    (version "0.3.8")
    (source (origin
              (method url-fetch)
              (uri (pypi-uri "CrossMap" version))
              (sha256
               (base32
                "1sb2f2qbxya4fzw3yjl09vbrs8vfmw22zrygrvz004sf9gb1vkan"))))
    (build-system python-build-system)
    (inputs
     `(("python-bx-python" ,python-bx-python)
       ("python-numpy" ,python-numpy)
       ("python-pybigwig" ,python-pybigwig)
       ("python-pysam" ,python-pysam)
       ("zlib" ,zlib)))
    (native-inputs
     `(("python-cython" ,python-cython)
       ("python-nose" ,python-nose)))
    (home-page "http://crossmap.sourceforge.net/")
    (synopsis "Convert genome coordinates between assemblies")
    (description
     "CrossMap is a program for conversion of genome coordinates or annotation
files between different genome assemblies.  It supports most commonly used
file formats including SAM/BAM, Wiggle/BigWig, BED, GFF/GTF, VCF.")
    (license license:gpl2+)))

(define-public python-dnaio
  (package
    (name "python-dnaio")
    (version "0.3")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "dnaio" version))
       (sha256
        (base32
         "0f16m7hdlm0fz1n7y5asy0v9ghyrq17ni1p9iybq22ddzyd49r27"))))
    (build-system python-build-system)
    (native-inputs
     `(("python-cython" ,python-cython)
       ("python-pytest" ,python-pytest)
       ("python-xopen" ,python-xopen)))
    (home-page "https://github.com/marcelm/dnaio/")
    (synopsis "Read FASTA and FASTQ files efficiently")
    (description
     "dnaio is a Python library for fast parsing of FASTQ and also FASTA
files.  The code was previously part of the cutadapt tool.")
    (license license:expat)))

(define-public python-deeptoolsintervals
  (package
    (name "python-deeptoolsintervals")
    (version "0.1.9")
    (source (origin
              (method url-fetch)
              (uri (pypi-uri "deeptoolsintervals" version))
              (sha256
               (base32
                "1xnl80nblysj6dylj4683wgrfa425rkx4dp5k65hvwdns9pw753x"))))
    (build-system python-build-system)
    (inputs
     `(("zlib" ,zlib)))
    (home-page "https://github.com/deeptools/deeptools_intervals")
    (synopsis "Create GTF-based interval trees with associated meta-data")
    (description
     "This package provides a Python module creating/accessing GTF-based
interval trees with associated meta-data.  It is primarily used by the
@code{deeptools} package.")
    (license license:expat)))

(define-public python-deeptools
  (package
    (name "python-deeptools")
    (version "3.4.3")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/deeptools/deepTools")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "0l09vyynz6s6w7fnyd94rpys4a6aja6kp4gli64pngdxdz3md1nl"))))
    (build-system python-build-system)
    (native-inputs
     `(("python-mock" ,python-mock)
       ("python-nose" ,python-nose)))
    (propagated-inputs
     `(("python-matplotlib" ,python-matplotlib)
       ("python-numpy" ,python-numpy)
       ("python-numpydoc" ,python-numpydoc)
       ("python-py2bit" ,python-py2bit)
       ("python-pybigwig" ,python-pybigwig)
       ("python-pysam" ,python-pysam)
       ("python-scipy" ,python-scipy)
       ("python-deeptoolsintervals" ,python-deeptoolsintervals)
       ("python-plotly" ,python-plotly-2.4.1)))
    (home-page "https://pypi.org/project/deepTools/")
    (synopsis "Useful tools for exploring deep sequencing data")
    (description "This package addresses the challenge of handling large amounts
of data that are now routinely generated from DNA sequencing centers.
@code{deepTools} contains useful modules to process the mapped reads data for
multiple quality checks, creating normalized coverage files in standard bedGraph
and bigWig file formats, that allow comparison between different files.  Finally,
using such normalized and standardized files, deepTools can create many
publication-ready visualizations to identify enrichments and for functional
annotations of the genome.")
    ;; The file deeptools/cm.py is licensed under the BSD license.  The
    ;; remainder of the code is licensed under the MIT license.
    (license (list license:bsd-3 license:expat))))

(define-deprecated deeptools python-deeptools)

(define-public cutadapt
  (package
    (name "cutadapt")
    (version "2.1")
    (source (origin
              (method url-fetch)
              (uri (pypi-uri "cutadapt" version))
              (sha256
               (base32
                "1vqmsfkm6llxzmsz9wcfcvzx9a9f8iabvwik2rbyn7nc4wm25z89"))))
    (build-system python-build-system)
    (inputs
     `(("python-dnaio" ,python-dnaio)
       ("python-xopen" ,python-xopen)))
    (native-inputs
     `(("python-cython" ,python-cython)
       ("python-pytest" ,python-pytest)
       ("python-setuptools-scm" ,python-setuptools-scm)))
    (home-page "https://cutadapt.readthedocs.io/en/stable/")
    (synopsis "Remove adapter sequences from nucleotide sequencing reads")
    (description
     "Cutadapt finds and removes adapter sequences, primers, poly-A tails and
other types of unwanted sequence from high-throughput sequencing reads.")
    (license license:expat)))

(define-public libbigwig
  (package
    (name "libbigwig")
    (version "0.4.4")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/dpryan79/libBigWig")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "09693dmf1scdac5pyq6qyn8b4mcipvnmc370k9a5z41z81m3dcsj"))))
    (build-system gnu-build-system)
    (arguments
     `(#:test-target "test"
       #:tests? #f ; tests require access to the web
       #:make-flags
       (list "CC=gcc"
             (string-append "prefix=" (assoc-ref %outputs "out")))
       #:phases
       (modify-phases %standard-phases
         (delete 'configure))))
    (inputs
     `(("zlib" ,zlib)
       ("curl" ,curl)))
    (native-inputs
     `(("doxygen" ,doxygen)
       ;; Need for tests
       ("python" ,python-2)))
    (home-page "https://github.com/dpryan79/libBigWig")
    (synopsis "C library for handling bigWig files")
    (description
     "This package provides a C library for parsing local and remote BigWig
files.")
    (license license:expat)))

(define-public python-pybigwig
  (package
    (name "python-pybigwig")
    (version "0.3.17")
    (source (origin
              (method url-fetch)
              (uri (pypi-uri "pyBigWig" version))
              (sha256
               (base32
                "157x6v48y299zm382krf1dw08fdxg95im8lnabhp5vc94s04zxj1"))
              (modules '((guix build utils)))
              (snippet
               '(begin
                  ;; Delete bundled libBigWig sources
                  (delete-file-recursively "libBigWig")
                  #t))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'link-with-libBigWig
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* "setup.py"
               (("libs=\\[") "libs=[\"BigWig\", "))
             #t)))))
    (propagated-inputs
     `(("python-numpy" ,python-numpy)))
    (inputs
     `(("libbigwig" ,libbigwig)
       ("zlib" ,zlib)
       ("curl" ,curl)))
    (home-page "https://github.com/dpryan79/pyBigWig")
    (synopsis "Access bigWig files in Python using libBigWig")
    (description
     "This package provides Python bindings to the libBigWig library for
accessing bigWig files.")
    (license license:expat)))

(define-public python2-pybigwig
  (package-with-python2 python-pybigwig))

(define-public python-schema-salad
  (package
    (name "python-schema-salad")
    (version "7.0.20200811075006")
    (source
      (origin
        (method url-fetch)
        (uri (pypi-uri "schema-salad" version))
        (sha256
         (base32
          "0wanbwmqb189x1m0vacnhpivfsr8rwbqknngivzxxs8j46yj80bg"))))
    (build-system python-build-system)
    (propagated-inputs
     `(("python-cachecontrol" ,python-cachecontrol-0.11)
       ("python-lockfile" ,python-lockfile)
       ("python-mistune" ,python-mistune)
       ("python-rdflib" ,python-rdflib)
       ("python-rdflib-jsonld" ,python-rdflib-jsonld)
       ("python-requests" ,python-requests)
       ("python-ruamel.yaml" ,python-ruamel.yaml)
       ("python-typing-extensions" ,python-typing-extensions)))
    (native-inputs
     `(("python-pytest" ,python-pytest)
       ("python-pytest-runner" ,python-pytest-runner)))
    (home-page "https://github.com/common-workflow-language/schema_salad")
    (synopsis "Schema Annotations for Linked Avro Data (SALAD)")
    (description
     "Salad is a schema language for describing JSON or YAML structured linked
data documents.  Salad schema describes rules for preprocessing, structural
validation, and hyperlink checking for documents described by a Salad schema.
Salad supports rich data modeling with inheritance, template specialization,
object identifiers, object references, documentation generation, code
generation, and transformation to RDF.  Salad provides a bridge between document
and record oriented data modeling and the Semantic Web.")
    (license license:asl2.0)))

(define-public cwltool
  (package
    (name "cwltool")
    (version "3.0.20201121085451")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/common-workflow-language/cwltool")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1awf99n7aglxc5zszrlrv6jxp355jp45ws7wpsgjlgcdv7advn0w"))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'loosen-version-restrictions
           (lambda _
             (substitute* "setup.py"
               (("== 1.5.1") ">=1.5.1") ; prov
               ((", < 3.5") ""))        ; shellescape
             #t))
         (add-after 'unpack 'dont-use-git
           (lambda _
             (substitute* "gittaggers.py"
               (("self.git_timestamp_tag\\(\\)")
                (string-append "time.strftime('.%Y%m%d%H%M%S', time.gmtime(int("
                               (string-drop ,version 4) ")))")))
             #t))
         (add-after 'unpack 'modify-tests
           (lambda _
             ;; Tries to connect to the internet.
             (delete-file "tests/test_udocker.py")
             (delete-file "tests/test_http_input.py")
             (substitute* "tests/test_load_tool.py"
               (("def test_load_graph_fragment_from_packed")
                (string-append "@pytest.mark.skip(reason=\"Disabled by Guix\")\n"
                               "def test_load_graph_fragment_from_packed")))
             (substitute* "tests/test_examples.py"
               (("def test_env_filtering")
                (string-append "@pytest.mark.skip(reason=\"Disabled by Guix\")\n"
                               "def test_env_filtering")))
             ;; Tries to use cwl-runners.
             (substitute* "tests/test_examples.py"
               (("def test_v1_0_arg_empty_prefix_separate_false")
                (string-append "@pytest.mark.skip(reason=\"Disabled by Guix\")\n"
                               "def test_v1_0_arg_empty_prefix_separate_false")))
             #t)))))
    (propagated-inputs
     `(("python-argcomplete" ,python-argcomplete)
       ("python-bagit" ,python-bagit)
       ("python-coloredlogs" ,python-coloredlogs)
       ("python-mypy-extensions" ,python-mypy-extensions)
       ("python-prov" ,python-prov)
       ("python-pydot" ,python-pydot)
       ("python-psutil" ,python-psutil)
       ("python-rdflib" ,python-rdflib)
       ("python-requests" ,python-requests)
       ("python-ruamel.yaml" ,python-ruamel.yaml)
       ("python-schema-salad" ,python-schema-salad)
       ("python-shellescape" ,python-shellescape)
       ("python-typing-extensions" ,python-typing-extensions)
       ;; Not listed as needed but still necessary:
       ("node" ,node)))
    (native-inputs
     `(("python-arcp" ,python-arcp)
       ("python-humanfriendly" ,python-humanfriendly)
       ("python-mock" ,python-mock)
       ("python-pytest" ,python-pytest)
       ("python-pytest-cov" ,python-pytest-cov)
       ("python-pytest-mock" ,python-pytest-mock)
       ("python-pytest-runner" ,python-pytest-runner)
       ("python-rdflib-jsonld" ,python-rdflib-jsonld)))
    (home-page
     "https://github.com/common-workflow-language/common-workflow-language")
    (synopsis "Common Workflow Language reference implementation")
    (description
     "This is the reference implementation of the @acronym{CWL, Common Workflow
Language} standards.  The CWL open standards are for describing analysis
workflows and tools in a way that makes them portable and scalable across a
variety of software and hardware environments, from workstations to cluster,
cloud, and high performance computing (HPC) environments.  CWL is designed to
meet the needs of data-intensive science, such as Bioinformatics, Medical
Imaging, Astronomy, Physics, and Chemistry.  The @acronym{cwltool, CWL reference
implementation} is intended to be feature complete and to provide comprehensive
validation of CWL files as well as provide other tools related to working with
CWL descriptions.")
    (license license:asl2.0)))

(define-public python-dendropy
  (package
    (name "python-dendropy")
    (version "4.4.0")
    (source
     (origin
       (method git-fetch)
       ;; Source from GitHub so that tests are included.
       (uri (git-reference
             (url "https://github.com/jeetsukumaran/DendroPy")
             (commit (string-append "v" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "097hfyv2kaf4x92i4rjx0paw2cncxap48qivv8zxng4z7nhid0x9"))))
    (build-system python-build-system)
    (home-page "https://dendropy.org/")
    (synopsis "Library for phylogenetics and phylogenetic computing")
    (description
     "DendroPy is a library for phylogenetics and phylogenetic computing: reading,
writing, simulation, processing and manipulation of phylogenetic
trees (phylogenies) and characters.")
    (license license:bsd-3)))

(define-public python2-dendropy
  (let ((base (package-with-python2 python-dendropy)))
    (package
      (inherit base)
      (arguments
       `(#:phases
         (modify-phases %standard-phases
           (add-after 'unpack 'remove-failing-test
             (lambda _
               ;; This test fails when the full test suite is run, as documented
               ;; at https://github.com/jeetsukumaran/DendroPy/issues/74
               (substitute* "tests/test_dataio_nexml_reader_tree_list.py"
                 (("test_collection_comments_and_annotations")
                  "do_not_test_collection_comments_and_annotations"))
               #t)))
         ,@(package-arguments base))))))

(define-public python-py2bit
  (package
    (name "python-py2bit")
    (version "0.3.0")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "py2bit" version))
       (sha256
        (base32
         "1vw2nvw1yrl7ikkqsqs1pg239yr5nspvd969r1x9arms1k25a1a5"))))
    (build-system python-build-system)
    (home-page "https://github.com/dpryan79/py2bit")
    (synopsis "Access 2bit files using lib2bit")
    (description
     "This package provides Python bindings for lib2bit to access 2bit files
with Python.")
    (license license:expat)))

(define-public delly
  (package
    (name "delly")
    (version "0.8.3")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/dellytools/delly")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32 "1ibnplgfzj96w8glkx17v7sld3pm402fr5ybmf3h0rlcryabxrqy"))
              (modules '((guix build utils)))
              (snippet
               '(begin
                  (delete-file-recursively "src/htslib")
                  #t))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; There are no tests to run.
       #:make-flags
       (list "PARALLEL=1"  ; Allow parallel execution at run-time.
             (string-append "prefix=" (assoc-ref %outputs "out")))
       #:phases
       (modify-phases %standard-phases
         (delete 'configure) ; There is no configure phase.
         (add-after 'install 'install-templates
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((templates (string-append (assoc-ref outputs "out")
                                             "/share/delly/templates")))
               (mkdir-p templates)
               (copy-recursively "excludeTemplates" templates)
               #t))))))
    (inputs
     `(("boost" ,boost)
       ("bzip2" ,bzip2)
       ("htslib" ,htslib)
       ("zlib" ,zlib)))
    (home-page "https://github.com/dellytools/delly")
    (synopsis "Integrated structural variant prediction method")
    (description "Delly is an integrated structural variant prediction method
that can discover and genotype deletions, tandem duplications, inversions and
translocations at single-nucleotide resolution in short-read massively parallel
sequencing data.  It uses paired-ends and split-reads to sensitively and
accurately delineate genomic rearrangements throughout the genome.")
    (license license:gpl3+)))

(define-public diamond
  (package
    (name "diamond")
    (version "0.9.30")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/bbuchfink/diamond")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "0k6f3kb6cniw11xw6763kkbs1sl0yack7xsy7q5fl5v170ssphq4"))))
    (build-system cmake-build-system)
    (arguments
     '(#:tests? #f ; no "check" target
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'remove-native-compilation
           (lambda _
             (substitute* "CMakeLists.txt" (("-march=native") ""))
             #t)))))
    (inputs
     `(("zlib" ,zlib)))
    (home-page "https://github.com/bbuchfink/diamond")
    (synopsis "Accelerated BLAST compatible local sequence aligner")
    (description
     "DIAMOND is a BLAST-compatible local aligner for mapping protein and
translated DNA query sequences against a protein reference database (BLASTP
and BLASTX alignment mode).  The speedup over BLAST is up to 20,000 on short
reads at a typical sensitivity of 90-99% relative to BLAST depending on the
data and settings.")
    (license license:agpl3+)))

(define-public discrover
  (package
    (name "discrover")
    (version "1.6.0")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/maaskola/discrover")
             (commit version)))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "173fwi2vb6a5kp406hm3jj6j7v4whww796f2qcygp4rpvamh307y"))))
    (build-system cmake-build-system)
    (arguments
     `(#:tests? #f                      ; there are no tests
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'fix-latex-errors
           (lambda _
             (with-fluids ((%default-port-encoding #f))
               (substitute* "doc/references.bib"
                 (("\\{S\\}illanp[^,]+,")
                  "{S}illanp{\\\"a}{\\\"a},")))
             ;; XXX: I just can't get pdflatex to not complain about these
             ;; characters.  They end up in the manual via the generated
             ;; discrover-cli-help.txt.
             (substitute* "src/hmm/cli.cpp"
               (("µ") "mu")
               (("η") "eta")
               (("≤") "<="))
             ;; This seems to be a syntax error.
             (substitute* "doc/discrover-manual.tex"
               (("theverbbox\\[t\\]") "theverbbox"))
             #t))
         (add-after 'unpack 'add-missing-includes
           (lambda _
             (substitute* "src/executioninformation.hpp"
               (("#define EXECUTIONINFORMATION_HPP" line)
                (string-append line "\n#include <random>")))
             (substitute* "src/plasma/fasta.hpp"
               (("#define FASTA_HPP" line)
                (string-append line "\n#include <random>")))
             #t))
         ;; FIXME: this is needed because we're using texlive-union, which
         ;; doesn't handle fonts correctly.  It expects to be able to generate
         ;; fonts in the home directory.
         (add-before 'build 'setenv-HOME
           (lambda _ (setenv "HOME" "/tmp") #t)))))
    (inputs
     `(("boost" ,boost)
       ("cairo" ,cairo)
       ("rmath-standalone" ,rmath-standalone)))
    (native-inputs
     `(("texlive" ,(texlive-union (list texlive-fonts-cm
                                        texlive-fonts-amsfonts

                                        texlive-latex-doi
                                        texlive-latex-examplep
                                        texlive-latex-hyperref
                                        texlive-latex-ms
                                        texlive-latex-natbib
                                        texlive-bibtex         ; style files used by natbib
                                        texlive-latex-pgf      ; tikz
                                        texlive-latex-verbatimbox)))
       ("imagemagick" ,imagemagick)))
    (home-page "https://dorina.mdc-berlin.de/public/rajewsky/discrover/")
    (synopsis "Discover discriminative nucleotide sequence motifs")
    (description "Discrover is a motif discovery method to find binding sites
of nucleic acid binding proteins.")
    (license license:gpl3+)))

(define-public eigensoft
  (package
    (name "eigensoft")
    (version "7.2.1")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/DReichLab/EIG")
             (commit (string-append "v" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "1c141fqvhnzibmnf22sv23vbmzm20kjjyrib44cfh75wyndp2d9k"))
       (modules '((guix build utils)))
       ;; Remove pre-built binaries.
       (snippet '(begin
                   (delete-file-recursively "bin")
                   (mkdir "bin")
                   #t))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f                    ; There are no tests.
       #:make-flags '("CC=gcc")
       #:phases
       (modify-phases %standard-phases
         ;; There is no configure phase, but the Makefile is in a
         ;; sub-directory.
         (replace 'configure
           (lambda _ (chdir "src") #t))
         ;; The provided install target only copies executables to
         ;; the "bin" directory in the build root.
         (add-after 'install 'actually-install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin  (string-append out "/bin")))
               (for-each (lambda (file)
                           (install-file file bin))
                         (find-files "../bin" ".*"))
               #t))))))
    (inputs
     `(("gsl" ,gsl)
       ("lapack" ,lapack)
       ("openblas" ,openblas)
       ("perl" ,perl)
       ("gfortran" ,gfortran "lib")))
    (home-page "https://github.com/DReichLab/EIG")
    (synopsis "Tools for population genetics")
    (description "The EIGENSOFT package provides tools for population
genetics and stratification correction.  EIGENSOFT implements methods commonly
used in population genetics analyses such as PCA, computation of Tracy-Widom
statistics, and finding related individuals in structured populations.  It
comes with a built-in plotting script and supports multiple file formats and
quantitative phenotypes.")
    ;; The license of the eigensoft tools is Expat, but since it's
    ;; linking with the GNU Scientific Library (GSL) the effective
    ;; license is the GPL.
    (license license:gpl3+)))

(define-public edirect
  (package
    (name "edirect")
    (version "13.3.20200128")
    (source (origin
              (method url-fetch)
              (uri (string-append "ftp://ftp.ncbi.nlm.nih.gov/entrez/entrezdirect"
                                  "/versions/" version
                                  "/edirect-" version ".tar.gz"))
              (sha256
               (base32
                "093zp7klv81ph0y8mm8d78a9hnpfxbv2kdym70gzdf3vz176rw33"))
              (modules '((guix build utils)))
              (snippet
               '(begin (delete-file "Mozilla-CA.tar.gz")
                       (substitute* "rchive.go"
                         ;; This go library does not have any license.
                         (("github.com/fiam/gounidecode/unidecode")
                          "golang.org/rainycape/unidecode"))
                       #t))))
    (build-system perl-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (delete 'build)
         (delete 'check)                ; simple check after install
         (add-after 'unpack 'patch-programs
           (lambda* (#:key inputs #:allow-other-keys)
             ;; Ignore errors about missing xtract.Linux and rchive.Linux.
              (substitute* "pm-refresh"
                (("cat \\\"\\$target")
                 "grep ^[[:digit:]] \"$target"))
              #t))
         (replace 'install
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let ((bin (string-append (assoc-ref outputs "out") "/bin"))
                   (edirect-go (assoc-ref inputs "edirect-go-programs")))
               (for-each
                 (lambda (file)
                   (install-file file bin))
                 '("archive-pubmed" "asp-cp" "asp-ls" "download-ncbi-data"
                   "download-pubmed" "edirect.pl" "efetch" "epost" "esearch"
                   "fetch-pubmed" "ftp-cp" "ftp-ls" "has-asp" "index-pubmed"
                   "pm-prepare" "pm-refresh" "pm-stash" "pm-collect"
                   "pm-index" "pm-invert" "pm-merge" "pm-promote"))
               (symlink (string-append edirect-go "/bin/xtract.Linux")
                        (string-append bin "/xtract"))
               (symlink (string-append edirect-go "/bin/rchive.Linux")
                        (string-append bin "/rchive")))
             #t))
         (add-after 'install 'wrap-program
           (lambda* (#:key outputs #:allow-other-keys)
              ;; Make sure everything can run in a pure environment.
              (let ((out (assoc-ref outputs "out"))
                    (path (getenv "PERL5LIB")))
                (for-each
                  (lambda (file)
                    (wrap-program file
                      `("PERL5LIB" ":" prefix (,path)))
                    (wrap-program file
                      `("PATH" ":" prefix (,(string-append out "/bin")
                                           ,(dirname (which "sed"))
                                           ,(dirname (which "gzip"))
                                           ,(dirname (which "grep"))
                                           ,(dirname (which "perl"))
                                           ,(dirname (which "uname"))))))
                  (find-files out ".")))
              #t))
         (add-after 'wrap-program 'check
           (lambda* (#:key outputs #:allow-other-keys)
             (invoke (string-append (assoc-ref outputs "out")
                                    "/bin/edirect.pl")
                     "-filter" "-help")
             #t)))))
    (inputs
     `(("edirect-go-programs" ,edirect-go-programs)
       ("perl-html-parser" ,perl-html-parser)
       ("perl-encode-locale" ,perl-encode-locale)
       ("perl-file-listing" ,perl-file-listing)
       ("perl-html-tagset" ,perl-html-tagset)
       ("perl-html-tree" ,perl-html-tree)
       ("perl-http-cookies" ,perl-http-cookies)
       ("perl-http-date" ,perl-http-date)
       ("perl-http-message" ,perl-http-message)
       ("perl-http-negotiate" ,perl-http-negotiate)
       ("perl-lwp-mediatypes" ,perl-lwp-mediatypes)
       ("perl-lwp-protocol-https" ,perl-lwp-protocol-https)
       ("perl-net-http" ,perl-net-http)
       ("perl-uri" ,perl-uri)
       ("perl-www-robotrules" ,perl-www-robotrules)
       ("perl-xml-simple" ,perl-xml-simple)
       ("perl" ,perl)))
    (home-page "https://www.ncbi.nlm.nih.gov/books/NBK179288/")
    (synopsis "Tools for accessing the NCBI's set of databases")
    (description
     "Entrez Direct (EDirect) is a method for accessing the National Center
for Biotechnology Information's (NCBI) set of interconnected
databases (publication, sequence, structure, gene, variation, expression,
etc.) from a terminal.  Functions take search terms from command-line
arguments.  Individual operations are combined to build multi-step queries.
Record retrieval and formatting normally complete the process.

EDirect also provides an argument-driven function that simplifies the
extraction of data from document summaries or other results that are returned
in structured XML format.  This can eliminate the need for writing custom
software to answer ad hoc questions.")
    (native-search-paths
     ;; Ideally this should be set for LWP somewhere.
     (list (search-path-specification
            (variable "PERL_LWP_SSL_CA_FILE")
            (file-type 'regular)
            (separator #f)
            (files '("/etc/ssl/certs/ca-certificates.crt")))))
    (license license:public-domain)))

(define-public edirect-go-programs
  (package
    (inherit edirect)
    (name "edirect-go-programs")
    (build-system go-build-system)
    (arguments
     `(#:install-source? #f
       #:tests? #f      ; No tests.
       #:import-path "ncbi.nlm.nih.gov/entrez/edirect"
       #:phases
       (modify-phases %standard-phases
         (replace 'build
           (lambda* (#:key import-path #:allow-other-keys)
             (with-directory-excursion (string-append "src/" import-path)
               (invoke "go" "build" "-v" "-x" "j2x.go")
               (invoke "go" "build" "-v" "-x" "t2x.go")
               (invoke "go" "build" "-v" "-x" "-o"
                       "xtract.Linux" "xtract.go" "common.go")
               (invoke "go" "build" "-v" "-x" "-o"
                       "rchive.Linux" "rchive.go" "common.go")
               (invoke "go" "build" "-v" "-x" "-o" "symbols.Linux" "s2p.go"))))
         (replace 'install
           (lambda* (#:key outputs import-path #:allow-other-keys)
             (let ((dest    (string-append (assoc-ref outputs "out") "/bin"))
                   (source  (string-append "src/" import-path "/")))
               (for-each (lambda (file)
                           (format #t "installing ~a~%" file)
                           (install-file (string-append source file) dest))
                         '("j2x" "t2x" "symbols.Linux" "xtract.Linux" "rchive.Linux"))
               #t))))))
    (native-inputs '())
    (propagated-inputs '())
    (inputs
     `(("go-github-com-fatih-color" ,go-github-com-fatih-color)
       ("go-github-com-fogleman-gg" ,go-github-com-fogleman-gg)
       ("go-github-com-gedex-inflector" ,go-github-com-gedex-inflector)
       ("go-github-com-golang-freetype" ,go-github-com-golang-freetype)
       ("go-github-com-klauspost-cpuid" ,go-github-com-klauspost-cpuid)
       ("go-github-com-pbnjay-memory" ,go-github-com-pbnjay-memory)
       ("go-github-com-surgebase-porter2" ,go-github-com-surgebase-porter2)
       ("go-golang-org-rainycape-unidecode" ,go-golang-org-rainycape-unidecode)
       ("go-golang-org-x-image" ,go-golang-org-x-image)
       ("go-golang-org-x-text" ,go-golang-org-x-text)))))

(define-public exonerate
  (package
    (name "exonerate")
    (version "2.4.0")
    (source
     (origin
       (method url-fetch)
       (uri
        (string-append
         "http://ftp.ebi.ac.uk/pub/software/vertebrategenomics/exonerate/"
         "exonerate-" version ".tar.gz"))
       (sha256
        (base32
         "0hj0m9xygiqsdxvbg79wq579kbrx1mdrabi2bzqz2zn9qwfjcjgq"))))
    (build-system gnu-build-system)
    (arguments
     `(#:parallel-build? #f)) ; Building in parallel fails on some machines.
    (native-inputs
     `(("pkg-config" ,pkg-config)))
    (inputs
     `(("glib" ,glib)))
    (home-page
     "https://www.ebi.ac.uk/about/vertebrate-genomics/software/exonerate")
    (synopsis "Generic tool for biological sequence alignment")
    (description
     "Exonerate is a generic tool for pairwise sequence comparison.  It allows
the alignment of sequences using a many alignment models, either exhaustive
dynamic programming or a variety of heuristics.")
    (license license:gpl3)))

(define-public express
  (package
    (name "express")
    (version "1.5.3")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/adarob/eXpress")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "18nb22n7x820fzjngf4qgyb3mspqkw7xyk7v7s5ps6wfrd8qwscb"))))
    (build-system cmake-build-system)
    (arguments
     `(#:tests? #f ;no "check" target
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'use-shared-boost-libs-and-set-bamtools-paths
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* "CMakeLists.txt"
               (("set\\(Boost_USE_STATIC_LIBS ON\\)")
                "set(Boost_USE_STATIC_LIBS OFF)")
               (("\\$\\{CMAKE_CURRENT_SOURCE_DIR\\}/bamtools/include")
                (string-append (assoc-ref inputs "bamtools") "/include/bamtools")))
             (substitute* "src/CMakeLists.txt"
               (("\\$\\{CMAKE_CURRENT_SOURCE_DIR\\}/\\.\\./bamtools/lib")
                (string-append (assoc-ref inputs "bamtools") "/lib"))
               (("libprotobuf.a") "libprotobuf.so"))
             #t))
         (add-after 'unpack 'remove-update-check
           (lambda _
             (substitute* "src/main.cpp"
               (("#include \"update_check.h\"") "")
               (("check_version\\(PACKAGE_VERSION\\);") ""))
             #t)))))
    (inputs
     `(("boost" ,boost)
       ("bamtools" ,bamtools)
       ("protobuf" ,protobuf)
       ("zlib" ,zlib)))
    (home-page "http://bio.math.berkeley.edu/eXpress")
    (synopsis "Streaming quantification for high-throughput genomic sequencing")
    (description
     "eXpress is a streaming tool for quantifying the abundances of a set of
target sequences from sampled subsequences.  Example applications include
transcript-level RNA-Seq quantification, allele-specific/haplotype expression
analysis (from RNA-Seq), transcription factor binding quantification in
ChIP-Seq, and analysis of metagenomic data.")
    (license license:artistic2.0)))

(define-public express-beta-diversity
  (package
   (name "express-beta-diversity")
   (version "1.0.8")
   (source (origin
             (method git-fetch)
             (uri (git-reference
                   (url "https://github.com/dparks1134/ExpressBetaDiversity")
                   (commit (string-append "v" version))))
             (file-name (git-file-name name version))
             (sha256
              (base32
               "0s0yzg5c21349rh7x4w9266jsvnp7j1hp9cf8sk32hz8nvrj745x"))))
   (build-system gnu-build-system)
   (arguments
    `(#:phases
      (modify-phases %standard-phases
        (delete 'configure)
        (add-before 'build 'enter-source (lambda _ (chdir "source") #t))
        (replace 'check
          (lambda _ (invoke "../bin/ExpressBetaDiversity" "-u") #t))
        (replace 'install
          (lambda* (#:key outputs #:allow-other-keys)
            (let ((bin (string-append (assoc-ref outputs "out") "/bin")))
              (install-file "../scripts/convertToEBD.py" bin)
              (install-file "../bin/ExpressBetaDiversity" bin)
              #t))))))
   (inputs
    `(("python" ,python-2)))
   (home-page "https://github.com/dparks1134/ExpressBetaDiversity")
   (synopsis "Taxon- and phylogenetic-based beta diversity measures")
   (description
    "Express Beta Diversity (EBD) calculates ecological beta diversity
(dissimilarity) measures between biological communities.  EBD implements a
variety of diversity measures including those that make use of phylogenetic
similarity of community members.")
   (license license:gpl3+)))

(define-public fasttree
  (package
   (name "fasttree")
   (version "2.1.10")
   (source (origin
             (method url-fetch)
             (uri (string-append
                   "http://www.microbesonline.org/fasttree/FastTree-"
                   version ".c"))
             (sha256
              (base32
               "0vcjdvy1j4m702vmak4svbfkrpcw63k7wymfksjp9a982zy8kjsl"))))
   (build-system gnu-build-system)
   (arguments
    `(#:tests? #f ; no "check" target
      #:phases
      (modify-phases %standard-phases
        (delete 'unpack)
        (delete 'configure)
        (replace 'build
          (lambda* (#:key source #:allow-other-keys)
            (invoke "gcc"
                    "-O3"
                    "-finline-functions"
                    "-funroll-loops"
                    "-Wall"
                    "-o"
                    "FastTree"
                    source
                    "-lm")
            (invoke "gcc"
                    "-DOPENMP"
                    "-fopenmp"
                    "-O3"
                    "-finline-functions"
                    "-funroll-loops"
                    "-Wall"
                    "-o"
                    "FastTreeMP"
                    source
                    "-lm")
            #t))
        (replace 'install
          (lambda* (#:key outputs #:allow-other-keys)
            (let ((bin (string-append (assoc-ref outputs "out") "/bin")))
              (install-file "FastTree" bin)
              (install-file "FastTreeMP" bin)
              #t))))))
   (home-page "http://www.microbesonline.org/fasttree")
   (synopsis "Infers approximately-maximum-likelihood phylogenetic trees")
   (description
    "FastTree can handle alignments with up to a million of sequences in a
reasonable amount of time and memory.  For large alignments, FastTree is
100-1,000 times faster than PhyML 3.0 or RAxML 7.")
   (license license:gpl2+)))

(define-public fastx-toolkit
  (package
    (name "fastx-toolkit")
    (version "0.0.14")
    (source (origin
              (method url-fetch)
              (uri
               (string-append
                "https://github.com/agordon/fastx_toolkit/releases/download/"
                version "/fastx_toolkit-" version ".tar.bz2"))
              (sha256
               (base32
                "01jqzw386873sr0pjp1wr4rn8fsga2vxs1qfmicvx1pjr72007wy"))))
    (build-system gnu-build-system)
    (inputs
     `(("libgtextutils" ,libgtextutils)))
    (native-inputs
     `(("gcc" ,gcc-6) ;; doesn't build with later versions
       ("pkg-config" ,pkg-config)))
    (home-page "http://hannonlab.cshl.edu/fastx_toolkit/")
    (synopsis "Tools for FASTA/FASTQ file preprocessing")
    (description
     "The FASTX-Toolkit is a collection of command line tools for Short-Reads
FASTA/FASTQ files preprocessing.

Next-Generation sequencing machines usually produce FASTA or FASTQ files,
containing multiple short-reads sequences.  The main processing of such
FASTA/FASTQ files is mapping the sequences to reference genomes.  However, it
is sometimes more productive to preprocess the files before mapping the
sequences to the genome---manipulating the sequences to produce better mapping
results.  The FASTX-Toolkit tools perform some of these preprocessing tasks.")
    (license license:agpl3+)))

(define-public flexbar
  (package
    (name "flexbar")
    (version "3.4.0")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/seqan/flexbar")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1pq9sxvdnldl14libk234m72dqhwgzs3acgl943wchwdqlcsi5r2"))))
    (build-system cmake-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'do-not-tune-to-CPU
           (lambda _
             (substitute* "src/CMakeLists.txt"
               ((" -march=native") ""))
             #t))
         (replace 'check
           (lambda* (#:key outputs #:allow-other-keys)
             (setenv "PATH" (string-append (getcwd) ":" (getenv "PATH")))
             (with-directory-excursion "../source/test"
               (invoke "bash" "flexbar_test.sh"))
             #t))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (string-append (assoc-ref outputs "out")))
                    (bin (string-append out "/bin/")))
               (install-file "flexbar" bin))
             #t)))))
    (inputs
     `(("tbb" ,tbb)
       ("zlib" ,zlib)))
    (native-inputs
     `(("pkg-config" ,pkg-config)
       ("seqan" ,seqan)))
    (home-page "https://github.com/seqan/flexbar")
    (synopsis "Barcode and adapter removal tool for sequencing platforms")
    (description
     "Flexbar preprocesses high-throughput nucleotide sequencing data
efficiently.  It demultiplexes barcoded runs and removes adapter sequences.
Moreover, trimming and filtering features are provided.  Flexbar increases
read mapping rates and improves genome and transcriptome assemblies.  It
supports next-generation sequencing data in fasta/q and csfasta/q format from
Illumina, Roche 454, and the SOLiD platform.")
    (license license:bsd-3)))

(define-public fraggenescan
  (package
    (name "fraggenescan")
    (version "1.30")
    (source
     (origin
       (method url-fetch)
       (uri
        (string-append "mirror://sourceforge/fraggenescan/"
                       "FragGeneScan" version ".tar.gz"))
       (sha256
        (base32 "158dcnwczgcyhwm4qlx19sanrwgdpzf6bn2y57mbpx55lkgz1mzj"))))
    (build-system gnu-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (add-before 'build 'patch-paths
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (string-append (assoc-ref outputs "out")))
                    (share (string-append out "/share/fraggenescan/")))
               (substitute* "run_FragGeneScan.pl"
                 (("system\\(\"rm")
                  (string-append "system(\"" (which "rm")))
                 (("system\\(\"mv")
                  (string-append "system(\"" (which "mv")))
                 (("\\\"awk") (string-append "\"" (which "awk")))
                 ;; This script and other programs expect the training files
                 ;; to be in the non-standard location bin/train/XXX. Change
                 ;; this to be share/fraggenescan/train/XXX instead.
                 (("^\\$train.file = \\$dir.*")
                  (string-append "$train_file = \""
                                 share
                                 "train/\".$FGS_train_file;")))
               (substitute* "run_hmm.c"
                 (("^  strcat\\(train_dir, \\\"train/\\\"\\);")
                  (string-append "  strcpy(train_dir, \"" share "/train/\");"))))
             #t))
         (replace 'build
           (lambda _
             (invoke "make" "clean")
             (invoke "make" "fgs")
             #t))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (string-append (assoc-ref outputs "out")))
                    (bin (string-append out "/bin/"))
                    (share (string-append out "/share/fraggenescan/train")))
               (install-file "run_FragGeneScan.pl" bin)
               (install-file "FragGeneScan" bin)
               (copy-recursively "train" share))
             #t))
         (delete 'check)
         (add-after 'install 'post-install-check
           ;; In lieu of 'make check', run one of the examples and check the
           ;; output files gets created.
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (string-append (assoc-ref outputs "out")))
                    (bin (string-append out "/bin/"))
                    (frag (string-append bin "run_FragGeneScan.pl")))
               ;; Test complete genome.
               (invoke frag
                       "-genome=./example/NC_000913.fna"
                       "-out=./test2"
                       "-complete=1"
                       "-train=complete")
               (unless (and (file-exists? "test2.faa")
                            (file-exists? "test2.ffn")
                            (file-exists? "test2.gff")
                            (file-exists? "test2.out"))
                 (error "Expected files do not exist."))
               ;; Test incomplete sequences.
               (invoke frag
                       "-genome=./example/NC_000913-fgs.ffn"
                       "-out=out"
                       "-complete=0"
                       "-train=454_30")
               #t))))))
    (inputs
     `(("perl" ,perl)
       ("python" ,python-2))) ;not compatible with python 3.
    (home-page "https://sourceforge.net/projects/fraggenescan/")
    (synopsis "Finds potentially fragmented genes in short reads")
    (description
     "FragGeneScan is a program for predicting bacterial and archaeal genes in
short and error-prone DNA sequencing reads.  It can also be applied to predict
genes in incomplete assemblies or complete genomes.")
    ;; GPL3+ according to private correspondense with the authors.
    (license license:gpl3+)))

(define-public fxtract
  (let ((util-commit "776ca85a18a47492af3794745efcb4a905113115"))
    (package
      (name "fxtract")
      (version "2.3")
      (source
       (origin
         (method git-fetch)
         (uri (git-reference
               (url "https://github.com/ctSkennerton/fxtract")
               (commit version)))
         (file-name (git-file-name name version))
         (sha256
          (base32
           "0hab3gpwf4w9s87qlbswq6ws1qqybh4dcqk79q1ahyldzai5fgp5"))))
      (build-system gnu-build-system)
      (arguments
       `(#:make-flags (list
                       (string-append "PREFIX=" (assoc-ref %outputs "out"))
                       "CC=gcc")
         #:test-target "fxtract_test"
         #:phases
         (modify-phases %standard-phases
           (delete 'configure)
           (add-before 'build 'copy-util
             (lambda* (#:key inputs #:allow-other-keys)
               (rmdir "util")
               (copy-recursively (assoc-ref inputs "ctskennerton-util") "util")
               #t))
           ;; Do not use make install as this requires additional dependencies.
           (replace 'install
             (lambda* (#:key outputs #:allow-other-keys)
               (let* ((out (assoc-ref outputs "out"))
                      (bin (string-append out"/bin")))
                 (install-file "fxtract" bin)
                 #t))))))
      (inputs
       `(("pcre" ,pcre)
         ("zlib" ,zlib)))
      (native-inputs
       ;; ctskennerton-util is licensed under GPL2.
       `(("ctskennerton-util"
          ,(origin
             (method git-fetch)
             (uri (git-reference
                   (url "https://github.com/ctSkennerton/util")
                   (commit util-commit)))
             (file-name (string-append
                         "ctstennerton-util-" util-commit "-checkout"))
             (sha256
              (base32
               "0cls1hd4vgj3f36fpzzg4xc77d6f3hpc60cbpfmn2gdr7ykzzad7"))))))
      (home-page "https://github.com/ctSkennerton/fxtract")
      (synopsis "Extract sequences from FASTA and FASTQ files")
      (description
       "Fxtract extracts sequences from a protein or nucleotide fastx (FASTA
or FASTQ) file given a subsequence.  It uses a simple substring search for
basic tasks but can change to using POSIX regular expressions, PCRE, hash
lookups or multi-pattern searching as required.  By default fxtract looks in
the sequence of each record but can also be told to look in the header,
comment or quality sections.")
      ;; 'util' requires SSE instructions.
      (supported-systems '("x86_64-linux"))
      (license license:expat))))

(define-public gemma
  (package
    (name "gemma")
    (version "0.98")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/xiangzhou/GEMMA")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1s3ncnbn45r2hh1cvrqky1kbqq6546biypr4f5mkw1kqlrgyh0yg"))))
    (inputs
     `(("eigen" ,eigen)
       ("gfortran" ,gfortran "lib")
       ("gsl" ,gsl)
       ("lapack" ,lapack)
       ("openblas" ,openblas)
       ("zlib" ,zlib)))
    (build-system gnu-build-system)
    (arguments
     `(#:make-flags
       '(,@(match (%current-system)
         ("x86_64-linux"
          '("FORCE_DYNAMIC=1"))
         ("i686-linux"
          '("FORCE_DYNAMIC=1" "FORCE_32BIT=1"))
         (_
          '("FORCE_DYNAMIC=1" "NO_INTEL_COMPAT=1"))))
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (add-after 'unpack 'find-eigen
           (lambda* (#:key inputs #:allow-other-keys)
             ;; Ensure that Eigen headers can be found
             (setenv "CPLUS_INCLUDE_PATH"
                     (string-append (assoc-ref inputs "eigen")
                                    "/include/eigen3"))
             #t))
         (add-before 'build 'bin-mkdir
          (lambda _
            (mkdir-p "bin")
            #t))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((out (assoc-ref outputs "out")))
               (install-file "bin/gemma"
                             (string-append
                              out "/bin")))
             #t)))
       #:tests? #f)) ; no tests included yet
    (home-page "https://github.com/xiangzhou/GEMMA")
    (synopsis "Tool for genome-wide efficient mixed model association")
    (description
     "Genome-wide Efficient Mixed Model Association (GEMMA) provides a
standard linear mixed model resolver with application in genome-wide
association studies (GWAS).")
    (license license:gpl3)))

(define-public grit
  (package
    (name "grit")
    (version "2.0.5")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/nboley/grit")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1l5v8vfvfbrpmgnrvbrbv40d0arhxcnmxgv2f1mlcqfa3q6bkqm9"))))
    (build-system python-build-system)
    (arguments
     `(#:python ,python-2
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'generate-from-cython-sources
           (lambda* (#:key inputs outputs #:allow-other-keys)
             ;; Delete these C files to force fresh generation from pyx sources.
             (delete-file "grit/sparsify_support_fns.c")
             (delete-file "grit/call_peaks_support_fns.c")
             (substitute* "setup.py"
               (("Cython.Setup") "Cython.Build"))
             #t)))))
    (inputs
     `(("python-scipy" ,python2-scipy)
       ("python-numpy" ,python2-numpy)
       ("python-pysam" ,python2-pysam)
       ("python-networkx" ,python2-networkx)))
    (native-inputs
     `(("python-cython" ,python2-cython)))
    ;; The canonical <http://grit-bio.org> home page times out as of 2020-01-21.
    (home-page "https://github.com/nboley/grit")
    (synopsis "Tool for integrative analysis of RNA-seq type assays")
    (description
     "GRIT is designed to use RNA-seq, TES, and TSS data to build and quantify
full length transcript models.  When none of these data sources are available,
GRIT can be run by providing a candidate set of TES or TSS sites.  In
addition, GRIT can merge in reference junctions and gene boundaries.  GRIT can
also be run in quantification mode, where it uses a provided GTF file and just
estimates transcript expression.")
    (license license:gpl3+)))

(define-public hisat
  (package
    (name "hisat")
    (version "0.1.4")
    (source (origin
              (method url-fetch)
              (uri (string-append
                    "http://ccb.jhu.edu/software/hisat/downloads/hisat-"
                    version "-beta-source.zip"))
              (sha256
               (base32
                "1k381ydranqxp09yf2y7w1d0chz5d59vb6jchi89hbb0prq19lk5"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ;no check target
       #:make-flags '("allall"
                      ;; Disable unsupported `popcnt' instructions on
                      ;; architectures other than x86_64
                      ,@(if (string-prefix? "x86_64"
                                            (or (%current-target-system)
                                                (%current-system)))
                            '()
                            '("POPCNT_CAPABILITY=0")))
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'patch-sources
           (lambda _
             ;; XXX Cannot use snippet because zip files are not supported
             (substitute* "Makefile"
               (("^CC = .*$") "CC = gcc")
               (("^CPP = .*$") "CPP = g++")
               ;; replace BUILD_HOST and BUILD_TIME for deterministic build
               (("-DBUILD_HOST=.*") "-DBUILD_HOST=\"\\\"guix\\\"\"")
               (("-DBUILD_TIME=.*") "-DBUILD_TIME=\"\\\"0\\\"\""))
             (substitute* '("hisat-build" "hisat-inspect")
               (("/usr/bin/env") (which "env")))
             #t))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((bin (string-append (assoc-ref outputs "out") "/bin/")))
               (for-each (lambda (file)
                           (install-file file bin))
                         (find-files
                          "."
                          "hisat(-(build|align|inspect)(-(s|l)(-debug)*)*)*$")))
             #t))
         (delete 'configure))))
    (native-inputs
     `(("unzip" ,unzip)))
    (inputs
     `(("perl" ,perl)
       ("python" ,python)
       ("zlib" ,zlib)))
    ;; Non-portable SSE instructions are used so building fails on platforms
    ;; other than x86_64.
    (supported-systems '("x86_64-linux"))
    (home-page "https://ccb.jhu.edu/software/hisat/index.shtml")
    (synopsis "Hierarchical indexing for spliced alignment of transcripts")
    (description
     "HISAT is a fast and sensitive spliced alignment program for mapping
RNA-seq reads.  In addition to one global FM index that represents a whole
genome, HISAT uses a large set of small FM indexes that collectively cover the
whole genome.  These small indexes (called local indexes) combined with
several alignment strategies enable effective alignment of RNA-seq reads, in
particular, reads spanning multiple exons.")
    (license license:gpl3+)))

(define-public hisat2
  (package
    (name "hisat2")
    (version "2.0.5")
    (source
     (origin
       (method url-fetch)
       (uri (string-append "ftp://ftp.ccb.jhu.edu/pub/infphilo/hisat2"
                           "/downloads/hisat2-" version "-source.zip"))
       (sha256
        (base32
         "0lywnr8kijwsc2aw10dwxic0n0yvip6fl3rjlvc8zzwahamy4x7g"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f                      ; no check target
       #:make-flags (list "CC=gcc" "CXX=g++" "allall")
       #:modules ((guix build gnu-build-system)
                  (guix build utils)
                  (srfi srfi-26))
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'make-deterministic
           (lambda _
             (substitute* "Makefile"
               (("`date`") "0"))
             #t))
         (delete 'configure)
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin/"))
                    (doc (string-append out "/share/doc/hisat2/")))
               (for-each
                (cut install-file <> bin)
                (find-files "."
                            "hisat2(-(build|align|inspect)(-(s|l)(-debug)*)*)*$"))
               (mkdir-p doc)
               (install-file "doc/manual.inc.html" doc))
             #t)))))
    (native-inputs
     `(("unzip" ,unzip)                 ; needed for archive from ftp
       ("perl" ,perl)
       ("pandoc" ,pandoc)))             ; for documentation
    (home-page "https://ccb.jhu.edu/software/hisat2/index.shtml")
    (synopsis "Graph-based alignment of genomic sequencing reads")
    (description "HISAT2 is a fast and sensitive alignment program for mapping
next-generation sequencing reads (both DNA and RNA) to a population of human
genomes (as well as to a single reference genome).  In addition to using one
global @dfn{graph FM} (GFM) index that represents a population of human
genomes, HISAT2 uses a large set of small GFM indexes that collectively cover
the whole genome.  These small indexes, combined with several alignment
strategies, enable rapid and accurate alignment of sequencing reads.  This new
indexing scheme is called a @dfn{Hierarchical Graph FM index} (HGFM).")
    ;; HISAT2 contains files from Bowtie2, which is released under
    ;; GPLv2 or later.  The HISAT2 source files are released under
    ;; GPLv3 or later.
    (license license:gpl3+)))

(define-public hmmer
  (package
    (name "hmmer")
    (version "3.2.1")
    (source
     (origin
       (method url-fetch)
       (uri (string-append
             "http://eddylab.org/software/hmmer/hmmer-" version ".tar.gz"))
       (sha256
        (base32
         "171bivy6xhgjsz5nv53n81pc3frnwz29ylblawk2bv46szwjjqd5"))))
    (build-system gnu-build-system)
    (native-inputs `(("perl" ,perl)))
    (home-page "http://hmmer.org/")
    (synopsis "Biosequence analysis using profile hidden Markov models")
    (description
     "HMMER is used for searching sequence databases for homologs of protein
sequences, and for making protein sequence alignments.  It implements methods
using probabilistic models called profile hidden Markov models (profile
HMMs).")
    ;; hmmer uses non-portable SSE intrinsics so building fails on other
    ;; platforms.
    (supported-systems '("x86_64-linux" "i686-linux"))
    (license license:bsd-3)))

(define-public htseq
  (package
    (name "htseq")
    (version "0.9.1")
    (source (origin
              (method url-fetch)
              (uri (pypi-uri "HTSeq" version))
              (sha256
               (base32
                "11flgb1381xdhk43bzbfm3vhnszkpqg6jk76rpa5xd1zbrvvlnxg"))))
    (build-system python-build-system)
    (native-inputs
     `(("python-cython" ,python-cython)))
    ;; Numpy needs to be propagated when htseq is used as a Python library.
    (propagated-inputs
     `(("python-numpy" ,python-numpy)))
    (inputs
     `(("python-pysam" ,python-pysam)
       ("python-matplotlib" ,python-matplotlib)))
    (home-page "https://htseq.readthedocs.io/")
    (synopsis "Analysing high-throughput sequencing data with Python")
    (description
     "HTSeq is a Python package that provides infrastructure to process data
from high-throughput sequencing assays.")
    (license license:gpl3+)))

(define-public python2-htseq
  (package-with-python2 htseq))

(define-public java-htsjdk
  (package
    (name "java-htsjdk")
    (version "2.3.0") ; last version without build dependency on gradle
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/samtools/htsjdk")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1b178ixcabanm834ydjl3jiakpyxdmki32hqfv2abrzn3rcwa28i"))
              (modules '((guix build utils)))
              (snippet
               ;; Delete pre-built binaries
               '(begin
                  (delete-file-recursively "lib")
                  (mkdir-p "lib")
                  #t))))
    (build-system ant-build-system)
    (arguments
     `(#:tests? #f ; test require Internet access
       #:jdk ,icedtea-8
       #:make-flags
       (list (string-append "-Ddist=" (assoc-ref %outputs "out")
                            "/share/java/htsjdk/"))
       #:build-target "all"
       #:phases
       (modify-phases %standard-phases
         ;; The build phase also installs the jars
         (delete 'install))))
    (inputs
     `(("java-ngs" ,java-ngs)
       ("java-snappy-1" ,java-snappy-1)
       ("java-commons-compress" ,java-commons-compress)
       ("java-commons-logging-minimal" ,java-commons-logging-minimal)
       ("java-commons-jexl-2" ,java-commons-jexl-2)
       ("java-xz" ,java-xz)))
    (native-inputs
     `(("java-testng" ,java-testng)))
    (home-page "http://samtools.github.io/htsjdk/")
    (synopsis "Java API for high-throughput sequencing data (HTS) formats")
    (description
     "HTSJDK is an implementation of a unified Java library for accessing
common file formats, such as SAM and VCF, used for high-throughput
sequencing (HTS) data.  There are also an number of useful utilities for
manipulating HTS data.")
    (license license:expat)))

(define-public java-htsjdk-latest
  (package
    (name "java-htsjdk")
    (version "2.14.3")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/samtools/htsjdk")
                    (commit version)))
              (file-name (string-append name "-" version "-checkout"))
              (sha256
               (base32
                "1lmya1fdjy03mz6zmdmd86j9v9vfhqb3952mqq075navx1i6g4bc"))))
    (build-system ant-build-system)
    (arguments
     `(#:tests? #f                      ; test require Scala
       #:jdk ,icedtea-8
       #:jar-name "htsjdk.jar"
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'remove-useless-build.xml
           (lambda _ (delete-file "build.xml") #t))
         ;; The tests require the scalatest package.
         (add-after 'unpack 'remove-tests
           (lambda _ (delete-file-recursively "src/test") #t)))))
    (inputs
     `(("java-ngs" ,java-ngs)
       ("java-snappy-1" ,java-snappy-1)
       ("java-commons-compress" ,java-commons-compress)
       ("java-commons-logging-minimal" ,java-commons-logging-minimal)
       ("java-commons-jexl-2" ,java-commons-jexl-2)
       ("java-xz" ,java-xz)))
    (native-inputs
     `(("java-junit" ,java-junit)))
    (home-page "http://samtools.github.io/htsjdk/")
    (synopsis "Java API for high-throughput sequencing data (HTS) formats")
    (description
     "HTSJDK is an implementation of a unified Java library for accessing
common file formats, such as SAM and VCF, used for high-throughput
sequencing (HTS) data.  There are also an number of useful utilities for
manipulating HTS data.")
    (license license:expat)))

;; This is needed for picard 2.10.3
(define-public java-htsjdk-2.10.1
  (package (inherit java-htsjdk-latest)
    (name "java-htsjdk")
    (version "2.10.1")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/samtools/htsjdk")
                    (commit version)))
              (file-name (string-append name "-" version "-checkout"))
              (sha256
               (base32
                "1kxh7slm2pm3x9p6jxa1wqsq9a31dhiiflhxnxqcisan4k3rwia2"))))
    (build-system ant-build-system)
    (arguments
     `(#:tests? #f                      ; tests require Scala
       #:jdk ,icedtea-8
       #:jar-name "htsjdk.jar"
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'remove-useless-build.xml
           (lambda _ (delete-file "build.xml") #t))
         ;; The tests require the scalatest package.
         (add-after 'unpack 'remove-tests
           (lambda _ (delete-file-recursively "src/test") #t)))))))

;; This version matches java-htsjdk 2.3.0.  Later versions also require a more
;; recent version of java-htsjdk, which depends on gradle.
(define-public java-picard
  (package
    (name "java-picard")
    (version "2.3.0")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/broadinstitute/picard")
                    (commit version)))
              (file-name (string-append "java-picard-" version "-checkout"))
              (sha256
               (base32
                "1ll7mf4r3by92w2nhlmpa591xd1f46xlkwh59mq6fvbb5pdwzvx6"))
              (modules '((guix build utils)))
              (snippet
               '(begin
                  ;; Delete pre-built binaries.
                  (delete-file-recursively "lib")
                  (mkdir-p "lib")
                  (substitute* "build.xml"
                    ;; Remove build-time dependency on git.
                    (("failifexecutionfails=\"true\"")
                     "failifexecutionfails=\"false\"")
                    ;; Use our htsjdk.
                    (("depends=\"compile-htsjdk, ")
                     "depends=\"")
                    (("depends=\"compile-htsjdk-tests, ")
                     "depends=\"")
                    ;; Build picard-lib.jar before building picard.jar
                    (("name=\"picard-jar\" depends=\"" line)
                     (string-append line "picard-lib-jar, ")))
                  #t))))
    (build-system ant-build-system)
    (arguments
     `(#:build-target "picard-jar"
       #:test-target "test"
       ;; Tests require jacoco:coverage.
       #:tests? #f
       #:make-flags
       (list (string-append "-Dhtsjdk_lib_dir="
                            (assoc-ref %build-inputs "java-htsjdk")
                            "/share/java/htsjdk/")
             "-Dhtsjdk-classes=dist/tmp"
             (string-append "-Dhtsjdk-version="
                            ,(package-version java-htsjdk)))
       #:jdk ,icedtea-8
       #:phases
       (modify-phases %standard-phases
         ;; FIXME: this phase fails with "duplicate entry: htsjdk/samtools/AbstractBAMFileIndex$1.class"
         (delete 'generate-jar-indices)
         (add-after 'unpack 'use-our-htsjdk
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* "build.xml"
               (("\\$\\{htsjdk\\}/lib")
                (string-append (assoc-ref inputs "java-htsjdk")
                               "/share/java/htsjdk/")))
             #t))
         (add-after 'unpack 'make-test-target-independent
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* "build.xml"
               (("name=\"test\" depends=\"compile, ")
                "name=\"test\" depends=\""))
             #t))
         (replace 'install (install-jars "dist")))))
    (inputs
     `(("java-htsjdk" ,java-htsjdk)
       ("java-guava" ,java-guava)))
    (native-inputs
     `(("java-testng" ,java-testng)))
    (home-page "http://broadinstitute.github.io/picard/")
    (synopsis "Tools for manipulating high-throughput sequencing data and formats")
    (description "Picard is a set of Java command line tools for manipulating
high-throughput sequencing (HTS) data and formats.  Picard is implemented
using the HTSJDK Java library to support accessing file formats that are
commonly used for high-throughput sequencing data such as SAM, BAM, CRAM and
VCF.")
    (license license:expat)))

;; This is needed for dropseq-tools
(define-public java-picard-2.10.3
  (package
    (name "java-picard")
    (version "2.10.3")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/broadinstitute/picard")
                    (commit version)))
              (file-name (string-append "java-picard-" version "-checkout"))
              (sha256
               (base32
                "1ajlx31l6i1k3y2rhnmgq07sz99g2czqfqgkr9mihmdjp3gwjhvi"))))
    (build-system ant-build-system)
    (arguments
     `(#:jar-name "picard.jar"
       ;; Tests require jacoco:coverage.
       #:tests? #f
       #:jdk ,icedtea-8
       #:main-class "picard.cmdline.PicardCommandLine"
       #:modules ((guix build ant-build-system)
                  (guix build utils)
                  (guix build java-utils)
                  (sxml simple)
                  (sxml transform)
                  (sxml xpath))
       #:phases
       (modify-phases %standard-phases
         ;; FIXME: this phase fails with "duplicate entry: htsjdk/samtools/AbstractBAMFileIndex$1.class"
         (delete 'generate-jar-indices)
         (add-after 'unpack 'remove-useless-build.xml
           (lambda _ (delete-file "build.xml") #t))
         ;; This is necessary to ensure that htsjdk is found when using
         ;; picard.jar as an executable.
         (add-before 'build 'edit-classpath-in-manifest
           (lambda* (#:key inputs #:allow-other-keys)
             (chmod "build.xml" #o664)
             (call-with-output-file "build.xml.new"
               (lambda (port)
                 (sxml->xml
                  (pre-post-order
                   (with-input-from-file "build.xml"
                     (lambda _ (xml->sxml #:trim-whitespace? #t)))
                   `((target    . ,(lambda (tag . kids)
                                     (let ((name ((sxpath '(name *text*))
                                                  (car kids)))
                                           ;; FIXME: We're breaking the line
                                           ;; early with a dummy path to
                                           ;; ensure that the store reference
                                           ;; isn't broken apart and can still
                                           ;; be found by the reference
                                           ;; scanner.
                                           (msg (format #f
                                                        "\
Class-Path: /~a \
 ~a/share/java/htsjdk.jar${line.separator}${line.separator}"
                                                        ;; maximum line length is 70
                                                        (string-tabulate (const #\b) 57)
                                                        (assoc-ref inputs "java-htsjdk"))))
                                       (if (member "manifest" name)
                                           `(,tag ,@kids
                                                  (replaceregexp
                                                   (@ (file "${manifest.file}")
                                                      (match "\\r\\n\\r\\n")
                                                      (replace "${line.separator}")))
                                                  (echo
                                                   (@ (message ,msg)
                                                      (file "${manifest.file}")
                                                      (append "true"))))
                                           `(,tag ,@kids)))))
                     (*default* . ,(lambda (tag . kids) `(,tag ,@kids)))
                     (*text*    . ,(lambda (_ txt) txt))))
                  port)))
             (rename-file "build.xml.new" "build.xml")
             #t)))))
    (propagated-inputs
     `(("java-htsjdk" ,java-htsjdk-2.10.1)))
    (native-inputs
     `(("java-testng" ,java-testng)
       ("java-guava" ,java-guava)))
    (home-page "http://broadinstitute.github.io/picard/")
    (synopsis "Tools for manipulating high-throughput sequencing data and formats")
    (description "Picard is a set of Java command line tools for manipulating
high-throughput sequencing (HTS) data and formats.  Picard is implemented
using the HTSJDK Java library to support accessing file formats that are
commonly used for high-throughput sequencing data such as SAM, BAM, CRAM and
VCF.")
    (license license:expat)))

;; This is the last version of Picard to provide net.sf.samtools
(define-public java-picard-1.113
  (package (inherit java-picard)
    (name "java-picard")
    (version "1.113")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/broadinstitute/picard")
                    (commit version)))
              (file-name (string-append "java-picard-" version "-checkout"))
              (sha256
               (base32
                "0lkpvin2fz3hhly4l02kk56fqy8lmlgyzr9kmvljk6ry6l1hw973"))
              (modules '((guix build utils)))
              (snippet
               '(begin
                  ;; Delete pre-built binaries.
                  (delete-file-recursively "lib")
                  (mkdir-p "lib")
                  #t))))
    (build-system ant-build-system)
    (arguments
     `(#:build-target "picard-jar"
       #:test-target "test"
       ;; FIXME: the class path at test time is wrong.
       ;; [testng] Error: A JNI error has occurred, please check your installation and try again
       ;; [testng] Exception in thread "main" java.lang.NoClassDefFoundError: com/beust/jcommander/ParameterException
       #:tests? #f
       #:jdk ,icedtea-8
       ;; This is only used for tests.
       #:make-flags
       (list "-Dsamjdk.intel_deflater_so_path=lib/jni/libIntelDeflater.so")
       #:phases
       (modify-phases %standard-phases
         ;; FIXME: This phase fails.
         (delete 'generate-jar-indices)
         ;; Do not use bundled ant bzip2.
         (add-after 'unpack 'use-ant-bzip
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* "build.xml"
               (("\\$\\{lib\\}/apache-ant-1.8.2-bzip2.jar")
                (string-append (assoc-ref inputs "ant")
                               "/lib/ant.jar")))
             #t))
         (add-after 'unpack 'make-test-target-independent
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* "build.xml"
               (("name=\"test\" depends=\"compile, ")
                "name=\"test\" depends=\"compile-tests, ")
               (("name=\"compile\" depends=\"compile-src, compile-tests\"")
                "name=\"compile\" depends=\"compile-src\""))
             #t))
         (add-after 'unpack 'fix-deflater-path
           (lambda* (#:key outputs #:allow-other-keys)
             (substitute* "src/java/net/sf/samtools/Defaults.java"
               (("getStringProperty\\(\"intel_deflater_so_path\", null\\)")
                (string-append "getStringProperty(\"intel_deflater_so_path\", \""
                               (assoc-ref outputs "out")
                               "/lib/jni/libIntelDeflater.so"
                               "\")")))
             #t))
         ;; Build the deflater library, because we've previously deleted the
         ;; pre-built one.  This can only be built with access to the JDK
         ;; sources.
         (add-after 'build 'build-jni
           (lambda* (#:key inputs #:allow-other-keys)
             (mkdir-p "lib/jni")
             (mkdir-p "jdk-src")
             (invoke "tar" "--strip-components=1" "-C" "jdk-src"
                     "-xf" (assoc-ref inputs "jdk-src"))
             (invoke "javah" "-jni"
                     "-classpath" "classes"
                     "-d" "lib/"
                     "net.sf.samtools.util.zip.IntelDeflater")
             (with-directory-excursion "src/c/inteldeflater"
               (invoke "gcc" "-I../../../lib" "-I."
                       (string-append "-I" (assoc-ref inputs "jdk")
                                      "/include/linux")
                       "-I../../../jdk-src/src/share/native/common/"
                       "-I../../../jdk-src/src/solaris/native/common/"
                       "-c" "-O3" "-fPIC" "IntelDeflater.c")
               (invoke "gcc" "-shared"
                       "-o" "../../../lib/jni/libIntelDeflater.so"
                       "IntelDeflater.o" "-lz" "-lstdc++"))
             #t))
         ;; We can only build everything else after building the JNI library.
         (add-after 'build-jni 'build-rest
           (lambda* (#:key make-flags #:allow-other-keys)
             (apply invoke `("ant" "all" ,@make-flags))
             #t))
         (add-before 'build 'set-JAVA6_HOME
           (lambda _
             (setenv "JAVA6_HOME" (getenv "JAVA_HOME"))
             #t))
         (replace 'install (install-jars "dist"))
         (add-after 'install 'install-jni-lib
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((jni (string-append (assoc-ref outputs "out")
                                       "/lib/jni")))
               (mkdir-p jni)
               (install-file "lib/jni/libIntelDeflater.so" jni)
               #t))))))
    (inputs
     `(("java-snappy-1" ,java-snappy-1)
       ("java-commons-jexl-2" ,java-commons-jexl-2)
       ("java-cofoja" ,java-cofoja)
       ("ant" ,ant) ; for bzip2 support at runtime
       ("zlib" ,zlib)))
    (native-inputs
     `(("ant-apache-bcel" ,ant-apache-bcel)
       ("ant-junit" ,ant-junit)
       ("java-testng" ,java-testng)
       ("java-commons-bcel" ,java-commons-bcel)
       ("java-jcommander" ,java-jcommander)
       ("jdk" ,icedtea-8 "jdk")
       ("jdk-src" ,(car (assoc-ref (package-native-inputs icedtea-8) "jdk-drop")))))))

(define-public fastqc
  (package
    (name "fastqc")
    (version "0.11.5")
    (source
     (origin
       (method url-fetch)
       (uri (string-append "http://www.bioinformatics.babraham.ac.uk/"
                           "projects/fastqc/fastqc_v"
                           version "_source.zip"))
       (sha256
        (base32
         "18rrlkhcrxvvvlapch4dpj6xc6mpayzys8qfppybi8jrpgx5cc5f"))))
    (build-system ant-build-system)
    (arguments
     `(#:tests? #f                      ; there are no tests
       #:build-target "build"
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'fix-dependencies
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* "build.xml"
               (("jbzip2-0.9.jar")
                (string-append (assoc-ref inputs "java-jbzip2")
                               "/share/java/jbzip2.jar"))
               (("sam-1.103.jar")
                (string-append (assoc-ref inputs "java-picard-1.113")
                               "/share/java/sam-1.112.jar"))
               (("cisd-jhdf5.jar")
                (string-append (assoc-ref inputs "java-cisd-jhdf5")
                               "/share/java/sis-jhdf5.jar")))
             #t))
         ;; There is no installation target
         (replace 'install
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let* ((out   (assoc-ref outputs "out"))
                    (bin   (string-append out "/bin"))
                    (share (string-append out "/share/fastqc/"))
                    (exe   (string-append share "/fastqc")))
               (for-each mkdir-p (list bin share))
               (copy-recursively "bin" share)
               (substitute* exe
                 (("my \\$java_bin = 'java';")
                  (string-append "my $java_bin = '"
                                 (assoc-ref inputs "java")
                                 "/bin/java';")))
               (chmod exe #o555)
               (symlink exe (string-append bin "/fastqc"))
               #t))))))
    (inputs
     `(("java" ,icedtea)
       ("perl" ,perl)                   ; needed for the wrapper script
       ("java-cisd-jhdf5" ,java-cisd-jhdf5)
       ("java-picard-1.113" ,java-picard-1.113)
       ("java-jbzip2" ,java-jbzip2)))
    (native-inputs
     `(("unzip" ,unzip)))
    (home-page "https://www.bioinformatics.babraham.ac.uk/projects/fastqc/")
    (synopsis "Quality control tool for high throughput sequence data")
    (description
     "FastQC aims to provide a simple way to do some quality control
checks on raw sequence data coming from high throughput sequencing
pipelines.  It provides a modular set of analyses which you can use to
give a quick impression of whether your data has any problems of which
you should be aware before doing any further analysis.

The main functions of FastQC are:

@itemize
@item Import of data from BAM, SAM or FastQ files (any variant);
@item Providing a quick overview to tell you in which areas there may
  be problems;
@item Summary graphs and tables to quickly assess your data;
@item Export of results to an HTML based permanent report;
@item Offline operation to allow automated generation of reports
  without running the interactive application.
@end itemize\n")
    (license license:gpl3+)))

(define-public fastp
  (package
    (name "fastp")
    (version "0.14.1")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/OpenGene/fastp")
             (commit (string-append "v" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "1r6ms5zbf5rps4rgp4z73nczadl00b5rqylw8f684isfz27dp0xh"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; there are none
       #:make-flags
       (list (string-append "BINDIR=" (assoc-ref %outputs "out") "/bin"))
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (add-before 'install 'create-target-dir
           (lambda* (#:key outputs #:allow-other-keys)
             (mkdir-p (string-append (assoc-ref outputs "out") "/bin"))
             #t)))))
    (inputs
     `(("zlib" ,zlib)))
    (home-page "https://github.com/OpenGene/fastp/")
    (synopsis "All-in-one FastQ preprocessor")
    (description
     "Fastp is a tool designed to provide fast all-in-one preprocessing for
FastQ files.  This tool has multi-threading support to afford high
performance.")
    (license license:expat)))

(define-public htslib
  (package
    (name "htslib")
    (version "1.11")
    (source (origin
              (method url-fetch)
              (uri (string-append
                    "https://github.com/samtools/htslib/releases/download/"
                    version "/htslib-" version ".tar.bz2"))
              (sha256
               (base32
                "1mrq4mihzx37yqhj3sfz6da6mw49niia808bzsw2gkkgmadxvyng"))))
    (build-system gnu-build-system)
    ;; Let htslib translate "gs://" and "s3://" to regular https links with
    ;; "--enable-gcs" and "--enable-s3". For these options to work, we also
    ;; need to set "--enable-libcurl".
    (arguments
     `(#:configure-flags '("--enable-gcs"
                           "--enable-libcurl"
                           "--enable-s3")))
    (inputs
     `(("curl" ,curl)
       ("openssl" ,openssl)))
    ;; This is referred to in the pkg-config file as a required library.
    (propagated-inputs
     `(("zlib" ,zlib)))
    (native-inputs
     `(("perl" ,perl)))
    (home-page "https://www.htslib.org")
    (synopsis "C library for reading/writing high-throughput sequencing data")
    (description
     "HTSlib is a C library for reading/writing high-throughput sequencing
data.  It also provides the @command{bgzip}, @command{htsfile}, and
@command{tabix} utilities.")
    ;; Files under cram/ are released under the modified BSD license;
    ;; the rest is released under the Expat license
    (license (list license:expat license:bsd-3))))

(define-public htslib-1.9
  (package (inherit htslib)
    (name "htslib")
    (version "1.9")
    (source (origin
              (method url-fetch)
              (uri (string-append
                    "https://github.com/samtools/htslib/releases/download/"
                    version "/htslib-" version ".tar.bz2"))
              (sha256
               (base32
                "16ljv43sc3fxmv63w7b2ff8m1s7h89xhazwmbm1bicz8axq8fjz0"))))))

;; This package should be removed once no packages rely upon it.
(define htslib-1.3
  (package
    (inherit htslib)
    (version "1.3.1")
    (source (origin
              (method url-fetch)
              (uri (string-append
                    "https://github.com/samtools/htslib/releases/download/"
                    version "/htslib-" version ".tar.bz2"))
              (sha256
               (base32
                "1rja282fwdc25ql6izkhdyh8ppw8x2fs0w0js78zgkmqjlikmma9"))))))

(define-public idr
  (package
    (name "idr")
    (version "2.0.3")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/nboley/idr")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "04j876h6z444v2q79drxx283d3k5snd72kj895wbalnl42206x9g"))
              ;; Delete generated C code.
              (snippet
               '(begin (delete-file "idr/inv_cdf.c") #t))))
    (build-system python-build-system)
    ;; There is only one test ("test_inv_cdf.py") and it tests features that
    ;; are no longer part of this package.  It also asserts False, which
    ;; causes the tests to always fail.
    (arguments `(#:tests? #f))
    (propagated-inputs
     `(("python-scipy" ,python-scipy)
       ("python-sympy" ,python-sympy)
       ("python-numpy" ,python-numpy)
       ("python-matplotlib" ,python-matplotlib)))
    (native-inputs
     `(("python-cython" ,python-cython)))
    (home-page "https://github.com/nboley/idr")
    (synopsis "Tool to measure the irreproducible discovery rate (IDR)")
    (description
     "The IDR (Irreproducible Discovery Rate) framework is a unified approach
to measure the reproducibility of findings identified from replicate
experiments and provide highly stable thresholds based on reproducibility.")
    (license license:gpl2+)))

(define-public jellyfish
  (package
    (name "jellyfish")
    (version "2.2.10")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://github.com/gmarcais/Jellyfish/"
                                  "releases/download/v" version
                                  "/jellyfish-" version ".tar.gz"))
              (sha256
               (base32
                "1k4pc3fvv6w1km2yph4m5sd78fbxp21d6xyzgmy0gjihzc6mb249"))))
    (build-system gnu-build-system)
    (outputs '("out"      ;for library
               "ruby"     ;for Ruby bindings
               "python")) ;for Python bindings
    (arguments
     `(#:configure-flags
       (list (string-append "--enable-ruby-binding="
                            (assoc-ref %outputs "ruby"))
             (string-append "--enable-python-binding="
                            (assoc-ref %outputs "python")))
       #:phases
       (modify-phases %standard-phases
         (add-before 'check 'set-SHELL-variable
           (lambda _
             ;; generator_manager.hpp either uses /bin/sh or $SHELL
             ;; to run tests.
             (setenv "SHELL" (which "bash"))
             #t)))))
    (native-inputs
     `(("bc" ,bc)
       ("time" ,time)
       ("ruby" ,ruby)
       ("python" ,python-2)
       ("pkg-config" ,pkg-config)))
    (inputs
     `(("htslib" ,htslib)))
    (synopsis "Tool for fast counting of k-mers in DNA")
    (description
     "Jellyfish is a tool for fast, memory-efficient counting of k-mers in
DNA.  A k-mer is a substring of length k, and counting the occurrences of all
such substrings is a central step in many analyses of DNA sequence.  Jellyfish
is a command-line program that reads FASTA and multi-FASTA files containing
DNA sequences.  It outputs its k-mer counts in a binary format, which can be
translated into a human-readable text format using the @code{jellyfish dump}
command, or queried for specific k-mers with @code{jellyfish query}.")
    (home-page "http://www.genome.umd.edu/jellyfish.html")
    ;; JELLYFISH seems to be 64-bit only.
    (supported-systems '("x86_64-linux" "aarch64-linux" "mips64el-linux"))
    ;; The combined work is published under the GPLv3 or later.  Individual
    ;; files such as lib/jsoncpp.cpp are released under the Expat license.
    (license (list license:gpl3+ license:expat))))

(define-public khmer
  (package
    (name "khmer")
    (version "3.0.0a3")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/dib-lab/khmer")
             (commit (string-append "v" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "01l4jczglkl7yfhgvzx8j0df7k54bk1r8sli9ll16i1mis0d8f37"))
       (modules '((guix build utils)))
       (snippet
        '(begin
           ;; Delete bundled libraries.  We do not replace the bundled seqan
           ;; as it is a modified subset of the old version 1.4.1.
           ;;
           ;; We do not replace the bundled MurmurHash as the canonical
           ;; repository for this code 'SMHasher' is unsuitable for providing
           ;; a library.  See
           ;; https://lists.gnu.org/archive/html/guix-devel/2016-06/msg00977.html
           (delete-file-recursively "third-party/zlib")
           (delete-file-recursively "third-party/bzip2")
           (delete-file-recursively "third-party/seqan")
           (substitute* "setup.cfg"
             (("# libraries = z,bz2")
              "libraries = z,bz2")
             (("include:third-party/zlib:third-party/bzip2")
              "include:"))
           #t))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'set-cc
           (lambda _ (setenv "CC" "gcc") #t))

         (add-before 'reset-gzip-timestamps 'make-files-writable
           (lambda* (#:key outputs #:allow-other-keys)
             ;; Make sure .gz files are writable so that the
             ;; 'reset-gzip-timestamps' phase can do its work.
             (let ((out (assoc-ref outputs "out")))
               (for-each make-file-writable
                         (find-files out "\\.gz$"))
               #t))))))
    (native-inputs
     `(("python-cython" ,python-cython)
       ("python-pytest" ,python-pytest)
       ("python-pytest-runner" ,python-pytest-runner)))
    (inputs
     `(("zlib" ,zlib)
       ("bzip2" ,bzip2)
       ("seqan" ,seqan-1)
       ("python-screed" ,python-screed)
       ("python-bz2file" ,python-bz2file)))
    (home-page "https://khmer.readthedocs.org/")
    (synopsis "K-mer counting, filtering and graph traversal library")
    (description "The khmer software is a set of command-line tools for
working with DNA shotgun sequencing data from genomes, transcriptomes,
metagenomes and single cells.  Khmer can make de novo assemblies faster, and
sometimes better.  Khmer can also identify and fix problems with shotgun
data.")
    ;; When building on i686, armhf and mips64el, we get the following error:
    ;; error: ['khmer', 'khmer.tests', 'oxli'] require 64-bit operating system
    (supported-systems '("x86_64-linux" "aarch64-linux"))
    (license license:bsd-3)))

(define-public kaiju
  (package
    (name "kaiju")
    (version "1.6.3")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/bioinformatics-centre/kaiju")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "119pzi0ddzv9mjg4wwa6han0cwr3k3ssn7kirvsjfcq05mi5ka0x"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; There are no tests.
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (add-before 'build 'move-to-src-dir
           (lambda _ (chdir "src") #t))
         (replace 'install
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let ((bin (string-append (assoc-ref outputs "out") "/bin")))
               (mkdir-p bin)
               (chdir "..")
               (copy-recursively "bin" bin))
             #t)))))
    (inputs
     `(("perl" ,perl)
       ("zlib" ,zlib)))
    (home-page "http://kaiju.binf.ku.dk/")
    (synopsis "Fast and sensitive taxonomic classification for metagenomics")
    (description "Kaiju is a program for sensitive taxonomic classification
of high-throughput sequencing reads from metagenomic whole genome sequencing
experiments.")
    (license license:gpl3+)))

(define-public macs
  (package
    (name "macs")
    (version "2.2.6")
    (source (origin
              ;; The PyPi tarball does not contain tests.
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/macs3-project/MACS")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1c5gxr0mk6hkd4vclf0k00wvyvzw2vrmk52c85338p7aqjwg6n15"))
        (modules '((guix build utils)))
        ;; Remove files generated by Cython
        (snippet
         '(begin
            (for-each (lambda (file)
                        (let ((generated-file
                                (string-append (string-drop-right file 3) "c")))
                          (when (file-exists? generated-file)
                            (delete-file generated-file))))
                      (find-files "." "\\.pyx$"))
            (delete-file "MACS2/IO/CallPeakUnitPrecompiled.c")
            #t))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (replace 'check
           (lambda* (#:key tests? inputs outputs #:allow-other-keys)
             (when tests?
               (add-installed-pythonpath inputs outputs)
               (invoke "pytest" "-v"))
             #t)))))
    (inputs
     `(("python-numpy" ,python-numpy)))
    (native-inputs
     `(("python-cython" ,python-cython)
       ("python-pytest" ,python-pytest)))
    (home-page "https://github.com/macs3-project/MACS")
    (synopsis "Model based analysis for ChIP-Seq data")
    (description
     "MACS is an implementation of a ChIP-Seq analysis algorithm for
identifying transcript factor binding sites named Model-based Analysis of
ChIP-Seq (MACS).  MACS captures the influence of genome complexity to evaluate
the significance of enriched ChIP regions and it improves the spatial
resolution of binding sites through combining the information of both
sequencing tag position and orientation.")
    (license license:bsd-3)))

(define-public mafft
  (package
    (name "mafft")
    (version "7.471")
    (source (origin
              (method url-fetch)
              (uri (string-append
                    "https://mafft.cbrc.jp/alignment/software/mafft-" version
                    "-without-extensions-src.tgz"))
              (file-name (string-append name "-" version ".tgz"))
              (sha256
               (base32
                "0r1973fx2scq4712zdqfy67wkzqj0c0bhrdy4jxhvq40mdxyry30"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; no automated tests, though there are tests in the read me
       #:make-flags (let ((out (assoc-ref %outputs "out")))
                      (list (string-append "PREFIX=" out)
                            (string-append "BINDIR="
                                           (string-append out "/bin"))))
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'enter-dir
           (lambda _ (chdir "core") #t))
         (add-after 'enter-dir 'patch-makefile
           (lambda _
             ;; on advice from the MAFFT authors, there is no need to
             ;; distribute mafft-profile, mafft-distance, or
             ;; mafft-homologs.rb as they are too "specialised".
             (substitute* "Makefile"
               ;; remove mafft-homologs.rb from SCRIPTS
               (("^SCRIPTS = mafft mafft-homologs.rb")
                "SCRIPTS = mafft")
               ;; remove mafft-homologs from MANPAGES
               (("^MANPAGES = mafft.1 mafft-homologs.1")
                "MANPAGES = mafft.1")
               ;; remove mafft-distance from PROGS
               (("^PROGS = dvtditr dndfast7 dndblast sextet5 mafft-distance")
                "PROGS = dvtditr dndfast7 dndblast sextet5")
               ;; remove mafft-profile from PROGS
               (("splittbfast disttbfast tbfast mafft-profile 2cl mccaskillwrap")
                "splittbfast disttbfast tbfast f2cl mccaskillwrap")
               (("^rm -f mafft-profile mafft-profile.exe") "#")
               (("^rm -f mafft-distance mafft-distance.exe") ")#")
               ;; do not install MAN pages in libexec folder
               (("^\t\\$\\(INSTALL\\) -m 644 \\$\\(MANPAGES\\) \
\\$\\(DESTDIR\\)\\$\\(LIBDIR\\)") "#"))
             #t))
         (add-after 'enter-dir 'patch-paths
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* '("pairash.c"
                            "mafft.tmpl")
               (("perl") (which "perl"))
               (("([\"`| ])awk" _ prefix)
                (string-append prefix (which "awk")))
               (("grep") (which "grep")))
             #t))
         (delete 'configure)
         (add-after 'install 'wrap-programs
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin"))
                    (path (string-append
                           (assoc-ref %build-inputs "coreutils") "/bin:")))
               (for-each (lambda (file)
                           (wrap-program file
                             `("PATH" ":" prefix (,path))))
                         (find-files bin)))
             #t)))))
    (inputs
     `(("perl" ,perl)
       ("ruby" ,ruby)
       ("gawk" ,gawk)
       ("grep" ,grep)
       ("coreutils" ,coreutils)))
    (home-page "https://mafft.cbrc.jp/alignment/software/")
    (synopsis "Multiple sequence alignment program")
    (description
     "MAFFT offers a range of multiple alignment methods for nucleotide and
protein sequences.  For instance, it offers L-INS-i (accurate; for alignment
of <~200 sequences) and FFT-NS-2 (fast; for alignment of <~30,000
sequences).")
    (license (license:non-copyleft
              "https://mafft.cbrc.jp/alignment/software/license.txt"
              "BSD-3 with different formatting"))))

(define-public mash
  (package
    (name "mash")
    (version "2.1")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/marbl/mash")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "049hwcc059p2fd9vwndn63laifvvsi0wmv84i6y1fr79k15dxwy6"))
              (modules '((guix build utils)))
              (snippet
               '(begin
                  ;; Delete bundled kseq.
                  ;; TODO: Also delete bundled murmurhash and open bloom filter.
                  (delete-file "src/mash/kseq.h")
                  #t))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; No tests.
       #:configure-flags
       (list
        (string-append "--with-capnp=" (assoc-ref %build-inputs "capnproto"))
        (string-append "--with-gsl=" (assoc-ref %build-inputs "gsl")))
       #:make-flags (list "CC=gcc")
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'fix-includes
           (lambda _
             (substitute* '("src/mash/Sketch.cpp"
                            "src/mash/CommandFind.cpp"
                            "src/mash/CommandScreen.cpp")
               (("^#include \"kseq\\.h\"")
                "#include \"htslib/kseq.h\""))
             #t))
         (add-after 'fix-includes 'use-c++14
           (lambda _
             ;; capnproto 0.7 requires c++14 to build
             (substitute* "configure.ac"
               (("c\\+\\+11") "c++14"))
             (substitute* "Makefile.in"
               (("c\\+\\+11") "c++14"))
             #t)))))
    (native-inputs
     `(("autoconf" ,autoconf)
       ;; Capnproto and htslib are statically embedded in the final
       ;; application. Therefore we also list their licenses, below.
       ("capnproto" ,capnproto)
       ("htslib" ,htslib)))
    (inputs
     `(("gsl" ,gsl)
       ("zlib" ,zlib)))
    (supported-systems '("x86_64-linux"))
    (home-page "https://mash.readthedocs.io")
    (synopsis "Fast genome and metagenome distance estimation using MinHash")
    (description "Mash is a fast sequence distance estimator that uses the
MinHash algorithm and is designed to work with genomes and metagenomes in the
form of assemblies or reads.")
    (license (list license:bsd-3          ; Mash
                   license:expat          ; HTSlib and capnproto
                   license:public-domain  ; MurmurHash 3
                   license:cpl1.0))))     ; Open Bloom Filter

(define-public metabat
  (package
    (name "metabat")
    (version "2.12.1")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://bitbucket.org/berkeleylab/metabat.git")
             (commit (string-append "v" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "0hyg2smw1nz69mfvjpk45xyyychmda92c80a0cv7baji84ri4iyn"))
       (patches (search-patches "metabat-fix-compilation.patch"))))
    (build-system scons-build-system)
    (arguments
     `(#:scons ,scons-python2
       #:scons-flags
       (list (string-append "PREFIX=" (assoc-ref %outputs "out"))
             (string-append "BOOST_ROOT=" (assoc-ref %build-inputs "boost")))
       #:tests? #f ;; Tests are run during the build phase.
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'fix-includes
           (lambda _
             (substitute* "src/BamUtils.h"
               (("^#include \"bam/bam\\.h\"")
                "#include \"samtools/bam.h\"")
               (("^#include \"bam/sam\\.h\"")
                "#include \"samtools/sam.h\""))
             (substitute* "src/KseqReader.h"
               (("^#include \"bam/kseq\\.h\"")
                "#include \"htslib/kseq.h\""))
             #t))
         (add-after 'unpack 'fix-scons
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* "SConstruct"
               (("^htslib_dir += 'samtools'")
                (string-append "htslib_dir = '"
                               (assoc-ref inputs "htslib")
                               "'"))
               (("^samtools_dir = 'samtools'")
                (string-append "samtools_dir = '"
                               (assoc-ref inputs "samtools")
                               "'"))
               (("^findStaticOrShared\\('bam', hts_lib")
                (string-append "findStaticOrShared('bam', '"
                               (assoc-ref inputs "samtools")
                               "/lib'"))
               ;; Do not distribute README.
               (("^env\\.Install\\(idir_prefix, 'README\\.md'\\)") ""))
             #t)))))
    (inputs
     `(("zlib" ,zlib)
       ("perl" ,perl)
       ("samtools" ,samtools)
       ("htslib" ,htslib)
       ("boost" ,boost)))
    (home-page "https://bitbucket.org/berkeleylab/metabat")
    (synopsis
     "Reconstruction of single genomes from complex microbial communities")
    (description
     "Grouping large genomic fragments assembled from shotgun metagenomic
sequences to deconvolute complex microbial communities, or metagenome binning,
enables the study of individual organisms and their interactions.  MetaBAT is
an automated metagenome binning software, which integrates empirical
probabilistic distances of genome abundance and tetranucleotide frequency.")
    ;; The source code contains inline assembly.
    (supported-systems '("x86_64-linux" "i686-linux"))
    (license (license:non-copyleft "file://license.txt"
                                   "See license.txt in the distribution."))))

(define-public minced
  (package
    (name "minced")
    (version "0.3.2")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/ctSkennerton/minced")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1f5h9him0gd355cnx7p6pnxpknhckd4g0v62mg8zyhfbx9as25fv"))))
    (build-system gnu-build-system)
    (arguments
     `(#:test-target "test"
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (add-before 'check 'fix-test
           (lambda _
             ;; Fix test for latest version.
             (substitute* "t/Aquifex_aeolicus_VF5.expected"
               (("minced:0.1.6") "minced:0.2.0"))
             #t))
         (replace 'install ; No install target.
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin"))
                    (wrapper (string-append bin "/minced")))
               ;; Minced comes with a wrapper script that tries to figure out where
               ;; it is located before running the JAR. Since these paths are known
               ;; to us, we build our own wrapper to avoid coreutils dependency.
               (install-file "minced.jar" bin)
               (with-output-to-file wrapper
                 (lambda _
                   (display
                    (string-append
                     "#!" (assoc-ref inputs "bash") "/bin/sh\n\n"
                     (assoc-ref inputs "jre") "/bin/java -jar "
                     bin "/minced.jar \"$@\"\n"))))
               (chmod wrapper #o555))
             #t)))))
    (native-inputs
     `(("jdk" ,icedtea "jdk")))
    (inputs
     `(("bash" ,bash)
       ("jre" ,icedtea "out")))
    (home-page "https://github.com/ctSkennerton/minced")
    (synopsis "Mining CRISPRs in Environmental Datasets")
    (description
     "MinCED is a program to find Clustered Regularly Interspaced Short
Palindromic Repeats (CRISPRs) in DNA sequences.  It can be used for
unassembled metagenomic reads, but is mainly designed for full genomes and
assembled metagenomic sequence.")
    (license license:gpl3+)))

(define-public miso
  (package
    (name "miso")
    (version "0.5.4")
    (source (origin
              (method url-fetch)
              (uri (pypi-uri "misopy" version))
              (sha256
               (base32
                "1z3x0vd8ma7pdrnywj7i3kgwl89sdkwrrn62zl7r5calqaq2hyip"))
              (modules '((guix build utils)))
              (snippet '(begin
                          (substitute* "setup.py"
                            ;; Use setuptools, or else the executables are not
                            ;; installed.
                            (("distutils.core") "setuptools")
                            ;; Use "gcc" instead of "cc" for compilation.
                            (("^defines")
                             "cc.set_executables(
compiler='gcc',
compiler_so='gcc',
linker_exe='gcc',
linker_so='gcc -shared'); defines"))
                          #t))))
    (build-system python-build-system)
    (arguments
     `(#:python ,python-2               ; only Python 2 is supported
       #:tests? #f))                    ; no "test" target
    (inputs
     `(("samtools" ,samtools)
       ("python-numpy" ,python2-numpy)
       ("python-pysam" ,python2-pysam)
       ("python-scipy" ,python2-scipy)
       ("python-matplotlib" ,python2-matplotlib)))
    (native-inputs
     `(("python-mock" ,python2-mock)    ; for tests
       ("python-pytz" ,python2-pytz)))  ; for tests
    (home-page "https://www.genes.mit.edu/burgelab/miso/index.html")
    (synopsis "Mixture of Isoforms model for RNA-Seq isoform quantitation")
    (description
     "MISO (Mixture-of-Isoforms) is a probabilistic framework that quantitates
the expression level of alternatively spliced genes from RNA-Seq data, and
identifies differentially regulated isoforms or exons across samples.  By
modeling the generative process by which reads are produced from isoforms in
RNA-Seq, the MISO model uses Bayesian inference to compute the probability
that a read originated from a particular isoform.")
    (license license:gpl2)))

(define-public muscle
  (package
    (name "muscle")
    (version "3.8.1551")
    (source (origin
              (method url-fetch/tarbomb)
              (uri (string-append
                    "http://www.drive5.com/muscle/muscle_src_"
                    version ".tar.gz"))
              (sha256
               (base32
                "0bj8kj7sdizy3987zx6w7axihk40fk8rn76mpbqqjcnd64i5a367"))))
    (build-system gnu-build-system)
    (arguments
     `(#:make-flags (list "LDLIBS = -lm")
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (replace 'check
           ;; There are no tests, so just test if it runs.
           (lambda _ (invoke "./muscle" "-version") #t))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin")))
               (install-file "muscle" bin)
               #t))))))
    (home-page "http://www.drive5.com/muscle")
    (synopsis "Multiple sequence alignment program")
    (description
     "MUSCLE aims to be a fast and accurate multiple sequence alignment
program for nucleotide and protein sequences.")
    ;; License information found in 'muscle -h' and usage.cpp.
    (license license:public-domain)))

(define-public newick-utils
  ;; There are no recent releases so we package from git.
  (let ((commit "da121155a977197cab9fbb15953ca1b40b11eb87"))
    (package
      (name "newick-utils")
      (version (string-append "1.6-1." (string-take commit 8)))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "https://github.com/tjunier/newick_utils")
                      (commit commit)))
                (file-name (string-append name "-" version "-checkout"))
                (sha256
                 (base32
                  "1hkw21rq1mwf7xp0rmbb2gqc0i6p11108m69i7mr7xcjl268pxnb"))))
    (build-system gnu-build-system)
    (inputs
     ;; XXX: TODO: Enable Lua and Guile bindings.
     ;; https://github.com/tjunier/newick_utils/issues/13
     `(("libxml2" ,libxml2)
       ("flex" ,flex)
       ("bison" ,bison)))
    (native-inputs
     `(("autoconf" ,autoconf)
       ("automake" ,automake)
       ("libtool" ,libtool)))
    (synopsis "Programs for working with newick format phylogenetic trees")
    (description
     "Newick-utils is a suite of utilities for processing phylogenetic trees
in Newick format.  Functions include re-rooting, extracting subtrees,
trimming, pruning, condensing, drawing (ASCII graphics or SVG).")
    (home-page "https://github.com/tjunier/newick_utils")
    (license license:bsd-3))))

(define-public orfm
  (package
    (name "orfm")
    (version "0.7.1")
    (source (origin
              (method url-fetch)
              (uri (string-append
                    "https://github.com/wwood/OrfM/releases/download/v"
                    version "/orfm-" version ".tar.gz"))
              (sha256
               (base32
                "16iigyr2gd8x0imzkk1dr3k5xsds9bpmwg31ayvjg0f4pir9rwqr"))))
    (build-system gnu-build-system)
    (inputs `(("zlib" ,zlib)))
    (native-inputs
     `(("ruby-bio-commandeer" ,ruby-bio-commandeer)
       ("ruby-rspec" ,ruby-rspec)
       ("ruby" ,ruby)))
    (synopsis "Simple and not slow open reading frame (ORF) caller")
    (description
     "An ORF caller finds stretches of DNA that, when translated, are not
interrupted by stop codons.  OrfM finds and prints these ORFs.")
    (home-page "https://github.com/wwood/OrfM")
    (license license:lgpl3+)))

(define-public python2-pbcore
  (package
    (name "python2-pbcore")
    (version "1.2.10")
    (source (origin
              (method url-fetch)
              (uri (pypi-uri "pbcore" version))
              (sha256
               (base32
                "1kjmv891d6qbpp4shhhvkl02ff4q5xlpnls2513sm2cjcrs52f1i"))))
    (build-system python-build-system)
    (arguments
     `(#:python ,python-2               ;pbcore < 2.0 requires Python 2.7
       #:phases (modify-phases %standard-phases
                  (add-after 'unpack 'remove-sphinx-dependency
                    (lambda _
                      ;; Sphinx is only required for documentation tests, which
                      ;; we do not run; furthermore it depends on python2-sphinx
                      ;; which is no longer maintained.
                      (substitute* "requirements-dev.txt"
                        (("^sphinx") ""))
                      #t)))))
    (propagated-inputs
     `(("python-cython" ,python2-cython)
       ("python-numpy" ,python2-numpy)
       ("python-pysam" ,python2-pysam)
       ("python-h5py" ,python2-h5py)))
    (native-inputs
     `(("python-nose" ,python2-nose)
       ("python-pyxb" ,python2-pyxb)))
    (home-page "https://pacificbiosciences.github.io/pbcore/")
    (synopsis "Library for reading and writing PacBio data files")
    (description
     "The pbcore package provides Python APIs for interacting with PacBio data
files and writing bioinformatics applications.")
    (license license:bsd-3)))

(define-public python2-warpedlmm
  (package
    (name "python2-warpedlmm")
    (version "0.21")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "WarpedLMM" version ".zip"))
       (sha256
        (base32
         "1agfz6zqa8nc6cw47yh0s3y14gkpa9wqazwcj7mwwj3ffnw39p3j"))))
    (build-system python-build-system)
    (arguments
     `(#:python ,python-2  ; requires Python 2.7
       #:tests? #f ; test data are not included
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'use-weave
           (lambda _
             (substitute* "warpedlmm/util/linalg.py"
               (("from scipy import linalg, weave")
                "from scipy import linalg\nimport weave"))
             #t)))))
    (propagated-inputs
     `(("python-scipy" ,python2-scipy)
       ("python-numpy" ,python2-numpy)
       ("python-matplotlib" ,python2-matplotlib)
       ("python-fastlmm" ,python2-fastlmm)
       ("python-pandas" ,python2-pandas)
       ("python-pysnptools" ,python2-pysnptools)
       ("python-weave" ,python2-weave)))
    (native-inputs
     `(("python-mock" ,python2-mock)
       ("python-nose" ,python2-nose)
       ("unzip" ,unzip)))
    (home-page "https://github.com/PMBio/warpedLMM")
    (synopsis "Implementation of warped linear mixed models")
    (description
     "WarpedLMM is a Python implementation of the warped linear mixed model,
which automatically learns an optimal warping function (or transformation) for
the phenotype as it models the data.")
    (license license:asl2.0)))

(define-public pbtranscript-tofu
  (let ((commit "8f5467fe6a4472bcfb4226c8720993c8507adfe4"))
    (package
      (name "pbtranscript-tofu")
      (version (string-append "2.2.3." (string-take commit 7)))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "https://github.com/PacificBiosciences/cDNA_primer")
                      (commit commit)))
                (file-name (string-append name "-" version "-checkout"))
                (sha256
                 (base32
                  "1lgnpi35ihay42qx0b6yl3kkgra723i413j33kvs0kvs61h82w0f"))
                (modules '((guix build utils)))
                (snippet
                 '(begin
                    ;; remove bundled Cython sources
                    (delete-file "pbtranscript-tofu/pbtranscript/Cython-0.20.1.tar.gz")
                    #t))))
      (build-system python-build-system)
      (arguments
       `(#:python ,python-2
         ;; FIXME: Tests fail with "No such file or directory:
         ;; pbtools/pbtranscript/modified_bx_intervals/intersection_unique.so"
         #:tests? #f
         #:phases
         (modify-phases %standard-phases
           (add-after 'unpack 'enter-directory
            (lambda _
              (chdir "pbtranscript-tofu/pbtranscript/")
              #t))
           ;; With setuptools version 18.0 and later this setup.py hack causes
           ;; a build error, so we disable it.
           (add-after 'enter-directory 'patch-setuppy
            (lambda _
              (substitute* "setup.py"
                (("if 'setuptools.extension' in sys.modules:")
                 "if False:"))
              #t)))))
      (inputs
       `(("python-numpy" ,python2-numpy)
         ("python-bx-python" ,python2-bx-python)
         ("python-networkx" ,python2-networkx)
         ("python-scipy" ,python2-scipy)
         ("python-pbcore" ,python2-pbcore)
         ("python-h5py" ,python2-h5py)))
      (native-inputs
       `(("python-cython" ,python2-cython)
         ("python-nose" ,python2-nose)))
      (home-page "https://github.com/PacificBiosciences/cDNA_primer")
      (synopsis "Analyze transcriptome data generated with the Iso-Seq protocol")
      (description
       "pbtranscript-tofu contains scripts to analyze transcriptome data
generated using the PacBio Iso-Seq protocol.")
      (license license:bsd-3))))

(define-public prank
  (package
    (name "prank")
    (version "170427")
    (source (origin
              (method url-fetch)
              (uri (string-append
                    "http://wasabiapp.org/download/prank/prank.source."
                    version ".tgz"))
              (sha256
               (base32
                "0nc8g9c5rkdxcir46s0in9ci1sxwzbjibxrvkksf22ybnplvagk2"))))
    (build-system gnu-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'enter-src-dir
            (lambda _
              (chdir "src")
              #t))
         (add-after 'unpack 'remove-m64-flag
           ;; Prank will build with the correct 'bit-ness' without this flag
           ;; and this allows building on 32-bit machines.
           (lambda _ (substitute* "src/Makefile"
                                  (("-m64") ""))
             #t))
         (delete 'configure)
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin"))
                    (man (string-append out "/share/man/man1"))
                    (path (string-append
                           (assoc-ref %build-inputs "mafft") "/bin:"
                           (assoc-ref %build-inputs "exonerate") "/bin:"
                           (assoc-ref %build-inputs "bppsuite") "/bin")))
               (install-file "prank" bin)
               (wrap-program (string-append bin "/prank")
                 `("PATH" ":" prefix (,path)))
               (install-file "prank.1" man))
             #t)))))
    (inputs
     `(("mafft" ,mafft)
       ("exonerate" ,exonerate)
       ("bppsuite" ,bppsuite)))
    (home-page "http://wasabiapp.org/software/prank/")
    (synopsis "Probabilistic multiple sequence alignment program")
    (description
     "PRANK is a probabilistic multiple sequence alignment program for DNA,
codon and amino-acid sequences.  It is based on a novel algorithm that treats
insertions correctly and avoids over-estimation of the number of deletion
events.  In addition, PRANK borrows ideas from maximum likelihood methods used
in phylogenetics and correctly takes into account the evolutionary distances
between sequences.  Lastly, PRANK allows for defining a potential structure
for sequences to be aligned and then, simultaneously with the alignment,
predicts the locations of structural units in the sequences.")
    (license license:gpl2+)))

(define-public proteinortho
  (package
    (name "proteinortho")
    (version "6.0.14")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://gitlab.com/paulklemm_PHD/proteinortho.git")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "0pmy617zy2z2w6hjqxjhf3rzikf5n3mpia80ysq8233vfr7wrzff"))
              (modules '((guix build utils)))
              (snippet
               '(begin
                  ;; remove pre-built scripts
                  (delete-file-recursively "src/BUILD/")
                  #t))))
    (build-system gnu-build-system)
    (arguments
     `(#:test-target "test"
       #:make-flags '("CC=gcc")
       #:phases
       (modify-phases %standard-phases
         (replace 'configure
           ;; There is no configure script, so we modify the Makefile directly.
           (lambda* (#:key outputs #:allow-other-keys)
             (substitute* "Makefile"
               (("INSTALLDIR=.*")
                (string-append
                 "INSTALLDIR=" (assoc-ref outputs "out") "/bin\n")))
             #t))
         (add-before 'install 'make-install-directory
           ;; The install directory is not created during 'make install'.
           (lambda* (#:key outputs #:allow-other-keys)
             (mkdir-p (string-append (assoc-ref outputs "out") "/bin"))
             #t))
         (add-after 'install 'wrap-programs
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let ((path (getenv "PATH"))
                   (out (assoc-ref outputs "out")))
               (for-each (lambda (script)
                           (wrap-script script `("PATH" ":" prefix (,path))))
                         (cons (string-append out "/bin/proteinortho")
                               (find-files out "\\.(pl|py)$"))))
             #t)))))
    (inputs
     `(("guile" ,guile-3.0) ; for wrap-script
       ("diamond" ,diamond)
       ("perl" ,perl)
       ("python" ,python-wrapper)
       ("blast+" ,blast+)
       ("lapack" ,lapack)
       ("openblas" ,openblas)))
    (native-inputs
     `(("which" ,which)))
    (home-page "http://www.bioinf.uni-leipzig.de/Software/proteinortho")
    (synopsis "Detect orthologous genes across species")
    (description
     "Proteinortho is a tool to detect orthologous genes across different
species.  For doing so, it compares similarities of given gene sequences and
clusters them to find significant groups.  The algorithm was designed to handle
large-scale data and can be applied to hundreds of species at once.")
    (license license:gpl3+)))

(define-public pyicoteo
  (package
    (name "pyicoteo")
    (version "2.0.7")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://bitbucket.org/regulatorygenomicsupf/pyicoteo.git")
             (commit (string-append "v" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "0hz5g8d25lbjy1wpscr490l0lmyvaix893hhax4fxnh1h9w34w8p"))))
    (build-system python-build-system)
    (arguments
     `(#:python ,python-2 ; does not work with Python 3
       #:tests? #f))      ; there are no tests
    (inputs
     `(("python2-matplotlib" ,python2-matplotlib)))
    (home-page "https://bitbucket.org/regulatorygenomicsupf/pyicoteo")
    (synopsis "Analyze high-throughput genetic sequencing data")
    (description
     "Pyicoteo is a suite of tools for the analysis of high-throughput genetic
sequencing data.  It works with genomic coordinates.  There are currently six
different command-line tools:

@enumerate
@item pyicoregion: for generating exploratory regions automatically;
@item pyicoenrich: for differential enrichment between two conditions;
@item pyicoclip: for calling CLIP-Seq peaks without a control;
@item pyicos: for genomic coordinates manipulation;
@item pyicoller: for peak calling on punctuated ChIP-Seq;
@item pyicount: to count how many reads from N experiment files overlap in a
  region file;
@item pyicotrocol: to combine operations from pyicoteo.
@end enumerate\n")
    (license license:gpl3+)))

(define-public prodigal
  (package
    (name "prodigal")
    ;; Check for a new home page when updating this package:
    ;; https://github.com/hyattpd/Prodigal/issues/36#issuecomment-536617588
    (version "2.6.3")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/hyattpd/Prodigal")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1fs1hqk83qjbjhrvhw6ni75zakx5ki1ayy3v6wwkn3xvahc9hi5s"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f                      ; no check target
       #:make-flags (list (string-append "INSTALLDIR="
                                         (assoc-ref %outputs "out")
                                         "/bin"))
       #:phases
       (modify-phases %standard-phases
         (delete 'configure))))
    (home-page "https://github.com/hyattpd/Prodigal")
    (synopsis "Protein-coding gene prediction for Archaea and Bacteria")
    (description
     "Prodigal runs smoothly on finished genomes, draft genomes, and
metagenomes, providing gene predictions in GFF3, Genbank, or Sequin table
format.  It runs quickly, in an unsupervised fashion, handles gaps, handles
partial genes, and identifies translation initiation sites.")
    (license license:gpl3+)))

(define-public roary
  (package
    (name "roary")
    (version "3.12.0")
    (source
     (origin
       (method url-fetch)
       (uri (string-append
             "mirror://cpan/authors/id/A/AJ/AJPAGE/Bio-Roary-"
             version ".tar.gz"))
       (sha256
        (base32
         "0qxrds9wx7cfhlkihrp6697kx0flhhxymap9fwan0b3rbdhcnmff"))))
    (build-system perl-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (delete 'build)
         (replace 'check
           (lambda _
             ;; The tests are not run by default, so we run each test file
             ;; directly.
             (setenv "PATH" (string-append (getcwd) "/bin" ":"
                                           (getenv "PATH")))
             (setenv "PERL5LIB" (string-append (getcwd) "/lib" ":"
                                               (getenv "PERL5LIB")))
             (for-each (lambda (file)
                         (display file)(display "\n")
                         (invoke "perl" file))
                       (find-files "t" ".*\\.t$"))
             #t))
         (replace 'install
           ;; There is no 'install' target in the Makefile.
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin"))
                    (perl (string-append out "/lib/perl5/site_perl"))
                    (roary-plots "contrib/roary_plots"))
               (mkdir-p bin)
               (mkdir-p perl)
               (copy-recursively "bin" bin)
               (copy-recursively "lib" perl)
               #t)))
         (add-after 'install 'wrap-programs
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (perl5lib (getenv "PERL5LIB"))
                    (path (getenv "PATH")))
               (for-each (lambda (prog)
                           (let ((binary (string-append out "/" prog)))
                             (wrap-program binary
                               `("PERL5LIB" ":" prefix
                                 (,(string-append perl5lib ":" out
                                                  "/lib/perl5/site_perl"))))
                             (wrap-program binary
                               `("PATH" ":" prefix
                                 (,(string-append path ":" out "/bin"))))))
                         (find-files "bin" ".*[^R]$"))
               (let ((file
                      (string-append out "/bin/roary-create_pan_genome_plots.R"))
                     (r-site-lib (getenv "R_LIBS_SITE"))
                     (coreutils-path
                      (string-append (assoc-ref inputs "coreutils") "/bin")))
                 (wrap-program file
                   `("R_LIBS_SITE" ":" prefix
                     (,(string-append r-site-lib ":" out "/site-library/"))))
                 (wrap-program file
                   `("PATH" ":" prefix
                     (,(string-append coreutils-path ":" out "/bin"))))))
             #t)))))
    (native-inputs
     `(("perl-env-path" ,perl-env-path)
       ("perl-test-files" ,perl-test-files)
       ("perl-test-most" ,perl-test-most)
       ("perl-test-output" ,perl-test-output)))
    (inputs
     `(("perl-array-utils" ,perl-array-utils)
       ("bioperl" ,bioperl-minimal)
       ("perl-digest-md5-file" ,perl-digest-md5-file)
       ("perl-exception-class" ,perl-exception-class)
       ("perl-file-find-rule" ,perl-file-find-rule)
       ("perl-file-grep" ,perl-file-grep)
       ("perl-file-slurper" ,perl-file-slurper)
       ("perl-file-which" ,perl-file-which)
       ("perl-graph" ,perl-graph)
       ("perl-graph-readwrite" ,perl-graph-readwrite)
       ("perl-log-log4perl" ,perl-log-log4perl)
       ("perl-moose" ,perl-moose)
       ("perl-perlio-utf8_strict" ,perl-perlio-utf8_strict)
       ("perl-text-csv" ,perl-text-csv)
       ("bedtools" ,bedtools)
       ("cd-hit" ,cd-hit)
       ("blast+" ,blast+)
       ("mcl" ,mcl)
       ("parallel" ,parallel)
       ("prank" ,prank)
       ("mafft" ,mafft)
       ("fasttree" ,fasttree)
       ("grep" ,grep)
       ("sed" ,sed)
       ("gawk" ,gawk)
       ("r-minimal" ,r-minimal)
       ("r-ggplot2" ,r-ggplot2)
       ("coreutils" ,coreutils)))
    (home-page "https://sanger-pathogens.github.io/Roary/")
    (synopsis "High speed stand-alone pan genome pipeline")
    (description
     "Roary is a high speed stand alone pan genome pipeline, which takes
annotated assemblies in GFF3 format (produced by the Prokka program) and
calculates the pan genome.  Using a standard desktop PC, it can analyse
datasets with thousands of samples, without compromising the quality of the
results.  128 samples can be analysed in under 1 hour using 1 GB of RAM and a
single processor.  Roary is not intended for metagenomics or for comparing
extremely diverse sets of genomes.")
    (license license:gpl3)))

(define-public raxml
  (package
    (name "raxml")
    (version "8.2.12")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/stamatak/standard-RAxML")
             (commit (string-append "v" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "1jqjzhch0rips0vp04prvb8vmc20c5pdmsqn8knadcf91yy859fh"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; There are no tests.
       ;; Use 'standard' Makefile rather than SSE or AVX ones.
       #:make-flags (list "-f" "Makefile.HYBRID.gcc")
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin"))
                    (executable "raxmlHPC-HYBRID"))
               (install-file executable bin)
               (symlink (string-append bin "/" executable) "raxml"))
             #t)))))
    (inputs
     `(("openmpi" ,openmpi)))
    (home-page "https://cme.h-its.org/exelixis/web/software/raxml/index.html")
    (synopsis "Randomized Axelerated Maximum Likelihood phylogenetic trees")
    (description
     "RAxML is a tool for phylogenetic analysis and post-analysis of large
phylogenies.")
    ;; The source includes x86 specific code
    (supported-systems '("x86_64-linux" "i686-linux"))
    (license license:gpl2+)))

(define-public rsem
  (package
    (name "rsem")
    (version "1.3.1")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/deweylab/RSEM")
             (commit (string-append "v" version))))
       (sha256
        (base32 "1jlq11d1p8qp64w75yj8cnbbd1a93viq10pzsbwal7vdn8fg13j1"))
       (file-name (git-file-name name version))
       (modules '((guix build utils)))
       (snippet
        '(begin
           ;; remove bundled copy of boost and samtools
           (delete-file-recursively "boost")
           (delete-file-recursively "samtools-1.3")
           #t))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ;no "check" target
       #:make-flags
       (list (string-append "BOOST="
                            (assoc-ref %build-inputs "boost")
                            "/include/")
             (string-append "SAMHEADERS="
                            (assoc-ref %build-inputs "htslib")
                            "/include/htslib/sam.h")
             (string-append "SAMLIBS="
                            (assoc-ref %build-inputs "htslib")
                            "/lib/libhts.a"))
       #:phases
       (modify-phases %standard-phases
         ;; No "configure" script.
         ;; Do not build bundled samtools library.
         (replace 'configure
           (lambda _
             (substitute* "Makefile"
               (("^all : \\$\\(PROGRAMS\\).*") "all: $(PROGRAMS)\n")
               (("^\\$\\(SAMLIBS\\).*") ""))
             #t))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (string-append (assoc-ref outputs "out")))
                    (bin (string-append out "/bin/"))
                    (perl (string-append out "/lib/perl5/site_perl")))
               (mkdir-p bin)
               (mkdir-p perl)
               (for-each (lambda (file)
                           (install-file file bin))
                         (find-files "." "rsem-.*"))
               (install-file "rsem_perl_utils.pm" perl))
             #t))
         (add-after 'install 'wrap-program
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((out (assoc-ref outputs "out")))
               (for-each (lambda (prog)
                           (wrap-program (string-append out "/bin/" prog)
                             `("PERL5LIB" ":" prefix
                               (,(string-append out "/lib/perl5/site_perl")))))
                         '("rsem-calculate-expression"
                           "rsem-control-fdr"
                           "rsem-generate-data-matrix"
                           "rsem-generate-ngvector"
                           "rsem-plot-transcript-wiggles"
                           "rsem-prepare-reference"
                           "rsem-run-ebseq"
                           "rsem-run-prsem-testing-procedure")))
             #t)))))
    (inputs
     `(("boost" ,boost)
       ("r-minimal" ,r-minimal)
       ("perl" ,perl)
       ("htslib" ,htslib-1.3)
       ("zlib" ,zlib)))
    (home-page "http://deweylab.biostat.wisc.edu/rsem/")
    (synopsis "Estimate gene expression levels from RNA-Seq data")
    (description
     "RSEM is a software package for estimating gene and isoform expression
levels from RNA-Seq data.  The RSEM package provides a user-friendly
interface, supports threads for parallel computation of the EM algorithm,
single-end and paired-end read data, quality scores, variable-length reads and
RSPD estimation.  In addition, it provides posterior mean and 95% credibility
interval estimates for expression levels.  For visualization, it can generate
BAM and Wiggle files in both transcript-coordinate and genomic-coordinate.")
    (license license:gpl3+)))

(define-public rseqc
  (package
    (name "rseqc")
    (version "3.0.1")
    (source
     (origin
       (method url-fetch)
       (uri
        (string-append "mirror://sourceforge/rseqc/"
                       "RSeQC-" version ".tar.gz"))
       (sha256
        (base32
         "0gbb9iyb7swiv5455fm5rg98r7l6qn27v564yllqjd574hncpx6m"))))
    (build-system python-build-system)
    (inputs
     `(("python-cython" ,python-cython)
       ("python-bx-python" ,python-bx-python)
       ("python-pybigwig" ,python-pybigwig)
       ("python-pysam" ,python-pysam)
       ("python-numpy" ,python-numpy)
       ("zlib" ,zlib)))
    (native-inputs
     `(("python-nose" ,python-nose)))
    (home-page "http://rseqc.sourceforge.net/")
    (synopsis "RNA-seq quality control package")
    (description
     "RSeQC provides a number of modules that can comprehensively evaluate
high throughput sequence data, especially RNA-seq data.  Some basic modules
inspect sequence quality, nucleotide composition bias, PCR bias and GC bias,
while RNA-seq specific modules evaluate sequencing saturation, mapped reads
distribution, coverage uniformity, strand specificity, etc.")
    (license license:gpl3+)))

(define-public seek
  ;; There are no release tarballs.  According to the installation
  ;; instructions at http://seek.princeton.edu/installation.jsp, the latest
  ;; stable release is identified by this changeset ID.
  (let ((changeset "2329130")
        (revision "1"))
    (package
      (name "seek")
      (version (string-append "0-" revision "." changeset))
      (source (origin
                (method hg-fetch)
                (uri (hg-reference
                      (url "https://bitbucket.org/libsleipnir/sleipnir")
                      (changeset changeset)))
                (file-name (string-append name "-" version "-checkout"))
                (sha256
                 (base32
                  "0qrvilwh18dpbhkf92qvxbmay0j75ra3jg2wrhz67gf538zzphsx"))))
      (build-system gnu-build-system)
      (arguments
       `(#:modules ((srfi srfi-1)
                    (guix build gnu-build-system)
                    (guix build utils))
         #:phases
         (let ((dirs '("SeekMiner"
                       "SeekEvaluator"
                       "SeekPrep"
                       "Distancer"
                       "Data2DB"
                       "PCL2Bin")))
           (modify-phases %standard-phases
             (replace 'bootstrap
               (lambda _
                 (substitute* "gen_tools_am"
                   (("/usr/bin/env.*") (which "perl")))
                 (invoke "bash" "gen_auto")
                 #t))
             (add-after 'build 'build-additional-tools
               (lambda* (#:key make-flags #:allow-other-keys)
                 (for-each (lambda (dir)
                             (with-directory-excursion (string-append "tools/" dir)
                               (apply invoke "make" make-flags)))
                           dirs)
                 #t))
             (add-after 'install 'install-additional-tools
               (lambda* (#:key make-flags #:allow-other-keys)
                 (for-each (lambda (dir)
                             (with-directory-excursion (string-append "tools/" dir)
                               (apply invoke `("make" ,@make-flags "install"))))
                           dirs)
                 #t))))))
      (inputs
       `(("gsl" ,gsl)
         ("boost" ,boost)
         ("libsvm" ,libsvm)
         ("readline" ,readline)
         ("gengetopt" ,gengetopt)
         ("log4cpp" ,log4cpp)))
      (native-inputs
       `(("autoconf" ,autoconf)
         ("automake" ,automake)
         ("perl" ,perl)))
      (home-page "http://seek.princeton.edu")
      (synopsis "Gene co-expression search engine")
      (description
       "SEEK is a computational gene co-expression search engine.  SEEK provides
biologists with a way to navigate the massive human expression compendium that
now contains thousands of expression datasets.  SEEK returns a robust ranking
of co-expressed genes in the biological area of interest defined by the user's
query genes.  It also prioritizes thousands of expression datasets according
to the user's query of interest.")
      (license license:cc-by3.0))))

(define-public samtools
  (package
    (name "samtools")
    (version "1.11")
    (source
     (origin
       (method url-fetch)
       (uri
        (string-append "mirror://sourceforge/samtools/samtools/"
                       version "/samtools-" version ".tar.bz2"))
       (sha256
        (base32
         "1dp5wknak4arnw5ghhif9mmljlfnw5bgm91wib7z0j8wdjywx0z2"))
       (modules '((guix build utils)))
       (snippet '(begin
                   ;; Delete bundled htslib.
                   (delete-file-recursively "htslib-1.11")
                   #t))))
    (build-system gnu-build-system)
    (arguments
     `(#:modules ((ice-9 ftw)
                  (ice-9 regex)
                  (guix build gnu-build-system)
                  (guix build utils))
       #:configure-flags (list "--with-ncurses")
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'patch-tests
           (lambda _
             (substitute* "test/test.pl"
               ;; The test script calls out to /bin/bash
               (("/bin/bash") (which "bash")))
             #t))
         (add-after 'install 'install-library
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((lib (string-append (assoc-ref outputs "out") "/lib")))
               (install-file "libbam.a" lib)
               #t)))
         (add-after 'install 'install-headers
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((include (string-append (assoc-ref outputs "out")
                                           "/include/samtools/")))
               (for-each (lambda (file)
                           (install-file file include))
                         (scandir "." (lambda (name) (string-match "\\.h$" name))))
               #t))))))
    (native-inputs `(("pkg-config" ,pkg-config)))
    (inputs
     `(("htslib" ,htslib)
       ("ncurses" ,ncurses)
       ("perl" ,perl)
       ("python" ,python)
       ("zlib" ,zlib)))
    (home-page "http://samtools.sourceforge.net")
    (synopsis "Utilities to efficiently manipulate nucleotide sequence alignments")
    (description
     "Samtools implements various utilities for post-processing nucleotide
sequence alignments in the SAM, BAM, and CRAM formats, including indexing,
variant calling (in conjunction with bcftools), and a simple alignment
viewer.")
    (license license:expat)))

(define-public samtools-1.9
  (package (inherit samtools)
    (name "samtools")
    (version "1.9")
    (source
     (origin
       (method url-fetch)
       (uri
        (string-append "mirror://sourceforge/samtools/samtools/"
                       version "/samtools-" version ".tar.bz2"))
       (sha256
        (base32
         "10ilqbmm7ri8z431sn90lvbjwizd0hhkf9rcqw8j823hf26nhgq8"))
       (modules '((guix build utils)))
       (snippet '(begin
                   ;; Delete bundled htslib.
                   (delete-file-recursively "htslib-1.9")
                   #t))))
    (inputs
     `(("htslib" ,htslib-1.9)
       ("ncurses" ,ncurses)
       ("perl" ,perl)
       ("python" ,python)
       ("zlib" ,zlib)))))

(define-public samtools-0.1
  ;; This is the most recent version of the 0.1 line of samtools.  The input
  ;; and output formats differ greatly from that used and produced by samtools
  ;; 1.x and is still used in many bioinformatics pipelines.
  (package (inherit samtools)
    (version "0.1.19")
    (source
     (origin
       (method url-fetch)
       (uri
        (string-append "mirror://sourceforge/samtools/samtools/"
                       version "/samtools-" version ".tar.bz2"))
       (sha256
        (base32 "1m33xsfwz0s8qi45lylagfllqg7fphf4dr0780rsvw75av9wk06h"))))
    (arguments
     `(#:tests? #f ;no "check" target
       #:make-flags
       (list "LIBCURSES=-lncurses")
       ,@(substitute-keyword-arguments (package-arguments samtools)
           ((#:phases phases)
            `(modify-phases ,phases
               (replace 'install
                 (lambda* (#:key outputs #:allow-other-keys)
                   (let ((bin (string-append
                               (assoc-ref outputs "out") "/bin")))
                     (mkdir-p bin)
                     (install-file "samtools" bin)
                     #t)))
               (delete 'patch-tests)
               (delete 'configure))))))))

(define-public mosaik
  (let ((commit "5c25216d3522d6a33e53875cd76a6d65001e4e67"))
    (package
      (name "mosaik")
      (version "2.2.30")
      (source (origin
                ;; There are no release tarballs nor tags.
                (method git-fetch)
                (uri (git-reference
                      (url "https://github.com/wanpinglee/MOSAIK")
                      (commit commit)))
                (file-name (string-append name "-" version))
                (sha256
                 (base32
                  "17gj3s07cm77r41z92awh0bim7w7q7fbn0sf5nkqmcm1vw052qgw"))))
      (build-system gnu-build-system)
      (arguments
       `(#:tests? #f ; no tests
         #:make-flags (list "CC=gcc")
         #:phases
         (modify-phases %standard-phases
           (replace 'configure
                    (lambda _ (chdir "src") #t))
           (replace 'install
                    (lambda* (#:key outputs #:allow-other-keys)
                      (let ((bin (string-append (assoc-ref outputs "out")
                                                "/bin")))
                        (mkdir-p bin)
                        (copy-recursively "../bin" bin)
                        #t))))))
      (inputs
       `(("perl" ,perl)
         ("zlib:static" ,zlib "static")
         ("zlib" ,zlib)))
      (supported-systems '("x86_64-linux"))
      (home-page "https://github.com/wanpinglee/MOSAIK")
      (synopsis "Map nucleotide sequence reads to reference genomes")
      (description
       "MOSAIK is a program for mapping second and third-generation sequencing
reads to a reference genome.  MOSAIK can align reads generated by all the
major sequencing technologies, including Illumina, Applied Biosystems SOLiD,
Roche 454, Ion Torrent and Pacific BioSciences SMRT.")
      ;; MOSAIK is released under the GPLv2+ with the exception of third-party
      ;; code released into the public domain:
      ;; 1. fastlz by Ariya Hidayat - http://www.fastlz.org/
      ;; 2. MD5 implementation - RSA Data Security, RFC 1321
      (license (list license:gpl2+ license:public-domain)))))

(define-public ngs-sdk
  (package
    (name "ngs-sdk")
    (version "2.10.5")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/ncbi/ngs")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1ix51c25hjn57w93qmwzw80xh2i34wx8j2hn7szh8p6w8i3az5qa"))))
    (build-system gnu-build-system)
    (arguments
     `(#:parallel-build? #f ; not supported
       #:tests? #f ; no "check" target
       #:phases
       (modify-phases %standard-phases
         (replace 'configure
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((out (assoc-ref outputs "out")))
               ;; Allow 'konfigure.perl' to find 'package.prl'.
               (setenv "PERL5LIB"
                       (string-append ".:" (getenv "PERL5LIB")))

               ;; The 'configure' script doesn't recognize things like
               ;; '--enable-fast-install'.
               (invoke "./configure"
                       (string-append "--build-prefix=" (getcwd) "/build")
                       (string-append "--prefix=" out))
               #t)))
         (add-after 'unpack 'enter-dir
           (lambda _ (chdir "ngs-sdk") #t)))))
    (native-inputs `(("perl" ,perl)))
    ;; According to the test
    ;;   unless ($MARCH =~ /x86_64/i || $MARCH =~ /i?86/i)
    ;; in ngs-sdk/setup/konfigure.perl
    (supported-systems '("i686-linux" "x86_64-linux"))
    (home-page "https://github.com/ncbi/ngs")
    (synopsis "API for accessing Next Generation Sequencing data")
    (description
     "NGS is a domain-specific API for accessing reads, alignments and pileups
produced from Next Generation Sequencing.  The API itself is independent from
any particular back-end implementation, and supports use of multiple back-ends
simultaneously.")
    (license license:public-domain)))

(define-public java-ngs
  (package (inherit ngs-sdk)
    (name "java-ngs")
    (arguments
     `(,@(substitute-keyword-arguments
             `(#:modules ((guix build gnu-build-system)
                          (guix build utils)
                          (srfi srfi-1)
                          (srfi srfi-26))
                         ,@(package-arguments ngs-sdk))
           ((#:phases phases)
            `(modify-phases ,phases
               (replace 'enter-dir (lambda _ (chdir "ngs-java") #t)))))))
    (inputs
     `(("jdk" ,icedtea "jdk")
       ("ngs-sdk" ,ngs-sdk)))
    (synopsis "Java bindings for NGS SDK")))

(define-public ncbi-vdb
  (package
    (name "ncbi-vdb")
    (version "2.10.6")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/ncbi/ncbi-vdb")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "0m8hlxscidsfqm9x9fyi62q6lpf1dv5115kgjjgnrkl49q9c27m6"))))
    (build-system gnu-build-system)
    (arguments
     `(#:parallel-build? #f ; not supported
       #:tests? #f ; no "check" target
       #:make-flags '("HAVE_HDF5=1")
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'make-files-writable
           (lambda _ (for-each make-file-writable (find-files "." ".*")) #t))
         (add-before 'configure 'set-perl-search-path
           (lambda _
             ;; Work around "dotless @INC" build failure.
             (setenv "PERL5LIB"
                     (string-append (getcwd) "/setup:"
                                    (getenv "PERL5LIB")))
             #t))
         ;; See https://github.com/ncbi/ncbi-vdb/issues/14
         (add-after 'unpack 'patch-krypto-flags
           (lambda _
             (substitute* "libs/krypto/Makefile"
               (("-Wa,-march=generic64\\+aes") "")
               (("-Wa,-march=generic64\\+sse4") ""))
             #t))
         (replace 'configure
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let ((out (assoc-ref outputs "out")))
               ;; Override include path for libmagic
               (substitute* "setup/package.prl"
                 (("name => 'magic', Include => '/usr/include'")
                  (string-append "name=> 'magic', Include => '"
                                 (assoc-ref inputs "libmagic")
                                 "/include" "'")))

               ;; Install kdf5 library (needed by sra-tools)
               (substitute* "build/Makefile.install"
                 (("LIBRARIES_TO_INSTALL =")
                  "LIBRARIES_TO_INSTALL = kdf5.$(VERSION_LIBX) kdf5.$(VERSION_SHLX)"))

               (substitute* "build/Makefile.env"
                 (("CFLAGS	=" prefix)
                  (string-append prefix "-msse2 ")))

               ;; Override search path for ngs-java
               (substitute* "setup/package.prl"
                 (("/usr/local/ngs/ngs-java")
                  (assoc-ref inputs "java-ngs")))

               ;; The 'configure' script doesn't recognize things like
               ;; '--enable-fast-install'.
               (invoke "./configure"
                       (string-append "--build-prefix=" (getcwd) "/build")
                       (string-append "--prefix=" (assoc-ref outputs "out"))
                       (string-append "--debug")
                       (string-append "--with-xml2-prefix="
                                      (assoc-ref inputs "libxml2"))
                       (string-append "--with-ngs-sdk-prefix="
                                      (assoc-ref inputs "ngs-sdk"))
                       (string-append "--with-hdf5-prefix="
                                      (assoc-ref inputs "hdf5")))
               #t)))
         (add-after 'install 'install-interfaces
           (lambda* (#:key outputs #:allow-other-keys)
             ;; Install interface libraries.  On i686 the interface libraries
             ;; are installed to "linux/gcc/i386", so we need to use the Linux
             ;; architecture name ("i386") instead of the target system prefix
             ;; ("i686").
             (mkdir (string-append (assoc-ref outputs "out") "/ilib"))
             (copy-recursively (string-append "build/ncbi-vdb/linux/gcc/"
                                              ,(system->linux-architecture
                                                (or (%current-target-system)
                                                    (%current-system)))
                                              "/rel/ilib")
                               (string-append (assoc-ref outputs "out")
                                              "/ilib"))
             ;; Install interface headers
             (copy-recursively "interfaces"
                               (string-append (assoc-ref outputs "out")
                                              "/include"))
             #t))
         ;; These files are needed by sra-tools.
         (add-after 'install 'install-configuration-files
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((target (string-append (assoc-ref outputs "out") "/kfg")))
               (mkdir target)
               (install-file "libs/kfg/default.kfg" target)
               (install-file "libs/kfg/certs.kfg" target))
             #t)))))
    (inputs
     `(("libxml2" ,libxml2)
       ("ngs-sdk" ,ngs-sdk)
       ("java-ngs" ,java-ngs)
       ("libmagic" ,file)
       ("hdf5" ,hdf5)))
    (native-inputs `(("perl" ,perl)))
    ;; NCBI-VDB requires SSE capability.
    (supported-systems '("i686-linux" "x86_64-linux"))
    (home-page "https://github.com/ncbi/ncbi-vdb")
    (synopsis "Database engine for genetic information")
    (description
     "The NCBI-VDB library implements a highly compressed columnar data
warehousing engine that is most often used to store genetic information.
Databases are stored in a portable image within the file system, and can be
accessed/downloaded on demand across HTTP.")
    (license license:public-domain)))

(define-public plink
  (package
    (name "plink")
    (version "1.07")
    (source
     (origin
       (method url-fetch)
       (uri (string-append
             "http://pngu.mgh.harvard.edu/~purcell/plink/dist/plink-"
             version "-src.zip"))
       (sha256
        (base32 "0as8gxm4pjyc8dxmm1sl873rrd7wn5qs0l29nqfnl31x8i467xaa"))
       (patches (search-patches "plink-1.07-unclobber-i.patch"
                                "plink-endian-detection.patch"))))
    (build-system gnu-build-system)
    (arguments
     '(#:tests? #f ;no "check" target
       #:make-flags (list (string-append "LIB_LAPACK="
                                         (assoc-ref %build-inputs "lapack")
                                         "/lib/liblapack.so")
                          "WITH_LAPACK=1"
                          "FORCE_DYNAMIC=1"
                          ;; disable phoning home
                          "WITH_WEBCHECK=")
       #:phases
       (modify-phases %standard-phases
         ;; no "configure" script
         (delete 'configure)
         (replace 'install
                  (lambda* (#:key outputs #:allow-other-keys)
                    (let ((bin (string-append (assoc-ref outputs "out")
                                              "/bin/")))
                      (install-file "plink" bin)
                      #t))))))
    (inputs
     `(("zlib" ,zlib)
       ("lapack" ,lapack)))
    (native-inputs
     `(("unzip" ,unzip)))
    (home-page "http://pngu.mgh.harvard.edu/~purcell/plink/")
    (synopsis "Whole genome association analysis toolset")
    (description
     "PLINK is a whole genome association analysis toolset, designed to
perform a range of basic, large-scale analyses in a computationally efficient
manner.  The focus of PLINK is purely on analysis of genotype/phenotype data,
so there is no support for steps prior to this (e.g. study design and
planning, generating genotype or CNV calls from raw data).  Through
integration with gPLINK and Haploview, there is some support for the
subsequent visualization, annotation and storage of results.")
    ;; Code is released under GPLv2, except for fisher.h, which is under
    ;; LGPLv2.1+
    (license (list license:gpl2 license:lgpl2.1+))))

(define-public plink-ng
  (package (inherit plink)
    (name "plink-ng")
    (version "1.90b4")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/chrchang/plink-ng")
             (commit (string-append "v" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32 "02npdwgkpfkdnhw819rhj5kw02a5k5m90b14zq9zzya4hyg929c0"))))
    (build-system gnu-build-system)
    (arguments
     '(#:tests? #f ;no "check" target
       #:make-flags (list "BLASFLAGS=-llapack -lopenblas"
                          "CFLAGS=-Wall -O2 -DDYNAMIC_ZLIB=1"
                          "ZLIB=-lz"
                          "-f" "Makefile.std")
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'chdir
           (lambda _ (chdir "1.9") #t))
         (delete 'configure) ; no "configure" script
         (replace 'install
                  (lambda* (#:key outputs #:allow-other-keys)
                    (let ((bin (string-append (assoc-ref outputs "out")
                                              "/bin/")))
                      (install-file "plink" bin)
                      #t))))))
    (inputs
     `(("zlib" ,zlib)
       ("lapack" ,lapack)
       ("openblas" ,openblas)))
    (home-page "https://www.cog-genomics.org/plink/")
    (license license:gpl3+)))

(define-public smithlab-cpp
  (let ((revision "1")
        (commit "728a097bec88c6f4b8528b685932049e660eff2e"))
    (package
      (name "smithlab-cpp")
      (version (string-append "0." revision "." (string-take commit 7)))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "https://github.com/smithlabcode/smithlab_cpp")
                      (commit commit)))
                (file-name (string-append name "-" version "-checkout"))
                (sha256
                 (base32
                  "0d476lmj312xk77kr9fzrv7z1bv96yfyx0w7y62ycmnfbx32ll74"))))
      (build-system gnu-build-system)
      (arguments
       `(#:modules ((guix build gnu-build-system)
                    (guix build utils)
                    (srfi srfi-26))
         #:tests? #f ;no "check" target
         #:phases
         (modify-phases %standard-phases
           (add-after 'unpack 'use-samtools-headers
            (lambda _
              (substitute* '("SAM.cpp"
                             "SAM.hpp")
                (("sam.h") "samtools/sam.h"))
              #t))
           (replace 'install
            (lambda* (#:key outputs #:allow-other-keys)
              (let* ((out     (assoc-ref outputs "out"))
                     (lib     (string-append out "/lib"))
                     (include (string-append out "/include/smithlab-cpp")))
                (mkdir-p lib)
                (mkdir-p include)
                (for-each (cut install-file <> lib)
                          (find-files "." "\\.o$"))
                (for-each (cut install-file <> include)
                          (find-files "." "\\.hpp$")))
              #t))
           (delete 'configure))))
      (inputs
       `(("samtools" ,samtools-0.1)
         ("zlib" ,zlib)))
      (home-page "https://github.com/smithlabcode/smithlab_cpp")
      (synopsis "C++ helper library for functions used in Smith lab projects")
      (description
       "Smithlab CPP is a C++ library that includes functions used in many of
the Smith lab bioinformatics projects, such as a wrapper around Samtools data
structures, classes for genomic regions, mapped sequencing reads, etc.")
      (license license:gpl3+))))

(define-public preseq
  (package
    (name "preseq")
    (version "2.0.3")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://github.com/smithlabcode/preseq/"
                                  "releases/download/v" version
                                  "/preseq_v" version ".tar.bz2"))
              (sha256
               (base32 "149x9xmk1wy1gff85325yfzqc0qk4sgp1w6gbyj9cnji4x1dszbl"))
              (modules '((guix build utils)))
              (snippet '(begin
                          ;; Remove bundled samtools.
                          (delete-file-recursively "samtools")
                          #t))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ;no "check" target
       #:phases
       (modify-phases %standard-phases
         (delete 'configure))
       #:make-flags
       (list (string-append "PREFIX="
                            (assoc-ref %outputs "out"))
             (string-append "LIBBAM="
                            (assoc-ref %build-inputs "samtools")
                            "/lib/libbam.a")
             (string-append "SMITHLAB_CPP="
                            (assoc-ref %build-inputs "smithlab-cpp")
                            "/lib")
             "PROGS=preseq"
             "INCLUDEDIRS=$(SMITHLAB_CPP)/../include/smithlab-cpp $(SAMTOOLS_DIR)")))
    (inputs
     `(("gsl" ,gsl)
       ("samtools" ,samtools-0.1)
       ("smithlab-cpp" ,smithlab-cpp)
       ("zlib" ,zlib)))
    (home-page "http://smithlabresearch.org/software/preseq/")
    (synopsis "Program for analyzing library complexity")
    (description
     "The preseq package is aimed at predicting and estimating the complexity
of a genomic sequencing library, equivalent to predicting and estimating the
number of redundant reads from a given sequencing depth and how many will be
expected from additional sequencing using an initial sequencing experiment.
The estimates can then be used to examine the utility of further sequencing,
optimize the sequencing depth, or to screen multiple libraries to avoid low
complexity samples.")
    (license license:gpl3+)))

(define-public python-screed
  (package
    (name "python-screed")
    (version "1.0")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "screed" version))
       (sha256
        (base32
         "148vcb7w2wr6a4w6vs2bsxanbqibxfk490zbcbg4m61s8669zdjx"))))
    (build-system python-build-system)
    (arguments
     '(#:phases
       (modify-phases %standard-phases
         ;; Tests must be run after installation, as the "screed" command does
         ;; not exist right after building.
         (delete 'check)
         (add-after 'install 'check
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let ((out (assoc-ref outputs "out")))
               (setenv "PYTHONPATH"
                       (string-append out "/lib/python"
                                      (string-take (string-take-right
                                                    (assoc-ref inputs "python")
                                                    5) 3)
                                      "/site-packages:"
                                      (getenv "PYTHONPATH")))
               (setenv "PATH" (string-append out "/bin:" (getenv "PATH"))))
             (invoke "python" "setup.py" "test")
             #t)))))
    (native-inputs
     `(("python-pytest" ,python-pytest)
       ("python-pytest-cov" ,python-pytest-cov)
       ("python-pytest-runner" ,python-pytest-runner)))
    (inputs
     `(("python-bz2file" ,python-bz2file)))
    (home-page "https://github.com/dib-lab/screed/")
    (synopsis "Short read sequence database utilities")
    (description "Screed parses FASTA and FASTQ files and generates databases.
Values such as sequence name, sequence description, sequence quality and the
sequence itself can be retrieved from these databases.")
    (license license:bsd-3)))

(define-public python2-screed
  (package-with-python2 python-screed))

(define-public sra-tools
  (package
    (name "sra-tools")
    (version "2.10.6")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/ncbi/sra-tools")
             (commit version)))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "1cr2mijkfs5sm35ffjs6861qsd1qkgnhnbavdv65zg5d655abbjf"))))
    (build-system gnu-build-system)
    (arguments
     `(#:parallel-build? #f             ; not supported
       #:tests? #f                      ; no "check" target
       #:make-flags
       (list (string-append "DEFAULT_CRT="
                            (assoc-ref %build-inputs "ncbi-vdb")
                            "/kfg/certs.kfg")
             (string-append "DEFAULT_KFG="
                            (assoc-ref %build-inputs "ncbi-vdb")
                            "/kfg/default.kfg")
             (string-append "VDB_LIBDIR="
                            (assoc-ref %build-inputs "ncbi-vdb")
                            ,(if (string-prefix? "x86_64"
                                                 (or (%current-target-system)
                                                     (%current-system)))
                                 "/lib64"
                                 "/lib32")))
       #:phases
       (modify-phases %standard-phases
         (add-before 'configure 'set-perl-search-path
           (lambda _
             ;; Work around "dotless @INC" build failure.
             (setenv "PERL5LIB"
                     (string-append (getcwd) "/setup:"
                                    (getenv "PERL5LIB")))
             #t))
         (replace 'configure
           (lambda* (#:key inputs outputs #:allow-other-keys)
             ;; The build system expects a directory containing the sources and
             ;; raw build output of ncbi-vdb, including files that are not
             ;; installed.  Since we are building against an installed version of
             ;; ncbi-vdb, the following modifications are needed.
             (substitute* "setup/konfigure.perl"
               ;; Make the configure script look for the "ilib" directory of
               ;; "ncbi-vdb" without first checking for the existence of a
               ;; matching library in its "lib" directory.
               (("^            my \\$f = File::Spec->catdir\\(\\$libdir, \\$lib\\);")
                "my $f = File::Spec->catdir($ilibdir, $ilib);")
               ;; Look for interface libraries in ncbi-vdb's "ilib" directory.
               (("my \\$ilibdir = File::Spec->catdir\\(\\$builddir, 'ilib'\\);")
                "my $ilibdir = File::Spec->catdir($dir, 'ilib');"))

             ;; Dynamic linking
             (substitute* "tools/copycat/Makefile"
               (("smagic-static") "lmagic"))
             (substitute* "tools/driver-tool/utf8proc/Makefile"
               (("CC\\?=gcc") "myCC=gcc")
               (("\\(CC\\)") "(myCC)"))

             ;; The 'configure' script doesn't recognize things like
             ;; '--enable-fast-install'.
             (invoke "./configure"
                     (string-append "--build-prefix=" (getcwd) "/build")
                     (string-append "--prefix=" (assoc-ref outputs "out"))
                     (string-append "--debug")
                     (string-append "--with-fuse-prefix="
                                    (assoc-ref inputs "fuse"))
                     (string-append "--with-magic-prefix="
                                    (assoc-ref inputs "libmagic"))
                     ;; TODO: building with libxml2 fails with linker errors
                     #;
                     (string-append "--with-xml2-prefix="
                                    (assoc-ref inputs "libxml2"))
                     (string-append "--with-ncbi-vdb-sources="
                                    (assoc-ref inputs "ncbi-vdb"))
                     (string-append "--with-ncbi-vdb-build="
                                    (assoc-ref inputs "ncbi-vdb"))
                     (string-append "--with-ngs-sdk-prefix="
                                    (assoc-ref inputs "ngs-sdk"))
                     (string-append "--with-hdf5-prefix="
                                    (assoc-ref inputs "hdf5")))
             #t)))))
    (native-inputs `(("perl" ,perl)))
    (inputs
     `(("ngs-sdk" ,ngs-sdk)
       ("ncbi-vdb" ,ncbi-vdb)
       ("libmagic" ,file)
       ("fuse" ,fuse)
       ("hdf5" ,hdf5-1.10)
       ("zlib" ,zlib)
       ("python" ,python-wrapper)))
    (home-page
     "https://trace.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?view=software")
    (synopsis "Tools and libraries for reading and writing sequencing data")
    (description
     "The SRA Toolkit from NCBI is a collection of tools and libraries for
reading of sequencing files from the Sequence Read Archive (SRA) database and
writing files into the .sra format.")
    (license license:public-domain)))

(define-public seqan
  (package
    (name "seqan")
    (version "2.4.0")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://github.com/seqan/seqan/releases/"
                                  "download/seqan-v" version
                                  "/seqan-library-" version ".tar.xz"))
              (sha256
               (base32
                "19a1rlxx03qy1i1iriicly68w64yjxbv24g9gdywnfmq998v35yx"))))
    ;; The documentation is 7.8MB and the includes are 3.6MB heavy, so it
    ;; makes sense to split the outputs.
    (outputs '("out" "doc"))
    (build-system trivial-build-system)
    (arguments
     `(#:modules ((guix build utils))
       #:builder
       (begin
         (use-modules (guix build utils))
         (let ((tar (assoc-ref %build-inputs "tar"))
               (xz  (assoc-ref %build-inputs "xz"))
               (out (assoc-ref %outputs "out"))
               (doc (assoc-ref %outputs "doc")))
           (setenv "PATH" (string-append tar "/bin:" xz "/bin"))
           (invoke "tar" "xvf" (assoc-ref %build-inputs "source"))
           (chdir (string-append "seqan-library-" ,version))
           (copy-recursively "include" (string-append out "/include"))
           (copy-recursively "share"  (string-append doc "/share"))
           #t))))
    (native-inputs
     `(("source" ,source)
       ("tar" ,tar)
       ("xz" ,xz)))
    (home-page "http://www.seqan.de")
    (synopsis "Library for nucleotide sequence analysis")
    (description
     "SeqAn is a C++ library of efficient algorithms and data structures for
the analysis of sequences with the focus on biological data.  It contains
algorithms and data structures for string representation and their
manipulation, online and indexed string search, efficient I/O of
bioinformatics file formats, sequence alignment, and more.")
    (license license:bsd-3)))

(define-public seqan-1
  (package (inherit seqan)
    (name "seqan")
    (version "1.4.2")
    (source (origin
              (method url-fetch)
              (uri (string-append "http://packages.seqan.de/seqan-library/"
                                  "seqan-library-" version ".tar.bz2"))
              (sha256
               (base32
                "05s3wrrwn50f81aklfm65i4a749zag1vr8z03k21xm0pdxy47yvp"))))
    ;; The documentation is 7.8MB and the includes are 3.6MB heavy, so it
    ;; makes sense to split the outputs.
    (outputs '("out" "doc"))
    (build-system trivial-build-system)
    (arguments
     `(#:modules ((guix build utils))
       #:builder
       (begin
         (use-modules (guix build utils))
         (let ((tar  (assoc-ref %build-inputs "tar"))
               (bzip (assoc-ref %build-inputs "bzip2"))
               (out  (assoc-ref %outputs "out"))
               (doc  (assoc-ref %outputs "doc")))
           (setenv "PATH" (string-append tar "/bin:" bzip "/bin"))
           (invoke "tar" "xvf" (assoc-ref %build-inputs "source"))
           (chdir (string-append "seqan-library-" ,version))
           (copy-recursively "include" (string-append out "/include"))
           (copy-recursively "share"  (string-append doc "/share"))
           #t))))
    (native-inputs
     `(("source" ,source)
       ("tar" ,tar)
       ("bzip2" ,bzip2)))))

(define-public seqmagick
  (package
    (name "seqmagick")
    (version "0.8.0")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "seqmagick" version))
       (sha256
        (base32
         "0pf98da7i59q47gwrbx0wjk6xlvbybiwphw80w7h4ydjj0579a2b"))))
    (build-system python-build-system)
    (inputs
     `(("python-biopython" ,python-biopython)))
    (native-inputs
     `(("python-nose" ,python-nose)))
    (home-page "https://github.com/fhcrc/seqmagick")
    (synopsis "Tools for converting and modifying sequence files")
    (description
     "Bioinformaticians often have to convert sequence files between formats
and do little manipulations on them, and it's not worth writing scripts for
that.  Seqmagick is a utility to expose the file format conversion in
BioPython in a convenient way.  Instead of having a big mess of scripts, there
is one that takes arguments.")
    (license license:gpl3)))

(define-public seqtk
  (package
    (name "seqtk")
    (version "1.3")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/lh3/seqtk")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1bfzlqa84b5s1qi22blmmw2s8xdyp9h9ydcq22pfjhh5gab3yz6l"))))
    (build-system gnu-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (replace 'check
           ;; There are no tests, so we just run a sanity check.
           (lambda _ (invoke "./seqtk" "seq") #t))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((bin (string-append (assoc-ref outputs "out") "/bin/")))
               (install-file "seqtk" bin)
               #t))))))
    (inputs
     `(("zlib" ,zlib)))
    (home-page "https://github.com/lh3/seqtk")
    (synopsis "Toolkit for processing biological sequences in FASTA/Q format")
    (description
     "Seqtk is a fast and lightweight tool for processing sequences in the
FASTA or FASTQ format.  It parses both FASTA and FASTQ files which can be
optionally compressed by gzip.")
      (license license:expat)))

(define-public snap-aligner
  (package
    (name "snap-aligner")
    (version "1.0beta.18")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/amplab/snap")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "01w3qq4wm07z73vky0cfwlmrbf50n3w722cxrlzxfi99mnb808d8"))))
    (build-system gnu-build-system)
    (arguments
     '(#:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (replace 'check (lambda _ (invoke "./unit_tests") #t))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin")))
               (install-file "snap-aligner" bin)
               (install-file "SNAPCommand" bin)
               #t))))))
    (native-inputs
     `(("zlib" ,zlib)))
    (home-page "http://snap.cs.berkeley.edu/")
    (synopsis "Short read DNA sequence aligner")
    (description
     "SNAP is a fast and accurate aligner for short DNA reads.  It is
optimized for modern read lengths of 100 bases or higher, and takes advantage
of these reads to align data quickly through a hash-based indexing scheme.")
    ;; 32-bit systems are not supported by the unpatched code.
    ;; Following the bug reports https://github.com/amplab/snap/issues/68 and
    ;; https://bugs.debian.org/cgi-bin/bugreport.cgi?bug=812378 we see that
    ;; systems without a lot of memory cannot make good use of this program.
    (supported-systems '("x86_64-linux"))
    (license license:asl2.0)))

(define-public sortmerna
  (package
    (name "sortmerna")
    (version "2.1b")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/biocore/sortmerna")
             (commit version)))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "0j3mbz4n25738yijmjbr5r4fyvkgm8v5vn3sshyfvmyqf5q9byqf"))))
    (build-system gnu-build-system)
    (outputs '("out"      ;for binaries
               "db"))     ;for sequence databases
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out   (assoc-ref outputs "out"))
                    (bin   (string-append out "/bin"))
                    (db    (assoc-ref outputs "db"))
                    (share
                     (string-append db "/share/sortmerna/rRNA_databases")))
               (install-file "sortmerna" bin)
               (install-file "indexdb_rna" bin)
               (for-each (lambda (file)
                           (install-file file share))
                         (find-files "rRNA_databases" ".*fasta"))
               #t))))))
    (inputs
     `(("zlib" ,zlib)))
    (home-page "https://bioinfo.lifl.fr/RNA/sortmerna/")
    (synopsis "Biological sequence analysis tool for NGS reads")
    (description
     "SortMeRNA is a biological sequence analysis tool for filtering, mapping
and operational taxonomic unit (OTU) picking of next generation
sequencing (NGS) reads.  The core algorithm is based on approximate seeds and
allows for fast and sensitive analyses of nucleotide sequences.  The main
application of SortMeRNA is filtering rRNA from metatranscriptomic data.")
    ;; The source includes x86 specific code
    (supported-systems '("x86_64-linux" "i686-linux"))
    (license license:lgpl3)))

(define-public star
  (package
    (name "star")
    (version "2.7.3a")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/alexdobin/STAR")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1hgiqw5qhs0pc1xazzihcfd92na02xyq2kb469z04y1v51kpvvjq"))
              (modules '((guix build utils)))
              (snippet
               '(begin
                  (substitute* "source/Makefile"
                    (("/bin/rm") "rm"))
                  ;; Remove pre-built binaries and bundled htslib sources.
                  (delete-file-recursively "bin/MacOSX_x86_64")
                  (delete-file-recursively "bin/Linux_x86_64")
                  (delete-file-recursively "bin/Linux_x86_64_static")
                  (delete-file-recursively "source/htslib")
                  #t))))
    (build-system gnu-build-system)
    (arguments
     '(#:tests? #f ;no check target
       #:make-flags '("STAR")
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'enter-source-dir
           (lambda _ (chdir "source") #t))
         (add-after 'enter-source-dir 'make-reproducible
           (lambda _
             (substitute* "Makefile"
               (("(COMPILATION_TIME_PLACE=\")(.*)(\")" _ pre mid post)
                (string-append pre "Built with Guix" post)))
             #t))
         ;; See https://github.com/alexdobin/STAR/pull/562
         (add-after 'enter-source-dir 'add-missing-header
           (lambda _
             (substitute* "SoloReadFeature_inputRecords.cpp"
               (("#include \"binarySearch2.h\"" h)
                (string-append h "\n#include <math.h>")))
             #t))
         (add-after 'enter-source-dir 'do-not-use-bundled-htslib
           (lambda _
             (substitute* "Makefile"
               (("(Depend.list: \\$\\(SOURCES\\) parametersDefault\\.xxd) htslib"
                 _ prefix) prefix))
             (substitute* '("BAMfunctions.cpp"
                            "signalFromBAM.h"
                            "bam_cat.h"
                            "bam_cat.c"
                            "STAR.cpp"
                            "bamRemoveDuplicates.cpp")
               (("#include \"htslib/([^\"]+\\.h)\"" _ header)
                (string-append "#include <" header ">")))
             (substitute* "IncludeDefine.h"
               (("\"htslib/(htslib/[^\"]+.h)\"" _ header)
                (string-append "<" header ">")))
             #t))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((bin (string-append (assoc-ref outputs "out") "/bin/")))
               (install-file "STAR" bin))
             #t))
         (delete 'configure))))
    (native-inputs
     `(("xxd" ,xxd)))
    (inputs
     `(("htslib" ,htslib)
       ("zlib" ,zlib)))
    (home-page "https://github.com/alexdobin/STAR")
    (synopsis "Universal RNA-seq aligner")
    (description
     "The Spliced Transcripts Alignment to a Reference (STAR) software is
based on a previously undescribed RNA-seq alignment algorithm that uses
sequential maximum mappable seed search in uncompressed suffix arrays followed
by seed clustering and stitching procedure.  In addition to unbiased de novo
detection of canonical junctions, STAR can discover non-canonical splices and
chimeric (fusion) transcripts, and is also capable of mapping full-length RNA
sequences.")
    ;; Only 64-bit systems are supported according to the README.
    (supported-systems '("x86_64-linux" "mips64el-linux"))
    ;; STAR is licensed under GPLv3 or later; htslib is MIT-licensed.
    (license license:gpl3+)))

(define-public starlong
  (package (inherit star)
    (name "starlong")
    (arguments
     (substitute-keyword-arguments (package-arguments star)
       ((#:make-flags flags)
        `(list "STARlong"))
       ((#:phases phases)
        `(modify-phases ,phases
           ;; Allow extra long sequence reads.
           (add-after 'unpack 'make-extra-long
             (lambda _
               (substitute* "source/IncludeDefine.h"
                 (("(#define DEF_readNameLengthMax ).*" _ match)
                  (string-append match "900000\n")))
               #t))
           (replace 'install
             (lambda* (#:key outputs #:allow-other-keys)
               (let ((bin (string-append (assoc-ref outputs "out") "/bin/")))
                 (install-file "STARlong" bin))
               #t))))))))

(define-public subread
  (package
    (name "subread")
    (version "1.6.0")
    (source (origin
              (method url-fetch)
              (uri (string-append "mirror://sourceforge/subread/subread-"
                                  version "/subread-" version "-source.tar.gz"))
              (sha256
               (base32
                "0ah0n4jx6ksk2m2j7xk385x2qzmk1y4rfc6a4mfrdqrlq721w99i"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ;no "check" target
      ;; The CC and CCFLAGS variables are set to contain a lot of x86_64
      ;; optimizations by default, so we override these flags such that x86_64
      ;; flags are only added when the build target is an x86_64 system.
       #:make-flags
       (list (let ((system ,(or (%current-target-system)
                                (%current-system)))
                   (flags '("-ggdb" "-fomit-frame-pointer"
                            "-ffast-math" "-funroll-loops"
                            "-fmessage-length=0"
                            "-O9" "-Wall" "-DMAKE_FOR_EXON"
                            "-DMAKE_STANDALONE"
                            "-DSUBREAD_VERSION=\\\"${SUBREAD_VERSION}\\\""))
                   (flags64 '("-mmmx" "-msse" "-msse2" "-msse3")))
               (if (string-prefix? "x86_64" system)
                   (string-append "CCFLAGS=" (string-join (append flags flags64)))
                   (string-append "CCFLAGS=" (string-join flags))))
             "-f" "Makefile.Linux"
             "CC=gcc ${CCFLAGS}")
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'enter-dir
           (lambda _ (chdir "src") #t))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((bin (string-append (assoc-ref outputs "out") "/bin/")))
               (mkdir-p bin)
               (copy-recursively "../bin" bin))
             #t))
         ;; no "configure" script
         (delete 'configure))))
    (inputs `(("zlib" ,zlib)))
    (home-page "http://bioinf.wehi.edu.au/subread-package/")
    (synopsis "Tool kit for processing next-gen sequencing data")
    (description
     "The subread package contains the following tools: subread aligner, a
general-purpose read aligner; subjunc aligner: detecting exon-exon junctions
and mapping RNA-seq reads; featureCounts: counting mapped reads for genomic
features; exactSNP: a SNP caller that discovers SNPs by testing signals
against local background noises.")
    (license license:gpl3+)))

(define-public stringtie
  (package
    (name "stringtie")
    (version "1.2.1")
    (source (origin
              (method url-fetch)
              (uri (string-append "http://ccb.jhu.edu/software/stringtie/dl/"
                                  "stringtie-" version ".tar.gz"))
              (sha256
               (base32
                "1cqllsc1maq4kh92isi8yadgzbmnf042hlnalpk3y59aph1z3bfz"))
              (modules '((guix build utils)))
              (snippet
               '(begin
                  (delete-file-recursively "samtools-0.1.18")
                  #t))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ;no test suite
       #:phases
       (modify-phases %standard-phases
         ;; no configure script
         (delete 'configure)
         (add-before 'build 'use-system-samtools
           (lambda _
             (substitute* "Makefile"
               (("stringtie: \\$\\{BAM\\}/libbam\\.a")
                "stringtie: "))
             (substitute* '("gclib/GBam.h"
                            "gclib/GBam.cpp")
               (("#include \"(bam|sam|kstring).h\"" _ header)
                (string-append "#include <samtools/" header ".h>")))
             #t))
         (add-after 'unpack 'remove-duplicate-typedef
           (lambda _
             ;; This typedef conflicts with the typedef in
             ;; glibc-2.25/include/bits/types.h
             (substitute* "gclib/GThreads.h"
               (("typedef long long __intmax_t;") ""))
             #t))
         (replace 'install
          (lambda* (#:key outputs #:allow-other-keys)
            (let ((bin (string-append (assoc-ref outputs "out") "/bin/")))
              (install-file "stringtie" bin)
              #t))))))
    (inputs
     `(("samtools" ,samtools-0.1)
       ("zlib" ,zlib)))
    (home-page "http://ccb.jhu.edu/software/stringtie/")
    (synopsis "Transcript assembly and quantification for RNA-Seq data")
    (description
     "StringTie is a fast and efficient assembler of RNA-Seq sequence
alignments into potential transcripts.  It uses a novel network flow algorithm
as well as an optional de novo assembly step to assemble and quantitate
full-length transcripts representing multiple splice variants for each gene
locus.  Its input can include not only the alignments of raw reads used by
other transcript assemblers, but also alignments of longer sequences that have
been assembled from those reads.  To identify differentially expressed genes
between experiments, StringTie's output can be processed either by the
Cuffdiff or Ballgown programs.")
    (license license:artistic2.0)))

(define-public taxtastic
  (package
    (name "taxtastic")
    (version "0.8.11")
    (source (origin
              ;; The Pypi version does not include tests.
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/fhcrc/taxtastic")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1sv8mkg64jn7zdwf1jj71c16686yrwxk0apb1l8sjszy9p166g0p"))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'prepare-directory
           (lambda _
             ;; The git checkout must be writable for tests.
             (for-each make-file-writable (find-files "."))
             ;; This test fails, but the error is not caught by the test
             ;; framework, so the tests fail...
             (substitute* "tests/test_taxit.py"
               (("self.cmd_fails\\(''\\)")
                "self.cmd_fails('nothing')"))
             ;; This version file is expected to be created with git describe.
             (mkdir-p "taxtastic/data")
             (with-output-to-file "taxtastic/data/ver"
               (lambda () (display ,version)))
             #t))
         (add-after 'unpack 'python37-compatibility
           (lambda _
             (substitute* "taxtastic/utils.py"
               (("import csv") "import csv, errno")
               (("os.errno") "errno"))
             #t))
         (replace 'check
           ;; Note, this fails to run with "-v" as it tries to write to a
           ;; closed output stream.
           (lambda _ (invoke "python" "-m" "unittest") #t)))))
    (propagated-inputs
     `(("python-sqlalchemy" ,python-sqlalchemy)
       ("python-decorator" ,python-decorator)
       ("python-biopython" ,python-biopython)
       ("python-pandas" ,python-pandas)
       ("python-psycopg2" ,python-psycopg2)
       ("python-fastalite" ,python-fastalite)
       ("python-pyyaml" ,python-pyyaml)
       ("python-six" ,python-six)
       ("python-jinja2" ,python-jinja2)
       ("python-dendropy" ,python-dendropy)))
    (home-page "https://github.com/fhcrc/taxtastic")
    (synopsis "Tools for taxonomic naming and annotation")
    (description
     "Taxtastic is software written in python used to build and maintain
reference packages i.e. collections of reference trees, reference alignments,
profiles, and associated taxonomic information.")
    (license license:gpl3+)))

(define-public vcftools
  (package
    (name "vcftools")
    (version "0.1.16")
    (source (origin
              (method url-fetch)
              (uri (string-append
                    "https://github.com/vcftools/vcftools/releases/download/v"
                    version "/vcftools-" version ".tar.gz"))
              (sha256
               (base32
                "1qqlx7flfv7axrjwkaz6njkscsl1d0jw98ns8d8bh1n1hd1pgz6v"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; no "check" target
       #:make-flags (list
                     "CFLAGS=-O2" ; override "-m64" flag
                     (string-append "PREFIX=" (assoc-ref %outputs "out"))
                     (string-append "MANDIR=" (assoc-ref %outputs "out")
                                    "/share/man/man1"))))
    (native-inputs
     `(("pkg-config" ,pkg-config)))
    (inputs
     `(("perl" ,perl)
       ("zlib" ,zlib)))
    (home-page "https://vcftools.github.io/")
    (synopsis "Tools for working with VCF files")
    (description
     "VCFtools is a program package designed for working with VCF files, such
as those generated by the 1000 Genomes Project.  The aim of VCFtools is to
provide easily accessible methods for working with complex genetic variation
data in the form of VCF files.")
    ;; The license is declared as LGPLv3 in the README and
    ;; at https://vcftools.github.io/license.html
    (license license:lgpl3)))

(define-public infernal
  (package
    (name "infernal")
    (version "1.1.3")
    (source (origin
              (method url-fetch)
              (uri (string-append "http://eddylab.org/software/infernal/"
                                  "infernal-" version ".tar.gz"))
              (sha256
               (base32
                "0pm8bm3s6nfa0av4x6m6h27lsg12b3lz3jm0fyh1mc77l2isd61v"))))
    (build-system gnu-build-system)
    (native-inputs
     `(("perl" ,perl)
       ("python" ,python))) ; for tests
    (home-page "http://eddylab.org/infernal/")
    (synopsis "Inference of RNA alignments")
    (description "Infernal (\"INFERence of RNA ALignment\") is a tool for
searching DNA sequence databases for RNA structure and sequence similarities.
It is an implementation of a special case of profile stochastic context-free
grammars called @dfn{covariance models} (CMs).  A CM is like a sequence
profile, but it scores a combination of sequence consensus and RNA secondary
structure consensus, so in many cases, it is more capable of identifying RNA
homologs that conserve their secondary structure more than their primary
sequence.")
    ;; Infernal 1.1.3 requires VMX or SSE capability for parallel instructions.
    (supported-systems '("i686-linux" "x86_64-linux"))
    (license license:bsd-3)))

(define-public r-scde
  (package
    (name "r-scde")
    (version "1.99.2")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/hms-dbmi/scde")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "10na2gyka24mszdxf92wz9h2c13hdf1ww30c68gfsw53lvvhhhxb"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-rcpp" ,r-rcpp)
       ("r-rcpparmadillo" ,r-rcpparmadillo)
       ("r-mgcv" ,r-mgcv)
       ("r-rook" ,r-rook)
       ("r-rjson" ,r-rjson)
       ("r-cairo" ,r-cairo)
       ("r-rcolorbrewer" ,r-rcolorbrewer)
       ("r-edger" ,r-edger)
       ("r-quantreg" ,r-quantreg)
       ("r-nnet" ,r-nnet)
       ("r-rmtstat" ,r-rmtstat)
       ("r-extremes" ,r-extremes)
       ("r-pcamethods" ,r-pcamethods)
       ("r-biocparallel" ,r-biocparallel)
       ("r-flexmix" ,r-flexmix)))
    (home-page "https://hms-dbmi.github.io/scde/")
    (synopsis "R package for analyzing single-cell RNA-seq data")
    (description "The SCDE package implements a set of statistical methods for
analyzing single-cell RNA-seq data.  SCDE fits individual error models for
single-cell RNA-seq measurements.  These models can then be used for
assessment of differential expression between groups of cells, as well as
other types of analysis.  The SCDE package also contains the pagoda framework
which applies pathway and gene set overdispersion analysis to identify aspects
of transcriptional heterogeneity among single cells.")
    ;; See https://github.com/hms-dbmi/scde/issues/38
    (license license:gpl2)))

(define-public r-centipede
  (package
    (name "r-centipede")
    (version "1.2")
    (source (origin
              (method url-fetch)
              (uri (string-append "http://download.r-forge.r-project.org/"
                                  "src/contrib/CENTIPEDE_" version ".tar.gz"))
              (sha256
               (base32
                "1hsx6qgwr0i67fhy9257zj7s0ppncph2hjgbia5nn6nfmj0ax6l9"))))
    (build-system r-build-system)
    (home-page "http://centipede.uchicago.edu/")
    (synopsis "Predict transcription factor binding sites")
    (description
     "CENTIPEDE applies a hierarchical Bayesian mixture model to infer regions
of the genome that are bound by particular transcription factors.  It starts
by identifying a set of candidate binding sites, and then aims to classify the
sites according to whether each site is bound or not bound by a transcription
factor.  CENTIPEDE is an unsupervised learning algorithm that discriminates
between two different types of motif instances using as much relevant
information as possible.")
    (license (list license:gpl2+ license:gpl3+))))

(define-public r-genefilter
  (package
    (name "r-genefilter")
    (version "1.70.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "genefilter" version))
       (sha256
        (base32
         "1sbbrnq6p90fri0ik6aq2zw26kasw63nyiy7xkzrj6vgyq7x258g"))))
    (build-system r-build-system)
    (native-inputs
     `(("gfortran" ,gfortran)
       ("r-knitr" ,r-knitr)))
    (propagated-inputs
     `(("r-annotate" ,r-annotate)
       ("r-annotationdbi" ,r-annotationdbi)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-survival" ,r-survival)))
    (home-page "https://bioconductor.org/packages/genefilter")
    (synopsis "Filter genes from high-throughput experiments")
    (description
     "This package provides basic functions for filtering genes from
high-throughput sequencing experiments.")
    (license license:artistic2.0)))

(define-public r-deseq2
  (package
    (name "r-deseq2")
    (version "1.28.1")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "DESeq2" version))
       (sha256
        (base32
         "0xh12c2skr0bbv893p05gvbismkcnqw8zwh7yz4wmycgajfzg2pp"))))
    (properties `((upstream-name . "DESeq2")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-biocparallel" ,r-biocparallel)
       ("r-genefilter" ,r-genefilter)
       ("r-geneplotter" ,r-geneplotter)
       ("r-genomicranges" ,r-genomicranges)
       ("r-ggplot2" ,r-ggplot2)
       ("r-iranges" ,r-iranges)
       ("r-locfit" ,r-locfit)
       ("r-rcpp" ,r-rcpp)
       ("r-rcpparmadillo" ,r-rcpparmadillo)
       ("r-s4vectors" ,r-s4vectors)
       ("r-summarizedexperiment" ,r-summarizedexperiment)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/DESeq2")
    (synopsis "Differential gene expression analysis")
    (description
     "This package provides functions to estimate variance-mean dependence in
count data from high-throughput nucleotide sequencing assays and test for
differential expression based on a model using the negative binomial
distribution.")
    (license license:lgpl3+)))

(define-public r-dexseq
  (package
    (name "r-dexseq")
    (version "1.34.1")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "DEXSeq" version))
       (sha256
        (base32
         "1m03awaw06mfv5gszq23k5apsqqzjqa5rcwp20y4xbpl7bywpsyl"))))
    (properties `((upstream-name . "DEXSeq")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-biocparallel" ,r-biocparallel)
       ("r-biomart" ,r-biomart)
       ("r-deseq2" ,r-deseq2)
       ("r-genefilter" ,r-genefilter)
       ("r-geneplotter" ,r-geneplotter)
       ("r-genomicranges" ,r-genomicranges)
       ("r-hwriter" ,r-hwriter)
       ("r-iranges" ,r-iranges)
       ("r-rcolorbrewer" ,r-rcolorbrewer)
       ("r-rsamtools" ,r-rsamtools)
       ("r-s4vectors" ,r-s4vectors)
       ("r-statmod" ,r-statmod)
       ("r-stringr" ,r-stringr)
       ("r-summarizedexperiment" ,r-summarizedexperiment)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/DEXSeq")
    (synopsis "Inference of differential exon usage in RNA-Seq")
    (description
     "This package is focused on finding differential exon usage using RNA-seq
exon counts between samples with different experimental designs.  It provides
functions that allows the user to make the necessary statistical tests based
on a model that uses the negative binomial distribution to estimate the
variance between biological replicates and generalized linear models for
testing.  The package also provides functions for the visualization and
exploration of the results.")
    (license license:gpl3+)))

(define-public r-annotationforge
  (package
    (name "r-annotationforge")
    (version "1.30.1")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "AnnotationForge" version))
       (sha256
        (base32
         "1a2155jxbwc6qr3dcyvp850grhdr7czc7bs3s87ff4sgdl0jp3jw"))))
    (properties
     `((upstream-name . "AnnotationForge")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-dbi" ,r-dbi)
       ("r-rcurl" ,r-rcurl)
       ("r-rsqlite" ,r-rsqlite)
       ("r-s4vectors" ,r-s4vectors)
       ("r-xml" ,r-xml)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/AnnotationForge")
    (synopsis "Code for building annotation database packages")
    (description
     "This package provides code for generating Annotation packages and their
databases.  Packages produced are intended to be used with AnnotationDbi.")
    (license license:artistic2.0)))

(define-public r-rbgl
  (package
    (name "r-rbgl")
    (version "1.64.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "RBGL" version))
       (sha256
        (base32
         "079599a6xn2i7snfn2vgshkw0c00rrfhj44pvi03ap8id29bkayy"))))
    (properties `((upstream-name . "RBGL")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-bh" ,r-bh)
       ("r-graph" ,r-graph)))
    (home-page "https://www.bioconductor.org/packages/RBGL")
    (synopsis "Interface to the Boost graph library")
    (description
     "This package provides a fairly extensive and comprehensive interface to
the graph algorithms contained in the Boost library.")
    (license license:artistic2.0)))

(define-public r-gseabase
  (package
    (name "r-gseabase")
    (version "1.50.1")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "GSEABase" version))
       (sha256
        (base32
         "1k4faj53cwvqijad8cf7fcghzxcv9shlbpl8n73bsncc8k192y2j"))))
    (properties `((upstream-name . "GSEABase")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotate" ,r-annotate)
       ("r-annotationdbi" ,r-annotationdbi)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-graph" ,r-graph)
       ("r-xml" ,r-xml)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/GSEABase")
    (synopsis "Gene set enrichment data structures and methods")
    (description
     "This package provides classes and methods to support @dfn{Gene Set
Enrichment Analysis} (GSEA).")
    (license license:artistic2.0)))

(define-public r-category
  (package
    (name "r-category")
    (version "2.54.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "Category" version))
       (sha256
        (base32
         "1grspdzk5a4vidnxwcd1jmy1vcn494aydsp3vydx235yv7iqac1b"))))
    (properties `((upstream-name . "Category")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotate" ,r-annotate)
       ("r-annotationdbi" ,r-annotationdbi)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-genefilter" ,r-genefilter)
       ("r-graph" ,r-graph)
       ("r-gseabase" ,r-gseabase)
       ("r-matrix" ,r-matrix)
       ("r-rbgl" ,r-rbgl)
       ("r-dbi" ,r-dbi)))
    (home-page "https://bioconductor.org/packages/Category")
    (synopsis "Category analysis")
    (description
     "This package provides a collection of tools for performing category
analysis.")
    (license license:artistic2.0)))

(define-public r-gostats
  (package
    (name "r-gostats")
    (version "2.54.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "GOstats" version))
       (sha256
        (base32
         "00q39cyv4l28r6s9gjnd0qhl7h80vmwp4lpmchisqzj44xzyics9"))))
    (properties `((upstream-name . "GOstats")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotate" ,r-annotate)
       ("r-annotationdbi" ,r-annotationdbi)
       ("r-annotationforge" ,r-annotationforge)
       ("r-biobase" ,r-biobase)
       ("r-category" ,r-category)
       ("r-go-db" ,r-go-db)
       ("r-graph" ,r-graph)
       ("r-rgraphviz" ,r-rgraphviz)
       ("r-rbgl" ,r-rbgl)))
    (home-page "https://bioconductor.org/packages/GOstats")
    (synopsis "Tools for manipulating GO and microarrays")
    (description
     "This package provides a set of tools for interacting with GO and
microarray data.  A variety of basic manipulation tools for graphs, hypothesis
testing and other simple calculations.")
    (license license:artistic2.0)))

(define-public r-shortread
  (package
    (name "r-shortread")
    (version "1.46.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "ShortRead" version))
       (sha256
        (base32
         "0l4kdln69y6yhln0xiv2jmpxg05fjcglln406p43a2bqvk2lr03d"))))
    (properties `((upstream-name . "ShortRead")))
    (build-system r-build-system)
    (inputs
     `(("zlib" ,zlib)))
    (propagated-inputs
     `(("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-biocparallel" ,r-biocparallel)
       ("r-biostrings" ,r-biostrings)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicalignments" ,r-genomicalignments)
       ("r-genomicranges" ,r-genomicranges)
       ("r-hwriter" ,r-hwriter)
       ("r-iranges" ,r-iranges)
       ("r-lattice" ,r-lattice)
       ("r-latticeextra" ,r-latticeextra)
       ("r-rsamtools" ,r-rsamtools)
       ("r-s4vectors" ,r-s4vectors)
       ("r-xvector" ,r-xvector)
       ("r-zlibbioc" ,r-zlibbioc)))
    (home-page "https://bioconductor.org/packages/ShortRead")
    (synopsis "FASTQ input and manipulation tools")
    (description
     "This package implements sampling, iteration, and input of FASTQ files.
It includes functions for filtering and trimming reads, and for generating a
quality assessment report.  Data are represented as
@code{DNAStringSet}-derived objects, and easily manipulated for a diversity of
purposes.  The package also contains legacy support for early single-end,
ungapped alignment formats.")
    (license license:artistic2.0)))

(define-public r-systempiper
  (package
    (name "r-systempiper")
    (version "1.22.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "systemPipeR" version))
       (sha256
        (base32
         "01ilhlrvy28jfdyxjria4024yryj5zgympgqznw17g3y3az78kk2"))))
    (properties `((upstream-name . "systemPipeR")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotate" ,r-annotate)
       ("r-assertthat" ,r-assertthat)
       ("r-batchtools" ,r-batchtools)
       ("r-biostrings" ,r-biostrings)
       ("r-deseq2" ,r-deseq2)
       ("r-dot" ,r-dot)
       ("r-edger" ,r-edger)
       ("r-genomicfeatures" ,r-genomicfeatures)
       ("r-genomicranges" ,r-genomicranges)
       ("r-ggplot2" ,r-ggplot2)
       ("r-go-db" ,r-go-db)
       ("r-gostats" ,r-gostats)
       ("r-limma" ,r-limma)
       ("r-magrittr" ,r-magrittr)
       ("r-pheatmap" ,r-pheatmap)
       ("r-rjson" ,r-rjson)
       ("r-rsamtools" ,r-rsamtools)
       ("r-rsvg" ,r-rsvg)
       ("r-shortread" ,r-shortread)
       ("r-stringr" ,r-stringr)
       ("r-summarizedexperiment" ,r-summarizedexperiment)
       ("r-yaml" ,r-yaml)
       ("r-variantannotation" ,r-variantannotation)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://github.com/tgirke/systemPipeR")
    (synopsis "Next generation sequencing workflow and reporting environment")
    (description
     "This R package provides tools for building and running automated
end-to-end analysis workflows for a wide range of @dfn{next generation
sequence} (NGS) applications such as RNA-Seq, ChIP-Seq, VAR-Seq and Ribo-Seq.
Important features include a uniform workflow interface across different NGS
applications, automated report generation, and support for running both R and
command-line software, such as NGS aligners or peak/variant callers, on local
computers or compute clusters.  Efficient handling of complex sample sets and
experimental designs is facilitated by a consistently implemented sample
annotation infrastructure.")
    (license license:artistic2.0)))

(define-public r-grohmm
  (package
    (name "r-grohmm")
    (version "1.22.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "groHMM" version))
       (sha256
        (base32
         "04z9qq1xwdsaxbqhyld37w0ybvzly9pc1hcyrnwdbyjwd7n1fncb"))))
    (properties `((upstream-name . "groHMM")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicalignments" ,r-genomicalignments)
       ("r-genomicranges" ,r-genomicranges)
       ("r-iranges" ,r-iranges)
       ("r-mass" ,r-mass)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-s4vectors" ,r-s4vectors)))
    (home-page "https://github.com/Kraus-Lab/groHMM")
    (synopsis "GRO-seq analysis pipeline")
    (description
     "This package provides a pipeline for the analysis of GRO-seq data.")
    (license license:gpl3+)))

(define-public vsearch
  (package
    (name "vsearch")
    (version "2.9.1")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/torognes/vsearch")
             (commit (string-append "v" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "0vhrpjfdf75ba04b24xknp41790cvcgwl0vgpy7qbzj5xh2521ss"))
       (patches (search-patches "vsearch-unbundle-cityhash.patch"))
       (snippet
        '(begin
           ;; Remove bundled cityhash sources.  The vsearch source is adjusted
           ;; for this in the patch.
           (delete-file "src/city.h")
           (delete-file "src/citycrc.h")
           (delete-file "src/city.cc")
           #t))))
    (build-system gnu-build-system)
    (inputs
     `(("zlib" ,zlib)
       ("bzip2" ,bzip2)
       ("cityhash" ,cityhash)))
    (native-inputs
     `(("autoconf" ,autoconf)
       ("automake" ,automake)))
    (synopsis "Sequence search tools for metagenomics")
    (description
     "VSEARCH supports DNA sequence searching, clustering, chimera detection,
dereplication, pairwise alignment, shuffling, subsampling, sorting and
masking.  The tool takes advantage of parallelism in the form of SIMD
vectorization as well as multiple threads to perform accurate alignments at
high speed.  VSEARCH uses an optimal global aligner (full dynamic programming
Needleman-Wunsch).")
    (home-page "https://github.com/torognes/vsearch")
    ;; vsearch uses non-portable SSE intrinsics so building fails on other
    ;; platforms.
    (supported-systems '("x86_64-linux"))
    ;; Dual licensed; also includes public domain source.
    (license (list license:gpl3 license:bsd-2))))

(define-public pardre
  (package
    (name "pardre")
    ;; The source of 1.1.5 changed in place, so we append "-1" to the version.
    (version "1.1.5-1")
    (source
     (origin
       (method url-fetch)
       (uri (string-append "mirror://sourceforge/pardre/ParDRe-rel"
                           "1.1.5" ".tar.gz"))
       (sha256
        (base32
         "17j73nc0viq4f6qj50nrndsrif5d6b71q8fl87m54psiv0ilns2b"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; no tests included
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((bin (string-append (assoc-ref outputs "out") "/bin")))
               (install-file "ParDRe" bin)
               #t))))))
    (inputs
     `(("openmpi" ,openmpi)
       ("zlib" ,zlib)))
    (synopsis "Parallel tool to remove duplicate DNA reads")
    (description
     "ParDRe is a parallel tool to remove duplicate genetic sequence reads.
Duplicate reads can be seen as identical or nearly identical sequences with
some mismatches.  This tool lets users avoid the analysis of unnecessary
reads, reducing the time of subsequent procedures with the
dataset (e.g. assemblies, mappings, etc.).  The tool is implemented with MPI
in order to exploit the parallel capabilities of multicore clusters.  It is
faster than multithreaded counterparts (end of 2015) for the same number of
cores and, thanks to the message-passing technology, it can be executed on
clusters.")
    (home-page "https://sourceforge.net/projects/pardre/")
    (license license:gpl3+)))

(define-public ruby-bio-kseq
  (package
    (name "ruby-bio-kseq")
    (version "0.0.2")
    (source
     (origin
       (method url-fetch)
       (uri (rubygems-uri "bio-kseq" version))
       (sha256
        (base32
         "1xyaha46khb5jc6wzkbf7040jagac49jbimn0vcrzid0j8jdikrz"))))
    (build-system ruby-build-system)
    (arguments
     `(#:test-target "spec"))
    (native-inputs
     `(("bundler" ,bundler)
       ("ruby-rspec" ,ruby-rspec)
       ("ruby-rake-compiler" ,ruby-rake-compiler)))
    (inputs
     `(("zlib" ,zlib)))
    (synopsis "Ruby bindings for the kseq.h FASTA/Q parser")
    (description
     "@code{Bio::Kseq} provides ruby bindings to the @code{kseq.h} FASTA and
FASTQ parsing code.  It provides a fast iterator over sequences and their
quality scores.")
    (home-page "https://github.com/gusevfe/bio-kseq")
    (license license:expat)))

(define-public bio-locus
  (package
    (name "bio-locus")
    (version "0.0.7")
    (source
     (origin
       (method url-fetch)
       (uri (rubygems-uri "bio-locus" version))
       (sha256
        (base32
         "02vmrxyimkj9sahsp4zhfhnmbvz6dbbqz1y01vglf8cbwvkajfl0"))))
    (build-system ruby-build-system)
    (native-inputs
     `(("ruby-rspec" ,ruby-rspec)))
    (synopsis "Tool for fast querying of genome locations")
    (description
     "Bio-locus is a tabix-like tool for fast querying of genome
locations.  Many file formats in bioinformatics contain records that
start with a chromosome name and a position for a SNP, or a start-end
position for indels.  Bio-locus allows users to store this chr+pos or
chr+pos+alt information in a database.")
    (home-page "https://github.com/pjotrp/bio-locus")
    (license license:expat)))

(define-public bio-blastxmlparser
  (package
    (name "bio-blastxmlparser")
    (version "2.0.4")
    (source (origin
              (method url-fetch)
              (uri (rubygems-uri "bio-blastxmlparser" version))
              (sha256
               (base32
                "1wf4qygcmdjgcqm6flmvsagfr1gs9lf63mj32qv3z1f481zc5692"))))
    (build-system ruby-build-system)
    (propagated-inputs
     `(("ruby-bio-logger" ,ruby-bio-logger)
       ("ruby-nokogiri" ,ruby-nokogiri)))
    (inputs
     `(("ruby-rspec" ,ruby-rspec)))
    (synopsis "Fast big data BLAST XML parser and library")
    (description
     "Very fast parallel big-data BLAST XML file parser which can be used as
command line utility.  Use blastxmlparser to: Parse BLAST XML; filter output;
generate FASTA, JSON, YAML, RDF, JSON-LD, HTML, CSV, tabular output etc.")
    (home-page "https://github.com/pjotrp/blastxmlparser")
    (license license:expat)))

(define-public bioruby
  (package
    (name "bioruby")
    (version "1.5.2")
    (source
     (origin
       (method url-fetch)
       (uri (rubygems-uri "bio" version))
       (sha256
        (base32
         "1d56amdsjv1mag7m6gv2w0xij8hqx1v5xbdjsix8sp3yp36m7938"))))
    (build-system ruby-build-system)
    (propagated-inputs
     `(("ruby-libxml" ,ruby-libxml)))
    (native-inputs
     `(("which" ,which)))  ; required for test phase
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-before 'build 'patch-test-command
          (lambda _
            (substitute* '("test/functional/bio/test_command.rb")
              (("/bin/sh") (which "sh")))
            (substitute* '("test/functional/bio/test_command.rb")
              (("/bin/ls") (which "ls")))
            (substitute* '("test/functional/bio/test_command.rb")
              (("which") (which "which")))
            (substitute* '("test/functional/bio/test_command.rb",
                           "test/data/command/echoarg2.sh")
              (("/bin/echo") (which "echo")))
            #t)))))
    (synopsis "Ruby library, shell and utilities for bioinformatics")
    (description "BioRuby comes with a comprehensive set of Ruby development
tools and libraries for bioinformatics and molecular biology.  BioRuby has
components for sequence analysis, pathway analysis, protein modelling and
phylogenetic analysis; it supports many widely used data formats and provides
easy access to databases, external programs and public web services, including
BLAST, KEGG, GenBank, MEDLINE and GO.")
    (home-page "http://bioruby.org/")
    ;; Code is released under Ruby license, except for setup
    ;; (LGPLv2.1+) and scripts in samples (which have GPL2 and GPL2+)
    (license (list license:ruby license:lgpl2.1+ license:gpl2+ ))))

(define-public r-biocviews
  (package
    (name "r-biocviews")
    (version "1.56.2")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "biocViews" version))
              (sha256
               (base32
                "0kw0qfm1fw5yv2rbz6x23431rh0fnzj66f2bls7j285biyjmmx7w"))))
    (properties
     `((upstream-name . "biocViews")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biobase" ,r-biobase)
       ("r-biocmanager" ,r-biocmanager)
       ("r-graph" ,r-graph)
       ("r-rbgl" ,r-rbgl)
       ("r-rcurl" ,r-rcurl)
       ("r-xml" ,r-xml)
       ("r-runit" ,r-runit)))
    (home-page "https://bioconductor.org/packages/biocViews")
    (synopsis "Bioconductor package categorization helper")
    (description "The purpose of biocViews is to create HTML pages that
categorize packages in a Bioconductor package repository according to keywords,
also known as views, in a controlled vocabulary.")
    (license license:artistic2.0)))

(define-public r-biocstyle
  (package
    (name "r-biocstyle")
    (version "2.16.1")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "BiocStyle" version))
              (sha256
               (base32
                "09sz2bnbfbj8wz16k0q8xrvag9fldxqyp5vbg9pvd9skh28hv7c7"))))
    (properties
     `((upstream-name . "BiocStyle")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocmanager" ,r-biocmanager)
       ("r-bookdown" ,r-bookdown)
       ("r-knitr" ,r-knitr)
       ("r-rmarkdown" ,r-rmarkdown)
       ("r-yaml" ,r-yaml)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/BiocStyle")
    (synopsis "Bioconductor formatting styles")
    (description "This package provides standard formatting styles for
Bioconductor PDF and HTML documents.  Package vignettes illustrate use and
functionality.")
    (license license:artistic2.0)))

(define-public r-bioccheck
  (package
    (name "r-bioccheck")
    (version "1.24.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "BiocCheck" version))
              (sha256
               (base32
                "1p9ys18sn1crxw1iac2jdgqvwpb5hjd9nfxx0qn0ncrv0b550mny"))))
    (properties
     `((upstream-name . "BiocCheck")))
    (build-system r-build-system)
    (arguments
     '(#:phases
       (modify-phases %standard-phases
         ;; This package can be used by calling BiocCheck(<package>) from
         ;; within R, or by running R CMD BiocCheck <package>.  This phase
         ;; makes sure the latter works.  For this to work, the BiocCheck
         ;; script must be somewhere on the PATH (not the R bin directory).
         (add-after 'install 'install-bioccheck-subcommand
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (dest-dir (string-append out "/bin"))
                    (script-dir
                     (string-append out "/site-library/BiocCheck/script/")))
               (mkdir-p dest-dir)
               (symlink (string-append script-dir "/checkBadDeps.R")
                        (string-append dest-dir "/checkBadDeps.R"))
               (symlink (string-append script-dir "/BiocCheck")
                        (string-append dest-dir "/BiocCheck")))
             #t)))))
    (propagated-inputs
     `(("r-codetools" ,r-codetools)
       ("r-graph" ,r-graph)
       ("r-httr" ,r-httr)
       ("r-knitr" ,r-knitr)
       ("r-optparse" ,r-optparse)
       ("r-biocmanager" ,r-biocmanager)
       ("r-biocviews" ,r-biocviews)
       ("r-stringdist" ,r-stringdist)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/BiocCheck")
    (synopsis "Executes Bioconductor-specific package checks")
    (description "This package contains tools to perform additional quality
checks on R packages that are to be submitted to the Bioconductor repository.")
    (license license:artistic2.0)))

(define-public r-s4vectors
  (package
    (name "r-s4vectors")
    (version "0.26.1")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "S4Vectors" version))
              (sha256
               (base32
                "1ddr3ngyczx332zw9ai1a6h7442lgrbfcj8vrhvbkdkzqwj14xmb"))))
    (properties
     `((upstream-name . "S4Vectors")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)))
    (home-page "https://bioconductor.org/packages/S4Vectors")
    (synopsis "S4 implementation of vectors and lists")
    (description
     "The S4Vectors package defines the @code{Vector} and @code{List} virtual
classes and a set of generic functions that extend the semantic of ordinary
vectors and lists in R.  Package developers can easily implement vector-like
or list-like objects as concrete subclasses of @code{Vector} or @code{List}.
In addition, a few low-level concrete subclasses of general interest (e.g.
@code{DataFrame}, @code{Rle}, and @code{Hits}) are implemented in the
S4Vectors package itself.")
    (license license:artistic2.0)))

(define-public r-iranges
  (package
    (name "r-iranges")
    (version "2.22.2")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "IRanges" version))
              (sha256
               (base32
                "1y24jw62806wp2afiyj1x6n00gj7d3323klqdypra9q43pg1w49d"))))
    (properties
     `((upstream-name . "IRanges")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-s4vectors" ,r-s4vectors)))
    (home-page "https://bioconductor.org/packages/IRanges")
    (synopsis "Infrastructure for manipulating intervals on sequences")
    (description
     "This package provides efficient low-level and highly reusable S4 classes
for storing ranges of integers, RLE vectors (Run-Length Encoding), and, more
generally, data that can be organized sequentially (formally defined as
@code{Vector} objects), as well as views on these @code{Vector} objects.
Efficient list-like classes are also provided for storing big collections of
instances of the basic classes.  All classes in the package use consistent
naming and share the same rich and consistent \"Vector API\" as much as
possible.")
    (license license:artistic2.0)))

(define-public r-genomeinfodbdata
  (package
    (name "r-genomeinfodbdata")
    (version "1.2.0")
    (source (origin
              (method url-fetch)
              ;; We cannot use bioconductor-uri here because this tarball is
              ;; located under "data/annotation/" instead of "bioc/".
              (uri (string-append "https://bioconductor.org/packages/release/"
                                  "data/annotation/src/contrib/GenomeInfoDbData_"
                                  version ".tar.gz"))
              (sha256
               (base32
                "0di6nlqpsyqf693k2na65ayqldih563x3zfrczpqc5q2hl5kg35c"))))
    (properties
     `((upstream-name . "GenomeInfoDbData")))
    (build-system r-build-system)
    (home-page "https://bioconductor.org/packages/GenomeInfoDbData")
    (synopsis "Species and taxonomy ID look up tables for GenomeInfoDb")
    (description "This package contains data for mapping between NCBI taxonomy
ID and species.  It is used by functions in the GenomeInfoDb package.")
    (license license:artistic2.0)))

(define-public r-genomeinfodb
  (package
    (name "r-genomeinfodb")
    (version "1.24.2")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "GenomeInfoDb" version))
              (sha256
               (base32
                "1cqs53p4m5q1dr59war72bccphy01ilw4xra24fmngrv4x32rznd"))))
    (properties
     `((upstream-name . "GenomeInfoDb")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-genomeinfodbdata" ,r-genomeinfodbdata)
       ("r-iranges" ,r-iranges)
       ("r-rcurl" ,r-rcurl)
       ("r-s4vectors" ,r-s4vectors)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/GenomeInfoDb")
    (synopsis "Utilities for manipulating chromosome identifiers")
    (description
     "This package contains data and functions that define and allow
translation between different chromosome sequence naming conventions (e.g.,
\"chr1\" versus \"1\"), including a function that attempts to place sequence
names in their natural, rather than lexicographic, order.")
    (license license:artistic2.0)))

(define-public r-edger
  (package
    (name "r-edger")
    (version "3.30.3")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "edgeR" version))
              (sha256
               (base32
                "1z9bkg08rgqn3jm2s4ndbj641w33wl8jd3j6m5if6h2nnw6011ic"))))
    (properties `((upstream-name . "edgeR")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-limma" ,r-limma)
       ("r-locfit" ,r-locfit)
       ("r-rcpp" ,r-rcpp)
       ("r-statmod" ,r-statmod))) ;for estimateDisp
    (home-page "http://bioinf.wehi.edu.au/edgeR")
    (synopsis "EdgeR does empirical analysis of digital gene expression data")
    (description "This package can do differential expression analysis of
RNA-seq expression profiles with biological replication.  It implements a range
of statistical methodology based on the negative binomial distributions,
including empirical Bayes estimation, exact tests, generalized linear models
and quasi-likelihood tests.  It be applied to differential signal analysis of
other types of genomic data that produce counts, including ChIP-seq, SAGE and
CAGE.")
    (license license:gpl2+)))

(define-public r-variantannotation
  (package
    (name "r-variantannotation")
    (version "1.34.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "VariantAnnotation" version))
              (sha256
               (base32
                "09y6ymwky839nb0y7y93w810hk9mvwqn7595q1276c28dkddiqvw"))))
    (properties
     `((upstream-name . "VariantAnnotation")))
    (inputs
     `(("zlib" ,zlib)))
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-biostrings" ,r-biostrings)
       ("r-bsgenome" ,r-bsgenome)
       ("r-dbi" ,r-dbi)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicfeatures" ,r-genomicfeatures)
       ("r-genomicranges" ,r-genomicranges)
       ("r-iranges" ,r-iranges)
       ("r-summarizedexperiment" ,r-summarizedexperiment)
       ("r-rhtslib" ,r-rhtslib)
       ("r-rsamtools" ,r-rsamtools)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-s4vectors" ,r-s4vectors)
       ("r-xvector" ,r-xvector)
       ("r-zlibbioc" ,r-zlibbioc)))
    (build-system r-build-system)
    (home-page "https://bioconductor.org/packages/VariantAnnotation")
    (synopsis "Package for annotation of genetic variants")
    (description "This R package can annotate variants, compute amino acid
coding changes and predict coding outcomes.")
    (license license:artistic2.0)))

(define-public r-limma
  (package
    (name "r-limma")
    (version "3.44.3")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "limma" version))
              (sha256
               (base32
                "09fnqxx4rzq5n447aqg2l6y0idfwgz2jxz99sifxsr2q8afzbcj6"))))
    (build-system r-build-system)
    (home-page "http://bioinf.wehi.edu.au/limma")
    (synopsis "Package for linear models for microarray and RNA-seq data")
    (description "This package can be used for the analysis of gene expression
studies, especially the use of linear models for analysing designed experiments
and the assessment of differential expression.  The analysis methods apply to
different technologies, including microarrays, RNA-seq, and quantitative PCR.")
    (license license:gpl2+)))

(define-public r-xvector
  (package
    (name "r-xvector")
    (version "0.28.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "XVector" version))
              (sha256
               (base32
                "11h1hszv4798q1gbx8r6zf8vlaqx4v9ql0lbh2xaxybp66a03pvc"))))
    (properties
     `((upstream-name . "XVector")))
    (build-system r-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'use-system-zlib
           (lambda _
             (substitute* "DESCRIPTION"
               (("zlibbioc, ") ""))
             (substitute* "NAMESPACE"
               (("import\\(zlibbioc\\)") ""))
             #t)))))
    (inputs
     `(("zlib" ,zlib)))
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-iranges" ,r-iranges)
       ("r-s4vectors" ,r-s4vectors)))
    (home-page "https://bioconductor.org/packages/XVector")
    (synopsis "Representation and manpulation of external sequences")
    (description
     "This package provides memory efficient S4 classes for storing sequences
\"externally\" (behind an R external pointer, or on disk).")
    (license license:artistic2.0)))

(define-public r-genomicranges
  (package
    (name "r-genomicranges")
    (version "1.40.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "GenomicRanges" version))
              (sha256
               (base32
                "0wn1zr2qq0rpv9z2wialgizn6xzdlcjg1w2kif67n53svz6vk2x1"))))
    (properties
     `((upstream-name . "GenomicRanges")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-iranges" ,r-iranges)
       ("r-s4vectors" ,r-s4vectors)
       ("r-xvector" ,r-xvector)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/GenomicRanges")
    (synopsis "Representation and manipulation of genomic intervals")
    (description
     "This package provides tools to efficiently represent and manipulate
genomic annotations and alignments is playing a central role when it comes to
analyzing high-throughput sequencing data (a.k.a. NGS data).  The
GenomicRanges package defines general purpose containers for storing and
manipulating genomic intervals and variables defined along a genome.")
    (license license:artistic2.0)))

(define-public r-biobase
  (package
    (name "r-biobase")
    (version "2.48.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "Biobase" version))
              (sha256
               (base32
                "13p3kgnxm7hbn8cy289kbhaiyfa6rxx7l1pbvajwqzbay3cxznqp"))))
    (properties
     `((upstream-name . "Biobase")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)))
    (home-page "https://bioconductor.org/packages/Biobase")
    (synopsis "Base functions for Bioconductor")
    (description
     "This package provides functions that are needed by many other packages
on Bioconductor or which replace R functions.")
    (license license:artistic2.0)))

(define-public r-annotationdbi
  (package
    (name "r-annotationdbi")
    (version "1.50.3")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "AnnotationDbi" version))
              (sha256
               (base32
                "0qcxfn4pvaksna0nvxr74ysn2wzaxn732pkhd6ffrj8pwhkhy5p3"))))
    (properties
     `((upstream-name . "AnnotationDbi")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-dbi" ,r-dbi)
       ("r-iranges" ,r-iranges)
       ("r-rsqlite" ,r-rsqlite)
       ("r-s4vectors" ,r-s4vectors)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/AnnotationDbi")
    (synopsis "Annotation database interface")
    (description
     "This package provides user interface and database connection code for
annotation data packages using SQLite data storage.")
    (license license:artistic2.0)))

(define-public r-biomart
  (package
    (name "r-biomart")
    (version "2.44.1")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "biomaRt" version))
              (sha256
               (base32
                "0np4nh3gj60mgb6312z7x0z9fg5bhrhw872sp3dzgmqc8q8b84iz"))))
    (properties
     `((upstream-name . "biomaRt")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-biocfilecache" ,r-biocfilecache)
       ("r-httr" ,r-httr)
       ("r-openssl" ,r-openssl)
       ("r-progress" ,r-progress)
       ("r-rappdirs" ,r-rappdirs)
       ("r-stringr" ,r-stringr)
       ("r-xml" ,r-xml)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/biomaRt")
    (synopsis "Interface to BioMart databases")
    (description
     "biomaRt provides an interface to a growing collection of databases
implementing the @url{BioMart software suite, http://www.biomart.org}.  The
package enables retrieval of large amounts of data in a uniform way without
the need to know the underlying database schemas or write complex SQL queries.
Examples of BioMart databases are Ensembl, COSMIC, Uniprot, HGNC, Gramene,
Wormbase and dbSNP mapped to Ensembl.  These major databases give biomaRt
users direct access to a diverse set of data and enable a wide range of
powerful online queries from gene annotation to database mining.")
    (license license:artistic2.0)))

(define-public r-biocparallel
  (package
    (name "r-biocparallel")
    (version "1.22.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "BiocParallel" version))
              (sha256
               (base32
                "1lsg5xm5j3ly5k60fidzhkgqc9jgscyfkqngjvd9qp6yfzvsb82g"))))
    (properties
     `((upstream-name . "BiocParallel")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-futile-logger" ,r-futile-logger)
       ("r-snow" ,r-snow)
       ("r-bh" ,r-bh)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/BiocParallel")
    (synopsis "Bioconductor facilities for parallel evaluation")
    (description
     "This package provides modified versions and novel implementation of
functions for parallel evaluation, tailored to use with Bioconductor
objects.")
    (license (list license:gpl2+ license:gpl3+))))

(define-public r-biostrings
  (package
    (name "r-biostrings")
    (version "2.56.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "Biostrings" version))
              (sha256
               (base32
                "0imhfz7dg8b3l5qzipjranqqshdsg2x6zc49drlhn8sc7j40cvi8"))))
    (properties
     `((upstream-name . "Biostrings")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-crayon" ,r-crayon)
       ("r-iranges" ,r-iranges)
       ("r-s4vectors" ,r-s4vectors)
       ("r-xvector" ,r-xvector)))
    (home-page "https://bioconductor.org/packages/Biostrings")
    (synopsis "String objects and algorithms for biological sequences")
    (description
     "This package provides memory efficient string containers, string
matching algorithms, and other utilities, for fast manipulation of large
biological sequences or sets of sequences.")
    (license license:artistic2.0)))

(define-public r-rsamtools
  (package
    (name "r-rsamtools")
    (version "2.4.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "Rsamtools" version))
              (sha256
               (base32
                "0z01z0s71f941k7sns46nyabps28c69d6jxx6sppjpc6h4vrw0vq"))))
    (properties
     `((upstream-name . "Rsamtools")))
    (build-system r-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'use-system-zlib
           (lambda _
             (substitute* "DESCRIPTION"
               (("zlibbioc, ") ""))
             (substitute* "NAMESPACE"
               (("import\\(zlibbioc\\)") ""))
             #t)))))
    (inputs
     `(("zlib" ,zlib)))
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-biocparallel" ,r-biocparallel)
       ("r-biostrings" ,r-biostrings)
       ("r-bitops" ,r-bitops)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicranges" ,r-genomicranges)
       ("r-iranges" ,r-iranges)
       ("r-rhtslib" ,r-rhtslib)
       ("r-s4vectors" ,r-s4vectors)
       ("r-xvector" ,r-xvector)))
    (home-page "https://bioconductor.org/packages/release/bioc/html/Rsamtools.html")
    (synopsis "Interface to samtools, bcftools, and tabix")
    (description
     "This package provides an interface to the @code{samtools},
@code{bcftools}, and @code{tabix} utilities for manipulating SAM (Sequence
Alignment / Map), FASTA, binary variant call (BCF) and compressed indexed
tab-delimited (tabix) files.")
    (license license:expat)))

(define-public r-delayedarray
  (package
    (name "r-delayedarray")
    (version "0.14.1")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "DelayedArray" version))
              (sha256
               (base32
                "0xi0i621hrl7k9rsc8x8nc3ib74sk8hxhbyr2lxqclw45pk95s7v"))))
    (properties
     `((upstream-name . "DelayedArray")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-s4vectors" ,r-s4vectors)
       ("r-iranges" ,r-iranges)
       ("r-matrix" ,r-matrix)
       ("r-matrixstats" ,r-matrixstats)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/DelayedArray")
    (synopsis "Delayed operations on array-like objects")
    (description
     "Wrapping an array-like object (typically an on-disk object) in a
@code{DelayedArray} object allows one to perform common array operations on it
without loading the object in memory.  In order to reduce memory usage and
optimize performance, operations on the object are either delayed or executed
using a block processing mechanism.  Note that this also works on in-memory
array-like objects like @code{DataFrame} objects (typically with Rle columns),
@code{Matrix} objects, and ordinary arrays and data frames.")
    (license license:artistic2.0)))

(define-public r-summarizedexperiment
  (package
    (name "r-summarizedexperiment")
    (version "1.18.2")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "SummarizedExperiment" version))
              (sha256
               (base32
                "1raw5ycigr6gjgzn3kx3jls5hzww10fhnwd8c17c20hmhirf13rw"))))
    (properties
     `((upstream-name . "SummarizedExperiment")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-delayedarray" ,r-delayedarray)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicranges" ,r-genomicranges)
       ("r-iranges" ,r-iranges)
       ("r-matrix" ,r-matrix)
       ("r-s4vectors" ,r-s4vectors)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/SummarizedExperiment")
    (synopsis "Container for representing genomic ranges by sample")
    (description
     "The SummarizedExperiment container contains one or more assays, each
represented by a matrix-like object of numeric or other mode.  The rows
typically represent genomic ranges of interest and the columns represent
samples.")
    (license license:artistic2.0)))

(define-public r-genomicalignments
  (package
    (name "r-genomicalignments")
    (version "1.24.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "GenomicAlignments" version))
              (sha256
               (base32
                "0v8k6d7frm5p48cmk4zik78cw9abz4inx0zhl4zrmmx31ifyvk8d"))))
    (properties
     `((upstream-name . "GenomicAlignments")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-biocparallel" ,r-biocparallel)
       ("r-biostrings" ,r-biostrings)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicranges" ,r-genomicranges)
       ("r-iranges" ,r-iranges)
       ("r-rsamtools" ,r-rsamtools)
       ("r-s4vectors" ,r-s4vectors)
       ("r-summarizedexperiment" ,r-summarizedexperiment)))
    (home-page "https://bioconductor.org/packages/GenomicAlignments")
    (synopsis "Representation and manipulation of short genomic alignments")
    (description
     "This package provides efficient containers for storing and manipulating
short genomic alignments (typically obtained by aligning short reads to a
reference genome).  This includes read counting, computing the coverage,
junction detection, and working with the nucleotide content of the
alignments.")
    (license license:artistic2.0)))

(define-public r-rtracklayer
  (package
    (name "r-rtracklayer")
    (version "1.48.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "rtracklayer" version))
              (sha256
               (base32
                "1zcgk92sidhy4y7ws9ms4nkkh2hnccfhfh53qgna0kma9jy4v5xf"))))
    (build-system r-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'use-system-zlib
           (lambda _
             (substitute* "DESCRIPTION"
               ((" zlibbioc,") ""))
             (substitute* "NAMESPACE"
               (("import\\(zlibbioc\\)") ""))
             #t)))))
    (native-inputs
     `(("pkg-config" ,pkg-config)))
    (inputs
     `(("zlib" ,zlib)))
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-biostrings" ,r-biostrings)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicalignments" ,r-genomicalignments)
       ("r-genomicranges" ,r-genomicranges)
       ("r-iranges" ,r-iranges)
       ("r-rcurl" ,r-rcurl)
       ("r-rsamtools" ,r-rsamtools)
       ("r-s4vectors" ,r-s4vectors)
       ("r-xml" ,r-xml)
       ("r-xvector" ,r-xvector)))
    (home-page "https://bioconductor.org/packages/rtracklayer")
    (synopsis "R interface to genome browsers and their annotation tracks")
    (description
     "rtracklayer is an extensible framework for interacting with multiple
genome browsers (currently UCSC built-in) and manipulating annotation tracks
in various formats (currently GFF, BED, bedGraph, BED15, WIG, BigWig and 2bit
built-in).  The user may export/import tracks to/from the supported browsers,
as well as query and modify the browser state, such as the current viewport.")
    (license license:artistic2.0)))

(define-public r-genomicfeatures
  (package
    (name "r-genomicfeatures")
    (version "1.40.1")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "GenomicFeatures" version))
              (sha256
               (base32
                "0zm7q957g952qyfqmvf27sldsnq6sqd0wlgnqzvmxayg9pxh0l0z"))))
    (properties
     `((upstream-name . "GenomicFeatures")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-biomart" ,r-biomart)
       ("r-biostrings" ,r-biostrings)
       ("r-dbi" ,r-dbi)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicranges" ,r-genomicranges)
       ("r-iranges" ,r-iranges)
       ("r-rcurl" ,r-rcurl)
       ("r-rsqlite" ,r-rsqlite)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-s4vectors" ,r-s4vectors)
       ("r-xvector" ,r-xvector)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/GenomicFeatures")
    (synopsis "Tools for working with transcript centric annotations")
    (description
     "This package provides a set of tools and methods for making and
manipulating transcript centric annotations.  With these tools the user can
easily download the genomic locations of the transcripts, exons and cds of a
given organism, from either the UCSC Genome Browser or a BioMart
database (more sources will be supported in the future).  This information is
then stored in a local database that keeps track of the relationship between
transcripts, exons, cds and genes.  Flexible methods are provided for
extracting the desired features in a convenient format.")
    (license license:artistic2.0)))

(define-public r-go-db
  (package
    (name "r-go-db")
    (version "3.7.0")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://www.bioconductor.org/packages/"
                                  "release/data/annotation/src/contrib/GO.db_"
                                  version ".tar.gz"))
              (sha256
               (base32
                "0i3wcf5h3n0dawzc1hy0kv74f06j80c47n4p3g3fmrcxlhi3jpa5"))))
    (properties
     `((upstream-name . "GO.db")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)))
    (home-page "https://bioconductor.org/packages/GO.db")
    (synopsis "Annotation maps describing the entire Gene Ontology")
    (description
     "The purpose of this GO.db annotation package is to provide detailed
information about the latest version of the Gene Ontologies.")
    (license license:artistic2.0)))

(define-public r-topgo
  (package
    (name "r-topgo")
    (version "2.40.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "topGO" version))
              (sha256
               (base32
                "13rhbvn27sj75fklf1cnjaazacx8yyjlhqlnbp5zk157q6y5cwdr"))))
    (properties
     `((upstream-name . "topGO")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-dbi" ,r-dbi)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-go-db" ,r-go-db)
       ("r-graph" ,r-graph)
       ("r-lattice" ,r-lattice)
       ("r-matrixstats" ,r-matrixstats)
       ("r-sparsem" ,r-sparsem)))
    (home-page "https://bioconductor.org/packages/topGO")
    (synopsis "Enrichment analysis for gene ontology")
    (description
     "The topGO package provides tools for testing @dfn{gene ontology} (GO)
terms while accounting for the topology of the GO graph.  Different test
statistics and different methods for eliminating local similarities and
dependencies between GO terms can be implemented and applied.")
    ;; Any version of the LGPL applies.
    (license license:lgpl2.1+)))

(define-public r-bsgenome
  (package
    (name "r-bsgenome")
    (version "1.56.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "BSgenome" version))
              (sha256
               (base32
                "1jw8r1qm9fpg2s1cw2y4np243jjxm65j2xdy2785h8fc1b02msf6"))))
    (properties
     `((upstream-name . "BSgenome")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-biostrings" ,r-biostrings)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicranges" ,r-genomicranges)
       ("r-iranges" ,r-iranges)
       ("r-matrixstats" ,r-matrixstats)
       ("r-rsamtools" ,r-rsamtools)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-s4vectors" ,r-s4vectors)
       ("r-xvector" ,r-xvector)))
    (home-page "https://bioconductor.org/packages/BSgenome")
    (synopsis "Infrastructure for Biostrings-based genome data packages")
    (description
     "This package provides infrastructure shared by all Biostrings-based
genome data packages and support for efficient SNP representation.")
    (license license:artistic2.0)))

(define-public r-impute
  (package
    (name "r-impute")
    (version "1.62.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "impute" version))
              (sha256
               (base32
                "161p6l1cp3wwdynkxwvg0yhrh6yv20brdlplw5w5mavn4hf1nm0h"))))
    (native-inputs
     `(("gfortran" ,gfortran)))
    (build-system r-build-system)
    (home-page "https://bioconductor.org/packages/impute")
    (synopsis "Imputation for microarray data")
    (description
     "This package provides a function to impute missing gene expression
microarray data, using nearest neighbor averaging.")
    (license license:gpl2+)))

(define-public r-seqpattern
  (package
    (name "r-seqpattern")
    (version "1.20.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "seqPattern" version))
              (sha256
               (base32
                "0f1yvx2ri1557rzjx08q5bgml1cvkm8hjl8xn1qi4rjs64sy6mci"))))
    (properties
     `((upstream-name . "seqPattern")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biostrings" ,r-biostrings)
       ("r-genomicranges" ,r-genomicranges)
       ("r-iranges" ,r-iranges)
       ("r-kernsmooth" ,r-kernsmooth)
       ("r-plotrix" ,r-plotrix)))
    (home-page "https://bioconductor.org/packages/seqPattern")
    (synopsis "Visualising oligonucleotide patterns and motif occurrences")
    (description
     "This package provides tools to visualize oligonucleotide patterns and
sequence motif occurrences across a large set of sequences centred at a common
reference point and sorted by a user defined feature.")
    (license license:gpl3+)))

(define-public r-genomation
  (package
    (name "r-genomation")
    (version "1.20.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "genomation" version))
              (sha256
               (base32
                "1cy8kqwddiha5jy6nda1al956i4wncbgjkrxwijdb08cmka2sfwh"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biostrings" ,r-biostrings)
       ("r-bsgenome" ,r-bsgenome)
       ("r-data-table" ,r-data-table)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicalignments" ,r-genomicalignments)
       ("r-genomicranges" ,r-genomicranges)
       ("r-ggplot2" ,r-ggplot2)
       ("r-gridbase" ,r-gridbase)
       ("r-impute" ,r-impute)
       ("r-iranges" ,r-iranges)
       ("r-matrixstats" ,r-matrixstats)
       ("r-plotrix" ,r-plotrix)
       ("r-plyr" ,r-plyr)
       ("r-rcpp" ,r-rcpp)
       ("r-readr" ,r-readr)
       ("r-reshape2" ,r-reshape2)
       ("r-rsamtools" ,r-rsamtools)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-runit" ,r-runit)
       ("r-s4vectors" ,r-s4vectors)
       ("r-seqpattern" ,r-seqpattern)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioinformatics.mdc-berlin.de/genomation/")
    (synopsis "Summary, annotation and visualization of genomic data")
    (description
     "This package provides a package for summary and annotation of genomic
intervals.  Users can visualize and quantify genomic intervals over
pre-defined functional regions, such as promoters, exons, introns, etc.  The
genomic intervals represent regions with a defined chromosome position, which
may be associated with a score, such as aligned reads from HT-seq experiments,
TF binding sites, methylation scores, etc.  The package can use any tabular
genomic feature data as long as it has minimal information on the locations of
genomic intervals.  In addition, it can use BAM or BigWig files as input.")
    (license license:artistic2.0)))

(define-public r-genomationdata
  (package
    (name "r-genomationdata")
    (version "1.14.0")
    (source (origin
              (method url-fetch)
              ;; We cannot use bioconductor-uri here because this tarball is
              ;; located under "data/annotation/" instead of "bioc/".
              (uri (string-append "https://bioconductor.org/packages/"
                                  "release/data/experiment/src/contrib/"
                                  "genomationData_" version ".tar.gz"))
              (sha256
               (base32
                "10xyb8akjrhmak2i0mnv1agny2ipy364q9nlibyplpzc7vdb6bw7"))))
    (build-system r-build-system)
    ;; As this package provides little more than large data files, it doesn't
    ;; make sense to build substitutes.
    (arguments `(#:substitutable? #f))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioinformatics.mdc-berlin.de/genomation/")
    (synopsis "Experimental data for use with the genomation package")
    (description
     "This package contains experimental genetic data for use with the
genomation package.  Included are Chip Seq, Methylation and Cage data,
downloaded from Encode.")
    (license license:gpl3+)))

(define-public r-seqlogo
  (package
    (name "r-seqlogo")
    (version "1.54.3")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "seqLogo" version))
       (sha256
        (base32
         "09kkxir305fv8z2yyihvspkrzclxbw1cx5mvhfkrhl10rap6662j"))))
    (properties `((upstream-name . "seqLogo")))
    (build-system r-build-system)
    (home-page "https://bioconductor.org/packages/seqLogo")
    (synopsis "Sequence logos for DNA sequence alignments")
    (description
     "seqLogo takes the position weight matrix of a DNA sequence motif and
plots the corresponding sequence logo as introduced by Schneider and
Stephens (1990).")
    (license license:lgpl2.0+)))

(define-public r-motifrg
  (package
    (name "r-motifrg")
    (version "1.31.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "motifRG" version))
       (sha256
        (base32
         "1ml6zyzlk8yjbnfhga2qnw8nl43rankvka0kc1yljxr2b66aqbhn"))))
    (properties `((upstream-name . "motifRG")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biostrings" ,r-biostrings)
       ("r-bsgenome" ,r-bsgenome)
       ("r-bsgenome-hsapiens-ucsc-hg19" ,r-bsgenome-hsapiens-ucsc-hg19)
       ("r-iranges" ,r-iranges)
       ("r-seqlogo" ,r-seqlogo)
       ("r-xvector" ,r-xvector)))
    (home-page "https://bioconductor.org/packages/motifRG")
    (synopsis "Discover motifs in high throughput sequencing data")
    (description
     "This package provides tools for discriminative motif discovery in high
throughput genetic sequencing data sets using regression methods.")
    (license license:artistic2.0)))

(define-public r-zlibbioc
  (package
    (name "r-zlibbioc")
    (version "1.34.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "zlibbioc" version))
              (sha256
               (base32
                "0j1l052jb2cwc1nifxzwknc9csagf4f2d092zs0i95dz0rma89l0"))))
    (properties
     `((upstream-name . "zlibbioc")))
    (build-system r-build-system)
    (home-page "https://bioconductor.org/packages/zlibbioc")
    (synopsis "Provider for zlib-1.2.5 to R packages")
    (description "This package uses the source code of zlib-1.2.5 to create
libraries for systems that do not have these available via other means.")
    (license license:artistic2.0)))

(define-public r-r4rna
  (package
    (name "r-r4rna")
    (version "0.1.4")
    (source
     (origin
       (method url-fetch)
       (uri (string-append "http://www.e-rna.org/r-chie/files/R4RNA_"
                           version ".tar.gz"))
       (sha256
        (base32
         "1p0i78wh76jfgmn9jphbwwaz6yy6pipzfg08xs54cxavxg2j81p5"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-optparse" ,r-optparse)
       ("r-rcolorbrewer" ,r-rcolorbrewer)))
    (home-page "https://www.e-rna.org/r-chie/index.cgi")
    (synopsis "Analysis framework for RNA secondary structure")
    (description
     "The R4RNA package aims to be a general framework for the analysis of RNA
secondary structure and comparative analysis in R.")
    (license license:gpl3+)))

(define-public r-rhtslib
  (package
    (name "r-rhtslib")
    (version "1.20.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "Rhtslib" version))
       (sha256
        (base32
         "186r7icrkzrni1c4n33ip7dlsfgys7hnqf0simvxrpl3yhh3ygdi"))))
    (properties `((upstream-name . "Rhtslib")))
    (build-system r-build-system)
    ;; Without this a temporary directory ends up in the Rhtslib.so binary,
    ;; which makes R abort the build.
    (arguments '(#:configure-flags '("--no-staged-install")))
    (propagated-inputs
     `(("curl" ,curl)
       ("r-zlibbioc" ,r-zlibbioc)))
    (inputs
     `(("zlib" ,zlib)))
    (native-inputs
     `(("pkg-config" ,pkg-config)
       ("r-knitr" ,r-knitr)))
    (home-page "https://github.com/nhayden/Rhtslib")
    (synopsis "High-throughput sequencing library as an R package")
    (description
     "This package provides the HTSlib C library for high-throughput
nucleotide sequence analysis.  The package is primarily useful to developers
of other R packages who wish to make use of HTSlib.")
    (license license:lgpl2.0+)))

(define-public r-bamsignals
  (package
    (name "r-bamsignals")
    (version "1.20.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "bamsignals" version))
       (sha256
        (base32
         "0p858xxfv79yc8b3lq58zl9f00irvbn3czsd8wdi5040xg42m402"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-genomicranges" ,r-genomicranges)
       ("r-iranges" ,r-iranges)
       ("r-rcpp" ,r-rcpp)
       ("r-rhtslib" ,r-rhtslib)
       ("r-zlibbioc" ,r-zlibbioc)))
    (inputs
     `(("zlib" ,zlib)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/bamsignals")
    (synopsis "Extract read count signals from bam files")
    (description
     "This package efficiently obtains count vectors from indexed bam
files.  It counts the number of nucleotide sequence reads in given genomic
ranges and it computes reads profiles and coverage profiles.  It also handles
paired-end data.")
    (license license:gpl2+)))

(define-public r-rcas
  (package
    (name "r-rcas")
    (version "1.14.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "RCAS" version))
              (sha256
               (base32
                "0f812pgv3ys1zv4n9sqkgm01hj4cdd0i0h85dqbhkwd94zl6cavl"))))
    (properties `((upstream-name . "RCAS")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-biostrings" ,r-biostrings)
       ("r-bsgenome" ,r-bsgenome)
       ("r-bsgenome-hsapiens-ucsc-hg19" ,r-bsgenome-hsapiens-ucsc-hg19)
       ("r-cowplot" ,r-cowplot)
       ("r-data-table" ,r-data-table)
       ("r-dt" ,r-dt)
       ("r-genomation" ,r-genomation)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicfeatures" ,r-genomicfeatures)
       ("r-genomicranges" ,r-genomicranges)
       ("r-ggplot2" ,r-ggplot2)
       ("r-ggseqlogo" ,r-ggseqlogo)
       ("r-gprofiler2" ,r-gprofiler2)
       ("r-iranges" ,r-iranges)
       ("r-pbapply" ,r-pbapply)
       ("r-pheatmap" ,r-pheatmap)
       ("r-plotly" ,r-plotly)
       ("r-plotrix" ,r-plotrix)
       ("r-proxy" ,r-proxy)
       ("r-ranger" ,r-ranger)
       ("r-rsqlite" ,r-rsqlite)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-rmarkdown" ,r-rmarkdown)
       ("r-s4vectors" ,r-s4vectors)
       ("pandoc" ,pandoc)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (synopsis "RNA-centric annotation system")
    (description
     "RCAS aims to be a standalone RNA-centric annotation system that provides
intuitive reports and publication-ready graphics.  This package provides the R
library implementing most of the pipeline's features.")
    (home-page "https://github.com/BIMSBbioinfo/RCAS")
    (license license:artistic2.0)))

(define-public rcas-web
  (package
    (name "rcas-web")
    (version "0.1.0")
    (source
     (origin
       (method url-fetch)
       (uri (string-append "https://github.com/BIMSBbioinfo/rcas-web/"
                           "releases/download/v" version
                           "/rcas-web-" version ".tar.gz"))
       (sha256
        (base32
         "0wq951aj45gqki1bickg876i993lmawkp8x24agg264br5x716db"))))
    (build-system gnu-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-before 'configure 'find-RCAS
           ;; The configure script can't find non-1.3.x versions of RCAS because
           ;; its R expression ‘1.10.1 >= 1.3.4’ evaluates to false.
           (lambda _
             (substitute* "configure"
               (("1\\.3\\.4") "0.0.0"))
             #t))
         (add-after 'install 'wrap-executable
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (json   (assoc-ref inputs "guile-json"))
                    (redis  (assoc-ref inputs "guile-redis"))
                    (path   (string-append
                             json  "/share/guile/site/2.2:"
                             redis "/share/guile/site/2.2")))
               (wrap-program (string-append out "/bin/rcas-web")
                 `("GUILE_LOAD_PATH" ":" = (,path))
                 `("GUILE_LOAD_COMPILED_PATH" ":" = (,path))
                 `("R_LIBS_SITE" ":" = (,(getenv "R_LIBS_SITE")))))
             #t)))))
    (inputs
     `(("r-minimal" ,r-minimal)
       ("r-rcas" ,r-rcas)
       ("guile" ,guile-2.2)
       ("guile-json" ,guile-json-1)
       ("guile-redis" ,guile2.2-redis)))
    (native-inputs
     `(("pkg-config" ,pkg-config)))
    (home-page "https://github.com/BIMSBbioinfo/rcas-web")
    (synopsis "Web interface for RNA-centric annotation system (RCAS)")
    (description "This package provides a simple web interface for the
@dfn{RNA-centric annotation system} (RCAS).")
    (license license:agpl3+)))

(define-public r-mutationalpatterns
  (package
    (name "r-mutationalpatterns")
    (version "2.0.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "MutationalPatterns" version))
       (sha256
        (base32
         "02lyjiabyhmifycksvpcx29a0pb7z9xjw0hgg8n0sd0dy3afqhcm"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-biostrings" ,r-biostrings)
       ;; These two packages are suggested packages
       ("r-bsgenome-hsapiens-1000g" ,r-bsgenome-hsapiens-1000genomes-hs37d5)
       ("r-bsgenome-hsapiens-ucsc-hg19" ,r-bsgenome-hsapiens-ucsc-hg19)
       ("r-genomicranges" ,r-genomicranges)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-ggplot2" ,r-ggplot2)
       ("r-iranges" ,r-iranges)
       ("r-nmf" ,r-nmf)
       ("r-plyr" ,r-plyr)
       ("r-pracma" ,r-pracma)
       ("r-reshape2" ,r-reshape2)
       ("r-cowplot" ,r-cowplot)
       ("r-ggdendro" ,r-ggdendro)
       ("r-s4vectors" ,r-s4vectors)
       ("r-summarizedexperiment" ,r-summarizedexperiment)
       ("r-variantannotation" ,r-variantannotation)))
    (home-page "https://bioconductor.org/packages/MutationalPatterns/")
    (synopsis "Extract and visualize mutational patterns in genomic data")
    (description "This package provides an extensive toolset for the
characterization and visualization of a wide range of mutational patterns
in SNV base substitution data.")
    (license license:expat)))

(define-public r-chipkernels
  (let ((commit "c9cfcacb626b1221094fb3490ea7bac0fd625372")
        (revision "1"))
    (package
      (name "r-chipkernels")
      (version (string-append "1.1-" revision "." (string-take commit 9)))
      (source
       (origin
         (method git-fetch)
         (uri (git-reference
               (url "https://github.com/ManuSetty/ChIPKernels")
               (commit commit)))
         (file-name (string-append name "-" version))
         (sha256
          (base32
           "14bj5qhjm1hsm9ay561nfbqi9wxsa7y487df2idsaaf6z10nw4v0"))))
      (build-system r-build-system)
      (propagated-inputs
       `(("r-iranges" ,r-iranges)
         ("r-xvector" ,r-xvector)
         ("r-biostrings" ,r-biostrings)
         ("r-bsgenome" ,r-bsgenome)
         ("r-gtools" ,r-gtools)
         ("r-genomicranges" ,r-genomicranges)
         ("r-sfsmisc" ,r-sfsmisc)
         ("r-kernlab" ,r-kernlab)
         ("r-s4vectors" ,r-s4vectors)
         ("r-biocgenerics" ,r-biocgenerics)))
      (home-page "https://github.com/ManuSetty/ChIPKernels")
      (synopsis "Build string kernels for DNA Sequence analysis")
      (description "ChIPKernels is an R package for building different string
kernels used for DNA Sequence analysis.  A dictionary of the desired kernel
must be built and this dictionary can be used for determining kernels for DNA
Sequences.")
      (license license:gpl2+))))

(define-public r-seqgl
  (package
    (name "r-seqgl")
    (version "1.1.4")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/ManuSetty/SeqGL")
             (commit version)))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "1r6ywvhxl3ffv48lgj7sbd582mcc6dha3ksgc2qjlvjrnkbj3799"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biostrings" ,r-biostrings)
       ("r-chipkernels" ,r-chipkernels)
       ("r-genomicranges" ,r-genomicranges)
       ("r-spams" ,r-spams)
       ("r-wgcna" ,r-wgcna)
       ("r-fastcluster" ,r-fastcluster)))
    (home-page "https://github.com/ManuSetty/SeqGL")
    (synopsis "Group lasso for Dnase/ChIP-seq data")
    (description "SeqGL is a group lasso based algorithm to extract
transcription factor sequence signals from ChIP, DNase and ATAC-seq profiles.
This package presents a method which uses group lasso to discriminate between
bound and non bound genomic regions to accurately identify transcription
factors bound at the specific regions.")
    (license license:gpl2+)))

(define-public r-tximport
  (package
    (name "r-tximport")
    (version "1.16.1")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "tximport" version))
              (sha256
               (base32
                "1x9959lkjl2h869rgd1b30q1idxzjkr1fyqbpndqk3kbi4q2gr40"))))
    (build-system r-build-system)
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/tximport")
    (synopsis "Import and summarize transcript-level estimates for gene-level analysis")
    (description
     "This package provides tools to import transcript-level abundance,
estimated counts and transcript lengths, and to summarize them into matrices
for use with downstream gene-level analysis packages.  Average transcript
length, weighted by sample-specific transcript abundance estimates, is
provided as a matrix which can be used as an offset for different expression
of gene-level counts.")
    (license license:gpl2+)))

(define-public r-rhdf5
  (package
    (name "r-rhdf5")
    (version "2.32.3")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "rhdf5" version))
              (sha256
               (base32
                "0fnx60dpf6s2qwq48lg98g18g8k7wgm66pw0kamlbb7l2hnwycvf"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-rhdf5lib" ,r-rhdf5lib)))
    (inputs
     `(("zlib" ,zlib)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/rhdf5")
    (synopsis "HDF5 interface to R")
    (description
     "This R/Bioconductor package provides an interface between HDF5 and R.
HDF5's main features are the ability to store and access very large and/or
complex datasets and a wide variety of metadata on mass storage (disk) through
a completely portable file format.  The rhdf5 package is thus suited for the
exchange of large and/or complex datasets between R and other software
package, and for letting R applications work on datasets that are larger than
the available RAM.")
    (license license:artistic2.0)))

(define-public r-annotationfilter
  (package
    (name "r-annotationfilter")
    (version "1.12.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "AnnotationFilter" version))
              (sha256
               (base32
                "18kh1xrhpwb48s1qj4f1v8af3jmw49pnbp5afi2myn9894hxg0cs"))))
    (properties
     `((upstream-name . "AnnotationFilter")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-genomicranges" ,r-genomicranges)
       ("r-lazyeval" ,r-lazyeval)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://github.com/Bioconductor/AnnotationFilter")
    (synopsis "Facilities for filtering Bioconductor annotation resources")
    (description
     "This package provides classes and other infrastructure to implement
filters for manipulating Bioconductor annotation resources.  The filters are
used by @code{ensembldb}, @code{Organism.dplyr}, and other packages.")
    (license license:artistic2.0)))

(define-public emboss
  (package
    (name "emboss")
    (version "6.5.7")
    (source (origin
              (method url-fetch)
              (uri (string-append "ftp://emboss.open-bio.org/pub/EMBOSS/old/"
                                  (version-major+minor version) ".0/"
                                  "EMBOSS-" version ".tar.gz"))
              (sha256
               (base32
                "0vsmz96gc411yj2iyzdrsmg4l2n1nhgmp7vrgzlxx3xixv9xbf0q"))))
    (build-system gnu-build-system)
    (arguments
     `(#:configure-flags
       (list (string-append "--with-hpdf="
                            (assoc-ref %build-inputs "libharu")))
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'fix-checks
           (lambda _
             ;; The PNGDRIVER tests check for the presence of libgd, libpng
             ;; and zlib, but assume that they are all found at the same
             ;; prefix.
             (substitute* "configure.in"
               (("CHECK_PNGDRIVER")
                "LIBS=\"$LIBS -lgd -lpng -lz -lm\"
AC_DEFINE([PLD_png], [1], [Define to 1 if PNG support is available])
AM_CONDITIONAL(AMPNG, true)"))
             #t))
         (add-after 'fix-checks 'disable-update-check
           (lambda _
             ;; At build time there is no connection to the Internet, so
             ;; looking for updates will not work.
             (substitute* "Makefile.am"
               (("\\$\\(bindir\\)/embossupdate") ""))
             #t))
         (add-after 'disable-update-check 'autogen
           (lambda _ (invoke "autoreconf" "-vif") #t)))))
    (inputs
     `(("perl" ,perl)
       ("libpng" ,libpng)
       ("gd" ,gd)
       ("libx11" ,libx11)
       ("libharu" ,libharu)
       ("zlib" ,zlib)))
    (native-inputs
     `(("autoconf" ,autoconf)
       ("automake" ,automake)
       ("libtool" ,libtool)
       ("pkg-config" ,pkg-config)))
    (home-page "http://emboss.sourceforge.net")
    (synopsis "Molecular biology analysis suite")
    (description "EMBOSS is the \"European Molecular Biology Open Software
Suite\".  EMBOSS is an analysis package specially developed for the needs of
the molecular biology (e.g. EMBnet) user community.  The software
automatically copes with data in a variety of formats and even allows
transparent retrieval of sequence data from the web.  It also provides a
number of libraries for the development of software in the field of molecular
biology.  EMBOSS also integrates a range of currently available packages and
tools for sequence analysis into a seamless whole.")
    (license license:gpl2+)))

(define-public bits
  (let ((revision "1")
        (commit "3cc4567896d9d6442923da944beb704750a08d2d"))
    (package
      (name "bits")
      ;; The version is 2.13.0 even though no release archives have been
      ;; published as yet.
      (version (string-append "2.13.0-" revision "." (string-take commit 9)))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "https://github.com/arq5x/bits")
                      (commit commit)))
                (file-name (string-append name "-" version "-checkout"))
                (sha256
                 (base32
                  "17n2kffk4kmhivd8c98g2vr6y1s23vbg4sxlxs689wni66797hbs"))))
      (build-system gnu-build-system)
      (arguments
       `(#:tests? #f ;no tests included
         #:phases
         (modify-phases %standard-phases
           (delete 'configure)
           (add-after 'unpack 'remove-cuda
             (lambda _
               (substitute* "Makefile"
                 ((".*_cuda") "")
                 (("(bits_test_intersections) \\\\" _ match) match))
               #t))
           (replace 'install
             (lambda* (#:key outputs #:allow-other-keys)
               (copy-recursively
                "bin" (string-append (assoc-ref outputs "out") "/bin"))
               #t)))))
      (inputs
       `(("gsl" ,gsl)
         ("zlib" ,zlib)))
      (home-page "https://github.com/arq5x/bits")
      (synopsis "Implementation of binary interval search algorithm")
      (description "This package provides an implementation of the
BITS (Binary Interval Search) algorithm, an approach to interval set
intersection.  It is especially suited for the comparison of diverse genomic
datasets and the exploration of large datasets of genome
intervals (e.g. genes, sequence alignments).")
      (license license:gpl2))))

(define-public piranha
  ;; There is no release tarball for the latest version.  The latest commit is
  ;; older than one year at the time of this writing.
  (let ((revision "1")
        (commit   "0466d364b71117d01e4471b74c514436cc281233"))
    (package
      (name "piranha")
      (version (string-append "1.2.1-" revision "." (string-take commit 9)))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "https://github.com/smithlabcode/piranha")
                      (commit commit)))
                (file-name (git-file-name name version))
                (sha256
                 (base32
                  "117dc0zf20c61jam69sk4abl57ah6yi6i7qra7d7y5zrbgk12q5n"))))
      (build-system gnu-build-system)
      (arguments
       `(#:test-target "test"
         #:phases
         (modify-phases %standard-phases
           (add-after 'unpack 'copy-smithlab-cpp
             (lambda* (#:key inputs #:allow-other-keys)
               (for-each (lambda (file)
                           (install-file file "./src/smithlab_cpp/"))
                         (find-files (assoc-ref inputs "smithlab-cpp")))
               #t))
           (add-after 'install 'install-to-store
             (lambda* (#:key outputs #:allow-other-keys)
               (let* ((out (assoc-ref outputs "out"))
                      (bin (string-append out "/bin")))
                 (for-each (lambda (file)
                             (install-file file bin))
                           (find-files "bin" ".*")))
               #t)))
         #:configure-flags
         (list (string-append "--with-bam_tools_headers="
                              (assoc-ref %build-inputs "bamtools") "/include/bamtools")
               (string-append "--with-bam_tools_library="
                              (assoc-ref %build-inputs "bamtools") "/lib/bamtools"))))
      (inputs
       `(("bamtools" ,bamtools)
         ("samtools" ,samtools-0.1)
         ("gsl" ,gsl)
         ("smithlab-cpp"
          ,(let ((commit "3723e2db438c51501d0423429ff396c3035ba46a"))
             (origin
               (method git-fetch)
               (uri (git-reference
                     (url "https://github.com/smithlabcode/smithlab_cpp")
                     (commit commit)))
               (file-name (string-append "smithlab_cpp-" commit "-checkout"))
               (sha256
                (base32
                 "0l4gvbwslw5ngziskja41c00x1r06l3yidv7y0xw9djibhykzy0g")))))))
      (native-inputs
       `(("python" ,python-2)))
      (home-page "https://github.com/smithlabcode/piranha")
      (synopsis "Peak-caller for CLIP-seq and RIP-seq data")
      (description
       "Piranha is a peak-caller for genomic data produced by CLIP-seq and
RIP-seq experiments.  It takes input in BED or BAM format and identifies
regions of statistically significant read enrichment.  Additional covariates
may optionally be provided to further inform the peak-calling process.")
      (license license:gpl3+))))

(define-public pepr
  (package
    (name "pepr")
    (version "1.0.9")
    (source (origin
              (method url-fetch)
              (uri (pypi-uri "PePr" version))
              (sha256
               (base32
                "0qxjfdpl1b1y53nccws2d85f6k74zwmx8y8sd9rszcqhfayx6gdx"))))
    (build-system python-build-system)
    (arguments
     `(#:python ,python-2 ; python2 only
       #:tests? #f)) ; no tests included
    (propagated-inputs
     `(("python2-numpy" ,python2-numpy)
       ("python2-scipy" ,python2-scipy)
       ("python2-pysam" ,python2-pysam)))
    (home-page "https://github.com/shawnzhangyx/PePr")
    (synopsis "Peak-calling and prioritization pipeline for ChIP-Seq data")
    (description
     "PePr is a ChIP-Seq peak calling or differential binding analysis tool
that is primarily designed for data with biological replicates.  It uses a
negative binomial distribution to model the read counts among the samples in
the same group, and look for consistent differences between ChIP and control
group or two ChIP groups run under different conditions.")
    (license license:gpl3+)))

(define-public filevercmp
  (let ((commit "1a9b779b93d0b244040274794d402106907b71b7"))
    (package
      (name "filevercmp")
      (version (string-append "0-1." (string-take commit 7)))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "https://github.com/ekg/filevercmp")
                      (commit commit)))
                (file-name (git-file-name name commit))
                (sha256
                 (base32
                  "1j9vxsy0y050v59h0q1d6501fcw1kjvj0d18l1xk2zyg0jzj247c"))))
      (build-system gnu-build-system)
      (arguments
       `(#:tests? #f ; There are no tests to run.
         #:phases
         (modify-phases %standard-phases
           (delete 'configure) ; There is no configure phase.
           (replace 'install
             (lambda* (#:key outputs #:allow-other-keys)
               (let ((bin (string-append (assoc-ref outputs "out") "/bin")))
                 (install-file "filevercmp" bin)
                 #t))))))
      (home-page "https://github.com/ekg/filevercmp")
      (synopsis "This program compares version strings")
      (description "This program compares version strings.  It intends to be a
replacement for strverscmp.")
      (license license:gpl3+))))

(define-public multiqc
  (package
    (name "multiqc")
    (version "1.5")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "multiqc" version))
       (sha256
        (base32
         "02iihfl0w0hpnr4pa0sbd1y9qxrg3ycyhjp5lidkcrqh1lmzs3zy"))))
    (build-system python-build-system)
    (propagated-inputs
     `(("python-jinja2" ,python-jinja2)
       ("python-simplejson" ,python-simplejson)
       ("python-pyyaml" ,python-pyyaml)
       ("python-click" ,python-click)
       ("python-spectra" ,python-spectra)
       ("python-requests" ,python-requests)
       ("python-markdown" ,python-markdown)
       ("python-lzstring" ,python-lzstring)
       ("python-matplotlib" ,python-matplotlib)
       ("python-numpy" ,python-numpy)
       ;; MultQC checks for the presence of nose at runtime.
       ("python-nose" ,python-nose)))
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'relax-requirements
           (lambda _
             (substitute* "setup.py"
               ;; MultiQC 1.5 ‘requires’ a version of python-matplotlib older
               ;; than the one in Guix, but should work fine with 2.2.2.
               ;; See <https://github.com/ewels/MultiQC/issues/725> and
               ;; <https://github.com/ewels/MultiQC/issues/732> for details.
               (("['\"]matplotlib.*?['\"]")
                "'matplotlib'"))
             #t)))))
    (home-page "https://multiqc.info")
    (synopsis "Aggregate bioinformatics analysis reports")
    (description
     "MultiQC is a tool to aggregate bioinformatics results across many
samples into a single report.  It contains modules for a large number of
common bioinformatics tools.")
    (license license:gpl3+)))

(define-public variant-tools
  (package
    (name "variant-tools")
    (version "3.1.2")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/vatlab/varianttools")
             ;; There is no tag corresponding to version 3.1.2
             (commit "813ae4a90d25b69abc8a40f4f70441fe09015249")))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "12ibdmksj7icyqhks4xyvd61bygk4pjmxn618kp6vgk1af01y34g"))))
    (build-system python-build-system)
    (inputs
     `(("boost" ,boost)
       ("c-blosc" ,c-blosc)
       ("gsl" ,gsl)
       ("hdf5" ,hdf5)
       ("hdf5-blosc" ,hdf5-blosc)
       ("python-cython" ,python-cython)
       ("zlib" ,zlib)))
    (propagated-inputs
     `(("python-numpy" ,python-numpy)
       ("python-pycurl" ,python-pycurl)
       ("python-pyzmq" ,python-pyzmq)
       ("python-scipy" ,python-scipy)
       ("python-tables" ,python-tables)))
    (home-page "https://vatlab.github.io/vat-docs/")
    (synopsis "Analyze genetic variants from Next-Gen sequencing studies")
    (description
     "Variant tools is a tool for the manipulation, annotation,
selection, simulation, and analysis of variants in the context of next-gen
sequencing analysis.  Unlike some other tools used for next-gen sequencing
analysis, variant tools is project based and provides a whole set of tools to
manipulate and analyze genetic variants.")
    (license license:gpl3+)))

(define-public r-chipseq
  (package
    (name "r-chipseq")
    (version "1.38.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "chipseq" version))
       (sha256
        (base32
         "0lh859s0aq73vac1phcgagf6n000qgq2xsk0bmfr61n5swifml2a"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-genomicranges" ,r-genomicranges)
       ("r-iranges" ,r-iranges)
       ("r-lattice" ,r-lattice)
       ("r-s4vectors" ,r-s4vectors)
       ("r-shortread" ,r-shortread)))
    (home-page "https://bioconductor.org/packages/chipseq")
    (synopsis "Package for analyzing ChIPseq data")
    (description
     "This package provides tools for processing short read data from ChIPseq
experiments.")
    (license license:artistic2.0)))

(define-public r-copyhelper
  (package
    (name "r-copyhelper")
    (version "1.6.0")
    (source
     (origin
       (method url-fetch)
       (uri (string-append "https://bioconductor.org/packages/release/"
                           "data/experiment/src/contrib/CopyhelpeR_"
                           version ".tar.gz"))
       (sha256
        (base32
         "0x7cyynjmxls9as2gg0iyp9x5fpalxmdjq914ss7i84i9zyk5bhq"))))
    (properties `((upstream-name . "CopyhelpeR")))
    (build-system r-build-system)
    (home-page "https://bioconductor.org/packages/CopyhelpeR/")
    (synopsis "Helper files for CopywriteR")
    (description
     "This package contains the helper files that are required to run the
Bioconductor package CopywriteR.  It contains pre-assembled 1kb bin GC-content
and mappability files for the reference genomes hg18, hg19, hg38, mm9 and
mm10.  In addition, it contains a blacklist filter to remove regions that
display copy number variation.  Files are stored as GRanges objects from the
GenomicRanges Bioconductor package.")
    (license license:gpl2)))

(define-public r-copywriter
  (package
    (name "r-copywriter")
    (version "2.20.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "CopywriteR" version))
       (sha256
        (base32
         "0c36wpv0rygkbqpf3dwh5xmc3lr7p8lrdzsq2fbbpw04skl6i7m2"))))
    (properties `((upstream-name . "CopywriteR")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocparallel" ,r-biocparallel)
       ("r-chipseq" ,r-chipseq)
       ("r-copyhelper" ,r-copyhelper)
       ("r-data-table" ,r-data-table)
       ("r-dnacopy" ,r-dnacopy)
       ("r-futile-logger" ,r-futile-logger)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicalignments" ,r-genomicalignments)
       ("r-genomicranges" ,r-genomicranges)
       ("r-gtools" ,r-gtools)
       ("r-iranges" ,r-iranges)
       ("r-matrixstats" ,r-matrixstats)
       ("r-rsamtools" ,r-rsamtools)
       ("r-s4vectors" ,r-s4vectors)))
    (home-page "https://github.com/PeeperLab/CopywriteR")
    (synopsis "Copy number information from targeted sequencing")
    (description
     "CopywriteR extracts DNA copy number information from targeted sequencing
by utilizing off-target reads.  It allows for extracting uniformly distributed
copy number information, can be used without reference, and can be applied to
sequencing data obtained from various techniques including chromatin
immunoprecipitation and target enrichment on small gene panels.  Thereby,
CopywriteR constitutes a widely applicable alternative to available copy
number detection tools.")
    (license license:gpl2)))

(define-public r-methylkit
  (package
    (name "r-methylkit")
    (version "1.14.2")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "methylKit" version))
              (sha256
               (base32
                "1qr13d2712ypbn96ijic2z5adr5dsd61kzscx7shw6vyj360rlm5"))))
    (properties `((upstream-name . "methylKit")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-data-table" ,r-data-table)
       ("r-emdbook" ,r-emdbook)
       ("r-fastseg" ,r-fastseg)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicranges" ,r-genomicranges)
       ("r-gtools" ,r-gtools)
       ("r-iranges" ,r-iranges)
       ("r-kernsmooth" ,r-kernsmooth)
       ("r-limma" ,r-limma)
       ("r-mclust" ,r-mclust)
       ("r-mgcv" ,r-mgcv)
       ("r-qvalue" ,r-qvalue)
       ("r-r-utils" ,r-r-utils)
       ("r-rcpp" ,r-rcpp)
       ("r-rhtslib" ,r-rhtslib)
       ("r-rsamtools" ,r-rsamtools)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-s4vectors" ,r-s4vectors)
       ("r-zlibbioc" ,r-zlibbioc)))
    (native-inputs
     `(("r-knitr" ,r-knitr))) ; for vignettes
    (inputs
     `(("zlib" ,zlib)))
    (home-page "https://github.com/al2na/methylKit")
    (synopsis
     "DNA methylation analysis from high-throughput bisulfite sequencing results")
    (description
     "MethylKit is an R package for DNA methylation analysis and annotation
from high-throughput bisulfite sequencing.  The package is designed to deal
with sequencing data from @dfn{Reduced representation bisulfite
sequencing} (RRBS) and its variants, but also target-capture methods and whole
genome bisulfite sequencing.  It also has functions to analyze base-pair
resolution 5hmC data from experimental protocols such as oxBS-Seq and
TAB-Seq.")
    (license license:artistic2.0)))

(define-public r-sva
  (package
    (name "r-sva")
    (version "3.36.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "sva" version))
       (sha256
        (base32
         "0xa1lm0k1a6nig90mab6xh4gln88rbs5l1cdr6ik6agg7jhs7ji4"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-edger" ,r-edger)
       ("r-genefilter" ,r-genefilter)
       ("r-mgcv" ,r-mgcv)
       ("r-biocparallel" ,r-biocparallel)
       ("r-matrixstats" ,r-matrixstats)
       ("r-limma" ,r-limma)))
    (home-page "https://bioconductor.org/packages/sva")
    (synopsis "Surrogate variable analysis")
    (description
     "This package contains functions for removing batch effects and other
unwanted variation in high-throughput experiment.  It also contains functions
for identifying and building surrogate variables for high-dimensional data
sets.  Surrogate variables are covariates constructed directly from
high-dimensional data like gene expression/RNA sequencing/methylation/brain
imaging data that can be used in subsequent analyses to adjust for unknown,
unmodeled, or latent sources of noise.")
    (license license:artistic2.0)))

(define-public r-raremetals2
  (package
    (name "r-raremetals2")
    (version "0.1")
    (source
     (origin
       (method url-fetch)
       (uri (string-append "http://genome.sph.umich.edu/w/images/"
                           "b/b7/RareMETALS2_" version ".tar.gz"))
       (sha256
        (base32
         "0z5ljcgvnm06ja9lm85a3cniq7slxcy37aqqkxrdidr79an5fs4s"))))
    (properties `((upstream-name . "RareMETALS2")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-seqminer" ,r-seqminer)
       ("r-mvtnorm" ,r-mvtnorm)
       ("r-mass" ,r-mass)
       ("r-compquadform" ,r-compquadform)
       ("r-getopt" ,r-getopt)))
    (home-page "http://genome.sph.umich.edu/wiki/RareMETALS2")
    (synopsis "Analyze gene-level association tests for binary trait")
    (description
     "The R package rareMETALS2 is an extension of the R package rareMETALS.
It was designed to meta-analyze gene-level association tests for binary trait.
While rareMETALS offers a near-complete solution for meta-analysis of
gene-level tests for quantitative trait, it does not offer the optimal
solution for binary trait.  The package rareMETALS2 offers improved features
for analyzing gene-level association tests in meta-analyses for binary
trait.")
    (license license:gpl3)))

(define-public r-protgenerics
  (package
    (name "r-protgenerics")
    (version "1.20.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "ProtGenerics" version))
       (sha256
        (base32
         "14xzdh7vxss8vmrw91hcwrszdn3ikm71mah8875b2lkrkrfzbl73"))))
    (properties `((upstream-name . "ProtGenerics")))
    (build-system r-build-system)
    (home-page "https://github.com/lgatto/ProtGenerics")
    (synopsis "S4 generic functions for proteomics infrastructure")
    (description
     "This package provides S4 generic functions needed by Bioconductor
proteomics packages.")
    (license license:artistic2.0)))

(define-public r-mzr
  (package
    (name "r-mzr")
    (version "2.22.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "mzR" version))
       (sha256
        (base32
         "1r8j8yiz5lcan7j4h37sza2kwczl48dxvld3da3ghjjq67cdc2cm"))
       (modules '((guix build utils)))
       (snippet
        '(begin
           (delete-file-recursively "src/boost")
           #t))))
    (properties `((upstream-name . "mzR")))
    (build-system r-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'use-system-boost
           (lambda _
             (substitute* "src/Makevars"
               (("\\./boost/libs.*") "")
               (("ARCH_OBJS=" line)
                (string-append line
                               "\nARCH_LIBS=-lboost_system -lboost_regex \
-lboost_iostreams -lboost_thread -lboost_filesystem -lboost_chrono\n")))
             #t)))))
    (inputs
     `(;; Our default boost package won't work here, unfortunately, even with
       ;; mzR version 2.22.0.
       ("boost" ,boost-for-mysql) ; use this instead of the bundled boost sources
       ("zlib" ,zlib)))
    (propagated-inputs
     `(("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-ncdf4" ,r-ncdf4)
       ("r-protgenerics" ,r-protgenerics)
       ("r-rcpp" ,r-rcpp)
       ("r-rhdf5lib" ,r-rhdf5lib)
       ("r-zlibbioc" ,r-zlibbioc)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://github.com/sneumann/mzR/")
    (synopsis "Parser for mass spectrometry data files")
    (description
     "The mzR package provides a unified API to the common file formats and
parsers available for mass spectrometry data.  It comes with a wrapper for the
ISB random access parser for mass spectrometry mzXML, mzData and mzML files.
The package contains the original code written by the ISB, and a subset of the
proteowizard library for mzML and mzIdentML.  The netCDF reading code has
previously been used in XCMS.")
    (license license:artistic2.0)))

(define-public r-affyio
  (package
    (name "r-affyio")
    (version "1.58.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "affyio" version))
       (sha256
        (base32
         "0j1f61409yq6hmkqrpzamfm7dx35rlq33ccs7wb1qcqx3d3nb75q"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-zlibbioc" ,r-zlibbioc)))
    (inputs
     `(("zlib" ,zlib)))
    (home-page "https://github.com/bmbolstad/affyio")
    (synopsis "Tools for parsing Affymetrix data files")
    (description
     "This package provides routines for parsing Affymetrix data files based
upon file format information.  The primary focus is on accessing the CEL and
CDF file formats.")
    (license license:lgpl2.0+)))

(define-public r-affy
  (package
    (name "r-affy")
    (version "1.66.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "affy" version))
       (sha256
        (base32
         "0m6hkyjxmsf80n3anhwh9k26csxczv6v92fkb7klnchdski61pyc"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-affyio" ,r-affyio)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-biocmanager" ,r-biocmanager)
       ("r-preprocesscore" ,r-preprocesscore)
       ("r-zlibbioc" ,r-zlibbioc)))
    (inputs
     `(("zlib" ,zlib)))
    (home-page "https://bioconductor.org/packages/affy")
    (synopsis "Methods for affymetrix oligonucleotide arrays")
    (description
     "This package contains functions for exploratory oligonucleotide array
analysis.")
    (license license:lgpl2.0+)))

(define-public r-vsn
  (package
    (name "r-vsn")
    (version "3.56.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "vsn" version))
       (sha256
        (base32
         "1k82dikrv1gcync5y1131wg7z1kxv2z2jl4nndg20bixc3398h58"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-affy" ,r-affy)
       ("r-biobase" ,r-biobase)
       ("r-ggplot2" ,r-ggplot2)
       ("r-lattice" ,r-lattice)
       ("r-limma" ,r-limma)))
    (native-inputs
     `(("r-knitr" ,r-knitr))) ; for vignettes
    (home-page "https://bioconductor.org/packages/release/bioc/html/vsn.html")
    (synopsis "Variance stabilization and calibration for microarray data")
    (description
     "The package implements a method for normalising microarray intensities,
and works for single- and multiple-color arrays.  It can also be used for data
from other technologies, as long as they have similar format.  The method uses
a robust variant of the maximum-likelihood estimator for an
additive-multiplicative error model and affine calibration.  The model
incorporates data calibration step (a.k.a.  normalization), a model for the
dependence of the variance on the mean intensity and a variance stabilizing
data transformation.  Differences between transformed intensities are
analogous to \"normalized log-ratios\".  However, in contrast to the latter,
their variance is independent of the mean, and they are usually more sensitive
and specific in detecting differential transcription.")
    (license license:artistic2.0)))

(define-public r-mzid
  (package
    (name "r-mzid")
    (version "1.26.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "mzID" version))
       (sha256
        (base32
         "0y50lzkdamkpz67f6r5whp246qsxpbammjil7g8vjprx0c4jk5n5"))))
    (properties `((upstream-name . "mzID")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-doparallel" ,r-doparallel)
       ("r-foreach" ,r-foreach)
       ("r-iterators" ,r-iterators)
       ("r-plyr" ,r-plyr)
       ("r-protgenerics" ,r-protgenerics)
       ("r-rcpp" ,r-rcpp)
       ("r-xml" ,r-xml)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/mzID")
    (synopsis "Parser for mzIdentML files")
    (description
     "This package provides a parser for mzIdentML files implemented using the
XML package.  The parser tries to be general and able to handle all types of
mzIdentML files with the drawback of having less pretty output than a vendor
specific parser.")
    (license license:gpl2+)))

(define-public r-pcamethods
  (package
    (name "r-pcamethods")
    (version "1.80.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "pcaMethods" version))
       (sha256
        (base32
         "10cww4jxyynkwxbbsx804nwac31j0hh8dgisygld0q663gaxkgni"))))
    (properties `((upstream-name . "pcaMethods")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-mass" ,r-mass)
       ("r-rcpp" ,r-rcpp)))
    (home-page "https://github.com/hredestig/pcamethods")
    (synopsis "Collection of PCA methods")
    (description
     "This package provides Bayesian PCA, Probabilistic PCA, Nipals PCA,
Inverse Non-Linear PCA and the conventional SVD PCA.  A cluster based method
for missing value estimation is included for comparison.  BPCA, PPCA and
NipalsPCA may be used to perform PCA on incomplete data as well as for
accurate missing value estimation.  A set of methods for printing and plotting
the results is also provided.  All PCA methods make use of the same data
structure (pcaRes) to provide a common interface to the PCA results.")
    (license license:gpl3+)))

(define-public r-msnbase
  (package
    (name "r-msnbase")
    (version "2.14.2")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "MSnbase" version))
       (sha256
        (base32
         "17vlv9gh41s1hp043b7j1jfqiw52alh1misjzy1kxl0g90rld00l"))))
    (properties `((upstream-name . "MSnbase")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-affy" ,r-affy)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-biocparallel" ,r-biocparallel)
       ("r-digest" ,r-digest)
       ("r-ggplot2" ,r-ggplot2)
       ("r-impute" ,r-impute)
       ("r-iranges" ,r-iranges)
       ("r-lattice" ,r-lattice)
       ("r-maldiquant" ,r-maldiquant)
       ("r-mass" ,r-mass)
       ("r-mzid" ,r-mzid)
       ("r-mzr" ,r-mzr)
       ("r-pcamethods" ,r-pcamethods)
       ("r-plyr" ,r-plyr)
       ("r-preprocesscore" ,r-preprocesscore)
       ("r-protgenerics" ,r-protgenerics)
       ("r-rcpp" ,r-rcpp)
       ("r-s4vectors" ,r-s4vectors)
       ("r-scales" ,r-scales)
       ("r-vsn" ,r-vsn)
       ("r-xml" ,r-xml)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://github.com/lgatto/MSnbase")
    (synopsis "Base functions and classes for MS-based proteomics")
    (description
     "This package provides basic plotting, data manipulation and processing
of mass spectrometry based proteomics data.")
    (license license:artistic2.0)))

(define-public r-msnid
  (package
    (name "r-msnid")
    (version "1.22.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "MSnID" version))
       (sha256
        (base32
         "0dwa6j2nqb3223a8g4f453aznjh69wngrpvdi12iy69j1psbbjcc"))))
    (properties `((upstream-name . "MSnID")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biobase" ,r-biobase)
       ("r-data-table" ,r-data-table)
       ("r-doparallel" ,r-doparallel)
       ("r-dplyr" ,r-dplyr)
       ("r-foreach" ,r-foreach)
       ("r-iterators" ,r-iterators)
       ("r-msnbase" ,r-msnbase)
       ("r-mzid" ,r-mzid)
       ("r-mzr" ,r-mzr)
       ("r-protgenerics" ,r-protgenerics)
       ("r-r-cache" ,r-r-cache)
       ("r-rcpp" ,r-rcpp)
       ("r-reshape2" ,r-reshape2)))
    (home-page "https://bioconductor.org/packages/MSnID")
    (synopsis "Utilities for LC-MSn proteomics identifications")
    (description
     "This package extracts @dfn{tandem mass spectrometry} (MS/MS) ID data
from mzIdentML (leveraging the mzID package) or text files.  After collating
the search results from multiple datasets it assesses their identification
quality and optimize filtering criteria to achieve the maximum number of
identifications while not exceeding a specified false discovery rate.  It also
contains a number of utilities to explore the MS/MS results and assess missed
and irregular enzymatic cleavages, mass measurement accuracy, etc.")
    (license license:artistic2.0)))

(define-public r-aroma-light
  (package
    (name "r-aroma-light")
    (version "3.18.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "aroma.light" version))
       (sha256
        (base32
         "19y5f2minx2pp73zdh43v1qkwpkaxygkl8cwlnwja15i46s0bcyc"))))
    (properties `((upstream-name . "aroma.light")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-matrixstats" ,r-matrixstats)
       ("r-r-methodss3" ,r-r-methodss3)
       ("r-r-oo" ,r-r-oo)
       ("r-r-utils" ,r-r-utils)))
    (home-page "https://github.com/HenrikBengtsson/aroma.light")
    (synopsis "Methods for normalization and visualization of microarray data")
    (description
     "This package provides methods for microarray analysis that take basic
data types such as matrices and lists of vectors.  These methods can be used
standalone, be utilized in other packages, or be wrapped up in higher-level
classes.")
    (license license:gpl2+)))

(define-public r-deseq
  (package
    (name "r-deseq")
    (version "1.39.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "DESeq" version))
       (sha256
        (base32
         "047hph5aqmjnz1aqprziw0smdn5lf96hmwpnvqrxv1j2yfvcf3h1"))))
    (properties `((upstream-name . "DESeq")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-genefilter" ,r-genefilter)
       ("r-geneplotter" ,r-geneplotter)
       ("r-lattice" ,r-lattice)
       ("r-locfit" ,r-locfit)
       ("r-mass" ,r-mass)
       ("r-rcolorbrewer" ,r-rcolorbrewer)))
    (home-page "https://www-huber.embl.de/users/anders/DESeq/")
    (synopsis "Differential gene expression analysis")
    (description
     "This package provides tools for estimating variance-mean dependence in
count data from high-throughput genetic sequencing assays and for testing for
differential expression based on a model using the negative binomial
distribution.")
    (license license:gpl3+)))

(define-public r-edaseq
  (package
    (name "r-edaseq")
    (version "2.22.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "EDASeq" version))
       (sha256
        (base32
         "12gzxjh73qshlwvsf92lbrf4bi199kxg2snrkprh1z4yqf7bjfm4"))))
    (properties `((upstream-name . "EDASeq")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-aroma-light" ,r-aroma-light)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-biocmanager" ,r-biocmanager)
       ("r-biomart" ,r-biomart)
       ("r-biostrings" ,r-biostrings)
       ("r-deseq" ,r-deseq)
       ("r-genomicfeatures" ,r-genomicfeatures)
       ("r-genomicranges" ,r-genomicranges)
       ("r-iranges" ,r-iranges)
       ("r-rsamtools" ,r-rsamtools)
       ("r-shortread" ,r-shortread)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://github.com/drisso/EDASeq")
    (synopsis "Exploratory data analysis and normalization for RNA-Seq")
    (description
     "This package provides support for numerical and graphical summaries of
RNA-Seq genomic read data.  Provided within-lane normalization procedures to
adjust for GC-content effect (or other gene-level effects) on read counts:
loess robust local regression, global-scaling, and full-quantile
normalization.  Between-lane normalization procedures to adjust for
distributional differences between lanes (e.g., sequencing depth):
global-scaling and full-quantile normalization.")
    (license license:artistic2.0)))

(define-public r-interactivedisplaybase
  (package
    (name "r-interactivedisplaybase")
    (version "1.26.3")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "interactiveDisplayBase" version))
       (sha256
        (base32
         "1x5vipqa4pgwpd62c1c58shnlpv3zyzzpf4wdwr00q1swkdb7wv3"))))
    (properties
     `((upstream-name . "interactiveDisplayBase")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-dt" ,r-dt)
       ("r-shiny" ,r-shiny)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/interactiveDisplayBase")
    (synopsis "Base package for web displays of Bioconductor objects")
    (description
     "This package contains the basic methods needed to generate interactive
Shiny-based display methods for Bioconductor objects.")
    (license license:artistic2.0)))

(define-public r-annotationhub
  (package
    (name "r-annotationhub")
    (version "2.20.2")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "AnnotationHub" version))
       (sha256
        (base32
         "04bz91m2wx1zm61rvpr0syyklz232fw74wrl73d965wi3x8fyda5"))))
    (properties `((upstream-name . "AnnotationHub")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-biocfilecache" ,r-biocfilecache)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-biocmanager" ,r-biocmanager)
       ("r-biocversion" ,r-biocversion)
       ("r-curl" ,r-curl)
       ("r-dplyr" ,r-dplyr)
       ("r-httr" ,r-httr)
       ("r-interactivedisplaybase" ,r-interactivedisplaybase)
       ("r-rappdirs" ,r-rappdirs)
       ("r-rsqlite" ,r-rsqlite)
       ("r-s4vectors" ,r-s4vectors)
       ("r-yaml" ,r-yaml)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/AnnotationHub")
    (synopsis "Client to access AnnotationHub resources")
    (description
     "This package provides a client for the Bioconductor AnnotationHub web
resource.  The AnnotationHub web resource provides a central location where
genomic files (e.g. VCF, bed, wig) and other resources from standard
locations (e.g. UCSC, Ensembl) can be discovered.  The resource includes
metadata about each resource, e.g., a textual description, tags, and date of
modification.  The client creates and manages a local cache of files retrieved
by the user, helping with quick and reproducible access.")
    (license license:artistic2.0)))

(define-public r-fastseg
  (package
    (name "r-fastseg")
    (version "1.34.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "fastseg" version))
       (sha256
        (base32
         "1d48n245pzmvcpsz93lxb4frqh222gfhpmlvm0sb74skn16way63"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-genomicranges" ,r-genomicranges)
       ("r-iranges" ,r-iranges)
       ("r-s4vectors" ,r-s4vectors)))
    (home-page "https://www.bioinf.jku.at/software/fastseg/index.html")
    (synopsis "Fast segmentation algorithm for genetic sequencing data")
    (description
     "Fastseg implements a very fast and efficient segmentation algorithm.
It can segment data from DNA microarrays and data from next generation
sequencing for example to detect copy number segments.  Further it can segment
data from RNA microarrays like tiling arrays to identify transcripts.  Most
generally, it can segment data given as a matrix or as a vector.  Various data
formats can be used as input to fastseg like expression set objects for
microarrays or GRanges for sequencing data.")
    (license license:lgpl2.0+)))

(define-public r-keggrest
  (package
    (name "r-keggrest")
    (version "1.28.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "KEGGREST" version))
       (sha256
        (base32
         "0q76w17fya2x0z7mvyhkk5kqh07flldgih13ma44vhcy1bdlm6j1"))))
    (properties `((upstream-name . "KEGGREST")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biostrings" ,r-biostrings)
       ("r-httr" ,r-httr)
       ("r-png" ,r-png)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/KEGGREST")
    (synopsis "Client-side REST access to KEGG")
    (description
     "This package provides a package that provides a client interface to the
@dfn{Kyoto Encyclopedia of Genes and Genomes} (KEGG) REST server.")
    (license license:artistic2.0)))

(define-public r-gage
  (package
    (name "r-gage")
    (version "2.38.3")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "gage" version))
       (sha256
        (base32
         "1bqmvjiya1df0b3h491lp1jxahiyidvaf9n094z0sk84x5y3xh2p"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-go-db" ,r-go-db)
       ("r-graph" ,r-graph)
       ("r-keggrest" ,r-keggrest)))
    (home-page (string-append "https://bmcbioinformatics.biomedcentral.com/"
                              "articles/10.1186/1471-2105-10-161"))
    (synopsis "Generally applicable gene-set enrichment for pathway analysis")
    (description
     "GAGE is a published method for gene set (enrichment or GSEA) or pathway
analysis.  GAGE is generally applicable independent of microarray or RNA-Seq
data attributes including sample sizes, experimental designs, assay platforms,
and other types of heterogeneity.  The gage package provides functions for
basic GAGE analysis, result processing and presentation.  In addition, it
provides demo microarray data and commonly used gene set data based on KEGG
pathways and GO terms.  These functions and data are also useful for gene set
analysis using other methods.")
    (license license:gpl2+)))

(define-public r-genomicfiles
  (package
    (name "r-genomicfiles")
    (version "1.24.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "GenomicFiles" version))
       (sha256
        (base32
         "1k3824pzf9fdqvcv6cz2742q3mabpmncrc72hwa21ac8wy1b04n4"))))
    (properties `((upstream-name . "GenomicFiles")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-biocparallel" ,r-biocparallel)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicalignments" ,r-genomicalignments)
       ("r-genomicranges" ,r-genomicranges)
       ("r-iranges" ,r-iranges)
       ("r-rsamtools" ,r-rsamtools)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-s4vectors" ,r-s4vectors)
       ("r-summarizedexperiment" ,r-summarizedexperiment)
       ("r-variantannotation" ,r-variantannotation)))
    (home-page "https://bioconductor.org/packages/GenomicFiles")
    (synopsis "Distributed computing by file or by range")
    (description
     "This package provides infrastructure for parallel computations
distributed by file or by range.  User defined mapper and reducer functions
provide added flexibility for data combination and manipulation.")
    (license license:artistic2.0)))

(define-public r-complexheatmap
  (package
    (name "r-complexheatmap")
    (version "2.4.3")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "ComplexHeatmap" version))
       (sha256
        (base32
         "1gx0hzrkla92pgmfkrm2zp0ccnhizq6rs26zgzpi5x8a5lvghh5q"))))
    (properties
     `((upstream-name . "ComplexHeatmap")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-circlize" ,r-circlize)
       ("r-clue" ,r-clue)
       ("r-colorspace" ,r-colorspace)
       ("r-getoptlong" ,r-getoptlong)
       ("r-globaloptions" ,r-globaloptions)
       ("r-png" ,r-png)
       ("r-rcolorbrewer" ,r-rcolorbrewer)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page
     "https://github.com/jokergoo/ComplexHeatmap")
    (synopsis "Making Complex Heatmaps")
    (description
     "Complex heatmaps are efficient to visualize associations between
different sources of data sets and reveal potential structures.  This package
provides a highly flexible way to arrange multiple heatmaps and supports
self-defined annotation graphics.")
    (license license:gpl2+)))

(define-public r-dirichletmultinomial
  (package
    (name "r-dirichletmultinomial")
    (version "1.30.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "DirichletMultinomial" version))
       (sha256
        (base32
         "1m9dsrddrllb2i88qzik1867iv9mggrgdkn0dlp8sq7gl69vmalb"))))
    (properties
     `((upstream-name . "DirichletMultinomial")))
    (build-system r-build-system)
    (inputs
     `(("gsl" ,gsl)))
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-iranges" ,r-iranges)
       ("r-s4vectors" ,r-s4vectors)))
    (home-page "https://bioconductor.org/packages/DirichletMultinomial")
    (synopsis "Dirichlet-Multinomial mixture models for microbiome data")
    (description
     "Dirichlet-multinomial mixture models can be used to describe variability
in microbial metagenomic data.  This package is an interface to code
originally made available by Holmes, Harris, and Quince, 2012, PLoS ONE 7(2):
1-15.")
    (license license:lgpl3)))

(define-public r-ensembldb
  (package
    (name "r-ensembldb")
    (version "2.12.1")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "ensembldb" version))
       (sha256
        (base32
         "1vvchc04nshxc768fp31rxb603aj3hmq8xlh5qabcwf2c3z9719g"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-annotationfilter" ,r-annotationfilter)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-biostrings" ,r-biostrings)
       ("r-curl" ,r-curl)
       ("r-dbi" ,r-dbi)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicfeatures" ,r-genomicfeatures)
       ("r-genomicranges" ,r-genomicranges)
       ("r-iranges" ,r-iranges)
       ("r-protgenerics" ,r-protgenerics)
       ("r-rsamtools" ,r-rsamtools)
       ("r-rsqlite" ,r-rsqlite)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-s4vectors" ,r-s4vectors)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://github.com/jotsetung/ensembldb")
    (synopsis "Utilities to create and use Ensembl-based annotation databases")
    (description
     "The package provides functions to create and use transcript-centric
annotation databases/packages.  The annotation for the databases are directly
fetched from Ensembl using their Perl API.  The functionality and data is
similar to that of the TxDb packages from the @code{GenomicFeatures} package,
but, in addition to retrieve all gene/transcript models and annotations from
the database, the @code{ensembldb} package also provides a filter framework
allowing to retrieve annotations for specific entries like genes encoded on a
chromosome region or transcript models of lincRNA genes.")
    ;; No version specified
    (license license:lgpl3+)))

(define-public r-organismdbi
  (package
    (name "r-organismdbi")
    (version "1.30.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "OrganismDbi" version))
       (sha256
        (base32
         "194h5576inq44qr666snzq0ygnc77rk5ljkn9bn8zs6x6gb3cwaw"))))
    (properties `((upstream-name . "OrganismDbi")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-biocmanager" ,r-biocmanager)
       ("r-dbi" ,r-dbi)
       ("r-genomicfeatures" ,r-genomicfeatures)
       ("r-genomicranges" ,r-genomicranges)
       ("r-graph" ,r-graph)
       ("r-iranges" ,r-iranges)
       ("r-rbgl" ,r-rbgl)
       ("r-s4vectors" ,r-s4vectors)))
    (home-page "https://bioconductor.org/packages/OrganismDbi")
    (synopsis "Software to enable the smooth interfacing of database packages")
    (description "The package enables a simple unified interface to several
annotation packages each of which has its own schema by taking advantage of
the fact that each of these packages implements a select methods.")
    (license license:artistic2.0)))

(define-public r-biovizbase
  (package
    (name "r-biovizbase")
    (version "1.36.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "biovizBase" version))
       (sha256
        (base32
         "1vq2mxa2jkljgw75zqjdkyml0ppi5dspvwj4cznfhi31cq8ds0qh"))))
    (properties `((upstream-name . "biovizBase")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-annotationfilter" ,r-annotationfilter)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-biostrings" ,r-biostrings)
       ("r-dichromat" ,r-dichromat)
       ("r-ensembldb" ,r-ensembldb)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicalignments" ,r-genomicalignments)
       ("r-genomicfeatures" ,r-genomicfeatures)
       ("r-genomicranges" ,r-genomicranges)
       ("r-hmisc" ,r-hmisc)
       ("r-iranges" ,r-iranges)
       ("r-rcolorbrewer" ,r-rcolorbrewer)
       ("r-rlang" ,r-rlang)
       ("r-rsamtools" ,r-rsamtools)
       ("r-s4vectors" ,r-s4vectors)
       ("r-scales" ,r-scales)
       ("r-summarizedexperiment" ,r-summarizedexperiment)
       ("r-variantannotation" ,r-variantannotation)))
    (home-page "https://bioconductor.org/packages/biovizBase")
    (synopsis "Basic graphic utilities for visualization of genomic data")
    (description
     "The biovizBase package is designed to provide a set of utilities, color
schemes and conventions for genomic data.  It serves as the base for various
high-level packages for biological data visualization.  This saves development
effort and encourages consistency.")
    (license license:artistic2.0)))

(define-public r-ggbio
  (package
    (name "r-ggbio")
    (version "1.36.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "ggbio" version))
       (sha256
        (base32
         "11ggnqjq42fi2hm9xlvrrlr2xhy4kglvl1a0mycp1s4v67lxw5h5"))))
    (build-system r-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         ;; See https://github.com/tengfei/ggbio/issues/117
         ;; This fix will be included in the next release.
         (add-after 'unpack 'fix-typo
           (lambda _
             (substitute* "R/GGbio-class.R"
               (("fechable") "fetchable"))
             #t)))))
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-annotationfilter" ,r-annotationfilter)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-biostrings" ,r-biostrings)
       ("r-biovizbase" ,r-biovizbase)
       ("r-bsgenome" ,r-bsgenome)
       ("r-ensembldb" ,r-ensembldb)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicalignments" ,r-genomicalignments)
       ("r-genomicfeatures" ,r-genomicfeatures)
       ("r-genomicranges" ,r-genomicranges)
       ("r-ggally" ,r-ggally)
       ("r-ggplot2" ,r-ggplot2)
       ("r-gridextra" ,r-gridextra)
       ("r-gtable" ,r-gtable)
       ("r-hmisc" ,r-hmisc)
       ("r-iranges" ,r-iranges)
       ("r-organismdbi" ,r-organismdbi)
       ("r-reshape2" ,r-reshape2)
       ("r-rlang" ,r-rlang)
       ("r-rsamtools" ,r-rsamtools)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-s4vectors" ,r-s4vectors)
       ("r-scales" ,r-scales)
       ("r-summarizedexperiment" ,r-summarizedexperiment)
       ("r-variantannotation" ,r-variantannotation)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "http://www.tengfei.name/ggbio/")
    (synopsis "Visualization tools for genomic data")
    (description
     "The ggbio package extends and specializes the grammar of graphics for
biological data.  The graphics are designed to answer common scientific
questions, in particular those often asked of high throughput genomics data.
All core Bioconductor data structures are supported, where appropriate.  The
package supports detailed views of particular genomic regions, as well as
genome-wide overviews.  Supported overviews include ideograms and grand linear
views.  High-level plots include sequence fragment length, edge-linked
interval to data view, mismatch pileup, and several splicing summaries.")
    (license license:artistic2.0)))

(define-public r-gqtlbase
  (package
    (name "r-gqtlbase")
    (version "1.20.4")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "gQTLBase" version))
       (sha256
        (base32
         "1ly14vhhqxjpbxjypi6ppd37dycabdhf4ny4nsvp9969k418zv41"))))
    (properties `((upstream-name . "gQTLBase")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-batchjobs" ,r-batchjobs)
       ("r-bbmisc" ,r-bbmisc)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-bit" ,r-bit)
       ("r-doparallel" ,r-doparallel)
       ("r-ff" ,r-ff)
       ("r-ffbase" ,r-ffbase)
       ("r-foreach" ,r-foreach)
       ("r-genomicfiles" ,r-genomicfiles)
       ("r-genomicranges" ,r-genomicranges)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-s4vectors" ,r-s4vectors)
       ("r-summarizedexperiment" ,r-summarizedexperiment)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/gQTLBase")
    (synopsis "Infrastructure for eQTL, mQTL and similar studies")
    (description
     "The purpose of this package is to simplify the storage and interrogation
of @dfn{quantitative trait loci} (QTL) archives, such as eQTL, mQTL, dsQTL,
and more.")
    (license license:artistic2.0)))

(define-public r-snpstats
  (package
    (name "r-snpstats")
    (version "1.38.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "snpStats" version))
       (sha256
        (base32
         "1qv3nqqr30d3n66mawqd9dbl95dl89r4bcjvkc5iassy1yrwr8wq"))))
    (properties `((upstream-name . "snpStats")))
    (build-system r-build-system)
    (inputs `(("zlib" ,zlib)))
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-matrix" ,r-matrix)
       ("r-survival" ,r-survival)
       ("r-zlibbioc" ,r-zlibbioc)))
    (home-page "https://bioconductor.org/packages/snpStats")
    (synopsis "Methods for SNP association studies")
    (description
     "This package provides classes and statistical methods for large
@dfn{single-nucleotide polymorphism} (SNP) association studies.  This extends
the earlier snpMatrix package, allowing for uncertainty in genotypes.")
    (license license:gpl3)))

(define-public r-homo-sapiens
  (package
    (name "r-homo-sapiens")
    (version "1.3.1")
    (source (origin
              (method url-fetch)
              ;; We cannot use bioconductor-uri here because this tarball is
              ;; located under "data/annotation/" instead of "bioc/".
              (uri (string-append "https://www.bioconductor.org/packages/"
                                  "release/data/annotation/src/contrib/"
                                  "Homo.sapiens_"
                                  version ".tar.gz"))
              (sha256
               (base32
                "151vj7h5p1c8yd5swrchk46z469p135wk50hvkl0nhgndvy0jj01"))))
    (properties
     `((upstream-name . "Homo.sapiens")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-genomicfeatures" ,r-genomicfeatures)
       ("r-go-db" ,r-go-db)
       ("r-org-hs-eg-db" ,r-org-hs-eg-db)
       ("r-txdb-hsapiens-ucsc-hg19-knowngene" ,r-txdb-hsapiens-ucsc-hg19-knowngene)
       ("r-organismdbi" ,r-organismdbi)
       ("r-annotationdbi" ,r-annotationdbi)))
    (home-page "https://bioconductor.org/packages/Homo.sapiens/")
    (synopsis "Annotation package for the Homo.sapiens object")
    (description
     "This package contains the Homo.sapiens object to access data from
several related annotation packages.")
    (license license:artistic2.0)))

(define-public r-erma
  (package
    (name "r-erma")
    (version "1.4.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "erma" version))
       (sha256
        (base32
         "1ccfbq0r48sr3h8050w8zv8402h7nx09adr0xdyqlg7kwp9vd2l3"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-biocparallel" ,r-biocparallel)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicfiles" ,r-genomicfiles)
       ("r-genomicranges" ,r-genomicranges)
       ("r-ggplot2" ,r-ggplot2)
       ("r-homo-sapiens" ,r-homo-sapiens)
       ("r-iranges" ,r-iranges)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-s4vectors" ,r-s4vectors)
       ("r-shiny" ,r-shiny)
       ("r-summarizedexperiment" ,r-summarizedexperiment)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/erma")
    (synopsis "Epigenomic road map adventures")
    (description
     "The epigenomics road map describes locations of epigenetic marks in DNA
from a variety of cell types.  Of interest are locations of histone
modifications, sites of DNA methylation, and regions of accessible chromatin.
This package presents a selection of elements of the road map including
metadata and outputs of the ChromImpute procedure applied to ENCODE cell lines
by Ernst and Kellis.")
    (license license:artistic2.0)))

(define-public r-ldblock
  (package
    (name "r-ldblock")
    (version "1.18.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "ldblock" version))
       (sha256
        (base32
         "0plw00n2zfgh029ab41dnydzgv2yxrapjp770147rx9pff4dngrv"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biocgenerics" ,r-biocgenerics)
       ("r-ensdb-hsapiens-v75" ,r-ensdb-hsapiens-v75)
       ("r-ensembldb" ,r-ensembldb)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicfiles" ,r-genomicfiles)
       ("r-httr" ,r-httr)
       ("r-matrix" ,r-matrix)
       ("r-rsamtools" ,r-rsamtools)
       ("r-snpstats" ,r-snpstats)
       ("r-variantannotation" ,r-variantannotation)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/ldblock")
    (synopsis "Data structures for linkage disequilibrium measures in populations")
    (description
     "This package defines data structures for @dfn{linkage
disequilibrium} (LD) measures in populations.  Its purpose is to simplify
handling of existing population-level data for the purpose of flexibly
defining LD blocks.")
    (license license:artistic2.0)))

(define-public r-gqtlstats
  (package
    (name "r-gqtlstats")
    (version "1.20.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "gQTLstats" version))
       (sha256
        (base32
         "1jjqfpjp93nmxjn757j5mzcax96bzcqdd1gr3rsdxg7ap008l2w7"))))
    (properties `((upstream-name . "gQTLstats")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-batchjobs" ,r-batchjobs)
       ("r-bbmisc" ,r-bbmisc)
       ("r-beeswarm" ,r-beeswarm)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-doparallel" ,r-doparallel)
       ("r-dplyr" ,r-dplyr)
       ("r-erma" ,r-erma)
       ("r-ffbase" ,r-ffbase)
       ("r-foreach" ,r-foreach)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicfeatures" ,r-genomicfeatures)
       ("r-genomicfiles" ,r-genomicfiles)
       ("r-genomicranges" ,r-genomicranges)
       ("r-ggbeeswarm" ,r-ggbeeswarm)
       ("r-ggplot2" ,r-ggplot2)
       ("r-gqtlbase" ,r-gqtlbase)
       ("r-hardyweinberg" ,r-hardyweinberg)
       ("r-homo-sapiens" ,r-homo-sapiens)
       ("r-iranges" ,r-iranges)
       ("r-limma" ,r-limma)
       ("r-mgcv" ,r-mgcv)
       ("r-plotly" ,r-plotly)
       ("r-reshape2" ,r-reshape2)
       ("r-s4vectors" ,r-s4vectors)
       ("r-shiny" ,r-shiny)
       ("r-snpstats" ,r-snpstats)
       ("r-summarizedexperiment" ,r-summarizedexperiment)
       ("r-variantannotation" ,r-variantannotation)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/gQTLstats")
    (synopsis "Computationally efficient analysis for eQTL and allied studies")
    (description
     "This package provides tools for the computationally efficient analysis
of @dfn{quantitative trait loci} (QTL) data, including eQTL, mQTL, dsQTL, etc.
The software in this package aims to support refinements and functional
interpretation of members of a collection of association statistics on a
family of feature/genome hypotheses.")
    (license license:artistic2.0)))

(define-public r-gviz
  (package
    (name "r-gviz")
    (version "1.32.0")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "Gviz" version))
       (sha256
        (base32
         "0cgkp0ciyy2qykqgh3vzp5mx9b4vsvacjh2jnsj3wldiapzlz08a"))))
    (properties `((upstream-name . "Gviz")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-biobase" ,r-biobase)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-biomart" ,r-biomart)
       ("r-biostrings" ,r-biostrings)
       ("r-biovizbase" ,r-biovizbase)
       ("r-bsgenome" ,r-bsgenome)
       ("r-digest" ,r-digest)
       ("r-ensembldb" ,r-ensembldb)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicalignments" ,r-genomicalignments)
       ("r-genomicfeatures" ,r-genomicfeatures)
       ("r-genomicranges" ,r-genomicranges)
       ("r-iranges" ,r-iranges)
       ("r-lattice" ,r-lattice)
       ("r-latticeextra" ,r-latticeextra)
       ("r-matrixstats" ,r-matrixstats)
       ("r-rcolorbrewer" ,r-rcolorbrewer)
       ("r-rsamtools" ,r-rsamtools)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-s4vectors" ,r-s4vectors)
       ("r-xvector" ,r-xvector)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/Gviz")
    (synopsis "Plotting data and annotation information along genomic coordinates")
    (description
     "Genomic data analyses requires integrated visualization of known genomic
information and new experimental data.  Gviz uses the biomaRt and the
rtracklayer packages to perform live annotation queries to Ensembl and UCSC
and translates this to e.g. gene/transcript structures in viewports of the
grid graphics package.  This results in genomic information plotted together
with your data.")
    (license license:artistic2.0)))

(define-public r-gwascat
  (package
    (name "r-gwascat")
    (version "2.20.1")
    (source
     (origin
       (method url-fetch)
       (uri (bioconductor-uri "gwascat" version))
       (sha256
        (base32
         "1cq5cmdrf0a0arr841yvkh6d8drc15p7mif1afr215l1s3y2dwd4"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-biocgenerics" ,r-biocgenerics)
       ("r-biostrings" ,r-biostrings)
       ("r-genomeinfodb" ,r-genomeinfodb)
       ("r-genomicfeatures" ,r-genomicfeatures)
       ("r-genomicranges" ,r-genomicranges)
       ("r-ggplot2" ,r-ggplot2)
       ("r-iranges" ,r-iranges)
       ("r-rsamtools" ,r-rsamtools)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-s4vectors" ,r-s4vectors)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://bioconductor.org/packages/gwascat")
    (synopsis "Tools for data in the EMBL-EBI GWAS catalog")
    (description
     "This package provides tools for representing and modeling data in the
EMBL-EBI GWAS catalog.")
    (license license:artistic2.0)))

(define-public r-sushi
  (package
    (name "r-sushi")
    (version "1.26.0")
    (source (origin
              (method url-fetch)
              (uri (bioconductor-uri "Sushi" version))
              (sha256
               (base32
                "17j3d5qjq5nbv99by5mq8rwr0jgh2jyyfn2nwxmwgzlmk3lgi1rb"))))
    (properties `((upstream-name . "Sushi")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-biomart" ,r-biomart)
       ("r-zoo" ,r-zoo)))
    (home-page "https://bioconductor.org/packages/Sushi")
    (synopsis "Tools for visualizing genomics data")
    (description
     "This package provides flexible, quantitative, and integrative genomic
visualizations for publication-quality multi-panel figures.")
    (license license:gpl2+)))

(define-public r-dropbead
  (let ((commit "d746c6f3b32110428ea56d6a0001ce52a251c247")
        (revision "2"))
    (package
      (name "r-dropbead")
      (version (string-append "0-" revision "." (string-take commit 7)))
      (source
       (origin
         (method git-fetch)
         (uri (git-reference
               (url "https://github.com/rajewsky-lab/dropbead")
               (commit commit)))
         (file-name (git-file-name name version))
         (sha256
          (base32
           "0sbzma49aiiyw8b0jpr7fnhzys9nsqmp4hy4hdz1gzyg1lhnca26"))))
      (build-system r-build-system)
      (propagated-inputs
       `(("r-ggplot2" ,r-ggplot2)
         ("r-rcolorbrewer" ,r-rcolorbrewer)
         ("r-gridextra" ,r-gridextra)
         ("r-gplots" ,r-gplots)
         ("r-plyr" ,r-plyr)))
      (home-page "https://github.com/rajewsky-lab/dropbead")
      (synopsis "Basic exploration and analysis of Drop-seq data")
      (description "This package offers a quick and straight-forward way to
explore and perform basic analysis of single cell sequencing data coming from
droplet sequencing.  It has been particularly tailored for Drop-seq.")
      (license license:gpl3))))

(define htslib-for-sambamba
  (let ((commit "2f3c3ea7b301f9b45737a793c0b2dcf0240e5ee5"))
    (package
      (inherit htslib)
      (name "htslib-for-sambamba")
      (version (string-append "1.3.1-1." (string-take commit 9)))
      (source
       (origin
         (method git-fetch)
         (uri (git-reference
               (url "https://github.com/lomereiter/htslib")
               (commit commit)))
         (file-name (string-append "htslib-" version "-checkout"))
         (sha256
          (base32
           "0g38g8s3npr0gjm9fahlbhiskyfws9l5i0x1ml3rakzj7az5l9c9"))))
      (native-inputs
       `(("autoconf" ,autoconf)
         ("automake" ,automake)
         ,@(package-native-inputs htslib))))))

(define-public sambamba
  (package
    (name "sambamba")
    (version "0.7.1")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/lomereiter/sambamba")
             (commit (string-append "v" version))))
       (file-name (string-append name "-" version "-checkout"))
       (sha256
        (base32
         "111h05b60pj8dxbidiamy4imc92x2962b3lmb7wgysl6lx064qis"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f                      ; there is no test target
       #:parallel-build? #f             ; not supported
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (add-after 'unpack 'fix-ldc-version
           (lambda _
             (substitute* "gen_ldc_version_info.py"
               (("/usr/bin/env.*") (which "python3")))
             (substitute* "Makefile"
               ;; We use ldc2 instead of ldmd2 to compile sambamba.
               (("\\$\\(shell which ldmd2\\)") (which "ldc2")))
             #t))
         (add-after 'unpack 'place-biod-and-undead
           (lambda* (#:key inputs #:allow-other-keys)
             (copy-recursively (assoc-ref inputs "biod") "BioD")
             #t))
         (add-after 'unpack 'unbundle-prerequisites
           (lambda _
             (substitute* "Makefile"
               (("htslib/libhts.a lz4/lib/liblz4.a")
                "-L-lhts -L-llz4")
               ((" lz4-static htslib-static") ""))
             #t))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out   (assoc-ref outputs "out"))
                    (bin   (string-append out "/bin")))
               (mkdir-p bin)
               (copy-file (string-append "bin/sambamba-" ,version)
                          (string-append bin "/sambamba"))
               #t))))))
    (native-inputs
     `(("ldc" ,ldc)
       ("rdmd" ,rdmd)
       ("python" ,python)
       ("biod"
        ,(let ((commit "7969eb0a847b05874e83ffddead26e193ece8101"))
           (origin
             (method git-fetch)
             (uri (git-reference
                   (url "https://github.com/biod/BioD")
                   (commit commit)))
             (file-name (string-append "biod-"
                                       (string-take commit 9)
                                       "-checkout"))
             (sha256
              (base32
               "0mjxsmbmv0jxl3pq21p8j5r829d648if8q58ka50b2956lc6qkpm")))))))
    (inputs
     `(("lz4" ,lz4)
       ("htslib" ,htslib-for-sambamba)))
    (home-page "https://lomereiter.github.io/sambamba/")
    (synopsis "Tools for working with SAM/BAM data")
    (description "Sambamba is a high performance modern robust and
fast tool (and library), written in the D programming language, for
working with SAM and BAM files.  Current parallelised functionality is
an important subset of samtools functionality, including view, index,
sort, markdup, and depth.")
    (license license:gpl2+)))

(define-public ritornello
  (package
    (name "ritornello")
    (version "2.0.1")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/KlugerLab/Ritornello")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1xahvq215qld7x1w8vpa5zbrsj6p9crb9shqa2x89sb0aaxa02jk"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f                      ; there are no tests
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'patch-samtools-references
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* '("src/SamStream.h"
                            "src/FLD.cpp")
               (("<sam.h>") "<samtools/sam.h>"))
             #t))
         (delete 'configure)
         (replace 'install
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let* ((out    (assoc-ref outputs "out"))
                    (bin    (string-append out "/bin/")))
               (mkdir-p bin)
               (install-file "bin/Ritornello" bin)
               #t))))))
    (inputs
     `(("samtools" ,samtools-0.1)
       ("fftw" ,fftw)
       ("boost" ,boost)
       ("zlib" ,zlib)))
    (home-page "https://github.com/KlugerLab/Ritornello")
    (synopsis "Control-free peak caller for ChIP-seq data")
    (description "Ritornello is a ChIP-seq peak calling algorithm based on
signal processing that can accurately call binding events without the need to
do a pair total DNA input or IgG control sample.  It has been tested for use
with narrow binding events such as transcription factor ChIP-seq.")
    (license license:gpl3+)))

(define-public trim-galore
  (package
    (name "trim-galore")
    (version "0.6.1")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/FelixKrueger/TrimGalore")
             (commit version)))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "1y31wbxwkm9xqzr5zv1pk5q418whnmlmgmfyxxpnl12h83m2i9iv"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f                      ; no tests
       #:phases
       (modify-phases %standard-phases
         (replace 'configure
           (lambda _
             ;; Trim Galore tries to figure out what version of Python
             ;; cutadapt is using by looking at the shebang.  Of course that
             ;; doesn't work, because cutadapt is wrapped in a shell script.
             (substitute* "trim_galore"
               (("my \\$python_return.*")
                "my $python_return = \"Python 3.999\";\n"))
             #t))
         (delete 'build)
         (add-after 'unpack 'hardcode-tool-references
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* "trim_galore"
               (("\\$path_to_cutadapt = 'cutadapt'")
                (string-append "$path_to_cutadapt = '"
                               (assoc-ref inputs "cutadapt")
                               "/bin/cutadapt'"))
               (("\\$compression_path = \"gzip\"")
                (string-append "$compression_path = \""
                               (assoc-ref inputs "gzip")
                               "/bin/gzip\""))
               (("\"gunzip")
                (string-append "\""
                               (assoc-ref inputs "gzip")
                               "/bin/gunzip"))
               (("\"pigz")
                (string-append "\""
                               (assoc-ref inputs "pigz")
                               "/bin/pigz")))
             #t))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((bin (string-append (assoc-ref outputs "out")
                                       "/bin")))
               (mkdir-p bin)
               (install-file "trim_galore" bin)
               #t))))))
    (inputs
     `(("gzip" ,gzip)
       ("perl" ,perl)
       ("pigz" ,pigz)
       ("cutadapt" ,cutadapt)))
    (native-inputs
     `(("unzip" ,unzip)))
    (home-page "https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/")
    (synopsis "Wrapper around Cutadapt and FastQC")
    (description "Trim Galore! is a wrapper script to automate quality and
adapter trimming as well as quality control, with some added functionality to
remove biased methylation positions for RRBS sequence files.")
    (license license:gpl3+)))

(define-public gess
  (package
    (name "gess")
    (version "1.0")
    (source (origin
              (method url-fetch)
              (uri (string-append "http://compbio.uthscsa.edu/"
                                  "GESS_Web/files/"
                                  "gess-" version ".src.tar.gz"))
              (sha256
               (base32
                "0hyk403kxscclzfs24pvdgiv0wm03kjcziqdrp5w46cb049gz0d7"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f                      ; no tests
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (delete 'build)
         (replace 'install
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let* ((python (assoc-ref inputs "python"))
                    (out    (assoc-ref outputs "out"))
                    (bin    (string-append out "/bin/"))
                    (target (string-append
                             out "/lib/python"
                             ,(version-major+minor
                                (package-version python))
                             "/site-packages/gess/")))
               (mkdir-p target)
               (copy-recursively "." target)
               ;; Make GESS.py executable
               (chmod (string-append target "GESS.py") #o555)
               ;; Add Python shebang to the top and make Matplotlib
               ;; usable.
               (substitute* (string-append target "GESS.py")
                 (("\"\"\"Description:" line)
                  (string-append "#!" (which "python") "
import matplotlib
matplotlib.use('Agg')
" line)))
               ;; Make sure GESS has all modules in its path
               (wrap-script (string-append target "GESS.py")
                 `("PYTHONPATH" ":" = (,target ,(getenv "PYTHONPATH"))))
               (mkdir-p bin)
               (symlink (string-append target "GESS.py")
                        (string-append bin "GESS.py"))
               #t))))))
    (inputs
     `(("python" ,python-2)
       ("python2-pysam" ,python2-pysam)
       ("python2-scipy" ,python2-scipy)
       ("python2-numpy" ,python2-numpy)
       ("python2-networkx" ,python2-networkx)
       ("python2-biopython" ,python2-biopython)
       ("guile" ,guile-3.0))) ; for the script wrapper
    (home-page "https://compbio.uthscsa.edu/GESS_Web/")
    (synopsis "Detect exon-skipping events from raw RNA-seq data")
    (description
     "GESS is an implementation of a novel computational method to detect de
novo exon-skipping events directly from raw RNA-seq data without the prior
knowledge of gene annotation information.  GESS stands for the graph-based
exon-skipping scanner detection scheme.")
    (license license:bsd-3)))

(define-public phylip
  (package
    (name "phylip")
    (version "3.696")
    (source
     (origin
       (method url-fetch)
       (uri (string-append "http://evolution.gs.washington.edu/phylip/"
                           "download/phylip-" version ".tar.gz"))
       (sha256
        (base32
         "01jar1rayhr2gba2pgbw49m56rc5z4p5wn3ds0m188hrlln4a2nd"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f                      ; no check target
       #:make-flags (list "-f" "Makefile.unx" "install")
       #:parallel-build? #f             ; not supported
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'enter-dir
           (lambda _ (chdir "src") #t))
         (delete 'configure)
         (replace 'install
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let ((target (string-append (assoc-ref outputs "out")
                                          "/bin")))
               (mkdir-p target)
               (for-each (lambda (file)
                           (install-file file target))
                         (find-files "../exe" ".*")))
             #t)))))
    (home-page "http://evolution.genetics.washington.edu/phylip/")
    (synopsis "Tools for inferring phylogenies")
    (description "PHYLIP (the PHYLogeny Inference Package) is a package of
programs for inferring phylogenies (evolutionary trees).")
    (license license:bsd-2)))

(define-public imp
  (package
    (name "imp")
    (version "2.13.0")
    (source
     (origin
       (method url-fetch)
       (uri (string-append "https://integrativemodeling.org/"
                           version "/download/imp-" version ".tar.gz"))
       (sha256
        (base32
         "1z1vcpwbylixk0zywngg5iw0jv083jj1bqphi817jpg3fb9fx2jj"))))
    (build-system cmake-build-system)
    (arguments
     `( ;; CMake 3.17 or newer is required for the CMAKE_TEST_ARGUMENTS used
       ;; below to have an effect.
       #:cmake ,cmake
       #:configure-flags
       (let ((disabled-tests
              '("expensive"                 ;exclude expensive tests
                "IMP.modeller"              ;fail to import its own modules
                "IMP.parallel-test_sge.py"  ;fail in build container
                ;; The following test fails non-reproducibly on
                ;; an inexact numbers assertion.
                "IMP.em-medium_test_local_fitting.py")))
         (list
          (string-append
           "-DCMAKE_CTEST_ARGUMENTS="
           (string-join
            (list "-L" "-tests?-"       ;select only tests
                  "-E" (format #f "'(~a)'" (string-join disabled-tests "|")))
            ";"))))))
    (native-inputs
     `(("python" ,python-wrapper)
       ("swig" ,swig)))
    (inputs
     `(("boost" ,boost)
       ("cgal" ,cgal)
       ("gsl" ,gsl)
       ("hdf5" ,hdf5)
       ("fftw" ,fftw)
       ("eigen" ,eigen)
       ;; Enabling MPI causes the build to use all the available memory and
       ;; fail (tested on a machine with 32 GiB of RAM).
       ;;("mpi" ,openmpi)
       ("opencv" ,opencv)))
    (propagated-inputs
     `(("python-numpy" ,python-numpy)
       ("python-scipy" ,python-scipy)
       ("python-pandas" ,python-pandas)
       ("python-scikit-learn" ,python-scikit-learn)
       ("python-networkx" ,python-networkx)))
    (home-page "https://integrativemodeling.org")
    (synopsis "Integrative modeling platform")
    (description "IMP's broad goal is to contribute to a comprehensive
structural characterization of biomolecules ranging in size and complexity
from small peptides to large macromolecular assemblies, by integrating data
from diverse biochemical and biophysical experiments.  IMP provides a C++ and
Python toolbox for solving complex modeling problems, and a number of
applications for tackling some common problems in a user-friendly way.")
    ;; IMP is largely available under the GNU Lesser GPL; see the file
    ;; COPYING.LGPL for the full text of this license. Some IMP modules are
    ;; available under the GNU GPL (see the file COPYING.GPL).
    (license (list license:lgpl2.1+
                   license:gpl3+))))

(define-public tadbit
  (package
    (name "tadbit")
    (version "1.0.1")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/3DGenomes/TADbit")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "0hqrlymh2a2bimcfdvlssy1x5h1lp3h1c5a7jj11hmcqczzqn3ni"))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'fix-problems-with-setup.py
           (lambda* (#:key outputs #:allow-other-keys)
             ;; Don't attempt to install the bash completions to
             ;; the home directory.
             (rename-file "extras/.bash_completion"
                          "extras/tadbit")
             (substitute* "setup.py"
               (("\\(path.expanduser\\('~'\\)")
                (string-append "(\""
                               (assoc-ref outputs "out")
                               "/etc/bash_completion.d\""))
               (("extras/\\.bash_completion")
                "extras/tadbit"))
             #t))
         (replace 'check
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (add-installed-pythonpath inputs outputs)
             (invoke "python3" "test/test_all.py")
             #t)))))
    (native-inputs
     `(("glib" ,glib "bin")             ;for gtester
       ("pkg-config" ,pkg-config)))
    (inputs
     ;; TODO: add Chimera for visualization
     `(("imp" ,imp)
       ("mcl" ,mcl)
       ("python-future" ,python-future)
       ("python-h5py" ,python-h5py)
       ("python-scipy" ,python-scipy)
       ("python-numpy" ,python-numpy)
       ("python-matplotlib" ,python-matplotlib)
       ("python-pysam" ,python-pysam)))
    (home-page "https://3dgenomes.github.io/TADbit/")
    (synopsis "Analyze, model, and explore 3C-based data")
    (description
     "TADbit is a complete Python library to deal with all steps to analyze,
model, and explore 3C-based data.  With TADbit the user can map FASTQ files to
obtain raw interaction binned matrices (Hi-C like matrices), normalize and
correct interaction matrices, identify and compare the so-called
@dfn{Topologically Associating Domains} (TADs), build 3D models from the
interaction matrices, and finally, extract structural properties from the
models.  TADbit is complemented by TADkit for visualizing 3D models.")
    (license license:gpl3+)))

(define-public kentutils
  (package
    (name "kentutils")
    ;; 302.1.0 is out, but the only difference is the inclusion of
    ;; pre-built binaries.
    (version "302.0.0")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/ENCODE-DCC/kentUtils")
             (commit (string-append "v" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "0n1wbyjpzii2b9qhyp9r1q76j623cggpg3y8fmw78ld3z4y7ivha"))
       (modules '((guix build utils)
                  (srfi srfi-26)
                  (ice-9 ftw)))
       (snippet
        '(begin
           ;; Only the contents of the specified directories are free
           ;; for all uses, so we remove the rest.  "hg/autoSql" and
           ;; "hg/autoXml" are nominally free, but they depend on a
           ;; library that is built from the sources in "hg/lib",
           ;; which is nonfree.
           (let ((free (list "." ".."
                             "utils" "lib" "inc" "tagStorm"
                             "parasol" "htslib"))
                 (directory? (lambda (file)
                               (eq? 'directory (stat:type (stat file))))))
             (for-each (lambda (file)
                         (and (directory? file)
                              (delete-file-recursively file)))
                       (map (cut string-append "src/" <>)
                            (scandir "src"
                                     (lambda (file)
                                       (not (member file free)))))))
           ;; Only make the utils target, not the userApps target,
           ;; because that requires libraries we won't build.
           (substitute* "Makefile"
             ((" userApps") " utils"))
           ;; Only build libraries that are free.
           (substitute* "src/makefile"
             (("DIRS =.*") "DIRS =\n")
             (("cd jkOwnLib.*") "")
             ((" hgLib") "")
             (("cd hg.*") ""))
           (substitute* "src/utils/makefile"
             ;; These tools depend on "jkhgap.a", which is part of the
             ;; nonfree "src/hg/lib" directory.
             (("raSqlQuery") "")
             (("pslLiftSubrangeBlat") "")

             ;; Do not build UCSC tools, which may require nonfree
             ;; components.
             (("ALL_APPS =.*") "ALL_APPS = $(UTILS_APPLIST)\n"))
           #t))))
    (build-system gnu-build-system)
    (arguments
     `( ;; There is no global test target and the test target for
       ;; individual tools depends on input files that are not
       ;; included.
       #:tests? #f
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'fix-permissions
           (lambda _ (make-file-writable "src/inc/localEnvironment.mk") #t))
         (add-after 'unpack 'fix-paths
           (lambda _
             (substitute* "Makefile"
               (("/bin/echo") (which "echo")))
             #t))
         (add-after 'unpack 'prepare-samtabix
           (lambda* (#:key inputs #:allow-other-keys)
             (copy-recursively (assoc-ref inputs "samtabix")
                               "samtabix")
             #t))
         (delete 'configure)
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((bin (string-append (assoc-ref outputs "out")
                                       "/bin")))
               (copy-recursively "bin" bin))
             #t)))))
    (native-inputs
     `(("samtabix"
        ,(origin
           (method git-fetch)
           (uri (git-reference
                 (url "http://genome-source.cse.ucsc.edu/samtabix.git")
                 (commit "10fd107909c1ac4d679299908be4262a012965ba")))
           (sha256
            (base32
             "0c1nj64l42v395sa84n7az43xiap4i6f9n9dfz4058aqiwkhkmma"))))))
    (inputs
     `(("zlib" ,zlib)
       ("tcsh" ,tcsh)
       ("perl" ,perl)
       ("libpng" ,libpng)
       ("mariadb-dev" ,mariadb "dev")
       ("openssl" ,openssl-1.0)))
    (home-page "https://genome.cse.ucsc.edu/index.html")
    (synopsis "Assorted bioinformatics utilities")
    (description "This package provides the kentUtils, a selection of
bioinformatics utilities used in combination with the UCSC genome
browser.")
    ;; Only a subset of the sources are released under a non-copyleft
    ;; free software license.  All other sources are removed in a
    ;; snippet.  See this bug report for an explanation of how the
    ;; license statements apply:
    ;; https://github.com/ENCODE-DCC/kentUtils/issues/12
    (license (license:non-copyleft
              "http://genome.ucsc.edu/license/"
              "The contents of this package are free for all uses."))))

(define-public f-seq
  (let ((commit "6ccded34cff38cf432deed8503648b4a66953f9b")
        (revision "1"))
    (package
      (name "f-seq")
      (version (string-append "1.1-" revision "." (string-take commit 7)))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "https://github.com/aboyle/F-seq")
                      (commit commit)))
                (file-name (string-append name "-" version))
                (sha256
                 (base32
                  "1nk33k0yajg2id4g59bc4szr58r2q6pdq42vgcw054m8ip9wv26h"))
                (modules '((guix build utils)))
                ;; Remove bundled Java library archives.
                (snippet
                 '(begin
                    (for-each delete-file (find-files "lib" ".*"))
                    #t))))
      (build-system ant-build-system)
      (arguments
       `(#:tests? #f ; no tests included
         #:phases
         (modify-phases %standard-phases
           (replace 'install
             (lambda* (#:key inputs outputs #:allow-other-keys)
               (let* ((target (assoc-ref outputs "out"))
                      (bin (string-append target "/bin"))
                      (doc (string-append target "/share/doc/f-seq"))
                      (lib (string-append target "/lib")))
                 (mkdir-p target)
                 (mkdir-p doc)
                 (substitute* "bin/linux/fseq"
                   (("java") (which "java"))
                   (("\\$REALDIR/../lib/commons-cli-1.1.jar")
                    (string-append (assoc-ref inputs "java-commons-cli")
                                   "/share/java/commons-cli.jar"))
                   (("REALDIR=.*")
                    (string-append "REALDIR=" bin "\n")))
                 (install-file "README.txt" doc)
                 (install-file "bin/linux/fseq" bin)
                 (install-file "build~/fseq.jar" lib)
                 (copy-recursively "lib" lib)
                 #t))))))
      (inputs
       `(("perl" ,perl)
         ("java-commons-cli" ,java-commons-cli)))
      (home-page "http://fureylab.web.unc.edu/software/fseq/")
      (synopsis "Feature density estimator for high-throughput sequence tags")
      (description
       "F-Seq is a software package that generates a continuous tag sequence
density estimation allowing identification of biologically meaningful sites
such as transcription factor binding sites (ChIP-seq) or regions of open
chromatin (DNase-seq).  Output can be displayed directly in the UCSC Genome
Browser.")
      (license license:gpl3+))))

(define-public bismark
  (package
    (name "bismark")
    (version "0.20.1")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/FelixKrueger/Bismark")
             (commit version)))
       (file-name (string-append name "-" version "-checkout"))
       (sha256
        (base32
         "0xchm3rgilj6vfjnyzfzzymfd7djr64sbrmrvs3njbwi66jqbzw9"))))
    (build-system perl-build-system)
    (arguments
     `(#:tests? #f                      ; there are no tests
       #:modules ((guix build utils)
                  (ice-9 popen)
                  (srfi srfi-26)
                  (guix build perl-build-system))
       #:phases
       (modify-phases %standard-phases
         ;; The bundled plotly.js is minified.
         (add-after 'unpack 'replace-plotly.js
           (lambda* (#:key inputs #:allow-other-keys)
             (let* ((file (assoc-ref inputs "plotly.js"))
                    (installed "plotly/plotly.js"))
               (let ((minified (open-pipe* OPEN_READ "uglify-js" file)))
                 (call-with-output-file installed
                   (cut dump-port minified <>))))
             #t))
         (delete 'configure)
         (delete 'build)
         (replace 'install
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin"))
                    (share   (string-append out "/share/bismark"))
                    (docdir  (string-append out "/share/doc/bismark"))
                    (docs    '("Docs/Bismark_User_Guide.html"))
                    (scripts '("bismark"
                               "bismark_genome_preparation"
                               "bismark_methylation_extractor"
                               "bismark2bedGraph"
                               "bismark2report"
                               "coverage2cytosine"
                               "deduplicate_bismark"
                               "filter_non_conversion"
                               "bam2nuc"
                               "bismark2summary"
                               "NOMe_filtering")))
               (substitute* "bismark2report"
                 (("\\$RealBin/plotly")
                  (string-append share "/plotly")))
               (mkdir-p share)
               (mkdir-p docdir)
               (mkdir-p bin)
               (for-each (lambda (file) (install-file file bin))
                         scripts)
               (for-each (lambda (file) (install-file file docdir))
                         docs)
               (copy-recursively "Docs/Images" (string-append docdir "/Images"))
               (copy-recursively "plotly"
                                 (string-append share "/plotly"))

               ;; Fix references to gunzip
               (substitute* (map (lambda (file)
                                   (string-append bin "/" file))
                                 scripts)
                 (("\"gunzip -c")
                  (string-append "\"" (assoc-ref inputs "gzip")
                                 "/bin/gunzip -c")))
               #t))))))
    (inputs
     `(("gzip" ,gzip)
       ("perl-carp" ,perl-carp)
       ("perl-getopt-long" ,perl-getopt-long)))
    (native-inputs
     `(("plotly.js"
        ,(origin
           (method url-fetch)
           (uri (string-append "https://raw.githubusercontent.com/plotly/plotly.js/"
                               "v1.39.4/dist/plotly.js"))
           (sha256
            (base32 "138mwsr4nf5qif4mrxx286mpnagxd1xwl6k8aidrjgknaqg88zyr"))))
       ("uglify-js" ,uglify-js)))
    (home-page "https://www.bioinformatics.babraham.ac.uk/projects/bismark/")
    (synopsis "Map bisulfite treated sequence reads and analyze methylation")
    (description "Bismark is a program to map bisulfite treated sequencing
reads to a genome of interest and perform methylation calls in a single step.
The output can be easily imported into a genome viewer, such as SeqMonk, and
enables a researcher to analyse the methylation levels of their samples
straight away.  Its main features are:

@itemize
@item Bisulfite mapping and methylation calling in one single step
@item Supports single-end and paired-end read alignments
@item Supports ungapped and gapped alignments
@item Alignment seed length, number of mismatches etc are adjustable
@item Output discriminates between cytosine methylation in CpG, CHG
  and CHH context
@end itemize\n")
    (license license:gpl3+)))

(define-public paml
  (package
    (name "paml")
    (version "4.9e")
    (source (origin
              (method url-fetch)
              (uri (string-append "http://abacus.gene.ucl.ac.uk/software/"
                                  "paml" version ".tgz"))
              (sha256
               (base32
                "13zf6h9fiqghwhch2h06x1zdr6s42plsnqahflp5g7myr3han3s6"))
              (modules '((guix build utils)))
              ;; Remove Windows binaries
              (snippet
               '(begin
                  (for-each delete-file (find-files "." "\\.exe$"))
                  ;; Some files in the original tarball have restrictive
                  ;; permissions, which makes repackaging fail
                  (for-each (lambda (file) (chmod file #o644)) (find-files "."))
                  #t))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; there are no tests
       #:make-flags '("CC=gcc")
       #:phases
       (modify-phases %standard-phases
         (replace 'configure
           (lambda _
             (substitute* "src/BFdriver.c"
               (("/bin/bash") (which "bash")))
             (chdir "src")
             #t))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((tools '("baseml" "basemlg" "codeml"
                            "pamp" "evolver" "yn00" "chi2"))
                   (bin    (string-append (assoc-ref outputs "out") "/bin"))
                   (docdir (string-append (assoc-ref outputs "out")
                                           "/share/doc/paml")))
               (mkdir-p bin)
               (for-each (lambda (file) (install-file file bin)) tools)
               (copy-recursively "../doc" docdir)
               #t))))))
    (home-page "http://abacus.gene.ucl.ac.uk/software/paml.html")
    (synopsis "Phylogentic analysis by maximum likelihood")
    (description "PAML (for Phylogentic Analysis by Maximum Likelihood)
contains a few programs for model fitting and phylogenetic tree reconstruction
using nucleotide or amino-acid sequence data.")
    ;; GPLv3 only
    (license license:gpl3)))

(define-public kallisto
  (package
    (name "kallisto")
    (version "0.44.0")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/pachterlab/kallisto")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "0nj382jiywqnpgvyhichajpkkh5r0bapn43f4dx40zdaq5v4m40m"))))
    (build-system cmake-build-system)
    (arguments
     `(#:tests? #f          ; no "check" target
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'do-not-use-bundled-htslib
           (lambda _
             (substitute* "CMakeLists.txt"
               (("^ExternalProject_Add" m)
                (string-append "if (NEVER)\n" m))
               (("^\\)")
                (string-append ")\nendif(NEVER)"))
               (("include_directories\\(\\$\\{htslib_PREFIX.*" m)
                (string-append "# " m)))
             (substitute* "src/CMakeLists.txt"
               (("target_link_libraries\\(kallisto kallisto_core pthread \
\\$\\{CMAKE_CURRENT_SOURCE_DIR\\}/../ext/htslib/libhts.a\\)")
                "target_link_libraries(kallisto kallisto_core pthread hts)")
               (("include_directories\\(\\.\\./ext/htslib\\)") ""))
             #t)))))
    (inputs
     `(("hdf5" ,hdf5)
       ("htslib" ,htslib)
       ("zlib" ,zlib)))
    (home-page "https://pachterlab.github.io/kallisto/")
    (synopsis "Near-optimal RNA-Seq quantification")
    (description
     "Kallisto is a program for quantifying abundances of transcripts from
RNA-Seq data, or more generally of target sequences using high-throughput
sequencing reads.  It is based on the novel idea of pseudoalignment for
rapidly determining the compatibility of reads with targets, without the need
for alignment.  Pseudoalignment of reads preserves the key information needed
for quantification, and kallisto is therefore not only fast, but also as
accurate as existing quantification tools.")
    (license license:bsd-2)))

(define-public libgff
  (package
    (name "libgff")
    (version "1.0")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/Kingsford-Group/libgff")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "0n6vfjnq7a2mianipscbshrvbncss8z4zkgkbjw754p9043nfkps"))))
    (build-system cmake-build-system)
    (arguments `(#:tests? #f))          ; no tests included
    (home-page "https://github.com/Kingsford-Group/libgff")
    (synopsis "Parser library for reading/writing GFF files")
    (description "This is a simple \"libraryfication\" of the GFF/GTF parsing
code that is used in the Cufflinks codebase.  The goal of this library is to
provide this functionality without the necessity of drawing in a heavy-weight
dependency like SeqAn.")
    (license (license:x11-style "https://www.boost.org/LICENSE_1_0.txt"))))

(define-public sailfish
  (package
    (name "sailfish")
    (version "0.10.1")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/kingsfordgroup/sailfish")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1amcc5hqvsl42hg4x19bi9vy47cl874s0lw1fmi0hwsdk9i8c03v"))
              (modules '((guix build utils)))
              (snippet
               '(begin
                  ;; Delete bundled headers for eigen3.
                  (delete-file-recursively "include/eigen3/")
                  #t))))
    (build-system cmake-build-system)
    (arguments
     `(#:configure-flags
       (list (string-append "-DBOOST_INCLUDEDIR="
                            (assoc-ref %build-inputs "boost")
                            "/include/")
             (string-append "-DBOOST_LIBRARYDIR="
                            (assoc-ref %build-inputs "boost")
                            "/lib/")
             (string-append "-DBoost_LIBRARIES="
                            "-lboost_iostreams "
                            "-lboost_filesystem "
                            "-lboost_system "
                            "-lboost_thread "
                            "-lboost_timer "
                            "-lboost_chrono "
                            "-lboost_program_options")
             "-DBoost_FOUND=TRUE"
             ;; Don't download RapMap---we already have it!
             "-DFETCHED_RAPMAP=1")
       ;; Tests must be run after installation and the location of the test
       ;; data file must be overridden.  But the tests fail.  It looks like
       ;; they are not really meant to be run.
       #:tests? #f
       #:phases
       (modify-phases %standard-phases
         ;; Boost cannot be found, even though it's right there.
         (add-after 'unpack 'do-not-look-for-boost
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* "CMakeLists.txt"
               (("find_package\\(Boost 1\\.53\\.0") "#"))
             #t))
         (add-after 'unpack 'do-not-assign-to-macro
           (lambda _
             (substitute* "include/spdlog/details/format.cc"
               (("const unsigned CHAR_WIDTH = 1;") ""))
             #t))
         (add-after 'unpack 'prepare-rapmap
           (lambda* (#:key inputs #:allow-other-keys)
             (let ((src "external/install/src/rapmap/")
                   (include "external/install/include/rapmap/")
                   (rapmap (assoc-ref inputs "rapmap")))
               (mkdir-p "/tmp/rapmap")
               (invoke "tar" "xf"
                       (assoc-ref inputs "rapmap")
                       "-C" "/tmp/rapmap"
                       "--strip-components=1")
               (mkdir-p src)
               (mkdir-p include)
               (for-each (lambda (file)
                           (install-file file src))
                         (find-files "/tmp/rapmap/src" "\\.(c|cpp)"))
               (copy-recursively "/tmp/rapmap/include" include))
             #t))
         (add-after 'unpack 'use-system-libraries
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* '("src/SailfishIndexer.cpp"
                            "src/SailfishUtils.cpp"
                            "src/SailfishQuantify.cpp"
                            "src/FASTAParser.cpp"
                            "include/PCA.hpp"
                            "include/SailfishUtils.hpp"
                            "include/SailfishIndex.hpp"
                            "include/CollapsedEMOptimizer.hpp"
                            "src/CollapsedEMOptimizer.cpp")
               (("#include \"jellyfish/config.h\"") ""))
             (substitute* "src/CMakeLists.txt"
               (("\\$\\{GAT_SOURCE_DIR\\}/external/install/include/jellyfish-2.2..")
                (string-append (assoc-ref inputs "jellyfish")
                               "/include/jellyfish-" ,(package-version jellyfish)))
               (("\\$\\{GAT_SOURCE_DIR\\}/external/install/lib/libjellyfish-2.0.a")
                (string-append (assoc-ref inputs "jellyfish")
                               "/lib/libjellyfish-2.0.a"))
               (("\\$\\{GAT_SOURCE_DIR\\}/external/install/lib/libdivsufsort.a")
                (string-append (assoc-ref inputs "libdivsufsort")
                               "/lib/libdivsufsort.so"))
               (("\\$\\{GAT_SOURCE_DIR\\}/external/install/lib/libdivsufsort64.a")
                (string-append (assoc-ref inputs "libdivsufsort")
                               "/lib/libdivsufsort64.so")))
             (substitute* "CMakeLists.txt"
               ;; Don't prefer static libs
               (("SET\\(CMAKE_FIND_LIBRARY_SUFFIXES.*") "")
               (("find_package\\(Jellyfish.*") "")
               (("ExternalProject_Add\\(libjellyfish") "message(")
               (("ExternalProject_Add\\(libgff") "message(")
               (("ExternalProject_Add\\(libsparsehash") "message(")
               (("ExternalProject_Add\\(libdivsufsort") "message("))

             ;; Ensure that Eigen headers can be found
             (setenv "CPLUS_INCLUDE_PATH"
                     (string-append (assoc-ref inputs "eigen")
                                    "/include/eigen3:"
                                    (or (getenv "CPLUS_INCLUDE_PATH") "")))
             #t)))))
    (inputs
     `(("boost" ,boost)
       ("eigen" ,eigen)
       ("jemalloc" ,jemalloc)
       ("jellyfish" ,jellyfish)
       ("sparsehash" ,sparsehash)
       ("rapmap" ,(origin
                    (method git-fetch)
                    (uri (git-reference
                          (url "https://github.com/COMBINE-lab/RapMap")
                          (commit (string-append "sf-v" version))))
                    (file-name (string-append "rapmap-sf-v" version "-checkout"))
                    (sha256
                     (base32
                      "1hv79l5i576ykv5a1srj2p0q36yvyl5966m0fcy2lbi169ipjakf"))
                    (modules '((guix build utils)))
                    ;; These files are expected to be excluded.
                    (snippet
                     '(begin (delete-file-recursively "include/spdlog")
                             (for-each delete-file '("include/xxhash.h"
                                                     "src/xxhash.c"))
                             #t))))
       ("libdivsufsort" ,libdivsufsort)
       ("libgff" ,libgff)
       ("tbb" ,tbb)
       ("zlib" ,zlib)))
    (native-inputs
     `(("pkg-config" ,pkg-config)))
    (home-page "https://www.cs.cmu.edu/~ckingsf/software/sailfish/")
    (synopsis "Mapping-based isoform quantification from RNA-Seq reads")
    (description "Sailfish is a tool for genomic transcript quantification
from RNA-seq data.  It requires a set of target transcripts (either from a
reference or de-novo assembly) to quantify.  All you need to run sailfish is a
fasta file containing your reference transcripts and a (set of) fasta/fastq
file(s) containing your reads.")
    (license license:gpl3+)))

(define libstadenio-for-salmon
  (package
    (name "libstadenio")
    (version "1.14.8")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/COMBINE-lab/staden-io_lib")
                    (commit (string-append "v" version))))
              (file-name (string-append name "-" version "-checkout"))
              (sha256
               (base32
                "1x8kxxqxl892vwfbprlbyfwkkv7c34ggkc94892x9x0g37x5nbwx"))))
    (build-system gnu-build-system)
    (arguments '(#:parallel-tests? #f)) ; not supported
    (inputs
     `(("zlib" ,zlib)))
    (native-inputs
     `(("perl" ,perl)))                 ; for tests
    (home-page "https://github.com/COMBINE-lab/staden-io_lib")
    (synopsis "General purpose trace and experiment file library")
    (description "This package provides a library of file reading and writing
code to provide a general purpose Trace file (and Experiment File) reading
interface.

The following file formats are supported:

@enumerate
@item SCF trace files
@item ABI trace files
@item ALF trace files
@item ZTR trace files
@item SFF trace archives
@item SRF trace archives
@item Experiment files
@item Plain text files
@item SAM/BAM sequence files
@item CRAM sequence files
@end enumerate\n")
    (license license:bsd-3)))

(define-public salmon
  (package
    (name "salmon")
    (version "0.13.1")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/COMBINE-lab/salmon")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1i2z4aivicmiixdz9bxalp7vmfzi3k92fxa63iqa8kgvfw5a4aq5"))
              (modules '((guix build utils)))
              (snippet
               '(begin
                  ;; Delete bundled headers for eigen3.
                  (delete-file-recursively "include/eigen3/")
                  #t))))
    (build-system cmake-build-system)
    (arguments
     `(#:configure-flags
       (list (string-append "-DBOOST_INCLUDEDIR="
                            (assoc-ref %build-inputs "boost")
                            "/include/")
             (string-append "-DBOOST_LIBRARYDIR="
                            (assoc-ref %build-inputs "boost")
                            "/lib/")
             (string-append "-DBoost_LIBRARIES="
                            "-lboost_iostreams "
                            "-lboost_filesystem "
                            "-lboost_system "
                            "-lboost_thread "
                            "-lboost_timer "
                            "-lboost_chrono "
                            "-lboost_program_options")
             "-DBoost_FOUND=TRUE"
             "-DTBB_LIBRARIES=tbb tbbmalloc"
             ;; Don't download RapMap---we already have it!
             "-DFETCHED_RAPMAP=1")
       #:phases
       (modify-phases %standard-phases
         ;; Boost cannot be found, even though it's right there.
         (add-after 'unpack 'do-not-look-for-boost
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* "CMakeLists.txt"
               (("find_package\\(Boost 1\\.59\\.0") "#"))
             #t))
         (add-after 'unpack 'do-not-phone-home
           (lambda _
             (substitute* "src/Salmon.cpp"
               (("getVersionMessage\\(\\)") "\"\""))
             #t))
         (add-after 'unpack 'prepare-rapmap
           (lambda* (#:key inputs #:allow-other-keys)
             (let ((src "external/install/src/rapmap/")
                   (include "external/install/include/rapmap/")
                   (rapmap (assoc-ref inputs "rapmap")))
               (mkdir-p src)
               (mkdir-p include)
               (copy-recursively (string-append rapmap "/src") src)
               (copy-recursively (string-append rapmap "/include") include)
               (for-each delete-file '("external/install/include/rapmap/xxhash.h"
                                       "external/install/include/rapmap/FastxParser.hpp"
                                       "external/install/include/rapmap/concurrentqueue.h"
                                       "external/install/include/rapmap/FastxParserThreadUtils.hpp"
                                       "external/install/src/rapmap/FastxParser.cpp"
                                       "external/install/src/rapmap/xxhash.c"))
               (delete-file-recursively "external/install/include/rapmap/spdlog"))
             #t))
         (add-after 'unpack 'use-system-libraries
           (lambda* (#:key inputs #:allow-other-keys)
             (substitute* "CMakeLists.txt"
               ;; Don't prefer static libs
               (("SET\\(CMAKE_FIND_LIBRARY_SUFFIXES.*") "")
               (("set\\(TBB_LIBRARIES") "message(")
               ;; Don't download anything
               (("DOWNLOAD_COMMAND") "DOWNLOAD_COMMAND echo")
               (("externalproject_add\\(libcereal") "message(")
               (("externalproject_add\\(libgff") "message(")
               (("externalproject_add\\(libtbb") "message(")
               (("externalproject_add\\(libdivsufsort") "message(")
               (("externalproject_add\\(libstadenio") "message(")
               (("externalproject_add_step\\(") "message("))
             (substitute* "src/CMakeLists.txt"
               (("add_dependencies") "#")
               (("\\$\\{GAT_SOURCE_DIR\\}/external/install/lib/libstaden-read.a")
                (string-append (assoc-ref inputs "libstadenio-for-salmon")
                               "/lib/libstaden-read.so"))
               (("\\$\\{GAT_SOURCE_DIR\\}/external/install/lib/libdivsufsort.a")
                (string-append (assoc-ref inputs "libdivsufsort")
                               "/lib/libdivsufsort.so"))
               (("\\$\\{GAT_SOURCE_DIR\\}/external/install/lib/libdivsufsort64.a")
                (string-append (assoc-ref inputs "libdivsufsort")
                               "/lib/libdivsufsort64.so"))
               (("lib/libdivsufsort.a") "/lib/libdivsufsort.so"))

             ;; Ensure that all headers can be found
             (setenv "CPLUS_INCLUDE_PATH"
                     (string-append (or (getenv "CPLUS_INCLUDE_PATH") "")
                                    ":"
                                    (assoc-ref inputs "eigen")
                                    "/include/eigen3"))
             #t))
         ;; CMAKE_INSTALL_PREFIX does not exist when the tests are
         ;; run.  It only exists after the install phase.
         (add-after 'unpack 'fix-tests
           (lambda _
             (substitute* "src/CMakeLists.txt"
               (("DTOPLEVEL_DIR=\\$\\{CMAKE_INSTALL_PREFIX")
                "DTOPLEVEL_DIR=${GAT_SOURCE_DIR"))
             #t)))))
    (inputs
     `(("boost" ,boost)
       ("bzip2" ,bzip2)
       ("cereal" ,cereal)
       ("eigen" ,eigen)
       ("rapmap" ,(origin
                    (method git-fetch)
                    (uri (git-reference
                          (url "https://github.com/COMBINE-lab/RapMap")
                          (commit (string-append "salmon-v" version))))
                    (file-name (string-append "rapmap-salmon-v" version "-checkout"))
                    (sha256
                     (base32
                      "1biplxf0csc7a8h1wf219b0vmjkvw6wk2zylhdklb577kgmihdms"))))
       ("jemalloc" ,jemalloc)
       ("libgff" ,libgff)
       ("tbb" ,tbb)
       ("libdivsufsort" ,libdivsufsort)
       ("libstadenio-for-salmon" ,libstadenio-for-salmon)
       ("xz" ,xz)
       ("zlib" ,zlib)))
    (native-inputs
     `(("pkg-config" ,pkg-config)))
    (home-page "https://github.com/COMBINE-lab/salmon")
    (synopsis "Quantification from RNA-seq reads using lightweight alignments")
    (description "Salmon is a program to produce highly-accurate,
transcript-level quantification estimates from RNA-seq data.  Salmon achieves
its accuracy and speed via a number of different innovations, including the
use of lightweight alignments (accurate but fast-to-compute proxies for
traditional read alignments) and massively-parallel stochastic collapsed
variational inference.")
    (license license:gpl3+)))

(define-public python-loompy
  (package
    (name "python-loompy")
    (version "2.0.17")
    ;; The tarball on Pypi does not include the tests.
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/linnarsson-lab/loompy")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "12a5kjgiikapv93wahfw0frszx1lblnppyz3vs5gy8fgmgngra07"))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (replace 'check
           (lambda _
             (setenv "PYTHONPATH"
                     (string-append (getcwd) ":"
                                    (getenv "PYTHONPATH")))
             (invoke "pytest" "tests")
             #t)))))
    (propagated-inputs
     `(("python-h5py" ,python-h5py)
       ("python-numpy" ,python-numpy)
       ("python-pandas" ,python-pandas)
       ("python-scipy" ,python-scipy)))
    (native-inputs
     `(("python-pytest" ,python-pytest)))
    (home-page "https://github.com/linnarsson-lab/loompy")
    (synopsis "Work with .loom files for single-cell RNA-seq data")
    (description "The loom file format is an efficient format for very large
omics datasets, consisting of a main matrix, optional additional layers, a
variable number of row and column annotations.  Loom also supports sparse
graphs.  This library makes it easy to work with @file{.loom} files for
single-cell RNA-seq data.")
    (license license:bsd-3)))

;; We cannot use the latest commit because it requires Java 9.
(define-public java-forester
  (let ((commit "86b07efe302d5094b42deed9260f719a4c4ac2e6")
        (revision "1"))
    (package
      (name "java-forester")
      (version (string-append "0-" revision "." (string-take commit 7)))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "https://github.com/cmzmasek/forester")
                      (commit commit)))
                (file-name (string-append name "-" version "-checkout"))
                (sha256
                 (base32
                  "0vxavc1yrf84yrnf20dq26hi0lglidk8d382xrxsy4qmlbjd276z"))
                (modules '((guix build utils)))
                (snippet
                 '(begin
                    ;; Delete bundled jars and pre-built classes
                    (delete-file-recursively "forester/java/resources")
                    (delete-file-recursively "forester/java/classes")
                    (for-each delete-file (find-files "forester/java/" "\\.jar$"))
                    ;; Delete bundled applications
                    (delete-file-recursively "forester_applications")
                    #t))))
      (build-system ant-build-system)
      (arguments
       `(#:tests? #f ; there are none
         #:jdk ,icedtea-8
         #:modules ((guix build ant-build-system)
                    (guix build utils)
                    (guix build java-utils)
                    (sxml simple)
                    (sxml transform))
         #:phases
         (modify-phases %standard-phases
           (add-after 'unpack 'chdir
             (lambda _ (chdir "forester/java") #t))
           (add-after 'chdir 'fix-dependencies
             (lambda _
               (chmod "build.xml" #o664)
               (call-with-output-file "build.xml.new"
                 (lambda (port)
                   (sxml->xml
                    (pre-post-order
                     (with-input-from-file "build.xml"
                       (lambda _ (xml->sxml #:trim-whitespace? #t)))
                     `(;; Remove all unjar tags to avoid repacking classes.
                       (unjar     . ,(lambda _ '()))
                       (*default* . ,(lambda (tag . kids) `(,tag ,@kids)))
                       (*text*    . ,(lambda (_ txt) txt))))
                    port)))
               (rename-file "build.xml.new" "build.xml")
               #t))
           ;; FIXME: itext is difficult to package as it depends on a few
           ;; unpackaged libraries.
           (add-after 'chdir 'remove-dependency-on-unpackaged-itext
             (lambda _
               (delete-file "src/org/forester/archaeopteryx/PdfExporter.java")
               (substitute* "src/org/forester/archaeopteryx/MainFrame.java"
                 (("pdf_written_to = PdfExporter.*")
                  "throw new IOException(\"PDF export is not available.\");"))
               #t))
           ;; There is no install target
           (replace 'install (install-jars ".")))))
      (propagated-inputs
       `(("java-commons-codec" ,java-commons-codec)
         ("java-openchart2" ,java-openchart2)))
      (home-page "https://sites.google.com/site/cmzmasek/home/software/forester")
      (synopsis "Phylogenomics libraries for Java")
      (description "Forester is a collection of Java libraries for
phylogenomics and evolutionary biology research.  It includes support for
reading, writing, and exporting phylogenetic trees.")
      (license license:lgpl2.1+))))

(define-public java-forester-1.005
  (package
    (name "java-forester")
    (version "1.005")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://repo1.maven.org/maven2/"
                                  "org/biojava/thirdparty/forester/"
                                  version "/forester-" version "-sources.jar"))
              (file-name (string-append name "-" version ".jar"))
              (sha256
               (base32
                "04r8qv4rk3p71z4ajrvp11py1z46qrx0047j3zzs79s6lnsm3lcv"))))
    (build-system ant-build-system)
    (arguments
     `(#:tests? #f ; there are none
       #:jdk ,icedtea-8
       #:modules ((guix build ant-build-system)
                  (guix build utils)
                  (guix build java-utils)
                  (sxml simple)
                  (sxml transform))
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'fix-dependencies
           (lambda* (#:key inputs #:allow-other-keys)
             (call-with-output-file "build.xml"
               (lambda (port)
                 (sxml->xml
                  (pre-post-order
                   (with-input-from-file "src/build.xml"
                     (lambda _ (xml->sxml #:trim-whitespace? #t)))
                   `(;; Remove all unjar tags to avoid repacking classes.
                     (unjar     . ,(lambda _ '()))
                     (*default* . ,(lambda (tag . kids) `(,tag ,@kids)))
                     (*text*    . ,(lambda (_ txt) txt))))
                  port)))
             (copy-file (assoc-ref inputs "synth_look_and_feel_1.xml")
                        "synth_look_and_feel_1.xml")
             (copy-file (assoc-ref inputs "phyloxml.xsd")
                        "phyloxml.xsd")
             (substitute* "build.xml"
               (("../resources/synth_laf/synth_look_and_feel_1.xml")
                "synth_look_and_feel_1.xml")
               (("../resources/phyloxml_schema/1.10/phyloxml.xsd")
                "phyloxml.xsd"))
             #t))
         ;; FIXME: itext is difficult to package as it depends on a few
         ;; unpackaged libraries.
         (add-after 'unpack 'remove-dependency-on-unpackaged-itext
           (lambda _
             (delete-file "src/org/forester/archaeopteryx/PdfExporter.java")
             (substitute* '("src/org/forester/archaeopteryx/MainFrame.java"
                            "src/org/forester/archaeopteryx/MainFrameApplication.java")
               (("pdf_written_to = PdfExporter.*")
                "throw new IOException(\"PDF export is not available.\"); /*")
               ((".getPrintSizeX\\(\\), getOptions\\(\\).getPrintSizeY\\(\\) \\);") "*/")
               (("getCurrentTreePanel\\(\\).getHeight\\(\\) \\);") "*/"))
             #t))
         (add-after 'unpack 'delete-pre-built-classes
           (lambda _ (delete-file-recursively "src/classes") #t))
         ;; There is no install target
         (replace 'install (install-jars ".")))))
    (propagated-inputs
     `(("java-commons-codec" ,java-commons-codec)
       ("java-openchart2" ,java-openchart2)))
    ;; The source archive does not contain the resources.
    (native-inputs
     `(("phyloxml.xsd"
        ,(origin
           (method url-fetch)
           (uri (string-append "https://raw.githubusercontent.com/cmzmasek/forester/"
                               "b61cc2dcede0bede317db362472333115756b8c6/"
                               "forester/resources/phyloxml_schema/1.10/phyloxml.xsd"))
           (file-name (string-append name "-phyloxml-" version ".xsd"))
           (sha256
            (base32
             "1zxc4m8sn4n389nqdnpxa8d0k17qnr3pm2y5y6g6vh4k0zm52npv"))))
       ("synth_look_and_feel_1.xml"
        ,(origin
           (method url-fetch)
           (uri (string-append "https://raw.githubusercontent.com/cmzmasek/forester/"
                               "29e04321615da6b35c1e15c60e52caf3f21d8e6a/"
                               "forester/java/classes/resources/"
                               "synth_look_and_feel_1.xml"))
           (file-name (string-append name "-synth-look-and-feel-" version ".xml"))
           (sha256
            (base32
             "1gv5602gv4k7y7713y75a4jvj7i9s7nildsbdl7n9q10sc2ikg8h"))))))
    (home-page "https://sites.google.com/site/cmzmasek/home/software/forester")
    (synopsis "Phylogenomics libraries for Java")
    (description "Forester is a collection of Java libraries for
phylogenomics and evolutionary biology research.  It includes support for
reading, writing, and exporting phylogenetic trees.")
    (license license:lgpl2.1+)))

(define-public java-biojava-core
  (package
    (name "java-biojava-core")
    (version "4.2.11")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/biojava/biojava")
                    (commit (string-append "biojava-" version))))
              (file-name (string-append name "-" version "-checkout"))
              (sha256
               (base32
                "1bvryh2bpsvash8ln79cmc9sqm8qw72hz4xzwqxcrjm8ssxszhqk"))))
    (build-system ant-build-system)
    (arguments
     `(#:jdk ,icedtea-8
       #:jar-name "biojava-core.jar"
       #:source-dir "biojava-core/src/main/java/"
       #:test-dir "biojava-core/src/test"
       ;; These tests seem to require internet access.
       #:test-exclude (list "**/SearchIOTest.java"
                            "**/BlastXMLParserTest.java"
                            "**/GenbankCookbookTest.java"
                            "**/GenbankProxySequenceReaderTest.java")
       #:phases
       (modify-phases %standard-phases
         (add-before 'build 'copy-resources
           (lambda _
             (copy-recursively "biojava-core/src/main/resources"
                               "build/classes")
             #t))
         (add-before 'check 'copy-test-resources
           (lambda _
             (copy-recursively "biojava-core/src/test/resources"
                               "build/test-classes")
             #t)))))
    (propagated-inputs
     `(("java-log4j-api" ,java-log4j-api)
       ("java-log4j-core" ,java-log4j-core)
       ("java-slf4j-api" ,java-slf4j-api)
       ("java-slf4j-simple" ,java-slf4j-simple)))
    (native-inputs
     `(("java-junit" ,java-junit)
       ("java-hamcrest-core" ,java-hamcrest-core)))
    (home-page "https://biojava.org")
    (synopsis "Core libraries of Java framework for processing biological data")
    (description "BioJava is a project dedicated to providing a Java framework
for processing biological data.  It provides analytical and statistical
routines, parsers for common file formats, reference implementations of
popular algorithms, and allows the manipulation of sequences and 3D
structures.  The goal of the biojava project is to facilitate rapid
application development for bioinformatics.

This package provides the core libraries.")
    (license license:lgpl2.1+)))

(define-public java-biojava-phylo
  (package (inherit java-biojava-core)
    (name "java-biojava-phylo")
    (build-system ant-build-system)
    (arguments
     `(#:jdk ,icedtea-8
       #:jar-name "biojava-phylo.jar"
       #:source-dir "biojava-phylo/src/main/java/"
       #:test-dir "biojava-phylo/src/test"
       #:phases
       (modify-phases %standard-phases
         (add-before 'build 'copy-resources
           (lambda _
             (copy-recursively "biojava-phylo/src/main/resources"
                               "build/classes")
             #t))
         (add-before 'check 'copy-test-resources
           (lambda _
             (copy-recursively "biojava-phylo/src/test/resources"
                               "build/test-classes")
             #t)))))
    (propagated-inputs
     `(("java-log4j-api" ,java-log4j-api)
       ("java-log4j-core" ,java-log4j-core)
       ("java-slf4j-api" ,java-slf4j-api)
       ("java-slf4j-simple" ,java-slf4j-simple)
       ("java-biojava-core" ,java-biojava-core)
       ("java-forester" ,java-forester)))
    (native-inputs
     `(("java-junit" ,java-junit)
       ("java-hamcrest-core" ,java-hamcrest-core)))
    (home-page "https://biojava.org")
    (synopsis "Biojava interface to the forester phylogenomics library")
    (description "The phylo module provides a biojava interface layer to the
forester phylogenomics library for constructing phylogenetic trees.")))

(define-public java-biojava-alignment
  (package (inherit java-biojava-core)
    (name "java-biojava-alignment")
    (build-system ant-build-system)
    (arguments
     `(#:jdk ,icedtea-8
       #:jar-name "biojava-alignment.jar"
       #:source-dir "biojava-alignment/src/main/java/"
       #:test-dir "biojava-alignment/src/test"
       #:phases
       (modify-phases %standard-phases
         (add-before 'build 'copy-resources
           (lambda _
             (copy-recursively "biojava-alignment/src/main/resources"
                               "build/classes")
             #t))
         (add-before 'check 'copy-test-resources
           (lambda _
             (copy-recursively "biojava-alignment/src/test/resources"
                               "build/test-classes")
             #t)))))
    (propagated-inputs
     `(("java-log4j-api" ,java-log4j-api)
       ("java-log4j-core" ,java-log4j-core)
       ("java-slf4j-api" ,java-slf4j-api)
       ("java-slf4j-simple" ,java-slf4j-simple)
       ("java-biojava-core" ,java-biojava-core)
       ("java-biojava-phylo" ,java-biojava-phylo)
       ("java-forester" ,java-forester)))
    (native-inputs
     `(("java-junit" ,java-junit)
       ("java-hamcrest-core" ,java-hamcrest-core)))
    (home-page "https://biojava.org")
    (synopsis "Biojava API for genetic sequence alignment")
    (description "The alignment module of BioJava provides an API that
contains

@itemize
@item implementations of dynamic programming algorithms for sequence
  alignment;
@item reading and writing of popular alignment file formats;
@item a single-, or multi- threaded multiple sequence alignment algorithm.
@end itemize\n")))

(define-public java-biojava-core-4.0
  (package (inherit java-biojava-core)
    (name "java-biojava-core")
    (version "4.0.0")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/biojava/biojava")
                    (commit (string-append "biojava-" version))))
              (file-name (string-append name "-" version "-checkout"))
              (sha256
               (base32
                "13675f6y9aqi7bi2lk3s1z7a22ynccjiqwa8izh7p97xi9wsfmd8"))))))

(define-public java-biojava-phylo-4.0
  (package (inherit java-biojava-core-4.0)
    (name "java-biojava-phylo")
    (build-system ant-build-system)
    (arguments
     `(#:jdk ,icedtea-8
       #:jar-name "biojava-phylo.jar"
       #:source-dir "biojava-phylo/src/main/java/"
       #:test-dir "biojava-phylo/src/test"
       #:phases
       (modify-phases %standard-phases
         (add-before 'build 'copy-resources
           (lambda _
             (copy-recursively "biojava-phylo/src/main/resources"
                               "build/classes")
             #t))
         (add-before 'check 'copy-test-resources
           (lambda _
             (copy-recursively "biojava-phylo/src/test/resources"
                               "build/test-classes")
             #t)))))
    (propagated-inputs
     `(("java-log4j-api" ,java-log4j-api)
       ("java-log4j-core" ,java-log4j-core)
       ("java-slf4j-api" ,java-slf4j-api)
       ("java-slf4j-simple" ,java-slf4j-simple)
       ("java-biojava-core" ,java-biojava-core-4.0)
       ("java-forester" ,java-forester-1.005)))
    (native-inputs
     `(("java-junit" ,java-junit)
       ("java-hamcrest-core" ,java-hamcrest-core)))
    (home-page "https://biojava.org")
    (synopsis "Biojava interface to the forester phylogenomics library")
    (description "The phylo module provides a biojava interface layer to the
forester phylogenomics library for constructing phylogenetic trees.")))

(define-public java-biojava-alignment-4.0
  (package (inherit java-biojava-core-4.0)
    (name "java-biojava-alignment")
    (build-system ant-build-system)
    (arguments
     `(#:jdk ,icedtea-8
       #:jar-name "biojava-alignment.jar"
       #:source-dir "biojava-alignment/src/main/java/"
       #:test-dir "biojava-alignment/src/test"
       #:phases
       (modify-phases %standard-phases
         (add-before 'build 'copy-resources
           (lambda _
             (copy-recursively "biojava-alignment/src/main/resources"
                               "build/classes")
             #t))
         (add-before 'check 'copy-test-resources
           (lambda _
             (copy-recursively "biojava-alignment/src/test/resources"
                               "build/test-classes")
             #t)))))
    (propagated-inputs
     `(("java-log4j-api" ,java-log4j-api)
       ("java-log4j-core" ,java-log4j-core)
       ("java-slf4j-api" ,java-slf4j-api)
       ("java-slf4j-simple" ,java-slf4j-simple)
       ("java-biojava-core" ,java-biojava-core-4.0)
       ("java-biojava-phylo" ,java-biojava-phylo-4.0)
       ("java-forester" ,java-forester-1.005)))
    (native-inputs
     `(("java-junit" ,java-junit)
       ("java-hamcrest-core" ,java-hamcrest-core)))
    (home-page "https://biojava.org")
    (synopsis "Biojava API for genetic sequence alignment")
    (description "The alignment module of BioJava provides an API that
contains

@itemize
@item implementations of dynamic programming algorithms for sequence
  alignment;
@item reading and writing of popular alignment file formats;
@item a single-, or multi- threaded multiple sequence alignment algorithm.
@end itemize\n")))

(define-public dropseq-tools
  (package
    (name "dropseq-tools")
    (version "1.13")
    (source
     (origin
       (method url-fetch)
       (uri "http://mccarrolllab.com/download/1276/")
       (file-name (string-append "dropseq-tools-" version ".zip"))
       (sha256
        (base32
         "0yrffckxqk5l8b5xb6z4laq157zd9mdypr2p4b4vq2bhjzi1sj0s"))
       ;; Delete bundled libraries
       (modules '((guix build utils)))
       (snippet
        '(begin
           (for-each delete-file (find-files "jar/lib" "\\.jar$"))
           (delete-file-recursively "3rdParty")
           #t))))
    (build-system ant-build-system)
    (arguments
     `(#:tests? #f                      ; test data are not included
       #:test-target "test"
       #:build-target "all"
       #:source-dir "public/src/"
       #:jdk ,icedtea-8
       #:make-flags
       (list (string-append "-Dpicard.executable.dir="
                            (assoc-ref %build-inputs "java-picard")
                            "/share/java/"))
       #:modules ((ice-9 match)
                  (srfi srfi-1)
                  (guix build utils)
                  (guix build java-utils)
                  (guix build ant-build-system))
       #:phases
       (modify-phases %standard-phases
         ;; FIXME: fails with "java.io.FileNotFoundException:
         ;; /gnu/store/…-dropseq-tools-1.13/share/java/lib/biojava-alignment.jar"
         (delete 'generate-jar-indices)
         ;; All dependencies must be linked to "lib", because that's where
         ;; they will be searched for when the Class-Path property of the
         ;; manifest is computed.
         (add-after 'unpack 'record-references
	   (lambda* (#:key inputs #:allow-other-keys)
             (mkdir-p "jar/lib")
             (let ((dirs (filter-map (match-lambda
                                       ((name . dir)
                                        (if (and (string-prefix? "java-" name)
                                                 (not (string=? name "java-testng")))
                                            dir #f)))
                                     inputs)))
               (for-each (lambda (jar)
                           (symlink jar (string-append "jar/lib/" (basename jar))))
                         (append-map (lambda (dir) (find-files dir "\\.jar$"))
                                     dirs)))
	     #t))
         ;; There is no installation target
         (replace 'install
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let* ((out     (assoc-ref outputs "out"))
                    (bin     (string-append out "/bin"))
                    (share   (string-append out "/share/java/"))
                    (lib     (string-append share "/lib/"))
                    (scripts (list "BAMTagHistogram"
                                   "BAMTagofTagCounts"
                                   "BaseDistributionAtReadPosition"
                                   "CollapseBarcodesInPlace"
                                   "CollapseTagWithContext"
                                   "ConvertToRefFlat"
                                   "CreateIntervalsFiles"
                                   "DetectBeadSynthesisErrors"
                                   "DigitalExpression"
                                   "Drop-seq_alignment.sh"
                                   "FilterBAM"
                                   "FilterBAMByTag"
                                   "GatherGeneGCLength"
                                   "GatherMolecularBarcodeDistributionByGene"
                                   "GatherReadQualityMetrics"
                                   "PolyATrimmer"
                                   "ReduceGTF"
                                   "SelectCellsByNumTranscripts"
                                   "SingleCellRnaSeqMetricsCollector"
                                   "TagBamWithReadSequenceExtended"
                                   "TagReadWithGeneExon"
                                   "TagReadWithInterval"
                                   "TrimStartingSequence"
                                   "ValidateReference")))
               (for-each mkdir-p (list bin share lib))
               (install-file "dist/dropseq.jar" share)
               (for-each (lambda (script)
                           (chmod script #o555)
                           (install-file script bin))
                         scripts)
               (substitute* (map (lambda (script)
                                   (string-append bin "/" script))
                                 scripts)
                 (("^java") (which "java"))
                 (("jar_deploy_dir=.*")
                  (string-append "jar_deploy_dir=" share "\n"))))
             #t))
         ;; FIXME: We do this after stripping jars because we don't want it to
         ;; copy all these jars and strip them.  We only want to install
         ;; links.  Arguably, this is a problem with the ant-build-system.
         (add-after 'strip-jar-timestamps 'install-links
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out     (assoc-ref outputs "out"))
                    (share   (string-append out "/share/java/"))
                    (lib     (string-append share "/lib/")))
               (for-each (lambda (jar)
                           (symlink (readlink jar)
                                    (string-append lib (basename jar))))
                         (find-files "jar/lib" "\\.jar$")))
             #t)))))
    (inputs
     `(("jdk" ,icedtea-8)
       ("java-picard" ,java-picard-2.10.3)
       ("java-log4j-1.2-api" ,java-log4j-1.2-api)
       ("java-commons-math3" ,java-commons-math3)
       ("java-commons-jexl2" ,java-commons-jexl-2)
       ("java-commons-collections4" ,java-commons-collections4)
       ("java-commons-lang2" ,java-commons-lang)
       ("java-commons-io" ,java-commons-io)
       ("java-snappy-1.0.3-rc3" ,java-snappy-1)
       ("java-guava" ,java-guava)
       ("java-la4j" ,java-la4j)
       ("java-biojava-core" ,java-biojava-core-4.0)
       ("java-biojava-alignment" ,java-biojava-alignment-4.0)
       ("java-jdistlib" ,java-jdistlib)
       ("java-simple-xml" ,java-simple-xml)
       ("java-snakeyaml" ,java-snakeyaml)))
    (native-inputs
     `(("unzip" ,unzip)
       ("java-testng" ,java-testng)))
    (home-page "http://mccarrolllab.com/dropseq/")
    (synopsis "Tools for Drop-seq analyses")
    (description "Drop-seq is a technology to enable biologists to
analyze RNA expression genome-wide in thousands of individual cells at
once.  This package provides tools to perform Drop-seq analyses.")
    (license license:expat)))

(define-public pigx-rnaseq
  (package
    (name "pigx-rnaseq")
    (version "0.0.10")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://github.com/BIMSBbioinfo/pigx_rnaseq/"
                                  "releases/download/v" version
                                  "/pigx_rnaseq-" version ".tar.gz"))
              (sha256
               (base32
                "0z3hr120wk2vrlmlpz1vp3n9wy3rq4y2mnzh2vf08qgqn2xfdwcw"))))
    (build-system gnu-build-system)
    (arguments
     `(#:parallel-tests? #f             ; not supported
       #:phases
       (modify-phases %standard-phases
         ;; "test.sh" runs STAR, which requires excessive amounts of memory.
         (add-after 'unpack 'disable-resource-intensive-test
           (lambda _
             (substitute* "Makefile.in"
               (("(^  tests/test_trim_galore/test.sh).*" _ m) m)
               (("^  tests/test_multiqc/test.sh") "")
               (("^  test.sh") ""))
             #t)))))
    (inputs
     `(("coreutils" ,coreutils)
       ("sed" ,sed)
       ("gzip" ,gzip)
       ("snakemake" ,snakemake)
       ("fastqc" ,fastqc)
       ("multiqc" ,multiqc)
       ("star" ,star)
       ("trim-galore" ,trim-galore)
       ("htseq" ,htseq)
       ("samtools" ,samtools)
       ("r-minimal" ,r-minimal)
       ("r-rmarkdown" ,r-rmarkdown)
       ("r-ggplot2" ,r-ggplot2)
       ("r-ggrepel" ,r-ggrepel)
       ("r-gprofiler" ,r-gprofiler)
       ("r-deseq2" ,r-deseq2)
       ("r-dt" ,r-dt)
       ("r-knitr" ,r-knitr)
       ("r-pheatmap" ,r-pheatmap)
       ("r-corrplot" ,r-corrplot)
       ("r-reshape2" ,r-reshape2)
       ("r-plotly" ,r-plotly)
       ("r-scales" ,r-scales)
       ("r-summarizedexperiment" ,r-summarizedexperiment)
       ("r-crosstalk" ,r-crosstalk)
       ("r-tximport" ,r-tximport)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-rjson" ,r-rjson)
       ("salmon" ,salmon)
       ("pandoc" ,pandoc)
       ("pandoc-citeproc" ,pandoc-citeproc)
       ("python-wrapper" ,python-wrapper)
       ("python-pyyaml" ,python-pyyaml)))
    (home-page "https://bioinformatics.mdc-berlin.de/pigx/")
    (synopsis "Analysis pipeline for RNA sequencing experiments")
    (description "PiGX RNAseq is an analysis pipeline for preprocessing and
reporting for RNA sequencing experiments.  It is easy to use and produces high
quality reports.  The inputs are reads files from the sequencing experiment,
and a configuration file which describes the experiment.  In addition to
quality control of the experiment, the pipeline produces a differential
expression report comparing samples in an easily configurable manner.")
    (license license:gpl3+)))

(define-public pigx-chipseq
  (package
    (name "pigx-chipseq")
    (version "0.0.43")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://github.com/BIMSBbioinfo/pigx_chipseq/"
                                  "releases/download/v" version
                                  "/pigx_chipseq-" version ".tar.gz"))
              (sha256
               (base32
                "0426i31b7mqqkbss5dgrvf5prkj4z1qrd7yrpd27vybs01xhdlks"))))
    (build-system gnu-build-system)
    ;; parts of the tests rely on access to the network
    (arguments '(#:tests? #f))
    (inputs
     `(("grep" ,grep)
       ("coreutils" ,coreutils)
       ("r-minimal" ,r-minimal)
       ("r-argparser" ,r-argparser)
       ("r-biocparallel" ,r-biocparallel)
       ("r-biostrings" ,r-biostrings)
       ("r-chipseq" ,r-chipseq)
       ("r-data-table" ,r-data-table)
       ("r-dplyr" ,r-dplyr)
       ("r-genomation" ,r-genomation)
       ("r-genomicalignments" ,r-genomicalignments)
       ("r-genomicranges" ,r-genomicranges)
       ("r-rsamtools" ,r-rsamtools)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-s4vectors" ,r-s4vectors)
       ("r-stringr" ,r-stringr)
       ("r-tibble" ,r-tibble)
       ("r-tidyr" ,r-tidyr)
       ("r-jsonlite" ,r-jsonlite)
       ("r-heatmaply" ,r-heatmaply)
       ("r-htmlwidgets" ,r-htmlwidgets)
       ("r-ggplot2" ,r-ggplot2)
       ("r-plotly" ,r-plotly)
       ("r-rmarkdown" ,r-rmarkdown)
       ("python-wrapper" ,python-wrapper)
       ("python-pyyaml" ,python-pyyaml)
       ("python-magic" ,python-magic)
       ("python-xlrd" ,python-xlrd)
       ("trim-galore" ,trim-galore)
       ("macs" ,macs)
       ("multiqc" ,multiqc)
       ("perl" ,perl)
       ("pandoc" ,pandoc)
       ("pandoc-citeproc" ,pandoc-citeproc)
       ("fastqc" ,fastqc)
       ("bowtie" ,bowtie)
       ("idr" ,idr)
       ("snakemake" ,snakemake)
       ("samtools" ,samtools)
       ("bedtools" ,bedtools)
       ("kentutils" ,kentutils)))
    (native-inputs
     `(("python-pytest" ,python-pytest)))
    (home-page "https://bioinformatics.mdc-berlin.de/pigx/")
    (synopsis "Analysis pipeline for ChIP sequencing experiments")
    (description "PiGX ChIPseq is an analysis pipeline for preprocessing, peak
calling and reporting for ChIP sequencing experiments.  It is easy to use and
produces high quality reports.  The inputs are reads files from the sequencing
experiment, and a configuration file which describes the experiment.  In
addition to quality control of the experiment, the pipeline enables to set up
multiple peak calling analysis and allows the generation of a UCSC track hub
in an easily configurable manner.")
    (license license:gpl3+)))

(define-public pigx-bsseq
  (package
    (name "pigx-bsseq")
    (version "0.1.2")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://github.com/BIMSBbioinfo/pigx_bsseq/"
                                  "releases/download/v" version
                                  "/pigx_bsseq-" version ".tar.gz"))
              (sha256
               (base32
                "0mpzlay2d5cjpmrcp7knff6rg1c2mqszd638n7lw0mc0cycbp9f8"))))
    (build-system gnu-build-system)
    (arguments
     `(;; TODO: tests currently require 12+GB of RAM.  See
       ;; https://github.com/BIMSBbioinfo/pigx_bsseq/issues/164
       #:tests? #f
       #:phases
       (modify-phases %standard-phases
         (add-before 'check 'set-timezone
           ;; The readr package is picky about timezones.
           (lambda* (#:key inputs #:allow-other-keys)
             (setenv "TZ" "UTC+1")
             (setenv "TZDIR"
                     (string-append (assoc-ref inputs "tzdata")
                                    "/share/zoneinfo"))
             #t)))))
    (native-inputs
     `(("tzdata" ,tzdata)))
    (inputs
     `(("coreutils" ,coreutils)
       ("sed" ,sed)
       ("grep" ,grep)
       ("r-minimal" ,r-minimal)
       ("r-annotationhub" ,r-annotationhub)
       ("r-dt" ,r-dt)
       ("r-genomation" ,r-genomation)
       ("r-ggrepel" ,r-ggrepel)
       ("r-methylkit" ,r-methylkit)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-rmarkdown" ,r-rmarkdown)
       ("r-bookdown" ,r-bookdown)
       ("r-ggplot2" ,r-ggplot2)
       ("r-ggbio" ,r-ggbio)
       ("pandoc" ,pandoc)
       ("pandoc-citeproc" ,pandoc-citeproc)
       ("python-wrapper" ,python-wrapper)
       ("python-pyyaml" ,python-pyyaml)
       ("snakemake" ,snakemake)
       ("bismark" ,bismark)
       ("bowtie" ,bowtie)
       ("bwa-meth" ,bwa-meth)
       ("fastqc" ,fastqc)
       ("methyldackel" ,methyldackel)
       ("multiqc" ,multiqc)
       ("trim-galore" ,trim-galore)
       ("cutadapt" ,cutadapt)
       ("samblaster" ,samblaster)
       ("samtools" ,samtools)))
    (home-page "https://bioinformatics.mdc-berlin.de/pigx/")
    (synopsis "Bisulfite sequencing pipeline from fastq to methylation reports")
    (description "PiGx BSseq is a data processing pipeline for raw fastq read
data of bisulfite experiments; it produces reports on aggregate methylation
and coverage and can be used to produce information on differential
methylation and segmentation.")
    (license license:gpl3+)))

(define-public pigx-scrnaseq
  (package
    (name "pigx-scrnaseq")
    (version "1.1.4")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://github.com/BIMSBbioinfo/pigx_scrnaseq/"
                                  "releases/download/v" version
                                  "/pigx_scrnaseq-" version ".tar.gz"))
              (sha256
               (base32
                "1d5l3gywypi67yz9advxq5xkgfhr4733gj0bwnngm723i3hdf5w9"))))
    (build-system gnu-build-system)
    (inputs
     `(("coreutils" ,coreutils)
       ("perl" ,perl)
       ("fastqc" ,fastqc)
       ("flexbar" ,flexbar)
       ("java" ,icedtea-8)
       ("jellyfish" ,jellyfish)
       ("python-wrapper" ,python-wrapper)
       ("python-pyyaml" ,python-pyyaml)
       ("python-pandas" ,python-pandas)
       ("python-magic" ,python-magic)
       ("python-numpy" ,python-numpy)
       ("python-loompy" ,python-loompy)
       ("pandoc" ,pandoc)
       ("pandoc-citeproc" ,pandoc-citeproc)
       ("samtools" ,samtools)
       ("snakemake" ,snakemake)
       ("star" ,star)
       ("r-minimal" ,r-minimal)
       ("r-argparser" ,r-argparser)
       ("r-cowplot" ,r-cowplot)
       ("r-data-table" ,r-data-table)
       ("r-delayedarray" ,r-delayedarray)
       ("r-delayedmatrixstats" ,r-delayedmatrixstats)
       ("r-dplyr" ,r-dplyr)
       ("r-dropbead" ,r-dropbead)
       ("r-dt" ,r-dt)
       ("r-genomicalignments" ,r-genomicalignments)
       ("r-genomicfiles" ,r-genomicfiles)
       ("r-genomicranges" ,r-genomicranges)
       ("r-ggplot2" ,r-ggplot2)
       ("r-hdf5array" ,r-hdf5array)
       ("r-pheatmap" ,r-pheatmap)
       ("r-rmarkdown" ,r-rmarkdown)
       ("r-rsamtools" ,r-rsamtools)
       ("r-rtracklayer" ,r-rtracklayer)
       ("r-rtsne" ,r-rtsne)
       ("r-scater" ,r-scater)
       ("r-scran" ,r-scran)
       ("r-seurat" ,r-seurat)
       ("r-singlecellexperiment" ,r-singlecellexperiment)
       ("r-stringr" ,r-stringr)
       ("r-yaml" ,r-yaml)))
    (home-page "https://bioinformatics.mdc-berlin.de/pigx/")
    (synopsis "Analysis pipeline for single-cell RNA sequencing experiments")
    (description "PiGX scRNAseq is an analysis pipeline for preprocessing and
quality control for single cell RNA sequencing experiments.  The inputs are
read files from the sequencing experiment, and a configuration file which
describes the experiment.  It produces processed files for downstream analysis
and interactive quality reports.  The pipeline is designed to work with UMI
based methods.")
    (license license:gpl3+)))

(define-public pigx
  (package
    (name "pigx")
    (version "0.0.3")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://github.com/BIMSBbioinfo/pigx/"
                                  "releases/download/v" version
                                  "/pigx-" version ".tar.gz"))
              (sha256
               (base32
                "1i5njdy1clj5ncw45d16p7mwmqvb1ilikl9n797pxklc3f4s7mq7"))))
    (build-system gnu-build-system)
    (inputs
     `(("python" ,python)
       ("pigx-bsseq" ,pigx-bsseq)
       ("pigx-chipseq" ,pigx-chipseq)
       ("pigx-rnaseq" ,pigx-rnaseq)
       ("pigx-scrnaseq" ,pigx-scrnaseq)))
    (home-page "https://bioinformatics.mdc-berlin.de/pigx/")
    (synopsis "Analysis pipelines for genomics")
    (description "PiGx is a collection of genomics pipelines.  It includes the
following pipelines:

@itemize
@item PiGx BSseq for raw fastq read data of bisulfite experiments
@item PiGx RNAseq for RNAseq samples
@item PiGx scRNAseq for single cell dropseq analysis
@item PiGx ChIPseq for reads from ChIPseq experiments
@end itemize

All pipelines are easily configured with a simple sample sheet and a
descriptive settings file.  The result is a set of comprehensive, interactive
HTML reports with interesting findings about your samples.")
    (license license:gpl3+)))

(define-public genrich
  (package
    (name "genrich")
    (version "0.5")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/jsh58/Genrich")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "0x0q6z0208n3cxzqjla4rgjqpyqgwpmz27852lcvzkzaigymq4zp"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; there are none
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (install-file "Genrich" (string-append (assoc-ref outputs "out") "/bin"))
             #t)))))
    (inputs
     `(("zlib" ,zlib)))
    (home-page "https://github.com/jsh58/Genrich")
    (synopsis "Detecting sites of genomic enrichment")
    (description "Genrich is a peak-caller for genomic enrichment
assays (e.g. ChIP-seq, ATAC-seq).  It analyzes alignment files generated
following the assay and produces a file detailing peaks of significant
enrichment.")
    (license license:expat)))

(define-public mantis
  (let ((commit "4ffd171632c2cb0056a86d709dfd2bf21bc69b84")
        (revision "1"))
    (package
      (name "mantis")
      (version (git-version "0" revision commit))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "https://github.com/splatlab/mantis")
                      (commit commit)))
                (file-name (git-file-name name version))
                (sha256
                 (base32
                  "0iqbr0dhmlc8mzpirmm2s4pkzkwdgrcx50yx6cv3wlr2qi064p55"))))
      (build-system cmake-build-system)
      (arguments '(#:tests? #f)) ; there are none
      (inputs
       `(("sdsl-lite" ,sdsl-lite)
         ("openssl" ,openssl)
         ("zlib" ,zlib)))
      (home-page "https://github.com/splatlab/mantis")
      (synopsis "Large-scale sequence-search index data structure")
      (description "Mantis is a space-efficient data structure that can be
used to index thousands of raw-read genomics experiments and facilitate
large-scale sequence searches on those experiments.  Mantis uses counting
quotient filters instead of Bloom filters, enabling rapid index builds and
queries, small indexes, and exact results, i.e., no false positives or
negatives.  Furthermore, Mantis is also a colored de Bruijn graph
representation, so it supports fast graph traversal and other topological
analyses in addition to large-scale sequence-level searches.")
      ;; uses __uint128_t and inline assembly
      (supported-systems '("x86_64-linux"))
      (license license:bsd-3))))

(define-public sjcount
  ;; There is no tag for version 3.2, nor is there a release archive.
  (let ((commit "292d3917cadb3f6834c81e509c30e61cd7ead6e5")
        (revision "1"))
    (package
      (name "sjcount")
      (version (git-version "3.2" revision commit))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "https://github.com/pervouchine/sjcount-full")
                      (commit commit)))
                (file-name (string-append name "-" version "-checkout"))
                (sha256
                 (base32
                  "0gdgj35j249f04rqgq8ymcc1xg1vi9kzbajnjqpaq2wpbh8bl234"))))
      (build-system gnu-build-system)
      (arguments
       `(#:tests? #f                    ; requires a 1.4G test file
         #:make-flags
         (list (string-append "SAMTOOLS_DIR="
                              (assoc-ref %build-inputs "samtools")
                              "/lib/"))
         #:phases
         (modify-phases %standard-phases
           (replace 'configure
             (lambda* (#:key inputs #:allow-other-keys)
               (substitute* "makefile"
                 (("-I \\$\\{SAMTOOLS_DIR\\}")
                  (string-append "-I" (assoc-ref inputs "samtools")
                                 "/include/samtools"))
                 (("-lz ") "-lz -lpthread "))
               #t))
           (replace 'install
             (lambda* (#:key outputs #:allow-other-keys)
               (for-each (lambda (tool)
                           (install-file tool
                                         (string-append (assoc-ref outputs "out")
                                                        "/bin")))
                         '("j_count" "b_count" "sjcount"))
               #t)))))
      (inputs
       `(("samtools" ,samtools-0.1)
         ("zlib" ,zlib)))
      (home-page "https://github.com/pervouchine/sjcount-full/")
      (synopsis "Annotation-agnostic splice junction counting pipeline")
      (description "Sjcount is a utility for fast quantification of splice
junctions in RNA-seq data.  It is annotation-agnostic and offset-aware.  This
version does count multisplits.")
      (license license:gpl3+))))

(define-public minimap2
  (package
    (name "minimap2")
    (version "2.17")
    (source
     (origin
       (method url-fetch)
       (uri (string-append "https://github.com/lh3/minimap2/"
                           "releases/download/v" version "/"
                           "minimap2-" version ".tar.bz2"))
       (sha256
        (base32
         "0hi7i9pzxhvjj44khzzzj1lrn5gb5837arr4wgln7k1k5n4ci2mn"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f                      ; there are none
       #:make-flags
       (list "CC=gcc"
             (let ((system ,(or (%current-target-system)
                                (%current-system))))
               (cond
                ((string-prefix? "x86_64" system)
                 "all")
                ((or (string-prefix? "armhf" system)
                     (string-prefix? "aarch64" system))
                 "arm_neon=1")
                (else "sse2only=1"))))
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin"))
                    (man (string-append out "/share/man/man1")))
               (install-file "minimap2" bin)
               (mkdir-p man)
               (install-file "minimap2.1" man))
             #t)))))
    (inputs
     `(("zlib" ,zlib)))
    (home-page "https://lh3.github.io/minimap2/")
    (synopsis "Pairwise aligner for genomic and spliced nucleotide sequences")
    (description "Minimap2 is a versatile sequence alignment program that
aligns DNA or mRNA sequences against a large reference database.  Typical use
cases include:

@enumerate
@item mapping PacBio or Oxford Nanopore genomic reads to the human genome;
@item finding overlaps between long reads with error rate up to ~15%;
@item splice-aware alignment of PacBio Iso-Seq or Nanopore cDNA or Direct RNA
  reads against a reference genome;
@item aligning Illumina single- or paired-end reads;
@item assembly-to-assembly alignment;
@item full-genome alignment between two closely related species with
  divergence below ~15%.
@end enumerate\n")
    (license license:expat)))

(define-public miniasm
  (package
   (name "miniasm")
   (version "0.3")
   (source (origin
            (method git-fetch)
            (uri (git-reference
                   (url "https://github.com/lh3/miniasm")
                   (commit (string-append "v" version))))
            (file-name (git-file-name name version))
            (sha256
             (base32
              "04dv5wv8bhsw1imxwyd438bnn9kby7svp44nbcz8lsadzjjci5gs"))))
   (build-system gnu-build-system)
   (inputs
    `(("zlib" ,zlib)))
   (arguments
    `(#:tests? #f ; There are no tests.
      #:phases
      (modify-phases %standard-phases
        (delete 'configure)
        (replace 'install
          (lambda* (#:key inputs outputs #:allow-other-keys)
            (let ((bin (string-append (assoc-ref outputs "out") "/bin")))
              (install-file "miniasm" bin)
              (install-file "minidot" bin)
              #t))))))
   (home-page "https://github.com/lh3/miniasm")
   (synopsis "Ultrafast de novo assembly for long noisy reads")
   (description "Miniasm is a very fast OLC-based de novo assembler for noisy
long reads.  It takes all-vs-all read self-mappings (typically by minimap) as
input and outputs an assembly graph in the GFA format.  Different from
mainstream assemblers, miniasm does not have a consensus step.  It simply
concatenates pieces of read sequences to generate the final unitig sequences.
Thus the per-base error rate is similar to the raw input reads.")
   (license license:expat)))

(define-public r-circus
  (package
    (name "r-circus")
    (version "0.1.5")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/BIMSBbioinfo/ciRcus")
             (commit (string-append "v" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "0jhjn3ilb057hbf6yzrihj13ifxxs32y7nkby8l3lkm28dg4p97h"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-annotationdbi" ,r-annotationdbi)
       ("r-annotationhub" ,r-annotationhub)
       ("r-biomart" ,r-biomart)
       ("r-data-table" ,r-data-table)
       ("r-dbi" ,r-dbi)
       ("r-genomicfeatures" ,r-genomicfeatures)
       ("r-genomicranges" ,r-genomicranges)
       ("r-ggplot2" ,r-ggplot2)
       ("r-hash" ,r-hash)
       ("r-iranges" ,r-iranges)
       ("r-rcolorbrewer" ,r-rcolorbrewer)
       ("r-rmysql" ,r-rmysql)
       ("r-s4vectors" ,r-s4vectors)
       ("r-stringr" ,r-stringr)
       ("r-summarizedexperiment" ,r-summarizedexperiment)))
    (native-inputs
     `(("r-knitr" ,r-knitr)))
    (home-page "https://github.com/BIMSBbioinfo/ciRcus")
    (synopsis "Annotation, analysis and visualization of circRNA data")
    (description "Circus is an R package for annotation, analysis and
visualization of circRNA data.  Users can annotate their circRNA candidates
with host genes, gene featrues they are spliced from, and discriminate between
known and yet unknown splice junctions.  Circular-to-linear ratios of circRNAs
can be calculated, and a number of descriptive plots easily generated.")
    (license license:artistic2.0)))

(define-public gffread
  ;; We cannot use the tagged release because it is not in sync with gclib.
  ;; See https://github.com/gpertea/gffread/issues/26
  (let ((commit "ba7535fcb3cea55a6e5a491d916e93b454e87fd0")
        (revision "1"))
    (package
      (name "gffread")
      (version (git-version "0.9.12" revision commit))
      (source
       (origin
         (method git-fetch)
         (uri (git-reference
               (url "https://github.com/gpertea/gffread")
               (commit commit)))
         (file-name (git-file-name name version))
         (sha256
          (base32
           "1dl2nbcg96lxpd0drg48ssa8343nf7pw9s9mkrc4mjjmfwsin3ki"))))
      (build-system gnu-build-system)
      (arguments
       `(#:tests? #f ; no check target
         #:make-flags
         (list "GCLDIR=gclib")
         #:phases
         (modify-phases %standard-phases
           (delete 'configure)
           (add-after 'unpack 'copy-gclib-source
             (lambda* (#:key inputs #:allow-other-keys)
               (mkdir-p "gclib")
               (copy-recursively (assoc-ref inputs "gclib-source") "gclib")
               #t))
           ;; There is no install target
           (replace 'install
             (lambda* (#:key outputs #:allow-other-keys)
               (let* ((out (assoc-ref outputs "out"))
                      (bin (string-append out "/bin")))
                 (install-file "gffread" bin))
               #t)))))
      (native-inputs
       `(("gclib-source"
          ,(let ((version "0.10.3")
                 (commit "54917d0849c1e83cfb057b5f712e5cb6a35d948f")
                 (revision "1"))
             (origin
               (method git-fetch)
               (uri (git-reference
                     (url "https://github.com/gpertea/gclib")
                     (commit commit)))
               (file-name (git-file-name "gclib" version))
               (sha256
                (base32
                 "0b51lc0b8syrv7186fd7n8f15rwnf264qgfmm2palrwks1px24mr")))))))
      (home-page "https://github.com/gpertea/gffread/")
      (synopsis "Parse and convert GFF/GTF files")
      (description
       "This package provides a GFF/GTF file parsing utility providing format
conversions, region filtering, FASTA sequence extraction and more.")
      ;; gffread is under Expat, but gclib is under Artistic 2.0
      (license (list license:expat
                     license:artistic2.0)))))

(define-public find-circ
  ;; The last release was in 2015.  The license was clarified in 2017, so we
  ;; take the latest commit.
  (let ((commit "8655dca54970fcf7e92e22fbf57e1188724dda7d")
        (revision "1"))
    (package
      (name "find-circ")
      (version (git-version "1.2" revision commit))
      (source
       (origin
         (method git-fetch)
         (uri (git-reference
               (url "https://github.com/marvin-jens/find_circ")
               (commit commit)))
         (file-name (git-file-name name version))
         (sha256
          (base32
           "0p77pbqbclqr4srms34y1b9b4njybfpjiknc11ki84f3p8skb3cg"))))
      (build-system gnu-build-system)
      (arguments
       `(#:tests? #f                    ; there are none
         #:phases
         ;; There is no actual build system.
         (modify-phases %standard-phases
           (delete 'configure)
           (delete 'build)
           (replace 'install
             (lambda* (#:key outputs #:allow-other-keys)
               (let* ((out (assoc-ref outputs "out"))
                      (bin (string-append out "/bin"))
                      (path (getenv "PYTHONPATH")))
                 (for-each (lambda (script)
                             (install-file script bin)
                             (wrap-program (string-append bin "/" script)
                               `("PYTHONPATH" ":" prefix (,path))))
                           '("cmp_bed.py"
                             "find_circ.py"
                             "maxlength.py"
                             "merge_bed.py"
                             "unmapped2anchors.py")))
               #t)))))
      (inputs
       `(("python2" ,python-2)
         ("python2-pysam" ,python2-pysam)
         ("python2-numpy" ,python2-numpy)))
      (home-page "https://github.com/marvin-jens/find_circ")
      (synopsis "circRNA detection from RNA-seq reads")
      (description "This package provides tools to detect head-to-tail
spliced (back-spliced) sequencing reads, indicative of circular RNA (circRNA)
in RNA-seq data.")
      (license license:gpl3))))

(define-public python-scanpy
  (package
    (name "python-scanpy")
    (version "1.4.6")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "scanpy" version))
       (sha256
        (base32
         "0s2b6cvaigx4wzw3850qb93sjwwxbzh22kpbp498zklc5rjpbz4l"))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (replace 'check
           (lambda* (#:key inputs #:allow-other-keys)
             ;; These tests require Internet access.
             (delete-file-recursively "scanpy/tests/notebooks")
             (delete-file "scanpy/tests/test_clustering.py")
             (delete-file "scanpy/tests/test_datasets.py")

             ;; TODO: I can't get the plotting tests to work, even with Xvfb.
             (delete-file "scanpy/tests/test_plotting.py")
             (delete-file "scanpy/tests/test_preprocessing.py")
             (delete-file "scanpy/tests/test_read_10x.py")

             (setenv "PYTHONPATH"
                     (string-append (getcwd) ":"
                                    (getenv "PYTHONPATH")))
             (invoke "pytest")
             #t)))))
    (propagated-inputs
     `(("python-anndata" ,python-anndata)
       ("python-h5py" ,python-h5py)
       ("python-igraph" ,python-igraph)
       ("python-joblib" ,python-joblib)
       ("python-legacy-api-wrap" ,python-legacy-api-wrap)
       ("python-louvain" ,python-louvain)
       ("python-matplotlib" ,python-matplotlib)
       ("python-natsort" ,python-natsort)
       ("python-networkx" ,python-networkx)
       ("python-numba" ,python-numba)
       ("python-packaging" ,python-packaging)
       ("python-pandas" ,python-pandas)
       ("python-patsy" ,python-patsy)
       ("python-scikit-learn" ,python-scikit-learn)
       ("python-scipy" ,python-scipy)
       ("python-seaborn" ,python-seaborn)
       ("python-statsmodels" ,python-statsmodels)
       ("python-tables" ,python-tables)
       ("python-tqdm" ,python-tqdm)
       ("python-umap-learn" ,python-umap-learn)))
    (native-inputs
     `(("python-pytest" ,python-pytest)
       ("python-setuptools-scm" ,python-setuptools-scm)))
    (home-page "https://github.com/theislab/scanpy")
    (synopsis "Single-Cell Analysis in Python.")
    (description "Scanpy is a scalable toolkit for analyzing single-cell gene
expression data.  It includes preprocessing, visualization, clustering,
pseudotime and trajectory inference and differential expression testing.  The
Python-based implementation efficiently deals with datasets of more than one
million cells.")
    (license license:bsd-3)))

(define-public python-bbknn
  (package
    (name "python-bbknn")
    (version "1.3.6")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "bbknn" version))
       (sha256
        (base32
         "1jbsh01f57zj4bhvjr3jh4532zznqd6nccmgrl3qi9gnhkf7c4y0"))))
    (build-system python-build-system)
    (arguments
     `(#:tests? #f)) ; TODO: Enable after migration to scikit-learn.
    (propagated-inputs
     `(("python-annoy" ,python-annoy)
       ("python-cython" ,python-cython)
       ("python-numpy" ,python-numpy)
       ("python-scipy" ,python-scipy)
       ("python-umap-learn" ,python-umap-learn)))
    (home-page "https://github.com/Teichlab/bbknn")
    (synopsis "Batch balanced KNN")
    (description "BBKNN is a batch effect removal tool that can be directly
used in the Scanpy workflow.  It serves as an alternative to
@code{scanpy.api.pp.neighbors()}, with both functions creating a neighbour
graph for subsequent use in clustering, pseudotime and UMAP visualisation.  If
technical artifacts are present in the data, they will make it challenging to
link corresponding cell types across different batches.  BBKNN actively
combats this effect by splitting your data into batches and finding a smaller
number of neighbours for each cell within each of the groups.  This helps
create connections between analogous cells in different batches without
altering the counts or PCA space.")
    (license license:expat)))

(define-public gffcompare
  (let ((commit "be56ef4349ea3966c12c6397f85e49e047361c41")
        (revision "1"))
    (package
      (name "gffcompare")
      (version (git-version "0.10.15" revision commit))
      (source
       (origin
         (method git-fetch)
         (uri (git-reference
               (url "https://github.com/gpertea/gffcompare/")
               (commit commit)))
         (file-name (git-file-name name version))
         (sha256
          (base32 "0cp5qpxdhw4mxpya5dld8wi3jk00zyklm6rcri426wydinrnfmkg"))))
      (build-system gnu-build-system)
      (arguments
       `(#:tests? #f                    ; no check target
         #:phases
         (modify-phases %standard-phases
           (delete 'configure)
           (add-before 'build 'copy-gclib-source
             (lambda* (#:key inputs #:allow-other-keys)
               (mkdir "../gclib")
               (copy-recursively
                (assoc-ref inputs "gclib-source") "../gclib")
               #t))
           (replace 'install
             (lambda* (#:key outputs #:allow-other-keys)
               (let ((bin (string-append (assoc-ref outputs "out") "/bin")))
                 (install-file "gffcompare" bin)
                 #t))))))
      (native-inputs
       `(("gclib-source" ; see 'README.md' of gffcompare
          ,(let ((commit "54917d0849c1e83cfb057b5f712e5cb6a35d948f")
                 (revision "1")
                 (name "gclib")
                 (version (git-version "0.10.3" revision commit)))
             (origin
               (method git-fetch)
               (uri (git-reference
                     (url "https://github.com/gpertea/gclib/")
                     (commit commit)))
               (file-name (git-file-name name version))
               (sha256
                (base32 "0b51lc0b8syrv7186fd7n8f15rwnf264qgfmm2palrwks1px24mr")))))))
      (home-page "https://github.com/gpertea/gffcompare/")
      (synopsis "Tool for comparing or classifing transcripts of RNA-Seq")
      (description
       "@code{gffcompare} is a tool that can:
@enumerate
@item compare and evaluate the accuracy of RNA-Seq transcript assemblers
(Cufflinks, Stringtie);
@item collapse (merge) duplicate transcripts from multiple GTF/GFF3 files (e.g.
resulted from assembly of different samples);
@item classify transcripts from one or multiple GTF/GFF3 files as they relate to
reference transcripts provided in a annotation file (also in GTF/GFF3 format).
@end enumerate")
      (license
       (list
        license:expat                   ;license for gffcompare
        license:artistic2.0)))))        ;license for gclib

(define-public intervaltree
  (let ((commit "b90527f9e6d51cd36ecbb50429e4524d3a418ea5"))
    (package
      (name "intervaltree")
      (version (git-version "0.0.0" "1" commit))
      (source
        (origin
          (method git-fetch)
          (uri (git-reference
                 (url "https://github.com/ekg/intervaltree/")
                 (commit commit)))
          (file-name (git-file-name name version))
          (sha256
           (base32 "0rgv6q5fl4x5d74n6p5wvdna6zmbdbqpb4jqqh6vq3670gn08xad"))))
      (build-system gnu-build-system)
      (arguments
       '(#:tests? #f ; No tests.
         #:make-flags (list (string-append "PREFIX=" (assoc-ref %outputs "out"))
                            "DESTDIR=\"\"")
         #:phases
         (modify-phases %standard-phases
           (delete 'configure)))) ; There is no configure phase.
      (home-page "https://github.com/ekg/intervaltree")
      (synopsis "Minimal C++ interval tree implementation")
      (description "An interval tree can be used to efficiently find a set of
numeric intervals overlapping or containing another interval.  This library
provides a basic implementation of an interval tree using C++ templates,
allowing the insertion of arbitrary types into the tree.")
      (license license:expat))))

(define-public python-intervaltree
  (package
    (name "python-intervaltree")
    (version "3.0.2")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "intervaltree" version))
       (sha256
        (base32
         "0wz234g6irlm4hivs2qzmnywk0ss06ckagwh15nflkyb3p462kyb"))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         ;; pytest seems to have a check to make sure the user is testing
         ;; their checked-out code and not an installed, potentially
         ;; out-of-date copy. This is harmless here, since we just installed
         ;; the package, so we disable the check to avoid skipping tests
         ;; entirely.
         (add-before 'check 'import-mismatch-error-workaround
           (lambda _
             (setenv "PY_IGNORE_IMPORTMISMATCH" "1")
             #t)))))
    (propagated-inputs
     `(("python-sortedcontainers" ,python-sortedcontainers)))
    (native-inputs
     `(("python-pytest" ,python-pytest)))
    (home-page "https://github.com/chaimleib/intervaltree")
    (synopsis "Editable interval tree data structure")
    (description
     "This package provides a mutable, self-balancing interval tree
implementation for Python.  Queries may be by point, by range overlap, or by
range envelopment.  This library was designed to allow tagging text and time
intervals, where the intervals include the lower bound but not the upper
bound.")
    (license license:asl2.0)))

(define-public python-pypairix
  (package
    (name "python-pypairix")
    (version "0.3.7")
    ;; The tarball on pypi does not include the makefile to build the
    ;; programs.
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/4dn-dcic/pairix")
             (commit version)))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "1snr3lrmsld8sy77ng6ba6wcmd33xjccf1l2f3m6pi29xis9nd6p"))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-before 'build 'build-programs
           (lambda _ (invoke "make")))
         (add-after 'install 'install-programs
           (lambda* (#:key outputs #:allow-other-keys)
             (copy-recursively "bin" (string-append
                                      (assoc-ref outputs "out")
                                      "/bin"))
             #t)))))
    (inputs
     `(("zlib" ,zlib)))
    (home-page "https://github.com/4dn-dcic/pairix")
    (synopsis "Support for querying pairix-indexed bgzipped text files")
    (description
     "Pypairix is a Python module for fast querying on a pairix-indexed
bgzipped text file that contains a pair of genomic coordinates per line.")
    (license license:expat)))

(define-public python-pyfaidx
  (package
    (name "python-pyfaidx")
    (version "0.5.8")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "pyfaidx" version))
       (sha256
        (base32
         "038xi3a6zvrxbyyfpp64ka8pcjgsdq4fgw9cl5lpxbvmm1bzzw2q"))))
    (build-system python-build-system)
    (propagated-inputs
     `(("python-six" ,python-six)))
    (home-page "http://mattshirley.com")
    (synopsis "Random access to fasta subsequences")
    (description
     "This package provides procedures for efficient pythonic random access to
fasta subsequences.")
    (license license:bsd-3)))

(define-public python2-pyfaidx
  (package-with-python2 python-pyfaidx))

(define-public python-cooler
  (package
    (name "python-cooler")
    (version "0.8.7")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "cooler" version))
       (sha256
        (base32
         "01g6gqix9ba27sappz6nfyiwabzrlf8i5fn8kwcz8ra356cq9crp"))))
    (build-system python-build-system)
    (propagated-inputs
     `(("python-asciitree" ,python-asciitree)
       ("python-biopython" ,python-biopython)
       ("python-click" ,python-click)
       ("python-cytoolz" ,python-cytoolz)
       ("python-dask" ,python-dask)
       ("python-h5py" ,python-h5py)
       ("python-multiprocess" ,python-multiprocess)
       ("python-numpy" ,python-numpy)
       ("python-pandas" ,python-pandas)
       ("python-pyfaidx" ,python-pyfaidx)
       ("python-pypairix" ,python-pypairix)
       ("python-pysam" ,python-pysam)
       ("python-pyyaml" ,python-pyyaml)
       ("python-scipy" ,python-scipy)
       ("python-simplejson" ,python-simplejson)))
    (native-inputs
     `(("python-mock" ,python-mock)
       ("python-pytest" ,python-pytest)))
    (home-page "https://github.com/mirnylab/cooler")
    (synopsis "Sparse binary format for genomic interaction matrices")
    (description
     "Cooler is a support library for a sparse, compressed, binary persistent
storage format, called @code{cool}, used to store genomic interaction data,
such as Hi-C contact matrices.")
    (license license:bsd-3)))

(define-public python-hicmatrix
  (package
    (name "python-hicmatrix")
    (version "12")
    (source
     (origin
       ;; Version 12 is not available on pypi.
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/deeptools/HiCMatrix")
             (commit version)))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "1xhdyx16f3brgxgxybixdi64ki8nbbkq5vk4h9ahi11pzpjfn1pj"))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'relax-requirements
           (lambda _
             (substitute* '("requirements.txt"
                            "setup.py")
               (("cooler *=+ *0.8.5")
                "cooler==0.8.*"))
             #t)))))
    (propagated-inputs
     `(("python-cooler" ,python-cooler)
       ("python-intervaltree" ,python-intervaltree)
       ("python-numpy" ,python-numpy)
       ("python-pandas" ,python-pandas)
       ("python-scipy" ,python-scipy)
       ("python-tables" ,python-tables)))
    (home-page "https://github.com/deeptools/HiCMatrix/")
    (synopsis "HiCMatrix class for HiCExplorer and pyGenomeTracks")
    (description
     "This helper package implements the @code{HiCMatrix} class for
the HiCExplorer and pyGenomeTracks packages.")
    (license license:gpl3+)))

(define-public python-hicexplorer
  (package
    (name "python-hicexplorer")
    (version "2.1.4")
    (source
     (origin
       ;; The latest version is not available on Pypi.
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/deeptools/HiCExplorer")
             (commit version)))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "0q5gpbzmrkvygqgw524q36b4nrivcmyi5v194vsx0qw7b3gcmq08"))))
    (build-system python-build-system)
    (arguments
     `(#:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'loosen-up-requirements
           (lambda _
             (substitute* "setup.py"
               (("==") ">="))
             #t)))))
    (propagated-inputs
     `(("python-biopython" ,python-biopython)
       ("python-configparser" ,python-configparser)
       ("python-cooler" ,python-cooler)
       ("python-future" ,python-future)
       ("python-intervaltree" ,python-intervaltree)
       ("python-jinja2" ,python-jinja2)
       ("python-matplotlib" ,python-matplotlib)
       ("python-numpy" ,python-numpy)
       ("python-pandas" ,python-pandas)
       ("python-pybigwig" ,python-pybigwig)
       ("python-pysam" ,python-pysam)
       ("python-scipy" ,python-scipy)
       ("python-six" ,python-six)
       ("python-tables" ,python-tables)
       ("python-unidecode" ,python-unidecode)))
    (home-page "https://hicexplorer.readthedocs.io")
    (synopsis "Process, analyze and visualize Hi-C data")
    (description
     "HiCExplorer is a powerful and easy to use set of tools to process,
normalize and visualize Hi-C data.  HiCExplorer facilitates the creation of
contact matrices, correction of contacts, TAD detection, A/B compartments,
merging, reordering or chromosomes, conversion from different formats
including cooler and detection of long-range contacts.  Moreover, it allows
the visualization of multiple contact matrices along with other types of data
like genes, compartments, ChIP-seq coverage tracks (and in general any type of
genomic scores), long range contacts and the visualization of viewpoints.")
    (license license:gpl3)))

(define-public python-pygenometracks
  (package
    (name "python-pygenometracks")
    (version "3.3")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "pyGenomeTracks" version))
       (sha256
        (base32
         "16laa0wnf4qn9fb9ych4w1vqhqwjss70v0y0f6wp4gwqfrlgac0f"))))
    (build-system python-build-system)
    (arguments
     `(#:tests? #f ; there are none
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'relax-requirements
           (lambda _
             (substitute* "setup.py"
               (("matplotlib ==3.1.1")
                "matplotlib >=3.1.1"))
             #t)))))
    (propagated-inputs
     `(("python-future" ,python-future)
       ("python-gffutils" ,python-gffutils)
       ("python-hicmatrix" ,python-hicmatrix)
       ("python-intervaltree" ,python-intervaltree)
       ("python-matplotlib" ,python-matplotlib)
       ("python-numpy" ,python-numpy)
       ("python-pybigwig" ,python-pybigwig)
       ("python-pysam" ,python-pysam)
       ("python-tqdm" ,python-tqdm)))
    (native-inputs
     `(("python-pytest" ,python-pytest)))
    (home-page "https://pygenometracks.readthedocs.io")
    (synopsis "Program and library to plot beautiful genome browser tracks")
    (description
     "This package aims to produce high-quality genome browser tracks that
are highly customizable.  Currently, it is possible to plot: bigwig, bed (many
options), bedgraph, links (represented as arcs), and Hi-C matrices.
pyGenomeTracks can make plots with or without Hi-C data.")
    (license license:gpl3+)))

(define-public python-hic2cool
  (package
    (name "python-hic2cool")
    (version "0.4.2")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "hic2cool" version))
       (sha256
        (base32
         "0xy6mhfns2lzib1kcr6419jjp6pmh0qx8z8na55lmiwn0ds8q9cl"))))
    (build-system python-build-system)
    (arguments '(#:tests? #f)) ; no tests included
    (propagated-inputs
     `(("python-cooler" ,python-cooler)))
    (home-page "https://github.com/4dn-dcic/hic2cool")
    (synopsis "Converter for .hic and .cool files")
    (description
     "This package provides a converter between @code{.hic} files (from
juicer) and single-resolution or multi-resolution @code{.cool} files (for
cooler).  Both @code{hic} and @code{cool} files describe Hi-C contact
matrices.")
    (license license:expat)))

(define-public r-pore
  (package
    (name "r-pore")
    (version "0.24")
    (source
     (origin
       (method url-fetch)
       (uri
        (string-append "mirror://sourceforge/rpore/" version
                       "/poRe_" version ".tar.gz"))
       (sha256
        (base32 "0pih9nljbv8g4x8rkk29i7aqq681b782r5s5ynp4nw9yzqnmmksv"))))
    (properties `((upstream-name . "poRe")))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-bit64" ,r-bit64)
       ("r-data-table" ,r-data-table)
       ("r-rhdf5" ,r-rhdf5)
       ("r-shiny" ,r-shiny)
       ("r-svdialogs" ,r-svdialogs)))
    (home-page "https://sourceforge.net/projects/rpore/")
    (synopsis "Visualize Nanopore sequencing data")
    (description
     "This package provides graphical user interfaces to organize and visualize Nanopore
sequencing data.")
    ;; This is free software but the license variant is unclear:
    ;; <https://github.com/mw55309/poRe_docs/issues/10>.
    (license license:bsd-3)))

(define-public r-xbioc
  (let ((revision "1")
        (commit "6ff0670a37ab3036aaf1d94aa4b208310946b0b5"))
    (package
      (name "r-xbioc")
      (version (git-version "0.1.16" revision commit))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "https://github.com/renozao/xbioc")
                      (commit commit)))
                (file-name (git-file-name name version))
                (sha256
                 (base32
                  "0w8bsq5myiwkfhh83nm6is5ichiyvwa1axx2szvxnzq39x6knf66"))))
      (build-system r-build-system)
      (propagated-inputs
       `(("r-annotationdbi" ,r-annotationdbi)
         ("r-assertthat" ,r-assertthat)
         ("r-biobase" ,r-biobase)
         ("r-biocmanager" ,r-biocmanager)
         ("r-digest" ,r-digest)
         ("r-pkgmaker" ,r-pkgmaker)
         ("r-plyr" ,r-plyr)
         ("r-reshape2" ,r-reshape2)
         ("r-stringr" ,r-stringr)))
      (home-page "https://github.com/renozao/xbioc/")
      (synopsis "Extra base functions for Bioconductor")
      (description "This package provides extra utility functions to perform
common tasks in the analysis of omics data, leveraging and enhancing features
provided by Bioconductor packages.")
      (license license:gpl3+))))

(define-public r-cssam
  (let ((revision "1")
        (commit "9ec58c982fa551af0d80b1a266890d92954833f2"))
    (package
      (name "r-cssam")
      (version (git-version "1.4" revision commit))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "https://github.com/shenorrLab/csSAM")
                      (commit commit)))
                (file-name (git-file-name name version))
                (sha256
                 (base32
                  "128syf9v39gk0z3ip000qpsjbg6l1siyq6c8b0hz41dzg5achyb3"))))
      (build-system r-build-system)
      (propagated-inputs
       `(("r-formula" ,r-formula)
         ("r-ggplot2" ,r-ggplot2)
         ("r-pkgmaker" ,r-pkgmaker)
         ("r-plyr" ,r-plyr)
         ("r-rngtools" ,r-rngtools)
         ("r-scales" ,r-scales)))
      (home-page "https://github.com/shenorrLab/csSAM/")
      (synopsis "Cell type-specific statistical analysis of microarray")
      (description "This package implements the method csSAM that computes
cell-specific differential expression from measured cell proportions using
SAM.")
      ;; Any version
      (license license:lgpl2.1+))))

(define-public r-bseqsc
  (let ((revision "1")
        (commit "fef3f3e38dcf3df37103348b5780937982b43b98"))
    (package
      (name "r-bseqsc")
      (version (git-version "1.0" revision commit))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "https://github.com/shenorrLab/bseqsc")
                      (commit commit)))
                (file-name (git-file-name name version))
                (sha256
                 (base32
                  "1prw13wa20f7wlc3gkkls66n1kxz8d28qrb8icfqdwdnnv8w5qg8"))))
      (build-system r-build-system)
      (propagated-inputs
       `(("r-abind" ,r-abind)
         ("r-annotationdbi" ,r-annotationdbi)
         ("r-biobase" ,r-biobase)
         ("r-cssam" ,r-cssam)
         ("r-dplyr" ,r-dplyr)
         ("r-e1071" ,r-e1071)
         ("r-edger" ,r-edger)
         ("r-ggplot2" ,r-ggplot2)
         ("r-nmf" ,r-nmf)
         ("r-openxlsx" ,r-openxlsx)
         ("r-pkgmaker" ,r-pkgmaker)
         ("r-plyr" ,r-plyr)
         ("r-preprocesscore" ,r-preprocesscore)
         ("r-rngtools" ,r-rngtools)
         ("r-scales" ,r-scales)
         ("r-stringr" ,r-stringr)
         ("r-xbioc" ,r-xbioc)))
      (home-page "https://github.com/shenorrLab/bseqsc")
      (synopsis "Deconvolution of bulk sequencing experiments using single cell data")
      (description "BSeq-sc is a bioinformatics analysis pipeline that
leverages single-cell sequencing data to estimate cell type proportion and
cell type-specific gene expression differences from RNA-seq data from bulk
tissue samples.  This is a companion package to the publication \"A
single-cell transcriptomic map of the human and mouse pancreas reveals inter-
and intra-cell population structure.\" Baron et al. Cell Systems (2016)
@url{https://www.ncbi.nlm.nih.gov/pubmed/27667365}.")
      (license license:gpl2+))))

(define-public porechop
  ;; The recommended way to install is to clone the git repository
  ;; https://github.com/rrwick/Porechop#installation
  (let ((commit "289d5dca4a5fc327f97b3f8cecb68ecaf1014861")
        (revision "1"))
    (package
      (name "porechop")
      (version (git-version "0.2.3" revision commit))
      (source
       (origin
         (method git-fetch)
         (uri (git-reference
               (url "https://github.com/rrwick/Porechop")
               (commit commit)))
         (file-name (git-file-name name version))
         (sha256
          (base32 "05ps43gig0d3ia9x5lj84lb00hbsl6ba9n7y7jz927npxbr2ym23"))))
      (build-system python-build-system)
      (home-page "https://github.com/rrwick/porechop")
      (synopsis "Finding, trimming or splitting adapters, in Oxford Nanopore reads")
      (description
       "The porechop package is a tool for finding and removing adapters from Oxford
Nanopore reads.  Adapters on the ends of reads are trimmed off, and when a read
has an adapter in its middle, it is treated as chimeric and chopped into
separate reads.  Porechop performs thorough alignments to effectively find
adapters, even at low sequence identity.  Porechop also supports demultiplexing
of Nanopore reads that were barcoded with the Native Barcoding Kit, PCR
Barcoding Kit or Rapid Barcoding Kit.")
      (license license:gpl3+))))

(define-public poretools
  ;; The latest release was in 2016 and the latest commit is from 2017
  ;; the recommended way to install is to clone the git repository
  ;; https://poretools.readthedocs.io/en/latest/content/installation.html
  (let ((commit "e426b1f09e86ac259a00c261c79df91510777407")
        (revision "1"))
    (package
      (name "poretools")
      (version (git-version "0.6.0" revision commit))
      (source
       (origin
         (method git-fetch)
         (uri (git-reference
               (url "https://github.com/arq5x/poretools")
               (commit commit)))
         (file-name (git-file-name name version))
         (sha256
          (base32 "0bglj833wxpp3cq430p1d3xp085ls221js2y90w7ir2x5ay8l7am"))))
      (build-system python-build-system)
      ;; requires python >=2.7, <3.0, and the same for python dependencies
      (arguments `(#:python ,python-2))
      (inputs
       `(("hdf5" ,hdf5)))
      (propagated-inputs
       `(("python-dateutil" ,python2-dateutil)
         ("python-h5py" ,python2-h5py)
         ("python-matplotlib" ,python2-matplotlib)
         ("python-pandas" ,python2-pandas)
         ("python-seaborn" ,python2-seaborn)))
      (home-page "https://poretools.readthedocs.io")
      (synopsis "Toolkit for working with nanopore sequencing data")
      (description
       "The MinION from Oxford Nanopore Technologies is a nanopore sequencer.
This @code{poretools} package is a flexible toolkit for exploring datasets
generated by nanopore sequencing devices for the purposes of quality control and
downstream analysis.  Poretools operates directly on the native FAST5, a variant
of the Hierarchical Data Format (HDF5) standard.")
      (license license:expat))))

(define-public jamm
  (package
    (name "jamm")
    (version "1.0.7.6")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/mahmoudibrahim/JAMM")
             (commit (string-append "JAMMv" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "0bsa5mf9n9q5jz7mmacrra41l7r8rac5vgsn6wv1fb52ya58b970"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; there are none
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (delete 'build)
         (replace 'install
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (libexec (string-append out "/libexec/jamm"))
                    (bin (string-append out "/bin")))
               (substitute* '("JAMM.sh"
                              "SignalGenerator.sh")
                 (("^sPath=.*")
                  (string-append "sPath=\"" libexec "\"\n")))
               (for-each (lambda (file)
                           (install-file file libexec))
                         (list "bincalculator.r"
                               "peakfinder.r"
                               "peakhelper.r"
                               "signalmaker.r"
                               "xcorr.r"
                               "xcorrhelper.r"
                               ;; Perl scripts
                               "peakfilter.pl"
                               "readshifter.pl"))

               (for-each
                (lambda (script)
                  (chmod script #o555)
                  (install-file script bin)
                  (wrap-program (string-append bin "/" script)
                    `("PATH" ":" prefix
                      (,(string-append (assoc-ref inputs "coreutils") "/bin")
                       ,(string-append (assoc-ref inputs "gawk") "/bin")
                       ,(string-append (assoc-ref inputs "perl") "/bin")
                       ,(string-append (assoc-ref inputs "r-minimal") "/bin")))
                    `("PERL5LIB" ":" prefix (,(getenv "PERL5LIB")))
                    `("R_LIBS_SITE" ":" prefix (,(getenv "R_LIBS_SITE")))))
                (list "JAMM.sh" "SignalGenerator.sh")))
             #t)))))
    (inputs
     `(("bash" ,bash)
       ("coreutils" ,coreutils)
       ("gawk" ,gawk)
       ("perl" ,perl)
       ("r-minimal" ,r-minimal)
       ;;("r-parallel" ,r-parallel)
       ("r-signal" ,r-signal)
       ("r-mclust" ,r-mclust)))
    (home-page "https://github.com/mahmoudibrahim/JAMM")
    (synopsis "Peak finder for NGS datasets")
    (description
     "JAMM is a peak finder for next generation sequencing datasets (ChIP-Seq,
ATAC-Seq, DNase-Seq, etc.) that can integrate replicates and assign peak
boundaries accurately.  JAMM is applicable to both broad and narrow
datasets.")
    (license license:gpl3+)))

(define-public ngless
  (package
    (name "ngless")
    (version "1.1.0")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://gitlab.com/ngless/ngless.git")
             (commit (string-append "v" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "1wim8wpqyff080dfcazynrmjwqas38m24m0v350w245mmhrapdma"))))
    (build-system haskell-build-system)
    (arguments
     `(#:haddock? #f ; The haddock phase fails with: NGLess/CmdArgs.hs:20:1:
                     ; error: parse error on input import
                     ; import Options.Applicative
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'create-Versions.hs
           (lambda _
             (substitute* "Makefile"
               (("BWA_VERSION = .*")
                (string-append "BWA_VERSION = "
                               ,(package-version bwa) "\n"))
               (("SAM_VERSION = .*")
                (string-append "SAM_VERSION = "
                               ,(package-version samtools) "\n"))
               (("PRODIGAL_VERSION = .*")
                (string-append "PRODIGAL_VERSION = "
                               ,(package-version prodigal) "\n"))
               (("MINIMAP2_VERSION = .*")
                (string-append "MINIMAP2_VERSION = "
                               ,(package-version minimap2) "\n")))
             (invoke "make" "NGLess/Dependencies/Versions.hs")
             #t))
         (add-after 'create-Versions.hs 'create-cabal-file
           (lambda _ (invoke "hpack") #t))
         ;; These tools are expected to be installed alongside ngless.
         (add-after 'install 'link-tools
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let ((bin (string-append (assoc-ref outputs "out") "/bin/")))
               (symlink (string-append (assoc-ref inputs "prodigal")
                                       "/bin/prodigal")
                        (string-append bin "ngless-" ,version "-prodigal"))
               (symlink (string-append (assoc-ref inputs "minimap2")
                                       "/bin/minimap2")
                        (string-append bin "ngless-" ,version "-minimap2"))
               (symlink (string-append (assoc-ref inputs "samtools")
                                       "/bin/samtools")
                        (string-append bin "ngless-" ,version "-samtools"))
               (symlink (string-append (assoc-ref inputs "bwa")
                                       "/bin/bwa")
                        (string-append bin "ngless-" ,version "-bwa"))
               #t))))))
    (inputs
     `(("prodigal" ,prodigal)
       ("bwa" ,bwa)
       ("samtools" ,samtools)
       ("minimap2" ,minimap2)
       ("ghc-aeson" ,ghc-aeson)
       ("ghc-ansi-terminal" ,ghc-ansi-terminal)
       ("ghc-async" ,ghc-async)
       ("ghc-atomic-write" ,ghc-atomic-write)
       ("ghc-bytestring-lexing" ,ghc-bytestring-lexing)
       ("ghc-conduit" ,ghc-conduit)
       ("ghc-conduit-algorithms" ,ghc-conduit-algorithms)
       ("ghc-conduit-extra" ,ghc-conduit-extra)
       ("ghc-configurator" ,ghc-configurator)
       ("ghc-convertible" ,ghc-convertible)
       ("ghc-data-default" ,ghc-data-default)
       ("ghc-diagrams-core" ,ghc-diagrams-core)
       ("ghc-diagrams-lib" ,ghc-diagrams-lib)
       ("ghc-diagrams-svg" ,ghc-diagrams-svg)
       ("ghc-double-conversion" ,ghc-double-conversion)
       ("ghc-edit-distance" ,ghc-edit-distance)
       ("ghc-either" ,ghc-either)
       ("ghc-errors" ,ghc-errors)
       ("ghc-extra" ,ghc-extra)
       ("ghc-filemanip" ,ghc-filemanip)
       ("ghc-file-embed" ,ghc-file-embed)
       ("ghc-gitrev" ,ghc-gitrev)
       ("ghc-hashtables" ,ghc-hashtables)
       ("ghc-http-conduit" ,ghc-http-conduit)
       ("ghc-inline-c" ,ghc-inline-c)
       ("ghc-inline-c-cpp" ,ghc-inline-c-cpp)
       ("ghc-intervalmap" ,ghc-intervalmap)
       ("ghc-missingh" ,ghc-missingh)
       ("ghc-optparse-applicative" ,ghc-optparse-applicative)
       ("ghc-regex" ,ghc-regex)
       ("ghc-safe" ,ghc-safe)
       ("ghc-safeio" ,ghc-safeio)
       ("ghc-strict" ,ghc-strict)
       ("ghc-tar" ,ghc-tar)
       ("ghc-tar-conduit" ,ghc-tar-conduit)
       ("ghc-unliftio" ,ghc-unliftio)
       ("ghc-unliftio-core" ,ghc-unliftio-core)
       ("ghc-vector" ,ghc-vector)
       ("ghc-yaml" ,ghc-yaml)
       ("ghc-zlib" ,ghc-zlib)))
    (propagated-inputs
     `(("r-r6" ,r-r6)
       ("r-hdf5r" ,r-hdf5r)
       ("r-iterators" ,r-iterators)
       ("r-itertools" ,r-itertools)
       ("r-matrix" ,r-matrix)))
    (native-inputs
     `(("ghc-hpack" ,ghc-hpack)
       ("ghc-quickcheck" ,ghc-quickcheck)
       ("ghc-test-framework" ,ghc-test-framework)
       ("ghc-test-framework-hunit",ghc-test-framework-hunit)
       ("ghc-test-framework-quickcheck2" ,ghc-test-framework-quickcheck2)
       ("ghc-test-framework-th" ,ghc-test-framework-th)))
    (home-page "https://gitlab.com/ngless/ngless")
    (synopsis "DSL for processing next-generation sequencing data")
    (description "Ngless is a domain-specific language for
@dfn{next-generation sequencing} (NGS) data processing.")
    (license license:expat)))

(define-public filtlong
  ;; The recommended way to install is to clone the git repository
  ;; https://github.com/rrwick/Filtlong#installation
  ;; and the lastest release is more than nine months old
  (let ((commit "d1bb46dfe8bc7efe6257b5ce222c04bfe8aedaab")
        (revision "1"))
    (package
      (name "filtlong")
      (version (git-version "0.2.0" revision commit))
      (source
       (origin
         (method git-fetch)
         (uri (git-reference
               (url "https://github.com/rrwick/Filtlong")
               (commit commit)))
         (file-name (git-file-name name version))
         (sha256
          (base32 "1xr92r820x8qlkcr3b57iw223yq8vjgyi42jr79w2xgw47qzr575"))))
      (build-system gnu-build-system)
      (arguments
       `(#:tests? #f                    ; no check target
         #:phases
         (modify-phases %standard-phases
           (delete 'configure)
           (replace 'install
             (lambda* (#:key outputs #:allow-other-keys)
               (let* ((out (assoc-ref outputs "out"))
                      (bin (string-append out "/bin"))
                      (scripts (string-append out "/share/filtlong/scripts")))
                 (install-file "bin/filtlong" bin)
                 (install-file "scripts/histogram.py" scripts)
                 (install-file "scripts/read_info_histograms.sh" scripts))
               #t))
           (add-after 'install 'wrap-program
             (lambda* (#:key inputs outputs #:allow-other-keys)
               (let* ((out (assoc-ref outputs "out"))
                      (path (getenv "PYTHONPATH")))
                 (wrap-program (string-append out
                                              "/share/filtlong/scripts/histogram.py")
                   `("PYTHONPATH" ":" prefix (,path))))
               #t))
           (add-before 'check 'patch-tests
             (lambda _
               (substitute* "scripts/read_info_histograms.sh"
                 (("awk") (which "gawk")))
               #t)))))
      (inputs
       `(("gawk" ,gawk)                 ;for read_info_histograms.sh
         ("python" ,python-2)           ;required for histogram.py
         ("zlib" ,zlib)))
      (home-page "https://github.com/rrwick/Filtlong/")
      (synopsis "Tool for quality filtering of Nanopore and PacBio data")
      (description
       "The Filtlong package is a tool for filtering long reads by quality.
It can take a set of long reads and produce a smaller, better subset.  It uses
both read length (longer is better) and read identity (higher is better) when
choosing which reads pass the filter.")
      (license (list license:gpl3       ;filtlong
                     license:asl2.0))))) ;histogram.py

(define-public nanopolish
  ;; The recommended way to install is to clone the git repository
  ;; <https://github.com/jts/nanopolish#installing-a-particular-release>.
  ;; Also, the differences between release and current version seem to be
  ;; significant.
  (let ((commit "6331dc4f15b9dfabb954ba3fae9d76b6c3ca6377")
        (revision "1"))
    (package
      (name "nanopolish")
      (version (git-version "0.11.1" revision commit))
      (source
       (origin
         (method git-fetch)
         (uri (git-reference
               (url "https://github.com/jts/nanopolish")
               (commit commit)
               (recursive? #t)))
         (file-name (git-file-name name version))
         (sha256
          (base32 "15ikl3d37y49pwd7vx36xksgsqajhf24q7qqsnpl15dqqyy5qgbc"))
         (modules '((guix build utils)))
         (snippet
          '(begin
             (delete-file-recursively "htslib")
             #t))))
      (build-system gnu-build-system)
      (arguments
       `(#:make-flags
         `("HDF5=noinstall" "EIGEN=noinstall" "HTS=noinstall" "CC=gcc")
         #:tests? #f                    ; no check target
         #:phases
         (modify-phases %standard-phases
           (add-after 'unpack 'find-eigen
             (lambda* (#:key inputs #:allow-other-keys)
               (setenv "CPATH"
                       (string-append (assoc-ref inputs "eigen")
                                      "/include/eigen3:"
                                      (or (getenv "CPATH") "")))
               #t))
           (delete 'configure)
           (replace 'install
             (lambda* (#:key outputs #:allow-other-keys)
               (let* ((out (assoc-ref outputs "out"))
                      (bin (string-append out "/bin"))
                      (scripts (string-append out "/share/nanopolish/scripts")))

                 (install-file "nanopolish" bin)
                 (for-each (lambda (file) (install-file file scripts))
                           (find-files "scripts" ".*"))
                 #t)))
           (add-after 'install 'wrap-programs
             (lambda* (#:key outputs #:allow-other-keys)
               (for-each (lambda (file)
                           (wrap-program file `("PYTHONPATH" ":" prefix (,path))))
                         (find-files "/share/nanopolish/scripts" "\\.py"))
               (for-each (lambda (file)
                           (wrap-program file `("PERL5LIB" ":" prefix (,path))))
                         (find-files  "/share/nanopolish/scripts" "\\.pl"))
               #t)))))
      (inputs
       `(("eigen" ,eigen)
         ("hdf5" ,hdf5)
         ("htslib" ,htslib)
         ("perl" ,perl)
         ("python" ,python-wrapper)
         ("python-biopython" ,python-biopython)
         ("python-numpy" ,python-numpy)
         ("python-pysam" ,python-pysam)
         ("python-scikit-learn" , python-scikit-learn)
         ("python-scipy" ,python-scipy)
         ("zlib" ,zlib)))
      (home-page "https://github.com/jts/nanopolish")
      (synopsis "Signal-level analysis of Oxford Nanopore sequencing data")
      (description
       "This package analyses the Oxford Nanopore sequencing data at signal-level.
Nanopolish can calculate an improved consensus sequence for a draft genome
assembly, detect base modifications, call SNPs (Single nucleotide
polymorphisms) and indels with respect to a reference genome and more.")
      (license license:expat))))

(define-public cnvkit
  (package
    (name "cnvkit")
    (version "0.9.5")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/etal/cnvkit")
             (commit (string-append "v" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32 "0g2f78k68yglmj4fsfmgs8idqv3di9aj53fg0ld0hqljg8chhh82"))))
    (build-system python-build-system)
    (propagated-inputs
     `(("python-biopython" ,python-biopython)
       ("python-future" ,python-future)
       ("python-matplotlib" ,python-matplotlib)
       ("python-numpy" ,python-numpy)
       ("python-reportlab" ,python-reportlab)
       ("python-pandas" ,python-pandas)
       ("python-pysam" ,python-pysam)
       ("python-pyfaidx" ,python-pyfaidx)
       ("python-scipy" ,python-scipy)
       ;; R packages
       ("r-dnacopy" ,r-dnacopy)))
    (home-page "https://cnvkit.readthedocs.org/")
    (synopsis "Copy number variant detection from targeted DNA sequencing")
    (description
     "CNVkit is a Python library and command-line software toolkit to infer
and visualize copy number from high-throughput DNA sequencing data.  It is
designed for use with hybrid capture, including both whole-exome and custom
target panels, and short-read sequencing platforms such as Illumina and Ion
Torrent.")
    (license license:asl2.0)))

(define-public python-pyfit-sne
  (package
    (name "python-pyfit-sne")
    (version "1.0.1")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/KlugerLab/pyFIt-SNE")
             (commit version)))
       (file-name (git-file-name name version))
       (sha256
        (base32 "13wh3qkzs56azmmgnxib6xfr29g7xh09sxylzjpni5j0pp0rc5qw"))))
    (build-system python-build-system)
    (propagated-inputs
     `(("python-numpy" ,python-numpy)))
    (inputs
     `(("fftw" ,fftw)))
    (native-inputs
     `(("python-cython" ,python-cython)))
    (home-page "https://github.com/KlugerLab/pyFIt-SNE")
    (synopsis "FFT-accelerated Interpolation-based t-SNE")
    (description
     "t-Stochastic Neighborhood Embedding (t-SNE) is a highly successful
method for dimensionality reduction and visualization of high dimensional
datasets.  A popular implementation of t-SNE uses the Barnes-Hut algorithm to
approximate the gradient at each iteration of gradient descent.  This package
is a Cython wrapper for FIt-SNE.")
    (license license:bsd-4)))

(define-public bbmap
  (package
    (name "bbmap")
    (version "35.82")
    (source (origin
              (method url-fetch)
              (uri (string-append
                    "mirror://sourceforge/bbmap/BBMap_" version ".tar.gz"))
              (sha256
               (base32
                "1q4rfhxcb6z3gm8zg2davjz98w22lkf4hm9ikxz9kdl93pil3wkd"))))
    (build-system ant-build-system)
    (arguments
     `(#:build-target "dist"
       #:tests? #f ; there are none
       #:make-flags
       (list (string-append "-Dmpijar="
                            (assoc-ref %build-inputs "java-openmpi")
                            "/lib/mpi.jar"))
       #:modules ((guix build ant-build-system)
                  (guix build utils)
                  (guix build java-utils))
       #:phases
       (modify-phases %standard-phases
         (add-after 'build 'build-jni-library
           (lambda _
             (with-directory-excursion "jni"
               (invoke "make" "-f" "makefile.linux"))))
         ;; There is no install target
         (replace 'install (install-jars "dist"))
         (add-after 'install 'install-scripts-and-documentation
           (lambda* (#:key outputs #:allow-other-keys)
             (substitute* "calcmem.sh"
               (("\\| awk ") (string-append "| " (which "awk") " ")))
             (let* ((scripts (find-files "." "\\.sh$"))
                    (out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin"))
                    (doc (string-append out "/share/doc/bbmap"))
                    (jni (string-append out "/lib/jni")))
               (substitute* scripts
                 (("\\$DIR\"\"docs") doc)
                 (("^CP=.*")
                  (string-append "CP=" out "/share/java/BBTools.jar\n"))
                 (("^NATIVELIBDIR.*")
                  (string-append "NATIVELIBDIR=" jni "\n"))
                 (("CMD=\"java")
                  (string-append "CMD=\"" (which "java"))))
               (for-each (lambda (script) (install-file script bin)) scripts)

               ;; Install JNI library
               (install-file "jni/libbbtoolsjni.so" jni)

               ;; Install documentation
               (install-file "docs/readme.txt" doc)
               (copy-recursively "docs/guides" doc))
             #t)))
       #:jdk ,openjdk11))
    (inputs
     `(("gawk" ,gawk)
       ("java-eclipse-jdt-core" ,java-eclipse-jdt-core)
       ("java-eclipse-jdt-compiler-apt" ,java-eclipse-jdt-compiler-apt)
       ("java-openmpi" ,java-openmpi)))
    (home-page "https://sourceforge.net/projects/bbmap/")
    (synopsis "Aligner and other tools for short sequencing reads")
    (description
     "This package provides bioinformatic tools to align, deduplicate,
reformat, filter and normalize DNA and RNA-seq data.  It includes the
following tools: BBMap, a short read aligner for DNA and RNA-seq data; BBNorm,
a kmer-based error-correction and normalization tool; Dedupe, a tool to
simplify assemblies by removing duplicate or contained subsequences that share
a target percent identity; Reformat, to convert reads between
fasta/fastq/scarf/fasta+qual/sam, interleaved/paired, and ASCII-33/64, at over
500 MB/s; and BBDuk, a tool to filter, trim, or mask reads with kmer matches
to an artifact/contaminant file.")
    (license license:bsd-3)))

(define-public velvet
  (package
    (name "velvet")
    (version "1.2.10")
    (source (origin
              (method url-fetch)
              (uri (string-append "https://www.ebi.ac.uk/~zerbino/velvet/"
                                  "velvet_" version ".tgz"))
              (sha256
               (base32
                "0h3njwy66p6bx14r3ar1byb0ccaxmxka4c65rn4iybyiqa4d8kc8"))
              ;; Delete bundled libraries
              (modules '((guix build utils)))
              (snippet
               '(begin
                  (delete-file "Manual.pdf")
                  (delete-file-recursively "third-party")
                  #t))))
    (build-system gnu-build-system)
    (arguments
     `(#:make-flags '("OPENMP=t")
       #:test-target "test"
       #:phases
       (modify-phases %standard-phases
         (delete 'configure)
         (add-after 'unpack 'fix-zlib-include
           (lambda _
             (substitute* "src/binarySequences.c"
               (("../third-party/zlib-1.2.3/zlib.h") "zlib.h"))
             #t))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin"))
                    (doc (string-append out "/share/doc/velvet")))
               (mkdir-p bin)
               (mkdir-p doc)
               (install-file "velveth" bin)
               (install-file "velvetg" bin)
               (install-file "Manual.pdf" doc)
               (install-file "Columbus_manual.pdf" doc)
               #t))))))
    (inputs
     `(("openmpi" ,openmpi)
       ("zlib" ,zlib)))
    (native-inputs
     `(("texlive" ,(texlive-union (list texlive-latex-graphics
                                        texlive-latex-hyperref)))))
    (home-page "https://www.ebi.ac.uk/~zerbino/velvet/")
    (synopsis "Nucleic acid sequence assembler for very short reads")
    (description
     "Velvet is a de novo genomic assembler specially designed for short read
sequencing technologies, such as Solexa or 454.  Velvet currently takes in
short read sequences, removes errors then produces high quality unique
contigs.  It then uses paired read information, if available, to retrieve the
repeated areas between contigs.")
    (license license:gpl2+)))

(define-public python-velocyto
  (package
    (name "python-velocyto")
    (version "0.17.17")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "velocyto" version))
       (sha256
        (base32
         "0fgygyzqgrq32dv6a00biq1p1cwi6kbl5iqblxq1kklj6b2mzmhs"))))
    (build-system python-build-system)
    (native-inputs
     `(("python-joblib" ,python-joblib)))
    (propagated-inputs
     `(("python-click" ,python-click)
       ("python-cython" ,python-cython)
       ("python-h5py" ,python-h5py)
       ("python-loompy" ,python-loompy)
       ("python-matplotlib" ,python-matplotlib)
       ("python-numba" ,python-numba)
       ("python-numpy" ,python-numpy)
       ("python-pandas" ,python-pandas)
       ("python-pysam" ,python-pysam)
       ("python-scikit-learn" ,python-scikit-learn)
       ("python-scipy" ,python-scipy)))
    (home-page "https://github.com/velocyto-team/velocyto.py")
    (synopsis "RNA velocity analysis for single cell RNA-seq data")
    (description
     "Velocyto is a library for the analysis of RNA velocity.  Velocyto
includes a command line tool and an analysis pipeline.")
    (license license:bsd-2)))

(define-public arriba
  (package
    (name "arriba")
    (version "1.0.1")
    (source
     (origin
       (method url-fetch)
       (uri (string-append "https://github.com/suhrig/arriba/releases/"
                           "download/v" version "/arriba_v" version ".tar.gz"))
       (sha256
        (base32
         "0jx9656ry766vb8z08m1c3im87b0c82qpnjby9wz4kcz8vn87dx2"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; there are none
       #:phases
       (modify-phases %standard-phases
         (replace 'configure
           (lambda* (#:key inputs #:allow-other-keys)
             (let ((htslib (assoc-ref inputs "htslib")))
               (substitute* "Makefile"
                 (("-I\\$\\(HTSLIB\\)/htslib")
                  (string-append "-I" htslib "/include/htslib"))
                 ((" \\$\\(HTSLIB\\)/libhts.a")
                  (string-append " " htslib "/lib/libhts.so"))))
             (substitute* "run_arriba.sh"
               (("^STAR ") (string-append (which "STAR") " "))
               (("samtools --version-only")
                (string-append (which "samtools") " --version-only"))
               (("samtools index")
                (string-append (which "samtools") " index"))
               (("samtools sort")
                (string-append (which "samtools") " sort")))
             #t))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((bin (string-append (assoc-ref outputs "out") "/bin")))
               (install-file "arriba" bin)
               (install-file "run_arriba.sh" bin)
               (install-file "draw_fusions.R" bin)
               (wrap-program (string-append bin "/draw_fusions.R")
                 `("R_LIBS_SITE" ":" prefix (,(getenv "R_LIBS_SITE")))))
             #t)))))
    (inputs
     `(("htslib" ,htslib)
       ("r-minimal" ,r-minimal)
       ("r-circlize" ,r-circlize)
       ("r-genomicalignments" ,r-genomicalignments)
       ("r-genomicranges" ,r-genomicranges)
       ("samtools" ,samtools)
       ("star" ,star)
       ("zlib" ,zlib)))
    (home-page "https://github.com/suhrig/arriba")
    (synopsis "Gene fusion detection from RNA-Seq data ")
    (description
     "Arriba is a command-line tool for the detection of gene fusions from
RNA-Seq data.  It was developed for the use in a clinical research setting.
Therefore, short runtimes and high sensitivity were important design criteria.
It is based on the fast STAR aligner and the post-alignment runtime is
typically just around two minutes.  In contrast to many other fusion detection
tools which build on STAR, Arriba does not require to reduce the
@code{alignIntronMax} parameter of STAR to detect small deletions.")
    ;; All code is under the Expat license with the exception of
    ;; "draw_fusions.R", which is under GPLv3.
    (license (list license:expat license:gpl3))))

(define-public adapterremoval
  (package
    (name "adapterremoval")
    (version "2.3.0")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/MikkelSchubert/adapterremoval")
             (commit (string-append "v" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "1nf3ki5pfzalhrx2fr1y6pfqfi133yj2m7q4fj9irf5fb94bapwr"))))
    (build-system gnu-build-system)
    (arguments
     `(#:make-flags (list "COLOR_BUILD=no"
                          (string-append "PREFIX="
                                         (assoc-ref %outputs "out")))
       #:test-target "test"
       #:phases
       (modify-phases %standard-phases
         (delete 'configure))))
    (inputs
     `(("zlib" ,zlib)))
    (home-page "https://adapterremoval.readthedocs.io/")
    (synopsis "Rapid sequence adapter trimming, identification, and read merging")
    (description
     "This program searches for and removes remnant adapter sequences from
@dfn{High-Throughput Sequencing} (HTS) data and (optionally) trims low quality
bases from the 3' end of reads following adapter removal.  AdapterRemoval can
analyze both single end and paired end data, and can be used to merge
overlapping paired-ended reads into (longer) consensus sequences.
Additionally, the AdapterRemoval may be used to recover a consensus adapter
sequence for paired-ended data, for which this information is not available.")
    (license license:gpl3+)))

(define-public pplacer
  (let ((commit "807f6f3"))
    (package
      (name "pplacer")
      ;; The commit should be updated with each version change.
      (version "1.1.alpha19")
      (source
       (origin
         (method git-fetch)
         (uri (git-reference
               (url "https://github.com/matsen/pplacer")
               (commit (string-append "v" version))))
         (file-name (git-file-name name version))
         (sha256
          (base32 "11ppbbbx20p2g9wj3ff64dhnarb12q79v7qh4rk0gj6lkbz4n7cn"))))
      (build-system ocaml-build-system)
      (arguments
       `(#:modules ((guix build ocaml-build-system)
                    (guix build utils)
                    (ice-9 ftw))
         #:phases
         (modify-phases %standard-phases
           (delete 'configure)
           (add-after 'unpack 'fix-build-with-latest-ocaml
             (lambda _
               (substitute* "myocamlbuild.ml"
                 (("dep \\[\"c_pam\"\\]" m)
                  (string-append "flag [\"ocaml\"; \"compile\"] (A \"-unsafe-string\");\n"
                                 m))
                 (("let run_and_read" m)
                  (string-append "
let split s ch =
  let x = ref [] in
  let rec go s =
    let pos = String.index s ch in
    x := (String.before s pos)::!x;
    go (String.after s (pos + 1))
  in
  try go s
  with Not_found -> !x
let split_nl s = split s '\\n'
let before_space s =
  try String.before s (String.index s ' ')
  with Not_found -> s

" m))
                 (("run_and_read \"ocamlfind list \\| cut -d' ' -f1\"" m)
                  (string-append "List.map before_space (split_nl & " m ")"))
                 (("    blank_sep_strings &") "")
                 (("      Lexing.from_string &") ""))
               #t))
           (add-after 'unpack 'replace-bundled-cddlib
             (lambda* (#:key inputs #:allow-other-keys)
               (let* ((cddlib-src (assoc-ref inputs "cddlib-src"))
                      (local-dir "cddlib_guix"))
                 (mkdir local-dir)
                 (with-directory-excursion local-dir
                   (invoke "tar" "xvf" cddlib-src))
                 (let ((cddlib-src-folder
                        (string-append local-dir "/"
                                       (list-ref (scandir local-dir) 2)
                                       "/lib-src")))
                   (for-each make-file-writable (find-files "cdd_src" ".*"))
                   (for-each
                    (lambda (file)
                      (copy-file file
                                 (string-append "cdd_src/" (basename file))))
                    (find-files cddlib-src-folder ".*[ch]$")))
                 #t)))
           (add-after 'unpack 'fix-makefile
             (lambda _
               ;; Remove system calls to 'git'.
               (substitute* "Makefile"
                 (("^DESCRIPT:=pplacer-.*")
                  (string-append
                   "DESCRIPT:=pplacer-$(shell uname)-v" ,version "\n")))
               (substitute* "myocamlbuild.ml"
                 (("git describe --tags --long .*\\\" with")
                  (string-append
                   "echo -n v" ,version "-" ,commit "\" with")))
               #t))
           (replace 'install
             (lambda* (#:key outputs #:allow-other-keys)
               (let* ((out (assoc-ref outputs "out"))
                      (bin (string-append out "/bin")))
                 (copy-recursively "bin" bin))
               #t)))
       #:ocaml ,ocaml-4.07
       #:findlib ,ocaml4.07-findlib))
      (inputs
       `(("zlib" ,zlib "static")
         ("gsl" ,gsl)
         ("ocaml-ounit" ,(package-with-ocaml4.07 ocaml-ounit))
         ("ocaml-batteries" ,(package-with-ocaml4.07 ocaml-batteries))
         ("ocaml-camlzip" ,(package-with-ocaml4.07 camlzip))
         ("ocaml-csv" ,(package-with-ocaml4.07 ocaml-csv))
         ("ocaml-sqlite3" ,ocaml4.07-sqlite3)
         ("ocaml-xmlm" ,(package-with-ocaml4.07 ocaml-xmlm))
         ("ocaml-mcl" ,(package-with-ocaml4.07 ocaml-mcl))
         ("ocaml-gsl" ,ocaml4.07-gsl-1)))
      (native-inputs
       `(("cddlib-src" ,(package-source cddlib))
         ("ocamlbuild" ,(package-with-ocaml4.07 ocamlbuild))
         ("pkg-config" ,pkg-config)))
      (propagated-inputs
       `(("pplacer-scripts" ,pplacer-scripts)))
      (synopsis "Phylogenetic placement of biological sequences")
      (description
       "Pplacer places query sequences on a fixed reference phylogenetic tree
to maximize phylogenetic likelihood or posterior probability according to a
reference alignment.  Pplacer is designed to be fast, to give useful
information about uncertainty, and to offer advanced visualization and
downstream analysis.")
      (home-page "https://matsen.fhcrc.org/pplacer/")
      (license license:gpl3))))

;; This package is installed alongside 'pplacer'.  It is a separate package so
;; that it can use the python-build-system for the scripts that are
;; distributed alongside the main OCaml binaries.
(define pplacer-scripts
  (package
    (inherit pplacer)
    (name "pplacer-scripts")
    (build-system python-build-system)
    (arguments
     `(#:python ,python-2
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'enter-scripts-dir
           (lambda _ (chdir "scripts") #t))
         (replace 'check
           (lambda _ (invoke "python" "-m" "unittest" "discover" "-v") #t))
         (add-after 'install 'wrap-executables
           (lambda* (#:key inputs outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin")))
               (let ((path (string-append
                            (assoc-ref inputs "hmmer") "/bin:"
                            (assoc-ref inputs "infernal") "/bin")))
                 (display path)
                 (wrap-program (string-append bin "/refpkg_align.py")
                   `("PATH" ":" prefix (,path))))
               (let ((path (string-append
                            (assoc-ref inputs "hmmer") "/bin")))
                 (wrap-program (string-append bin "/hrefpkg_query.py")
                   `("PATH" ":" prefix (,path)))))
             #t)))))
    (inputs
     `(("infernal" ,infernal)
       ("hmmer" ,hmmer)))
    (propagated-inputs
     `(("python-biopython" ,python2-biopython)
       ("taxtastic" ,taxtastic)))
    (synopsis "Pplacer Python scripts")))

(define-public python2-checkm-genome
  (package
    (name "python2-checkm-genome")
    (version "1.0.13")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "checkm-genome" version))
       (sha256
        (base32
         "0bm8gpxjmzxsxxl8lzwqhgx8g1dlnmp6znz7wv3hgb0gdjbf9dzz"))))
    (build-system python-build-system)
    (arguments
     `(#:python ,python-2
       #:tests? #f))                    ; some tests are interactive
    (propagated-inputs
     `(("python-dendropy" ,python2-dendropy)
       ("python-matplotlib" ,python2-matplotlib)
       ("python-numpy" ,python2-numpy)
       ("python-pysam" ,python2-pysam)
       ("python-scipy" ,python2-scipy)))
    (home-page "https://pypi.org/project/Checkm/")
    (synopsis "Assess the quality of putative genome bins")
    (description
     "CheckM provides a set of tools for assessing the quality of genomes
recovered from isolates, single cells, or metagenomes.  It provides robust
estimates of genome completeness and contamination by using collocated sets of
genes that are ubiquitous and single-copy within a phylogenetic lineage.
Assessment of genome quality can also be examined using plots depicting key
genomic characteristics (e.g., GC, coding density) which highlight sequences
outside the expected distributions of a typical genome.  CheckM also provides
tools for identifying genome bins that are likely candidates for merging based
on marker set compatibility, similarity in genomic characteristics, and
proximity within a reference genome.")
    (license license:gpl3+)))

(define-public umi-tools
  (package
    (name "umi-tools")
    (version "1.0.0")
    (source
     (origin
       (method url-fetch)
       (uri (pypi-uri "umi_tools" version))
       (sha256
        (base32
         "08y3vz1vcx09whmbsn722lcs6jl9wyrh9i4p3k8j4cb1i32bij4a"))))
    (build-system python-build-system)
    (inputs
     `(("python-pandas" ,python-pandas)
       ("python-future" ,python-future)
       ("python-scipy" ,python-scipy)
       ("python-matplotlib" ,python-matplotlib)
       ("python-regex" ,python-regex)
       ("python-pysam" ,python-pysam)))
    (native-inputs
     `(("python-cython" ,python-cython)))
    (home-page "https://github.com/CGATOxford/UMI-tools")
    (synopsis "Tools for analyzing unique modular identifiers")
    (description "This package provides tools for dealing with @dfn{Unique
Molecular Identifiers} (UMIs) and @dfn{Random Molecular Tags} (RMTs) in
genetic sequences.  There are six tools: the @code{extract} and
@code{whitelist} commands are used to prepare a fastq containing UMIs @code{+/-}
cell barcodes for alignment.  The remaining commands, @code{group},
@code{dedup}, and @{count}/@code{count_tab}, are used to identify PCR
duplicates using the UMIs and perform different levels of analysis depending
on the needs of the user.")
    (license license:expat)))

(define-public ataqv
  (package
    (name "ataqv")
    (version "1.0.0")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/ParkerLab/ataqv")
             (commit version)))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "031xr6jx1aprh26y5b1lv3gzrlmzg4alfl73vvshymx8cq8asrqi"))))
    (build-system gnu-build-system)
    (arguments
     `(#:make-flags
       (list (string-append "prefix=" (assoc-ref %outputs "out"))
             (string-append "BOOST_ROOT="
                            (assoc-ref %build-inputs "boost"))
             (string-append "HTSLIB_ROOT="
                            (assoc-ref %build-inputs "htslib")))
       #:test-target "test"
       #:phases
       (modify-phases %standard-phases
         (delete 'configure))))
    (inputs
     `(("boost" ,boost)
       ("htslib" ,htslib)
       ("ncurses" ,ncurses)
       ("zlib" ,zlib)))
    (native-inputs
     `(("lcov" ,lcov)))
    (home-page "https://github.com/ParkerLab/ataqv")
    (synopsis "Toolkit for quality control and visualization of ATAC-seq data")
    (description "This package provides a toolkit for measuring and comparing
ATAC-seq results.  It was written to make it easier to spot differences that
might be caused by ATAC-seq library prep or sequencing.  The main program,
@code{ataqv}, examines aligned reads and reports some basic metrics.")
    (license license:gpl3+)))

(define-public r-psiplot
  (package
    (name "r-psiplot")
    (version "2.3.0")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/kcha/psiplot")
             (commit (string-append "v" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32 "08438h16cfry5kqh3y9hs8q1b1a8bxhblsm75knviz5r6q0n1jxh"))))
    (build-system r-build-system)
    (propagated-inputs
     `(("r-mass" ,r-mass)
       ("r-dplyr" ,r-dplyr)
       ("r-tidyr" ,r-tidyr)
       ("r-purrr" ,r-purrr)
       ("r-readr" ,r-readr)
       ("r-magrittr" ,r-magrittr)
       ("r-ggplot2" ,r-ggplot2)))
    (home-page "https://github.com/kcha/psiplot")
    (synopsis "Plot percent spliced-in values of alternatively-spliced exons")
    (description
     "PSIplot is an R package for generating plots of @dfn{percent
spliced-in} (PSI) values of alternatively-spliced exons that were computed by
vast-tools, an RNA-Seq pipeline for alternative splicing analysis.  The plots
are generated using @code{ggplot2}.")
    (license license:expat)))

(define-public python-ont-fast5-api
  (package
    (name "python-ont-fast5-api")
    (version "1.4.4")
    (source
     (origin
       (method git-fetch)
       (uri (git-reference
             (url "https://github.com/nanoporetech/ont_fast5_api")
             (commit (string-append "release_" version))))
       (file-name (git-file-name name version))
       (sha256
        (base32
         "03cbq4zbbwhll8ml2m9k8sa31mirsvcbjkrq1yna0kkzz9fad5fm"))))
    (build-system python-build-system)
    (propagated-inputs
     `(("python-numpy" ,python-numpy)
       ("python-six" ,python-six)
       ("python-h5py" ,python-h5py)
       ("python-progressbar33" ,python-progressbar33)))
    (home-page "https://github.com/nanoporetech/ont_fast5_api")
    (synopsis "Interface to HDF5 files of the Oxford Nanopore fast5 file format")
    (description
     "This package provides a concrete implementation of the fast5 file schema
using the generic @code{h5py} library, plain-named methods to interact with
and reflect the fast5 file schema, and tools to convert between
@code{multi_read} and @code{single_read} formats.")
    (license license:mpl2.0)))

(define-public tbsp
  (let ((commit "ec8fff4410cfb13a677dbbb95cbbc60217e64907")
        (revision "1"))
    (package
      (name "tbsp")
      (version (git-version "1.0.0" revision commit))
      (source
       (origin
         (method git-fetch)
         (uri (git-reference
               (url "https://github.com/phoenixding/tbsp")
               (commit commit)))
         (file-name (git-file-name name version))
         (sha256
          (base32
           "025ym14x8gbd6hb55lsinqj6f5qzw36i10klgs7ldzxxd7s39ki1"))))
      (build-system python-build-system)
      (arguments '(#:tests? #f))        ; no tests included
      (inputs
       `(("python-matplotlib" ,python-matplotlib)
         ("python-networkx" ,python-networkx)
         ("python-numpy" ,python-numpy)
         ("python-pybigwig" ,python-pybigwig)
         ("python-biopython" ,python-biopython)
         ("python-scikit-learn" ,python-scikit-learn)
         ("python-scipy" ,python-scipy)))
      (home-page "https://github.com/phoenixding/tbsp/")
      (synopsis "SNP-based trajectory inference")
      (description
       "Several studies focus on the inference of developmental and response
trajectories from single cell RNA-Seq (scRNA-Seq) data.  A number of
computational methods, often referred to as pseudo-time ordering, have been
developed for this task.  CRISPR has also been used to reconstruct lineage
trees by inserting random mutations.  The tbsp package implements an
alternative method to detect significant, cell type specific sequence
mutations from scRNA-Seq data.")
      (license license:expat))))

(define-public tabixpp
  (package
   (name "tabixpp")
   (version "1.1.0")
   (source (origin
     (method git-fetch)
     (uri (git-reference
           (url "https://github.com/ekg/tabixpp")
           (commit (string-append "v" version))))
     (file-name (git-file-name name version))
     (sha256
      (base32 "1k2a3vbq96ic4lw72iwp5s3mwwc4xhdffjj584yn6l9637q9j1yd"))
     (modules '((guix build utils)))
     (snippet
      `(begin
         (delete-file-recursively "htslib") #t))))
   (build-system gnu-build-system)
   (inputs
    `(("htslib" ,htslib)
      ("zlib" ,zlib)))
   (arguments
    `(#:tests? #f ; There are no tests to run.
      #:phases
      (modify-phases %standard-phases
        (delete 'configure) ; There is no configure phase.
        ;; The build phase needs overriding the location of htslib.
        (replace 'build
          (lambda* (#:key inputs #:allow-other-keys)
            (let ((htslib-ref (assoc-ref inputs "htslib")))
              (invoke "make"
                      (string-append "HTS_LIB=" htslib-ref "/lib/libhts.a")
                      (string-append "INCLUDES= -I" htslib-ref "/include/htslib")
                      "HTS_HEADERS="    ; No need to check for headers here.
                      (string-append "LIBPATH=-L. -L" htslib-ref "/include"))
              (invoke "g++" "-shared" "-o" "libtabixpp.so" "tabix.o" "-lhts")
              (invoke "ar" "rcs" "libtabixpp.a" "tabix.o"))))
        (replace 'install
          (lambda* (#:key outputs #:allow-other-keys)
            (let* ((out (assoc-ref outputs "out"))
                   (lib (string-append out "/lib"))
                   (bin (string-append out "/bin")))
              (install-file "tabix++" bin)
              (install-file "libtabixpp.so" lib)
              (install-file "libtabixpp.a" lib)
              (install-file "tabix.hpp" (string-append out "/include"))
              (mkdir-p (string-append lib "/pkgconfig"))
              (with-output-to-file (string-append lib "/pkgconfig/tabixpp.pc")
                (lambda _
                  (format #t "prefix=~a~@
                          exec_prefix=${prefix}~@
                          libdir=${exec_prefix}/lib~@
                          includedir=${prefix}/include~@
                          ~@
                          ~@
                          Name: libtabixpp~@
                          Version: ~a~@
                          Description: C++ wrapper around tabix project~@
                          Libs: -L${libdir} -ltabixpp~@
                          Cflags: -I${includedir}~%"
                          out ,version)))
              #t))))))
   (home-page "https://github.com/ekg/tabixpp")
   (synopsis "C++ wrapper around tabix project")
   (description "This is a C++ wrapper around the Tabix project which abstracts
some of the details of opening and jumping in tabix-indexed files.")
   (license license:expat)))

(define-public smithwaterman
  (let ((commit "2610e259611ae4cde8f03c72499d28f03f6d38a7"))
    (package
      (name "smithwaterman")
      (version (git-version "0.0.0" "2" commit))
      (source (origin
        (method git-fetch)
        (uri (git-reference
              (url "https://github.com/ekg/smithwaterman/")
              (commit commit)))
        (file-name (git-file-name name version))
        (sha256
         (base32 "0i9d8zrxpiracw3mxzd9siybpy62p06rqz9mc2w93arajgbk45bs"))))
      (build-system gnu-build-system)
      (arguments
       `(#:tests? #f ; There are no tests to run.
         #:make-flags '("libsw.a" "all")
         #:phases
         (modify-phases %standard-phases
           (delete 'configure) ; There is no configure phase.
           (add-after 'unpack 'patch-source
             (lambda _
               (substitute* "Makefile"
                 (("-c ") "-c -fPIC "))
               #t))
           (add-after 'build 'build-dynamic
             (lambda _
               (invoke "g++"
                       "-shared" "-o" "libsmithwaterman.so"
                       "smithwaterman.o" "SmithWatermanGotoh.o"
                       "disorder.o" "BandedSmithWaterman.o"
                       "LeftAlign.o" "Repeats.o" "IndelAllele.o")))
           (replace 'install
             (lambda* (#:key outputs #:allow-other-keys)
               (let* ((out (assoc-ref outputs "out"))
                      (bin (string-append out "/bin"))
                      (lib (string-append out "/lib")))
                 (install-file "smithwaterman" bin)
                 (for-each
                   (lambda (file)
                     (install-file file (string-append out "/include/smithwaterman")))
                   (find-files "." "\\.h$"))
                 (install-file "libsmithwaterman.so" lib)
                 (install-file "libsw.a" lib)
                 (mkdir-p (string-append lib "/pkgconfig"))
                 (with-output-to-file (string-append lib "/pkgconfig/smithwaterman.pc")
                   (lambda _
                     (format #t "prefix=~a~@
                             exec_prefix=${prefix}~@
                             libdir=${exec_prefix}/lib~@
                             includedir=${prefix}/include/smithwaterman~@
                             ~@
                             ~@
                             Name: smithwaterman~@
                             Version: ~a~@
                             Description: smith-waterman-gotoh alignment algorithm~@
                             Libs: -L${libdir} -lsmithwaterman~@
                             Cflags: -I${includedir}~%"
                             out ,version))))
               #t)))))
      (home-page "https://github.com/ekg/smithwaterman")
      (synopsis "Implementation of the Smith-Waterman algorithm")
      (description "Implementation of the Smith-Waterman algorithm.")
      ;; The licensing terms are unclear: https://github.com/ekg/smithwaterman/issues/9.
      (license (list license:gpl2 license:expat)))))

(define-public multichoose
  (package
    (name "multichoose")
    (version "1.0.3")
    (source (origin
      (method git-fetch)
      (uri (git-reference
            (url "https://github.com/ekg/multichoose/")
            (commit (string-append "v" version))))
      (file-name (git-file-name name version))
      (sha256
       (base32 "0ci5fqvmpamwgxvmyd79ygj6n3bnbl3vc7b6h1sxz58186sm3pfs"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; Tests require node.
       #:phases
       (modify-phases %standard-phases
         (delete 'configure) ; There is no configure phase.
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let ((bin (string-append (assoc-ref outputs "out") "/bin")))
               ;; TODO: There are Python modules for these programs too.
               (install-file "multichoose" bin)
               (install-file "multipermute" bin))
             #t)))))
    (home-page "https://github.com/ekg/multichoose")
    (synopsis "Efficient loopless multiset combination generation algorithm")
    (description "This library implements an efficient loopless multiset
combination generation algorithm which is (approximately) described in
\"Loopless algorithms for generating permutations, combinations, and other
combinatorial configurations.\", G. Ehrlich - Journal of the ACM (JACM),
1973. (Algorithm 7.)")
    (license license:expat)))

(define-public fsom
  (let ((commit "a6ef318fbd347c53189384aef7f670c0e6ce89a3"))
    (package
      (name "fsom")
      (version (git-version "0.0.0" "1" commit))
      (source (origin
        (method git-fetch)
        (uri (git-reference
              (url "https://github.com/ekg/fsom/")
              (commit commit)))
        (file-name (git-file-name name version))
        (sha256
         (base32 "0gw1lpvr812pywg9y546x0h1hhj261xwls41r6kqhddjlrcjc0pi"))))
      (build-system gnu-build-system)
      (arguments
       `(#:tests? #f ; There are no tests to run.
         #:phases
         (modify-phases %standard-phases
           (delete 'configure) ; There is no configure phase.
           (replace 'install
             (lambda* (#:key outputs #:allow-other-keys)
               (let ((bin (string-append (assoc-ref outputs "out") "/bin")))
                 (install-file "fsom" bin))
               #t)))))
      (home-page "https://github.com/ekg/fsom")
      (synopsis "Manage SOM (Self-Organizing Maps) neural networks")
      (description "A tiny C library for managing SOM (Self-Organizing Maps)
neural networks.")
      (license license:gpl3))))

(define-public fastahack
  (package
    (name "fastahack")
    (version "1.0.0")
    (source (origin
      (method git-fetch)
      (uri (git-reference
            (url "https://github.com/ekg/fastahack/")
            (commit (string-append "v" version))))
      (file-name (git-file-name name version))
      (sha256
       (base32 "0rp1blskhzxf7vbh253ibpxbgl9wwgyzf1wbkxndi08d3j4vcss9"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; Unclear how to run tests: https://github.com/ekg/fastahack/issues/15
       #:phases
       (modify-phases %standard-phases
         (delete 'configure) ; There is no configure phase.
           (add-after 'unpack 'patch-source
             (lambda _
               (substitute* "Makefile"
                 (("-c ") "-c -fPIC "))
               #t))
         (add-after 'build 'build-dynamic
           (lambda _
             (invoke "g++"
                     "-shared" "-o" "libfastahack.so"
                     "Fasta.o" "FastaHack.o" "split.o" "disorder.o")))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (lib (string-append out "/lib"))
                    (bin (string-append out "/bin")))
               (mkdir-p (string-append out "/include/fastahack"))
               (for-each
                 (lambda (file)
                   (install-file file (string-append out "/include/fastahack")))
                 (find-files "." "\\.h$"))
               (install-file "fastahack" bin)
               (install-file "libfastahack.so" lib)
               (mkdir-p (string-append lib "/pkgconfig"))
               (with-output-to-file (string-append lib "/pkgconfig/fastahack.pc")
                 (lambda _
                   (format #t "prefix=~a~@
                           exec_prefix=${prefix}~@
                           libdir=${exec_prefix}/lib~@
                           includedir=${prefix}/include/fastahack~@
                           ~@
                           ~@
                           Name: fastahack~@
                           Version: ~a~@
                           Description: Indexing and sequence extraction from FASTA files~@
                           Libs: -L${libdir} -lfastahack~@
                           Cflags: -I${includedir}~%"
                           out ,version))))
             #t)))))
    (home-page "https://github.com/ekg/fastahack")
    (synopsis "Indexing and sequence extraction from FASTA files")
    (description "Fastahack is a small application for indexing and
extracting sequences and subsequences from FASTA files.  The included library
provides a FASTA reader and indexer that can be embedded into applications
which would benefit from directly reading subsequences from FASTA files.  The
library automatically handles index file generation and use.")
    (license (list license:expat license:gpl2))))

(define-public vcflib
  (package
    (name "vcflib")
    (version "1.0.1")
    (source
     (origin
       (method url-fetch)
       (uri (string-append "https://github.com/vcflib/vcflib/releases/"
                           "download/v" version
                           "/vcflib-" version "-src.tar.gz"))
       (sha256
        (base32 "14zzrg8hg8cq9cvq2wdvp21j7nmxxkjrbagw2apd2yqv2kyx42lm"))
       (patches (search-patches "vcflib-use-shared-libraries.patch"))
       (modules '((guix build utils)))
       (snippet
        `(begin
           (substitute* (find-files "." "\\.(h|c)(pp)?$")
             (("\"SmithWatermanGotoh.h\"") "<smithwaterman/SmithWatermanGotoh.h>")
             (("\"convert.h\"") "<smithwaterman/convert.h>")
             (("\"disorder.h\"") "<smithwaterman/disorder.h>")
             (("\"tabix.hpp\"") "<tabix.hpp>")
             (("\"Fasta.h\"") "<fastahack/Fasta.h>"))
           (for-each delete-file-recursively
                     '("fastahack" "filevercmp" "fsom" "googletest" "intervaltree"
                       "libVCFH" "multichoose" "smithwaterman" "tabixpp"))
           #t))))
    (build-system gnu-build-system)
    (inputs
     `(("htslib" ,htslib)
       ("fastahack" ,fastahack)
       ("perl" ,perl)
       ("python" ,python)
       ("smithwaterman" ,smithwaterman)
       ("tabixpp" ,tabixpp)
       ("xz" ,xz)
       ("zlib" ,zlib)))
    (native-inputs
     `(("pkg-config" ,pkg-config)
       ;; Submodules.
       ;; This package builds against the .o files so we need to extract the source.
       ("filevercmp-src" ,(package-source filevercmp))
       ("intervaltree-src" ,(package-source intervaltree))
       ("multichoose-src" ,(package-source multichoose))))
    (arguments
     `(#:tests? #f ; no tests
       #:phases
       (modify-phases %standard-phases
         (add-after 'unpack 'set-flags
           (lambda* (#:key outputs #:allow-other-keys)
             (substitute* "Makefile"
               (("LDFLAGS =")
                (string-append "LDFLAGS = -Wl,-rpath="
                               (assoc-ref outputs "out") "/lib ")))
             (substitute* "filevercmp/Makefile"
               (("-c") "-c -fPIC"))
             #t))
         (delete 'configure)
         (add-after 'unpack 'unpack-submodule-sources
           (lambda* (#:key inputs #:allow-other-keys)
             (let ((unpack (lambda (source target)
                             (mkdir target)
                             (with-directory-excursion target
                               (if (file-is-directory? (assoc-ref inputs source))
                                   (copy-recursively (assoc-ref inputs source) ".")
                                   (invoke "tar" "xvf"
                                           (assoc-ref inputs source)
                                           "--strip-components=1"))))))
               (and
                (unpack "filevercmp-src" "filevercmp")
                (unpack "intervaltree-src" "intervaltree")
                (unpack "multichoose-src" "multichoose")))))
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (let* ((out (assoc-ref outputs "out"))
                    (bin (string-append out "/bin"))
                    (lib (string-append out "/lib")))
               (for-each (lambda (file)
                           (install-file file bin))
                         (find-files "bin" ".*"))
               (install-file "libvcflib.so" lib)
               (install-file "libvcflib.a" lib)
               (for-each
                 (lambda (file)
                   (install-file file (string-append out "/include")))
                 (find-files "include" "\\.h(pp)?$"))
               (mkdir-p (string-append lib "/pkgconfig"))
               (with-output-to-file (string-append lib "/pkgconfig/vcflib.pc")
                 (lambda _
                   (format #t "prefix=~a~@
                           exec_prefix=${prefix}~@
                           libdir=${exec_prefix}/lib~@
                           includedir=${prefix}/include~@
                           ~@
                           ~@
                           Name: libvcflib~@
                           Version: ~a~@
                           Requires: smithwaterman, fastahack~@
                           Description: C++ library for parsing and manipulating VCF files~@
                           Libs: -L${libdir} -lvcflib~@
                           Cflags: -I${includedir}~%"
                           out ,version))))
             #t)))))
    (home-page "https://github.com/vcflib/vcflib/")
    (synopsis "Library for parsing and manipulating VCF files")
    (description "Vcflib provides methods to manipulate and interpret
sequence variation as it can be described by VCF.  It is both an API for parsing
and operating on records of genomic variation as it can be described by the VCF
format, and a collection of command-line utilities for executing complex
manipulations on VCF files.")
    (license license:expat)))

(define-public freebayes
  (let ((commit "3ce827d8ebf89bb3bdc097ee0fe7f46f9f30d5fb")
        (revision "1")
        (version "1.0.2"))
    (package
      (name "freebayes")
      (version (git-version version revision commit))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                      (url "https://github.com/ekg/freebayes")
                      (commit commit)))
                (file-name (git-file-name name version))
                (sha256
                 (base32 "1sbzwmcbn78ybymjnhwk7qc5r912azy5vqz2y7y81616yc3ba2a2"))))
      (build-system gnu-build-system)
      (inputs
       `(("bamtools" ,bamtools)
         ("htslib" ,htslib)
         ("zlib" ,zlib)))
      (native-inputs
       `(("bc" ,bc)                     ; Needed for running tests.
         ("samtools" ,samtools)         ; Needed for running tests.
         ("parallel" ,parallel)         ; Needed for running tests.
         ("perl" ,perl)                 ; Needed for running tests.
         ("procps" ,procps)             ; Needed for running tests.
         ("python" ,python-2)           ; Needed for running tests.
         ("vcflib-src" ,(package-source vcflib))
         ;; These are submodules for the vcflib version used in freebayes.
         ;; This package builds against the .o files so we need to extract the source.
         ("tabixpp-src" ,(package-source tabixpp))
         ("smithwaterman-src" ,(package-source smithwaterman))
         ("multichoose-src" ,(package-source multichoose))
         ("fsom-src" ,(package-source fsom))
         ("filevercmp-src" ,(package-source filevercmp))
         ("fastahack-src" ,(package-source fastahack))
         ("intervaltree-src" ,(package-source intervaltree))
         ;; These submodules are needed to run the tests.
         ("bash-tap-src" ,(package-source bash-tap))
         ("test-simple-bash-src"
          ,(origin
             (method git-fetch)
             (uri (git-reference
                   (url "https://github.com/ingydotnet/test-simple-bash/")
                   (commit "124673ff204b01c8e96b7fc9f9b32ee35d898acc")))
             (file-name "test-simple-bash-src-checkout")
             (sha256
              (base32 "043plp6z0x9yf7mdpky1fw7zcpwn1p47px95w9mh16603zqqqpga"))))))
      (arguments
       `(#:make-flags
         (list "CC=gcc"
               (string-append "BAMTOOLS_ROOT="
                              (assoc-ref %build-inputs "bamtools")))
         #:test-target "test"
         #:phases
         (modify-phases %standard-phases
           (delete 'configure)
           (add-after 'unpack 'fix-tests
             (lambda _
               (substitute* "test/t/01_call_variants.t"
                 (("grep -P \"\\(\\\\t500\\$\\|\\\\t11000\\$\\|\\\\t1000\\$\\)\"")
                  "grep -E '	(500|11000|1000)$'"))
               #t))
           (add-after 'unpack 'unpack-submodule-sources
             (lambda* (#:key inputs #:allow-other-keys)
               (let ((unpack (lambda (source target)
                               (with-directory-excursion target
                                 (if (file-is-directory? (assoc-ref inputs source))
                                     (copy-recursively (assoc-ref inputs source) ".")
                                     (invoke "tar" "xvf"
                                             (assoc-ref inputs source)
                                             "--strip-components=1"))))))
                 (and
                  (unpack "vcflib-src" "vcflib")
                  (unpack "fastahack-src" "vcflib/fastahack")
                  (unpack "filevercmp-src" "vcflib/filevercmp")
                  (unpack "fsom-src" "vcflib/fsom")
                  (unpack "intervaltree-src" "vcflib/intervaltree")
                  (unpack "multichoose-src" "vcflib/multichoose")
                  (unpack "smithwaterman-src" "vcflib/smithwaterman")
                  (unpack "tabixpp-src" "vcflib/tabixpp")
                  (unpack "test-simple-bash-src" "test/test-simple-bash")
                  (unpack "bash-tap-src" "test/bash-tap")))))
           (add-after 'unpack-submodule-sources 'fix-makefiles
             (lambda _
               ;; We don't have the .git folder to get the version tag from.
               (substitute* "vcflib/Makefile"
                 (("^GIT_VERSION.*")
                  (string-append "GIT_VERSION = v" ,version)))
               (substitute* "src/Makefile"
                 (("-I\\$\\(BAMTOOLS_ROOT\\)/src")
                  "-I$(BAMTOOLS_ROOT)/include/bamtools"))
               #t))
           (add-before 'build 'build-tabixpp-and-vcflib
             (lambda* (#:key inputs make-flags #:allow-other-keys)
               (with-directory-excursion "vcflib"
                 (with-directory-excursion "tabixpp"
                   (apply invoke "make"
                          (string-append "HTS_LIB="
                                         (assoc-ref inputs "htslib")
                                         "/lib/libhts.a")
                          make-flags))
                 (apply invoke "make"
                        (string-append "CFLAGS=-Itabixpp")
                        "all"
                        make-flags))))
           (replace 'install
             (lambda* (#:key outputs #:allow-other-keys)
               (let ((bin (string-append (assoc-ref outputs "out") "/bin")))
                 (install-file "bin/freebayes" bin)
                 (install-file "bin/bamleftalign" bin))
               #t)))))
      (home-page "https://github.com/ekg/freebayes")
      (synopsis "Haplotype-based variant detector")
      (description "FreeBayes is a Bayesian genetic variant detector designed to
find small polymorphisms, specifically SNPs (single-nucleotide polymorphisms),
indels (insertions and deletions), MNPs (multi-nucleotide polymorphisms), and
complex events (composite insertion and substitution events) smaller than the
length of a short-read sequencing alignment.")
      (license license:expat))))

(define-public samblaster
  (package
    (name "samblaster")
    (version "0.1.24")
    (source (origin
      (method git-fetch)
      (uri (git-reference
            (url "https://github.com/GregoryFaust/samblaster")
            (commit (string-append "v." version))))
      (file-name (git-file-name name version))
      (sha256
       (base32
        "0iv2ddfw8363vb2x8gr3p8g88whb6mb9m0pf71i2cqsbv6jghap7"))))
    (build-system gnu-build-system)
    (arguments
     `(#:tests? #f ; there are none
       #:phases
       (modify-phases %standard-phases
         (delete 'configure) ; There is no configure phase.
         (replace 'install
           (lambda* (#:key outputs #:allow-other-keys)
             (install-file "samblaster"
                           (string-append (assoc-ref outputs "out") "/bin"))
             #t)))))
    (home-page "https://github.com/GregoryFaust/samblaster")
    (synopsis "Mark duplicates in paired-end SAM files")
    (description "Samblaster is a fast and flexible program for marking
duplicates in read-id grouped paired-end SAM files.  It can also optionally
output discordant read pairs and/or split read mappings to separate SAM files,
and/or unmapped/clipped reads to a separate FASTQ file. When marking
duplicates, samblaster will require approximately 20MB of memory per 1M read
pairs.")
    (license license:expat)))

(define-public r-velocyto
  (let ((commit "d7790346cb99f49ab9c2b23ba70dcf9d2c9fc350")
        (revision "1"))
    (package
      (name "r-velocyto")
      (version (git-version "0.6" revision commit))
      (source
       (origin
         (method git-fetch)
         (uri (git-reference
               (url "https://github.com/velocyto-team/velocyto.R")
               (commit commit)))
         (file-name (git-file-name name version))
         (sha256
          (base32
           "16wqf70j7rd7pay2q513iyz12i8n9vrpg1bisah4lddbcpx5dz1n"))))
      (build-system r-build-system)
      (inputs
       `(("boost" ,boost)))
      (propagated-inputs
       `(("r-hdf5r" ,r-hdf5r)
         ("r-mass" ,r-mass)
         ("r-mgcv" ,r-mgcv)
         ("r-pcamethods" ,r-pcamethods)
         ("r-rcpp" ,r-rcpp)
         ("r-rcpparmadillo" ,r-rcpparmadillo)
         ;; Suggested packages
         ("r-rtsne" ,r-rtsne)
         ("r-cluster" ,r-cluster)
         ("r-abind" ,r-abind)
         ("r-h5" ,r-h5)
         ("r-biocgenerics" ,r-biocgenerics)
         ("r-genomicalignments" ,r-genomicalignments)
         ("r-rsamtools" ,r-rsamtools)
         ("r-edger" ,r-edger)
         ("r-igraph" ,r-igraph)))
      (home-page "https://velocyto.org")
      (synopsis "RNA velocity estimation in R")
      (description
       "This package provides basic routines for estimation of gene-specific
transcriptional derivatives and visualization of the resulting velocity
patterns.")
      (license license:gpl3))))

(define-public methyldackel
  (package
    (name "methyldackel")
    (version "0.5.1")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/dpryan79/MethylDackel")
                    (commit version)))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "1sfhf2ap75qxpnmy1ifgmxqs18rq8mah9mcgkby73vc6h0sw99ws"))))
    (build-system gnu-build-system)
    (arguments
     `(#:test-target "test"
       #:make-flags
       (list "CC=gcc"
             (string-append "prefix="
                            (assoc-ref %outputs "out") "/bin/"))
       #:phases
       (modify-phases %standard-phases
         (replace 'configure
           (lambda* (#:key outputs #:allow-other-keys)
             (substitute* "Makefile"
               (("-lhts ") "-lhts -lBigWig ")
               (("install MethylDackel \\$\\(prefix\\)" match)
                (string-append "install -d $(prefix); " match)))
             #t)))))
    (inputs
     `(("curl" ,curl) ; XXX: needed by libbigwig
       ("htslib" ,htslib-1.9)
       ("libbigwig" ,libbigwig)
       ("zlib" ,zlib)))
    ;; Needed for tests
    (native-inputs
     `(("python" ,python-wrapper)))
    (home-page "https://github.com/dpryan79/MethylDackel")
    (synopsis "Universal methylation extractor for BS-seq experiments")
    (description
     "MethylDackel will process a coordinate-sorted and indexed BAM or CRAM
file containing some form of BS-seq alignments and extract per-base
methylation metrics from them.  MethylDackel requires an indexed fasta file
containing the reference genome as well.")
    ;; See https://github.com/dpryan79/MethylDackel/issues/85
    (license license:expat)))

;; This package bundles PCRE 8.02 and cannot be built with the current
;; version.
(define-public phast
  (package
    (name "phast")
    (version "1.5")
    (source (origin
              (method git-fetch)
              (uri (git-reference
                    (url "https://github.com/CshlSiepelLab/phast")
                    (commit (string-append "v" version))))
              (file-name (git-file-name name version))
              (sha256
               (base32
                "10lpbllvny923jjbbyrpxahhd1m5h7sbj9gx7rd123rg10mlidki"))))
    (build-system gnu-build-system)
    (arguments
     `(#:make-flags
       (list "CC=gcc"
             (string-append "DESTDIR=" (assoc-ref %outputs "out")))
       #:phases
       (modify-phases %standard-phases
         (replace 'configure
           (lambda* (#:key inputs outputs #:allow-other-keys)
             ;; Fix syntax
             (substitute* "test/Makefile"
               (("        ") "	"))
             (substitute* "Makefile"
               (("CLAPACKPATH=/usr/lib")
                (string-append "CLAPACKPATH="
                               (assoc-ref inputs "clapack") "/lib")))
             ;; Renaming the libraries is not necessary with our version of
             ;; CLAPACK.
             (substitute* "src/lib/Makefile"
               (("ifdef CLAPACKPATH") "ifdef UNNECESSARY"))
             (substitute* "src/make-include.mk"
               (("-lblaswr") "-lblas")
               (("-ltmg") "-ltmglib")
               (("liblapack.a") "liblapack.so")
               (("libblas.a") "libblas.so")
               (("libf2c.a") "libf2c.so"))
             (substitute* "src/Makefile"
               (("/opt") "/share")
               (("/usr/") "/"))
             #t))
         (replace 'check
           (lambda _
             (setenv "PATH"
                     (string-append (getcwd) "/bin:" (getenv "PATH")))
             ;; Disable broken test
             (substitute* "test/Makefile"
               ((".*if.*hmrc_summary" m) (string-append "#" m)))
             ;; Only run the msa_view tests because the others fail for
             ;; unknown reasons.
             (invoke "make" "-C" "test" "msa_view"))))))
    (inputs
     `(("clapack" ,clapack)))
    (native-inputs
     `(("perl" ,perl)))
    (home-page "http://compgen.cshl.edu/phast/")
    (synopsis "Phylogenetic analysis with space/time models")
    (description
     "Phylogenetic Analysis with Space/Time models (PHAST) is a collection of
command-line programs and supporting libraries for comparative and
evolutionary genomics.  Best known as the search engine behind the
Conservation tracks in the University of California, Santa Cruz (UCSC) Genome
Browser, PHAST also includes several tools for phylogenetic modeling,
functional element identification, as well as utilities for manipulating
alignments, trees and genomic annotations.")
    (license license:bsd-3)))

(define-public python-gffutils
  ;; The latest release is older more than a year than the latest commit
  (let ((commit "4034c54600813b1402945e12faa91b3a53162cf1")
        (revision "1"))
    (package
      (name "python-gffutils")
      (version (git-version "0.9" revision commit))
      (source
       (origin
         (method git-fetch)
         (uri (git-reference
               (url "https://github.com/daler/gffutils")
               (commit commit)))
         (file-name (git-file-name name version))
         (sha256
          (base32
           "1rwafjdnbir5wnk0ap06ww4lra3p5frhy7mfs03rlldgfnwxymsn"))))
      (build-system python-build-system)
      (arguments
       `(#:phases
         (modify-phases %standard-phases
           (replace 'check
             (lambda _
               ;; Tests need to access the HOME directory
               (setenv "HOME" "/tmp")
               (invoke "nosetests" "-a" "!slow")))
           (add-after 'unpack 'make-gz-files-writable
             (lambda _
               (for-each make-file-writable
                         (find-files "." "\\.gz"))
               #t)))))
      (propagated-inputs
       `(("python-argcomplete" ,python-argcomplete)
         ("python-argh" ,python-argh)
         ("python-biopython" ,python-biopython)
         ("python-pybedtools" ,python-pybedtools)
         ("python-pyfaidx" ,python-pyfaidx)
         ("python-simplejson" ,python-simplejson)
         ("python-six" ,python-six)))
      (native-inputs
       `(("python-nose" , python-nose)))
      (home-page "https://github.com/daler/gffutils")
      (synopsis "Tool for manipulation of GFF and GTF files")
      (description
       "python-gffutils is a Python package for working with and manipulating
the GFF and GTF format files typically used for genomic annotations.  The
files are loaded into a SQLite database, allowing much more complex
manipulation of hierarchical features (e.g., genes, transcripts, and exons)
than is possible with plain-text methods alone.")
      (license license:expat))))

(define-public libsbml
  (package
    (name "libsbml")
    (version "5.18.0")
    (source (origin
              (method url-fetch)
              (uri (string-append "mirror://sourceforge/sbml/libsbml/"
                                  version "/stable/libSBML-"
                                  version "-core-src.tar.gz"))
              (sha256
               (base32
                "0slkagrk3nfi2qsksv6b1brj6zhx4bj4bkib2sdycvrcd10ql2lh"))))
    (build-system cmake-build-system)
    (arguments
     `(#:test-target "test"
       #:configure-flags
       (list "-DWITH_CHECK=ON"
             (string-append "-DLIBXML_LIBRARY="
                            (assoc-ref %build-inputs "libxml2")
                            "/lib/libxml2.so")
             (string-append "-DLIBXML_INCLUDE_DIR="
                            (assoc-ref %build-inputs "libxml2")
                            "/include/libxml2"))))
    (propagated-inputs
     `(("libxml2" ,libxml2)))
    (native-inputs
     `(("check" ,check-0.14)
       ("swig" ,swig)))
    (home-page "http://sbml.org/Software/libSBML")
    (synopsis "Process SBML files and data streams")
    (description "LibSBML is a library to help you read, write, manipulate,
translate, and validate SBML files and data streams.  The @dfn{Systems Biology
Markup Language} (SBML) is an interchange format for computer models of
biological processes.  SBML is useful for models of metabolism, cell
signaling, and more.  It continues to be evolved and expanded by an
international community.")
    (license license:lgpl2.1+)))

(define-public grocsvs
  ;; The last release is out of date and new features have been added.
  (let ((commit "ecd956a65093a0b2c41849050e4512d46fecea5d")
        (revision "1"))
    (package
      (name "grocsvs")
      (version (git-version "0.2.6.1" revision commit))
      (source (origin
                (method git-fetch)
                (uri (git-reference
                       (url "https://github.com/grocsvs/grocsvs")
                       (commit commit)))
                (file-name (git-file-name name version))
                (sha256
                 (base32 "14505725gr7qxc17cxxf0k6lzcwmgi64pija4mwf29aw70qn35cc"))
                (patches (search-patches "grocsvs-dont-use-admiral.patch"))))
      (build-system python-build-system)
      (arguments
       `(#:tests? #f            ; No test suite.
         #:python ,python-2))   ; Only python-2 supported.
      (inputs
       `(("python2-h5py" ,python2-h5py)
         ("python2-ipython-cluster-helper" ,python2-ipython-cluster-helper)
         ("python2-networkx" ,python2-networkx)
         ("python2-psutil" ,python2-psutil)
         ("python2-pandas" ,python2-pandas)
         ("python2-pybedtools" ,python2-pybedtools)
         ("python2-pyfaidx" ,python2-pyfaidx)
         ("python2-pygraphviz" ,python2-pygraphviz)
         ("python2-pysam" ,python2-pysam)
         ("python2-scipy" ,python2-scipy)))
      (home-page "https://github.com/grocsvs/grocsvs")
      (synopsis "Genome-wide reconstruction of complex structural variants")
      (description
       "@dfn{Genome-wide Reconstruction of Complex Structural Variants}
(GROC-SVs) is a software pipeline for identifying large-scale structural
variants, performing sequence assembly at the breakpoints, and reconstructing
the complex structural variants using the long-fragment information from the
10x Genomics platform.")
       (license license:expat))))